| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 126,612 | A→T | 5.3% | K351* (AAA→TAA) | rpoC → | RNA polymerase (beta' subunit) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 126,612 | 0 | A | T | 5.3% | 283.9 / 3.7 | 89 | K351* (AAA→TAA) | rpoC | RNA polymerase (beta' subunit) |
| Reads supporting (aligned to +/- strand): ref base A (49/35); new base T (3/2); total (52/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGTCCTGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCGAAACGCAAAATTGAGCGCGTACAGCCT > BS168_reference_genome/126478‑126746 | cAGGTCCTGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCGTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAAt > 5:84322/1‑139 (MQ=255) gTCCTGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAACACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTGCTCATTTGAAAATGTa > 7:256593/1‑139 (MQ=255) gTCCTGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGCCGTTCGGTAATGGTTGTTGGTCGTCATTTGTAAATGTa > 6:233549/1‑139 (MQ=255) gTCCTGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGt > 2:186824/1‑138 (MQ=255) gTCCTGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTa > 1:105572/1‑139 (MQ=255) gTCCTGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTa > 7:114921/1‑139 (MQ=255) gTCCTGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTa > 6:329772/1‑139 (MQ=255) gTCCTGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTa > 6:319777/1‑139 (MQ=255) gTCCTGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTa > 5:309820/1‑139 (MQ=255) ccTGGAAACAGCCCGTTAAAATCTCTTTCTCAGATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCATACCCTTCTTGGTAACCGTGTCGCTTACTCCGGACGTTCTGTCATCGTTGTTGGTCCTCATTTGAAAATGTAcc > 2:11319/1‑139 (MQ=255) ccTGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTTGTACTCATTTGAAAATGTAc > 2:324276/1‑138 (MQ=255) ccTGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGa < 3:178082/130‑1 (MQ=255) ccTGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGa > 4:178082/1‑130 (MQ=255) ccTGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTAc > 6:266187/1‑138 (MQ=255) ccTGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTACTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTAc > 5:129138/1‑138 (MQ=255) tGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCaa > 3:87535/1‑139 (MQ=255) gAAACAGACCGTTAAAATCTCTTTCTCACCTGCTGTAAGGGAAGCAAGGGCGTTTCCCTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGATGTTGGTCCTCATTTGAAAATGTACCAAt < 1:364782/138‑1 (MQ=255) aaCAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCg < 6:309820/139‑1 (MQ=255) cAGCCCGTTAAAATCTCTATCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAACCCTTCTTGGTAAACGTGTCGATTACTCCGGCCGGAGGGTAATCGTTGGTGGTCCTCTGTTGAAAATGTACCAATGCGGa > 2:119262/1‑139 (MQ=255) aGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGaaaa < 6:121499/124‑1 (MQ=255) aGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGAt > 4:65144/1‑139 (MQ=255) aGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGAt > 6:84235/1‑139 (MQ=255) aGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCCATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGaaaa > 5:121499/1‑124 (MQ=255) cccGTTAAAATCTCGTTCGCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTGAAAACCGTCTTGGTTAACGTGTCGGGTTCTCCGGGCGGTCTGTAATCGGTGTTGGTCCGCATTTGAAAATGTTCCAATGCGGATTa > 4:280062/2‑139 (MQ=255) aCCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAATATGTACCAATGCGGATTa > 7:334648/1‑139 (MQ=255) aCCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTa > 4:270906/1‑139 (MQ=255) aCCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTa > 7:81508/1‑139 (MQ=255) cGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCTTCAAAACCTTCTTGGTAAACGTGGCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTAcc < 7:34964/139‑1 (MQ=255) gTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGTCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCg < 3:260734/139‑1 (MQ=255) gTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTTGTCCTCATTTGAAAATGTACCAATGCGGATTACCg < 1:324276/139‑1 (MQ=255) ttAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGTAAATGTACCAATGCGGATTACCg > 7:233190/1‑138 (MQ=255) ttAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCAGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGTAAATGTACCAATGCGGATTACCg > 7:227832/1‑138 (MQ=255) tAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACTTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGa < 5:225567/138‑1 (MQ=255) aaaaTCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGaa < 3:152098/138‑1 (MQ=255) tctTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAATGAAATg > 1:324324/1‑139 (MQ=255) tctTTATGACATGGTGAAAGGGAAGCAACGCCGTTTCCGGCAAAACCTTAATGGTAAACGTGTCGATTACTCCGGACCTTGGGTAAACGTTGTTTGTCCGCATTTTAAAATTTTACAATTCGGATTACCCAATGaaaag > 4:143794/1‑137 (MQ=255) tttCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAAt < 5:329772/136‑1 (MQ=255) tttCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGc > 1:270232/1‑139 (MQ=255) tttCTCACATGCGGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAAt < 8:114921/136‑1 (MQ=255) caTGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGaa < 5:65435/139‑1 (MQ=255) gCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACtt > 1:307192/1‑139 (MQ=255) aaaGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTc > 6:14209/1‑138 (MQ=255) aaaGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCa < 5:290081/139‑1 (MQ=255) aaaGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTAACAAAGCGGATTACCGAAGGAAATGGCACTTGATCTTTTc > 4:85199/1‑138 (MQ=255) aaaGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCAGTAATCGTTGTTGGTCCTCATTTGAAAATGTAACAATGCGGATTACCGAAGGAAATGGCACTTGAACttttg > 6:51941/1‑137 (MQ=255) gAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCt > 2:104927/1‑139 (MQ=255) gCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCaa < 4:33980/132‑1 (MQ=255) gCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCaa > 3:294815/1‑132 (MQ=255) gCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCaa < 4:294815/132‑1 (MQ=255) gCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCaa > 3:33980/1‑132 (MQ=255) gCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTc > 6:4582/1‑139 (MQ=255) aaGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATTGCACTTGAACTTTTCAAACCTTTAGt < 7:349880/139‑1 (MQ=255) aGGCCGTTTCCGTCAAAACCTTCTTTGTATACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGTAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGtt < 8:227832/139‑1 (MQ=255) aGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGTAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGtt < 8:233190/139‑1 (MQ=255) ttCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGa > 3:134841/1‑136 (MQ=255) aaCGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGc > 5:254900/1‑139 (MQ=255) aCGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCt > 5:81229/1‑139 (MQ=255) cGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCTATGCGGATTACCGAAGGAAATGGCACTTTAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTc < 3:158518/139‑1 (MQ=255) cGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGTACTTGTTGAAAAAGGTCTTGCTc < 6:129138/139‑1 (MQ=255) cGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTc < 5:84235/139‑1 (MQ=255) cGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTc < 3:270906/139‑1 (MQ=255) cGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTc < 5:218620/139‑1 (MQ=255) cGTGTCGATTACTCCGGACGTTCTGTAATCGTTGGTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTc < 2:274133/139‑1 (MQ=255) tgtCGATTACTCCGGACGTTCTGTATTCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGATCTTGTTGAAAAAGGTCTTGCTcac < 4:109328/139‑1 (MQ=255) tgtCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTcac < 4:59344/139‑1 (MQ=255) tgtCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTcac < 5:266187/139‑1 (MQ=255) tgtCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTcac < 5:14209/139‑1 (MQ=255) tgtCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTCCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTcac < 7:316337/139‑1 (MQ=255) tCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCacaa > 1:225014/1‑139 (MQ=255) tCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCacaa > 1:104023/1‑139 (MQ=255) tCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCacaa > 6:161241/1‑139 (MQ=255) gATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCacaaca > 4:196334/1‑139 (MQ=255) tACTCCGGACGTTCTGTAATCGTTGTTGGTCTTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACAtt > 8:26935/1‑138 (MQ=255) cGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTcac > 7:274653/1‑127 (MQ=255) cGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTcac < 8:274653/127‑1 (MQ=255) aCGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCg < 2:270232/139‑1 (MQ=255) gTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCGaa < 6:236255/139‑1 (MQ=255) tCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCGAAAc > 2:116006/1‑139 (MQ=255) cTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGaa < 5:336053/68‑1 (MQ=255) cTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGaa > 6:336053/1‑68 (MQ=255) cTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCGAAACg > 4:298809/1‑139 (MQ=255) cTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCGAAACg > 3:339273/1‑139 (MQ=255) cggtgttgGTCCTCATTTGAATATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAATAGGTCTTGCTCACAACATTAAGAGTGCGAAACGCAAAAt < 8:256593/135‑1 (MQ=255) gttgGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCGAACCGCAAAATTGAgc > 4:81879/1‑139 (MQ=255) gttgGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCGAAACGCAAAATTGAgc > 1:275790/1‑139 (MQ=255) gttgGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCGAAACGCAAAATTGAgc > 2:185862/1‑139 (MQ=255) gttgGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCGAAACGCAAAATTGAgc > 2:340341/1‑139 (MQ=255) gttgGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCGAAACGCAAAATTGAg < 3:298809/138‑1 (MQ=255) tCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCGAAACGCAAAATTGAGCGCGTa < 5:4582/139‑1 (MQ=255) tCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGATATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCGAAACGCAAAATTGAGCGCGTa < 1:333011/139‑1 (MQ=255) cTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCGAAACGCAAAATTGAGCGCGTAc < 1:185862/138‑1 (MQ=255) cATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCGAAACGCAAAATTGAGCGCGTACAGc > 7:174255/1‑139 (MQ=255) tttGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCGAAACGCAAAATTGAGCGCGTACAGCCt < 1:36020/139‑1 (MQ=255) | CAGGTCCTGGAAACAGACCGTTAAAATCTCTTTCTCACATGCTGAAAGGGAAGCAAGGCCGTTTCCGTCAAAACCTTCTTGGTAAACGTGTCGATTACTCCGGACGTTCTGTAATCGTTGTTGGTCCTCATTTGAAAATGTACCAATGCGGATTACCGAAGGAAATGGCACTTGAACTTTTCAAACCTTTCGTTATGAAAGAACTTGTTGAAAAAGGTCTTGCTCACAACATTAAGAGTGCGAAACGCAAAATTGAGCGCGTACAGCCT > BS168_reference_genome/126478‑126746 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |