Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	2,717,858	G→A	6.6%	A296T (GCT→ACT)	blt →	efflux transporter (promiscuous, acetylated polyamines efflux)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,717,858	0	G	A	6.6%	199.1 / 2.2	60	A296T (GCT→ACT)	blt	efflux transporter (promiscuous, acetylated polyamines efflux)
Reads supporting (aligned to +/- strand):  ref base G (31/25);  new base A (2/2);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGATATTGCAGCCATTATTACGATTAGTTCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATCCAATATGGCCGGTAACCAGCAGGGTTTCGTAG  >  BS168_reference_genome/2717723‑2717996                                                                                                                                        |                                                                                                                                           aaaGATATTGCAGCCATTATTACGATTAGTTCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCtt                                                                                                                                         >  2:265151/1‑139 (MQ=255)  aaGATATTGCAGCCATTATTACGATTAGTTCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCttt                                                                                                                                        <  3:166871/139‑1 (MQ=255)   aGATATTGCAGCCATTATTACGATTAGTTCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTc                                                                                                                                       <  2:251747/139‑1 (MQ=255)     atatTGCAGCCATTATTACGATTAGTTCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCgt                                                                                                                                     <  1:139459/139‑1 (MQ=255)     atatTGCAGCCATTATTACGATTAGTTCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCgt                                                                                                                                     <  1:26519/139‑1 (MQ=255)        ttGCAGCCATTATTACGATTAGTTCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCgtgt                                                                                                                                   <  1:178111/138‑1 (MQ=255)           cAGCCATTATTACGATTAGTTCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTa                                                                                                                                <  1:220463/138‑1 (MQ=255)                attattACGATTAGTTCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGACTTTCGTGTCTACTGTTa                                                                                                                          >  1:251831/1‑139 (MQ=255)                attattACGATTAGTTCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGACTTTCGTGTCTACTGTTa                                                                                                                          >  1:256859/1‑139 (MQ=255)                      aCGATTAGTTCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCa                                                                                                                     <  1:59022/138‑1 (MQ=255)                        gATTAGTTCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGAGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGa                                                                                                                  >  2:266476/1‑139 (MQ=255)                            aGTTCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGAtttt                                                                                                              >  1:334974/1‑139 (MQ=255)                            aGTTCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGAtttt                                                                                                              >  3:137917/1‑139 (MQ=255)                              ttCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGAttttt                                                                                                             >  4:4171/1‑138 (MQ=255)                              ttCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTa                                                                                                            <  2:301745/139‑1 (MQ=255)                               tCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTaa                                                                                                           >  8:96476/1‑139 (MQ=255)                                ccATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGTAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTaa                                                                                                           <  5:205334/138‑1 (MQ=255)                                   ttgttgCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCATGATTTTTAACTGt                                                                                                       >  7:302876/1‑139 (MQ=255)                                   ttgttgCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGt                                                                                                       >  3:112345/1‑139 (MQ=255)                                     gttgCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGtt                                                                                                      <  4:112345/138‑1 (MQ=255)                                        gCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAGCAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGc                                                                                                  >  2:12160/1‑139 (MQ=255)                                        gCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGc                                                                                                  >  5:295147/1‑139 (MQ=255)                                          ggTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTGTACTGTTATGTCAGGATTTTTAACTGTTTTGCtt                                                                                                <  7:96476/139‑1 (MQ=255)                                              gTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTaa                                                                                            >  7:315763/1‑139 (MQ=255)                                                tATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCt                                                                                                                                          >  4:86688/1‑91 (MQ=255)                                                tATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCt                                                                                                                                          <  3:86688/91‑1 (MQ=255)                                                      aGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGtttt                                                                                    >  4:127347/1‑139 (MQ=255)                                                                 tCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGc                                                                         >  5:44301/1‑139 (MQ=255)                                                                 tCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGc                                                                         >  3:207691/1‑139 (MQ=255)                                                                          tGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGAttt                                                                >  7:66196/1‑139 (MQ=255)                                                                             tCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCt                                                             <  3:127347/139‑1 (MQ=255)                                                                                 ctaaCTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTGTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGt                                                         <  7:102166/137‑1 (MQ=255)                                                                                 caaACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGt                                                         >  6:104864/1‑139 (MQ=255)                                                                                 caaACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGt                                                         <  3:231531/139‑1 (MQ=255)                                                                                 caaACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGt                                                         <  2:216243/139‑1 (MQ=255)                                                                                 caaACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGt                                                         <  8:302876/139‑1 (MQ=255)                                                                                       tGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGt                                                                       <  3:201281/119‑1 (MQ=255)                                                                                       tGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGt                                                                       >  4:201281/1‑119 (MQ=255)                                                                                         gagagaAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCt                                                  >  8:250596/1‑138 (MQ=255)                                                                                              aaaaGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGt                                                         >  1:90032/1‑126 (MQ=255)                                                                                              aaaaGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGt                                                         <  2:90032/126‑1 (MQ=255)                                                                                              aaaaGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCg                                            <  7:106659/139‑1 (MQ=255)                                                                                               aaaGAATGATTCAGCTGTGCTTAATAACAGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTTCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCg                                            <  8:292695/138‑1 (MQ=255)                                                                                                aaGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCtt                                                 <  8:186310/132‑1 (MQ=255)                                                                                                aaGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCtt                                                 >  7:186310/1‑132 (MQ=255)                                                                                                       aTTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCTTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTa                                    <  7:250596/138‑1 (MQ=255)                                                                                                       aTTCAGCTGTGCTTAATAACCGGTGCGATCTTGACTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTAt                                   <  2:256859/139‑1 (MQ=255)                                                                                                       aTTCAGCTGTGCTTAATAACCGGTGCGATCTTGACTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTAt                                   <  2:251831/139‑1 (MQ=255)                                                                                                        ttCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATc                                  >  1:91870/1‑139 (MQ=255)                                                                                                             cTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATCCAata                             >  2:284279/1‑139 (MQ=255)                                                                                                                tgCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATCCAATATgg                          >  6:226549/1‑139 (MQ=255)                                                                                                                tgCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGGTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATCCAatat                            <  4:6248/137‑1 (MQ=255)                                                                                                                  cTTAAAAACCGGAGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATATTTAACTGTTATGCTAGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGAACGGCCTAAACAGCACATTTATCCAATATCGcc                        >  2:1828/1‑139 (MQ=255)                                                                                                                     aataaCCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATCCAATATGGCCgg                      >  5:7012/1‑138 (MQ=255)                                                                                                                     aataaCCGGTGCGATCTTGGCTTTCGTGTCTACTGTGATGTCAGGATTTTTAACTGTTTTGCTTGTATCTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTCACCGCTCAGTTATCCAATATGGCCgg                      >  2:101890/1‑138 (MQ=255)                                                                                                                          ccGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCt                                                             <  2:19014/94‑1 (MQ=255)                                                                                                                          ccGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCt                                                             >  1:19014/1‑94 (MQ=255)                                                                                                                          ccGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATCCAATATGGCCGGTAAc                  >  4:141646/1‑137 (MQ=255)                                                                                                                            ggTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATCCAATATGGCCGGTAACcag               >  1:293937/1‑138 (MQ=255)                                                                                                                                cGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATCCAATATGGCCGGTAACCAGCAggg          >  8:104323/1‑139 (MQ=255)                                                                                                                                        gCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATCCAATATGGCCGGTAACcag               >  6:269483/1‑126 (MQ=255)                                                                                                                                        gCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATCCAATATGGCCGGTAACcag               <  5:269483/126‑1 (MQ=255)                                                                                                                                        gCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATCCAATATGGCCGGTAACCAGCAGGGTTTCGTAg  <  5:226549/139‑1 (MQ=255)                                                                                                                                        gCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATCCAATATGGCCGGTAACCAGCAGGGTTTCGTAg  <  4:137917/139‑1 (MQ=255)                                                                                                                                        gCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATCCAATATGGCCGGTAACCAGCAGGGTTTCGTAg  <  8:66196/139‑1 (MQ=255)                                                                                                                                        gCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATCCAATATGGCCGGTAACCAGCAGGGTTTCGTAg  <  1:12160/139‑1 (MQ=255)                                                                                                                                        gCTTTCGTGTCTACTGTTATGTCAGGATTATTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATCCAATATGGCCGGTAACCAGCAGGGTTTCGTAg  <  4:141161/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                           AAAGATATTGCAGCCATTATTACGATTAGTTCCATTGTTGCGGTAGTTATTCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGATCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGATTTGCTACGTCCGGCCTTAACCGCTCATTTATCCAATATGGCCGGTAACCAGCAGGGTTTCGTAG  >  BS168_reference_genome/2717723‑2717996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑