Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 3,016,944 G→T 10.0% T231N (ACC→AAC)  ytxK ← putative nucleic acid methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome3,016,9440GT10.0% 131.6 / 3.0 43T231N (ACC→AAC) ytxKputative nucleic acid methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (21/17);  new base T (2/3);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAAAATAGATTTTGGCAGCTGCAACAGTGCGTTGATATGAACCTTATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGCCTTCGTCAGCTTTGAGCTCAAACGCTTCTGCTCCTTCGTCATTCGGATAATAGCCGACTGGCAGATCACAAATGAC  >  BS168_reference_genome/3016810‑3017068
                                                                                                                                      |                                                                                                                            
ttaaaaTAGATTTTGGCAGCTGCAACAGTGCGTTCATATGAACCTTATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATTATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGt                                                                                                                             <  3:243635/134‑1 (MQ=255)
tAAAAATAGATTTTGGCAGCTGCAACAGTGCGTTGATATGAACCTTATCTTTGAAAAACTGTTTCAGCTTGCGGCTTTGGGAGCTCTCAAACAAATGATTCGGAATAATAAAAAATATGTAGCCGCCCGGTTTGGt                                                                                                                             <  4:347204/136‑1 (MQ=255)
tAAAAATAGATTTTGGCAGCTGCAACAGTGCGTTGATATGAACCTTATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATg                                                                                                                          >  7:48689/1‑139 (MQ=255)
tAAAAATAGATTTTGGCAGCGGCAACAGTGCGTTGAAATGAACCTTATCTTTTAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCACTCAAACAAATGATTCGGAAGCATAAAAAAAAAGTAGCCGCCCGGTTTGGt                                                                                                                             <  1:61075/136‑1 (MQ=255)
         aTTTTGGCAGCTGCAACAGTGCGTTGATATGAACCTTATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGct                                                                                                                 <  7:166495/139‑1 (MQ=255)
              ggCAGCTGCAACAGTGCGTTGATATGAACCTTATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGtt                                                                                                            >  5:9225/1‑139 (MQ=255)
                   cgccaccAGTGCTTTCTTATAAACCTTATATTTAAAAACCTGTTTCTCCTGTCCGTTTTGGGAGCTCACAAAAAAATTATTGGCAAAAAAAAAAAATAATAAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATa                                                                                                       <  4:4661/133‑1 (MQ=255)
                             gCGTTGATATGAACCTTATCTTTTAAAAATTGTTTCCGCTTGCCGGTTGGGGAGCTCACAAAAAAATGAATCGCAATAATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAgatga                                                                                              <  8:131221/138‑1 (MQ=255)
                             gCGTTGATATGAACCTTATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAgatgat                                                                                             >  7:79848/1‑139 (MQ=255)
                              cGTTGATATGAACCTTATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGCCGCTCTGTTCAATAAACAGatgatg                                                                                            >  2:143820/1‑139 (MQ=255)
                              cGTTGATATGAACCTTATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAgatgat                                                                                             >  2:323764/1‑138 (MQ=255)
                              cGTTGATATGAACCTTATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAgatgat                                                                                             >  1:89862/1‑138 (MQ=255)
                              cGTTGATATGAACCTTATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAgatgat                                                                                             >  8:124556/1‑138 (MQ=255)
                                 tGATATGAACCTTATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGTTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGc                                                                                         >  3:349289/1‑139 (MQ=255)
                                 tGATATGAACCTTATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGTTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGc                                                                                         >  3:354726/1‑139 (MQ=255)
                                        aaCCTTATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGaa                                                                                  >  2:4820/1‑139 (MQ=255)
                                        aaCCTTATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGaa                                                                                  >  2:49112/1‑139 (MQ=255)
                                        aaCCTTATCTTTGAAAAACTGTTTCAGATTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAATTAGAATACAGGTTTGGTATGCTTGACGATCTGTTCAATAAACAGATGATGAGcagtctc                                                                                   >  5:260490/1‑132 (MQ=255)
                                             tATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGcc                                                                             >  2:99017/1‑139 (MQ=255)
                                             tATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAAAAGATGATGAGCGAAGGAATGGcc                                                                             >  7:267255/1‑139 (MQ=255)
                                             tATCTTTGAAAAACAGTTTCAGAATGCCGCTTTGGGAGCTCTCAAACAAATTATTAGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGAATGCTTGACGCTCTGGTCAATAAACAGATGAAGACCGAAGGAATGGcc                                                                             >  5:173789/1‑139 (MQ=255)
                                              ttCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGGTCTAAAACAAATGATTCGGAATCATAAAAAATAAGTCGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGcc                                                                             <  3:26982/137‑1 (MQ=255)
                                              atatttGAAATACGTTTTCAGATTGCAGGTTTTTGAGATATCAACCTAATGATTTGTAATAATAAAAATTAAGATGCCTCCCGGTTTGGTATGCTTGACGCTCTGTTAAATAAACAGATGATGAGCGAATTAATGGcc                                                                             <  8:237379/135‑1 (MQ=255)
                                              aTCTTTGAAAAACTGTTTCAGCTTGCCGGTTTGGGAGCTCTCAAACAAATGATTCGCAATCATAAAAAATAAGTCGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGcc                                                                             <  8:152373/138‑1 (MQ=255)
                                                   tGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGt                                                                                                                             <  7:259349/85‑1 (MQ=255)
                                                   tGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGt                                                                                                                             >  8:259349/1‑85 (MQ=255)
                                                     aaaaaCTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGCCTTCGTCAg                                                                     >  2:20316/1‑139 (MQ=255)
                                                     aaaaaCTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGGTCAAAAATCAGATTATGAGCGAAGGAATGGCCTGCGTCAg                                                                     >  1:134437/1‑139 (MQ=255)
                                                     aaaaaCTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCAGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGCCTTCGTCat                                                                     >  6:207346/1‑138 (MQ=255)
                                                          cTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGTAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGCCTTCGTCAGCTTTg                                                                <  1:49112/139‑1 (MQ=255)
                                                                acgCTTGCGGCTTTGGGATAGCTCAACCAAAGTATTCGTAATCATAAAAAATACGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGCCTTCGTCAGCTTTGAGCTc                                                           <  8:267255/136‑1 (MQ=255)
                                                                  gCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGCCTTCGTCAGCTTTGAGCTCaaa                                                        <  7:345301/139‑1 (MQ=255)
                                                                     tGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGCCTTCGTCAGCTTTGAGCTCAAACGc                                                     >  3:197628/1‑139 (MQ=255)
                                                                      ggctgtttGGGAGCTCTAAAACAAATGTTTCGTACTCATAAAAATTATGTAGCCGCCCGGTTTGTTATGCGTGACGCTCTGTTCAATAATAAGATGATGAGCGAAGGAATGGCCTTCGTCAGATTTGAGCTCAAACGc                                                     <  2:134437/133‑1 (MQ=255)
                                                                       ccGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGCCTTCGTCAGCTTTGAGCTCAAACGCtt                                                   <  1:99017/139‑1 (MQ=255)
                                                                                       caaaaaacTTATGCGGAATCATAAAAAATAAGAAGCCGCCCGGTTGGTTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGTCCTTCGGCAGCTTTGAGCTCAAACGCTTCTGCTCCTTCGTCAtt                                   <  4:354726/139‑1 (MQ=255)
                                                                                       aaaaaaaaTGATTGGTAAGAAAAAAAAAAAAGTCCCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGCCTTCGTCAGCTTTGAGCTCAAACGCTTCTGCTCCTTCGTCAtt                                   <  6:9225/138‑1 (MQ=255)
                                                                                       aaaaaaaaTGATTCGTAATCAAAAAAAAAAAGTCGCCGCCCGGTTTGTTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGCCTTCGTCAGCTTTGAGCTCAAACGCTTCTGCTCCTTCGTCAtt                                   <  4:349289/138‑1 (MQ=255)
                                                                                              aTGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGCCTTCGTCAGCTTTGAGCTCAAACGCTTCTGCTCCTTCGTCATTCGGataa                            >  7:154151/1‑139 (MQ=255)
                                                                                                      gAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGCCTTCGTCAGCTTTGAGCTCAAACGCTTCTGCTCCTTCGTCATTCGGATAATAGCCGAc                    >  8:97534/1‑139 (MQ=255)
                                                                                                           aTAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGCCTTCGTCAGCTTTGAGCTCAAACGCTTCTGCTCCTTCGTCATTCGGATAATAGCCGACTgg                 >  3:30704/1‑137 (MQ=255)
                                                                                                           aTAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGCCTTCGTCAGCTTTGAGCTCAAACGCTTCTGCTCCTTCGTCATTCGGATAATAGCCGACTGGCa               <  7:97534/139‑1 (MQ=255)
                                                                                                                         gccgccCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGCCTTCGTCAGCTTTGAGCTCAAACGCTTCTGCTCCTTCGTCATTCGGATAATAGCCGACTGGCAGATCACAAATGAc  <  8:48689/138‑1 (MQ=255)
                                                                                                                                      |                                                                                                                            
TAAAAATAGATTTTGGCAGCTGCAACAGTGCGTTGATATGAACCTTATCTTTGAAAAACTGTTTCAGCTTGCCGCTTTGGGAGCTCTCAAACAAATGATTCGGAATCATAAAAAATAAGTAGCCGCCCGGTTTGGTATGCTTGACGCTCTGTTCAATAAACAGATGATGAGCGAAGGAATGGCCTTCGTCAGCTTTGAGCTCAAACGCTTCTGCTCCTTCGTCATTCGGATAATAGCCGACTGGCAGATCACAAATGAC  >  BS168_reference_genome/3016810‑3017068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑