BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,480,654	Δ1 bp	intergenic (+29/+96)	zwf → / ← gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,480,654	0	T	.	100.0%	524.2 / NA	114	intergenic (+29/+96)	zwf/gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (56/58); total (56/58)

Only 100 of 114 total aligned reads displayed.

CAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATG  >  BS168_reference_genome/2480525‑2480777

caaacaaaACCTTATCCCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTc                                                                                                                   >  1:170180/1‑139 (MQ=255)

             aTCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACCCTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGc                                                                                                     >  7:13840/1‑139 (MQ=255)

             aTCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                      <  5:424371/138‑1 (MQ=255)

             aTCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGc                                                                                                     >  8:43673/1‑139 (MQ=255)

             aTCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGc                                                                                                     >  5:140715/1‑139 (MQ=255)

             aTCTCCTAACTACCAAGCAGGCCCAATGGGAACTAAAGAATCTGATGATCTTTTTGTGAAAGACCGATTACACTGGTGGAACATATAATAAGACGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACga                                                                                                     <  3:291706/139‑2 (MQ=255)

                  cTAACTACGTATCAGTCTCAATGGGACCGAAATAATATGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGAAGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                <  8:446591/139‑1 (MQ=255)

                  cTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                <  4:215667/139‑1 (MQ=255)

                  cTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                <  8:342417/139‑1 (MQ=255)

                  cTAACTACGAATCAGGCGCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                <  7:299966/139‑1 (MQ=255)

                   tAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGCTCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATATTCCG‑CTATATTACGCTGAAc                                                                                                >  4:3486/1‑138 (MQ=255)

                    aaCTACGAATCCGGCTCAATGGTACCGAAAGAATATGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                              <  7:360910/139‑1 (MQ=255)

                    aaCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACa                                                                                               >  4:87896/1‑138 (MQ=255)

                    aaCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCAGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACa                                                                                               >  5:473/1‑138 (MQ=255)

                    aaCTACGAAACAGGCTCAAAGGTCCCGAAAGAAGCTGATGAGATTTTGGTTAAACACGGCTTACACTGGTGTAACATAAAATAAGAAGAAAAAAGCCTAAAATGTTTAC‑AAGCATTTTAGG‑CTTTTTTACGCTGAACaa                                                                                              <  8:170574/139‑1 (MQ=255)

                     aCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACa                                                                                               <  5:122229/137‑1 (MQ=255)

                     aCTACGAATAAGGCTCAATGGGACCGAAAGAATCTTATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                              <  4:283124/138‑1 (MQ=255)

                       tACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAgg                                                                                           <  8:380636/139‑1 (MQ=255)

                         cGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGCAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGta                                                                                         <  7:41314/139‑1 (MQ=255)

                            aTCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                              <  8:287278/131‑1 (MQ=255)

                            aTCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                              >  7:287278/1‑131 (MQ=255)

                            aTCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATaa                                                                                      >  8:155710/1‑139 (MQ=255)

                            aTCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATaa                                                                                      >  3:106184/1‑139 (MQ=255)

                            aTCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATaa                                                                                      >  3:439350/1‑139 (MQ=255)

                               aGGATCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACaa                                                                                   >  3:189335/1‑139 (MQ=255)

                                 gctgattGGGACCGTAAGAATCTGATGATGTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                 <  6:337598/133‑1 (MQ=255)

                                 gCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGTTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                 <  2:4355/139‑1 (MQ=255)

                                 gCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGTTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                 <  2:11585/139‑1 (MQ=255)

                                 gCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                >  7:270617/1‑124 (MQ=255)

                                 gCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                <  8:270617/124‑1 (MQ=255)

                                 gCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATATTCGG‑CTTTTTTACTCTGAACAAAGGAATAACAAtc                                                                                 >  7:160458/1‑139 (MQ=255)

                                 gCTCAATCGGACCGAAATAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                  >  8:122994/1‑138 (MQ=255)

                                           aCCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTtaataa                                                                       >  1:425908/1‑139 (MQ=255)

                                           aCCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTtaataa                                                                       >  5:345665/1‑139 (MQ=255)

                                           aCCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTtaataa                                                                       >  7:369191/1‑139 (MQ=255)

                                               aaaGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAg                                                                                            <  3:129844/114‑1 (MQ=255)

                                               aaaGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAg                                                                                            >  4:129844/1‑114 (MQ=255)

                                               aaaGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaaa                                                                   <  1:420148/139‑1 (MQ=255)

                                                     tCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaa                                                                    <  1:401106/132‑1 (MQ=255)

                                                     tCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaa                                                                    >  2:401106/1‑132 (MQ=255)

                                                     tCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAcccc                                                              >  7:35592/1‑138 (MQ=255)

                                                     tCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAcccc                                                              >  8:381887/1‑138 (MQ=255)

                                                       tgatgaTCTTTTGGTGAAAGAAGGCTTACAATGGTGGAACATATAATAAGAAGAAAACAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                            <  3:118871/138‑1 (MQ=255)

                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAAGAATCTCCTTAATAACAAAACCCCCa                                                            <  7:450211/136‑1 (MQ=255)

                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                            <  3:215204/136‑1 (MQ=255)

                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                            <  8:35592/136‑1 (MQ=255)

                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                            <  5:324707/136‑1 (MQ=255)

                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                            <  3:87896/136‑1 (MQ=255)

                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAg                                                         >  3:315193/1‑139 (MQ=255)

                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAg                                                         >  8:287777/1‑139 (MQ=255)

                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAg                                                         >  2:381053/1‑139 (MQ=255)

                                                              cTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACa                                                                                    >  5:226154/1‑107 (MQ=255)

                                                              cTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACa                                                                                    <  6:226154/107‑1 (MQ=255)

                                                                tttGGTGAAAGACGGCTTACACTGGTTGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAag                                                   <  6:140715/138‑1 (MQ=255)

                                                                 ttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAagag                                                 >  4:208436/1‑139 (MQ=255)

                                                                 ttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAag                                                   <  3:326893/137‑1 (MQ=255)

                                                                 ttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAag                                                   <  7:122994/137‑1 (MQ=255)

                                                                 ttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAag                                                   <  1:381053/137‑1 (MQ=255)

                                                                 ttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAACCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGAATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAag                                                   <  7:405603/137‑1 (MQ=255)

                                                                         aGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                            <  5:247793/120‑1 (MQ=255)

                                                                         aGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                            >  6:247793/1‑120 (MQ=255)

                                                                             ggCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGttt                                     <  3:208436/139‑1 (MQ=255)

                                                                              gCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTc                                    >  6:300781/1‑139 (MQ=255)

                                                                              gCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTc                                    >  5:236043/1‑139 (MQ=255)

                                                                               cTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTc                                    >  7:15928/1‑138 (MQ=255)

                                                                                    acTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGa                              <  7:287777/139‑1 (MQ=255)

                                                                                       ggtggCACATATAAGAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGACCAAAGGTAGAACAATCTCCTTAATAACAAAACCCCCAACGCTTAAGAGCGTCGGGGGTTTCTATTGATTa                           >  7:377585/1‑139 (MQ=255)

                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCTAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAAAAATCTCCTTAATAACAAAACCCACAAAGCTTAAGAGATTCGGGGTTTTCTATTGATTAc                          >  1:210115/1‑139 (MQ=255)

                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                              >  6:117621/1‑71 (MQ=255)

                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTa                                  >  3:122759/1‑131 (MQ=255)

                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTa                                  <  4:122759/131‑1 (MQ=255)

                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                          >  3:101513/1‑139 (MQ=255)

                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                          >  4:28172/1‑139 (MQ=255)

                                                                                         tggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                              <  7:164945/70‑1 (MQ=255)

                                                                                         tggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                              >  8:164945/1‑70 (MQ=255)

                                                                                                tattatAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGt                                       <  1:152357/118‑1 (MQ=255)

                                                                                                tataATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGt                                       >  2:152357/1‑118 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATATCAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCa                      >  5:403313/1‑133 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACa                                                                                    <  3:185267/71‑1 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACa                                                                                    >  4:185267/1‑71 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                          <  7:195818/129‑1 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                          >  8:195818/1‑129 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCa                      <  6:403313/133‑1 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                <  2:87850/139‑1 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                >  4:88714/1‑139 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                <  1:416723/139‑1 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                >  2:311513/1‑139 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAt                 >  5:29159/1‑138 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTGTAC‑AATCATTTTCGG‑CTTTTTTACGCTGAACAACGGTATAACAATCTCCTTAATTACCAAACCCCCAAAGCTTCAGAGCGTCCGGCGTTTCTATTGATTTCTTCATCCag                 >  5:298/1‑137 (MQ=255)

                                                                                                   aataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                >  5:64598/1‑138 (MQ=255)

                                                                                                      aagaagAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAg             >  6:314629/1‑138 (MQ=255)

                                                                                                       agaagaAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTa           <  4:439350/139‑1 (MQ=255)

                                                                                                       agaagaAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTACATAATAACAAAAACCCCAAAGCTTAAGAGCTTCGGGGGTTTCTAATGATTACTTCATCCATTCAGTa           <  8:196161/139‑1 (MQ=255)

                                                                                                        gaagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTAt          >  2:347316/1‑139 (MQ=255)

                                                                                                          agaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                <  4:417133/131‑1 (MQ=255)

                                                                                                          agaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATg         >  6:200506/1‑138 (MQ=255)

                                                                                                           gaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGa       <  2:372822/139‑1 (MQ=255)

                                                                                                            aaaaaaGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGaa      <  7:381887/139‑1 (MQ=255)

                                                                                                              aaaaGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCTCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGa    <  8:355547/139‑1 (MQ=255)

                                                                                                                aaGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATg  <  7:140077/139‑1 (MQ=255)

CAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATG  >  BS168_reference_genome/2480525‑2480777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`