Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	3,059,489	(C)8→7	intergenic (‑88/+58)	ytnP ← / ← trmB	putative quorum‑quenching lactonase/tRNA (guanine‑N(7)‑)‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	3,059,482	0	C	.	100.0%	259.9 / NA	58	intergenic (‑81/+65)	ytnP/trmB	putative quorum‑quenching lactonase/tRNA (guanine‑N(7)‑)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (29/29);  total (29/29)

TTTTTCATTGACTGGATATTTTTTAGA‑CCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCACCCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTGGCCGAGTGCAGAGAATTTTTCTTCATATTC  >  BS168_reference_genome/3059345‑3059615                                                                                                                                           |                                                                                                                                       tttttCATTGACTGGATATTTTTTAGA‑CCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAc                                                                                                                                        <  5:247011/138‑1 (MQ=255) tttttCATTGACTGGATATTTTTTAGA‑CCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAc                                                                                                                                        <  3:364490/138‑1 (MQ=255)   tttCATTGACTGGATATTTTTTAGA‑CCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAccc                                                                                                                                      <  2:123996/138‑1 (MQ=255)      cATTGACTGGATATTTTTTAGC‑CCAAAGCTGATTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGGGTCACGCCTCCTTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAcccccc                                                                                                                                   <  1:131969/138‑1 (MQ=255)      cATTGACTGGATATTTTTTAGA‑GCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAcccccc                                                                                                                                   <  1:72996/138‑1 (MQ=255)      cATTGACTGGATATTTTTTAGA‑CCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAcccccc                                                                                                                                   <  2:373114/138‑1 (MQ=255)           aCTGGATATTTTTTAGA‑CCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAccccccctatc                                                                                                                              <  2:153102/138‑5 (MQ=255)           aCTGGATATTTTTTAGA‑CCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCA‑TTTCA‑CCCCCCCTatcat                                                                                                                           <  3:121442/139‑1 (MQ=255)               gctatTTTTTAGACCCAACGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTAtcatca                                                                                                                         <  1:332871/137‑1 (MQ=255)               gATATTTTTTAGA‑CCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCACCGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATcatcat                                                                                                                        >  6:284602/1‑139 (MQ=255)                   ttttttAGA‑CAACAGGGGATTTGGCACAACGCCGAAAATGGCGCCTCCTTCCTTATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGt                                                                                                                     <  3:258973/138‑1 (MQ=255)                          gA‑CCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAAc                                                                                                              <  8:5664/138‑1 (MQ=255)                                aaGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGccc                                                                                                         >  2:199256/1‑138 (MQ=255)                                      cTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATATTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑aaaa                                                                                                  >  6:283900/1‑138 (MQ=255)                                           gCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCCCGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACa                                                                                            >  2:33941/1‑139 (MQ=255)                                           gCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACa                                                                                            >  2:40962/1‑139 (MQ=255)                                              caACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGAc                                                                                         <  7:221709/139‑1 (MQ=255)                                                aCGCCGAACATTGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATCGTAGCAAACGGCACAA‑AAAAAAAACAGACTc                                                                                       >  8:368188/1‑139 (MQ=255)                                                aCGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCGAACGGCCCAT‑AAAAAAAACAGACTc                                                                                       >  1:120459/1‑139 (MQ=255)                                                aCGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGtctc                                                                                       <  7:20788/139‑1 (MQ=255)                                                aCGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTc                                                                                       >  8:366096/1‑139 (MQ=255)                                                aCGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTc                                                                                       <  8:306681/139‑1 (MQ=255)                                                aCGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTc                                                                                       >  1:217090/1‑139 (MQ=255)                                                    cGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGt                                                                                   <  3:33788/139‑1 (MQ=255)                                                        cATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCaga                                                                               <  2:120459/139‑1 (MQ=255)                                                         aTGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCagag                                                                              <  1:199256/139‑1 (MQ=255)                                                          tGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAgt                                                                             <  3:287802/139‑1 (MQ=255)                                                          tGGCGCCTCCGTCCATATGCGTCACGCATGCGTCAAGCCAGGTCAACGTGATGTTTCCAATTATAATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAgt                                                                             <  5:125057/139‑1 (MQ=255)                                                           ggCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCATGTCAACGTGATGTTTCCAATTTTCATTGTTTACATTTTCA‑CCCACCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACACATCTCagagac                                                                            >  6:196211/1‑137 (MQ=255)                                                                          atGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTtctc                                                             >  3:457523/1‑139 (MQ=255)                                                                          atGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTtctc                                                             >  1:17778/1‑139 (MQ=255)                                                                           tGCGTCACGCCGACGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTtctc                                                             <  3:305599/138‑1 (MQ=255)                                                                             cGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACCGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCAt                                                          >  2:404659/1‑139 (MQ=255)                                                                               tCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAccccccctat                                                                                                                               <  6:339899/70‑4 (MQ=255)                                                                               tCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAccccccctat                                                                                                                               >  5:339899/1‑67 (MQ=255)                                                                               tCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATCGTAGCAAACGGCCCAT‑AAAAAAAACAGAATCCTGTCCGAGTCTGTATTAACGGTCTCCaat                                                         >  6:96567/1‑136 (MQ=255)                                                                               tCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑‑CCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCAtt                                                         >  6:206358/1‑138 (MQ=255)                                                                               tCACGCCTCCGTCAAGCCAGGTCAACGTGATCTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATAATGTTAGCAAACGCCCCAT‑AAAAAAAACAGACTCCTGTCAGATTCTGAAGATACGTTCTCAAtt                                                         >  4:337249/1‑138 (MQ=255)                                                                               tCACGCCTCCGTCAAGCCACGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCAtt                                                         >  3:386350/1‑138 (MQ=255)                                                                                    ccTGCGTCAAGCCAGGTCAACGTGATGTTTCGAATTTTCATTGTTTCCATTTTCA‑GCCCCCCTATAATCATGGTTGAAAACGGCACAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACTTTCTCCATTCAAGCt                                                   <  3:337249/139‑1 (MQ=255)                                                                                    ccTCCGTCAAGCCATGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCt                                                   <  7:368188/139‑1 (MQ=255)                                                                                    ccTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCt                                                   <  7:51312/139‑1 (MQ=255)                                                                                        cggcAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCa                                                 <  6:299464/134‑1 (MQ=255)                                                                                        cGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGc                                               >  7:453187/1‑139 (MQ=255)                                                                                          tCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGccc                                             >  5:417498/1‑139 (MQ=255)                                                                                            aaGCCTGGTCAACGTGATGTTTCCATTTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCAGCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGa                                           <  4:333912/139‑1 (MQ=255)                                                                                              gCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATa                                         <  4:457523/139‑1 (MQ=255)                                                                                               ccAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTAGGTTCTCCATTCAACCTCAGCCCGATAg                                        >  4:263481/1‑139 (MQ=255)                                                                                               ccAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGa                                           >  2:90948/1‑136 (MQ=255)                                                                                               ccAGGTCAACGTGATGTTTCCAAATTTCAATGTTTACATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCAAA‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTACTCCAATCAACCTCAGCCCGa                                           >  1:152807/1‑136 (MQ=255)                                                                                                cAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGa                                       <  7:366096/139‑1 (MQ=255)                                                                                                cAGGTAAACGTGATGTTTCCAATTTTAATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGa                                       <  4:256458/139‑1 (MQ=255)                                                                                                  ggTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCAtt                                                         >  2:222288/1‑119 (MQ=255)                                                                                                  ggTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCAtt                                                         <  1:222288/119‑1 (MQ=255)                                                                                                   gTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGAc                                                                                         >  7:330463/1‑86 (MQ=255)                                                                                                   gTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGAc                                                                                         <  8:330463/86‑1 (MQ=255)                                                                                                   gTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCttt                                                                     >  1:364569/1‑106 (MQ=255)                                                                                                   gTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCttt                                                                     <  2:364569/106‑1 (MQ=255)                                                                                                   gTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGa                                           >  7:344816/1‑132 (MQ=255)                                                                                                        cGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTgg                               >  5:135814/1‑139 (MQ=255)                                                                                                          tGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCGCTATCATCCTTGTAGCAACCGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCACGCTAGATCGGCTGGcc                             <  1:60551/139‑1 (MQ=255)                                                                                                            aTGTTTCAAAATTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTATCCATTCAACCTCAGCCCGATAGATCGGCTGGcc                             >  8:152372/1‑137 (MQ=255)                                                                                                                    aaTTTTCATTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTGGCCGAGTGCagag                   <  6:135814/139‑1 (MQ=255)                                                                                                                           aTTGTTTCCATTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTGGCCGAGTGCAGAGAAttttt            >  3:193091/1‑139 (MQ=255)                                                                                                                                    aTTTTCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTGGCCGAGTGCAGAGAATTTTTCTTCATAtt   <  2:125201/139‑1 (MQ=255)                                                                                                                                     ttttCA‑CCCCCCCTATCATCATGGTAGCATACGGCCCAT‑AAAAAAAACAGACTCCTGGCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTGGCCGAGTGCAGAGAATTTTTCTTCATATTc  <  5:196211/139‑1 (MQ=255)                                                                                                                                     ttttCA‑CCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTGGCCGAGTGCAGAGAATTTTTCTTCATATTc  <  1:452679/139‑1 (MQ=255)                                                                                                                                           |                                                                                                                                       TTTTTCATTGACTGGATATTTTTTAGA‑CCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCACCCCCCCCTATCATCATGGTAGCAAACGGCCCAT‑AAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTGGCCGAGTGCAGAGAATTTTTCTTCATATTC  >  BS168_reference_genome/3059345‑3059615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑