Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	3,882,167	A→T	6.0%	intergenic (‑153/+24)	rocG ← / ← bslB	glutamate dehydrogenase/biofilm protein, member of the processed secretome contributing to biofilm hydrophobicity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	3,882,167	0	A	T	6.0%	210.8 / 3.9	67	intergenic (‑153/+24)	rocG/bslB	glutamate dehydrogenase/biofilm protein, member of the processed secretome contributing to biofilm hydrophobicity
Reads supporting (aligned to +/- strand):  ref base A (29/34);  new base T (2/2);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01

TTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTAATCGTGTAAGTGCCTGCGCGAGGAAGAGTTTTACG  >  BS168_reference_genome/3882034‑3882301                                                                                                                                      |                                                                                                                                       ttttGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGtct                                                                                                                                    <  8:108255/138‑1 (MQ=255)   ttGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTctct                                                                                                                                  <  4:239885/138‑1 (MQ=255)   ttGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCATCATTGGATTCACCCATATCATCAAGCAAGATCCGTACCAGCTTATGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTctct                                                                                                                                  <  5:249695/138‑1 (MQ=255)        cGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTAc                                                                                                                            <  5:977/139‑1 (MQ=255)                     aGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGAt                                                                                                                <  7:139328/138‑1 (MQ=255)                     aGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTGTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGAt                                                                                                                >  6:96589/1‑138 (MQ=255)                           ttAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACggg                                                                                                         >  3:321255/1‑139 (MQ=255)                              aaaacTCAGCCCGCCAATTCCTTTCTTCATTGGATTGACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGat                                                                                                      <  7:304813/139‑1 (MQ=255)                              aaaaaTCAGCCCGCCAATTCCTTTCTTCAATGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGat                                                                                                      <  7:262643/139‑1 (MQ=255)                                      gCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTAACAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCAcc                                                                                              <  1:80431/139‑1 (MQ=255)                                                 ccTTTCTTCATTGGATTCACCCTTATCCTCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGttg                                                                                    <  6:123403/138‑2 (MQ=255)                                                    ttcttcATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAg                                                                                 >  4:307272/1‑138 (MQ=255)                                                      cttcATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCa                                                                               >  8:218781/1‑138 (MQ=255)                                                        tcATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATa                                                                             >  7:45432/1‑138 (MQ=255)                                                            tGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAAc                                                                        >  1:302204/1‑139 (MQ=255)                                                               aTTCCCCCTTATCATCATGGAAGATCCGAACCAGCGTTTGAATCCAAAAAAAACGAGACTGAAAACATCTCAGTCTATGACTACAAAACTATCGATTAAAGGGGAGCAATCACCAGCTGCCTTTAAGCATAAAAACtcc                                                                     >  2:290896/1‑137 (MQ=255)                                                                ttCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGcc                                                                    <  2:324868/139‑1 (MQ=255)                                                                 tCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAGACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGcc                                                                    >  3:264701/1‑138 (MQ=255)                                                                   aCCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACgg                                                                                                          >  3:213195/1‑98 (MQ=255)                                                                   aCCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACgg                                                                                                          <  4:213195/98‑1 (MQ=255)                                                                        tATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGtct                                                                                                                                    <  4:71918/67‑1 (MQ=255)                                                                        tATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGtct                                                                                                                                    >  3:71918/1‑67 (MQ=255)                                                                        tATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGa                                                                  >  3:263012/1‑133 (MQ=255)                                                                        tATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGAATACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGa                                                                  <  4:263012/133‑1 (MQ=255)                                                                          tcatcaTGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCaaa                                                          <  7:218781/139‑1 (MQ=255)                                                                          tcatcaTGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCaaa                                                          <  2:302204/139‑1 (MQ=255)                                                                          tcatcaTGCAAGATCCGTAACAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCaaa                                                          <  1:290896/139‑1 (MQ=255)                                                                           catcatGCAAGATCCGTAGCAGCTTTTGAATACACAAAAAAAGAGACTGAAAACATATCAGTCTCTGAGTACAAATCTATCGATTATCGGGGATCAATCACCAGCTTCGTTTCATCATTAAAACTGCCGATTCCCaaa                                                          <  5:224131/138‑1 (MQ=255)                                                                              catGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCaaa                                                          >  2:35886/1‑135 (MQ=255)                                                                              catGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCaaa                                                          <  1:35886/135‑1 (MQ=255)                                                                               atGCAAGATCTGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGtt                                                     >  4:72711/1‑139 (MQ=255)                                                                               atGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGAt                                                                                                                <  7:6261/80‑1 (MQ=255)                                                                               atGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGAt                                                                                                                >  8:6261/1‑80 (MQ=255)                                                                               atGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGcc                                                                    <  2:70791/124‑1 (MQ=255)                                                                               atGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGcc                                                                    >  1:70791/1‑124 (MQ=255)                                                                               atGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTGCCAAACCGtt                                                     >  6:131597/1‑139 (MQ=255)                                                                               atGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCACACCGtt                                                     >  1:39318/1‑139 (MQ=255)                                                                               atGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGtt                                                     >  5:334862/1‑139 (MQ=255)                                                                               atGCAAGAACCGGACCAGCTTTTGATTCAACAAAAAAAGATACTGAAAACATCACAGTCTCTGAATACAAATATATCGATTAAAGGGGATCAATCACCAGCTGCGTTTCATAAAAAAAACAGCCGATACACAAAccta                                                      >  6:224131/1‑136 (MQ=255)                                                                                tGCAAGATCCGTACCAGATTTTGAATACAAAAAAAAAGATACTGAAAAAATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGTATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGttt                                                    <  2:318584/139‑1 (MQ=255)                                                                                 gCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTta                                                   >  4:90768/1‑139 (MQ=255)                                                                                   aaGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTtac                                                  >  6:17739/1‑138 (MQ=255)                                                                                      aTCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTGCCAAACCGTTTACTACa                                              <  5:131597/139‑1 (MQ=255)                                                                                       tCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAGACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACa                                              <  4:264701/138‑1 (MQ=255)                                                                                        ccGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCaaa                                                          <  2:10987/125‑1 (MQ=255)                                                                                        ccGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCaaa                                                          >  1:10987/1‑125 (MQ=255)                                                                                            aCCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCTGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCt                                        >  1:167365/1‑139 (MQ=255)                                                                                            aCCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCTGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCt                                        >  1:172115/1‑139 (MQ=255)                                                                                                gCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCTGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTaa                                    <  2:172115/139‑1 (MQ=255)                                                                                                gCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCTGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTaa                                    <  2:167365/139‑1 (MQ=255)                                                                                                gCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTaa                                    <  4:321255/139‑1 (MQ=255)                                                                                                 cTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAATACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTaa                                    <  4:181049/138‑1 (MQ=255)                                                                                                 cTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAATACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTaa                                    >  3:181049/1‑138 (MQ=255)                                                                                                 cTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTAAt                                   >  3:208469/1‑139 (MQ=255)                                                                                                 cTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTAAt                                   >  7:12988/1‑139 (MQ=255)                                                                                                     tGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACa                                              <  2:315637/124‑1 (MQ=255)                                                                                                     tGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTCCCGATTCCCAAACCGTTTACTACa                                              >  1:315637/1‑124 (MQ=255)                                                                                                       aaTCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGGTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTAATCGTGTa                             <  3:112161/139‑1 (MQ=255)                                                                                                         tCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTAATc                                  <  8:114823/132‑1 (MQ=255)                                                                                                         tCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTAATc                                  >  7:114823/1‑132 (MQ=255)                                                                                                            acaAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTAATCGTGTAAGTGc                        <  4:208469/139‑1 (MQ=255)                                                                                                              aaaaaaaGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTAATCGTGTAAGTGCCt                      <  5:17739/139‑1 (MQ=255)                                                                                                                        aCTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTAATCGTGTAAGTGCCTGCGCGAGGaa            <  8:12153/139‑1 (MQ=255)                                                                                                                        aCTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTAATCGTGTAAGTGCCTGCGCGAGGaa            <  1:119326/139‑1 (MQ=255)                                                                                                                        aCTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCCTTTACTACATCGCCTTTAATCGTGTAAGTGCCTGCGCGAgg              >  5:348726/1‑137 (MQ=255)                                                                                                                                   ctcAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACa                                              >  6:215310/1‑94 (MQ=255)                                                                                                                                   ctcAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACa                                              <  5:215310/94‑1 (MQ=255)                                                                                                                                      aGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTAATCGTGTAAGTGCCTGCGCGAGGAAGAGTTTTACg  <  7:72325/135‑1 (MQ=255)                                                                                                                                      aGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTAATCGTGTAAGTGCCTGCGCGAGGAAGAGTTTTACg  <  3:72711/135‑1 (MQ=255)                                                                                                                                      aGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTAATCGTGTAAGTGCCTGCGCGAGGAAGAGTTTTACg  <  8:45432/135‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       TTTTGGCCGGCTGTAATGTAAGCGCTTTAAAAAATCAGCCCGCCAATTCCTTTCTTCATTGGATTCACCCTTATCATCATGCAAGATCCGTACCAGCTTTTGAATCCACAAAAAAAGAGACTGAAAACATCTCAGTCTCTGACTACAAATCTATCGATTAACGGGGATCAATCACCAGCTGCGTTTCAGCATAAAAACTGCCGATTCCCAAACCGTTTACTACATCGCCTTTAATCGTGTAAGTGCCTGCGCGAGGAAGAGTTTTACG  >  BS168_reference_genome/3882034‑3882301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑