Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	166,345	Δ1 bp	intergenic (+17/‑155)	trnI‑Ala → / → rrnH‑16S	tRNA‑Ala/ribosomal RNA‑16S

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	166,344	0	G	.	100.0%	155.8 / NA	82	intergenic (+16/‑156)	trnI‑Ala/rrnH‑16S	tRNA‑Ala/ribosomal RNA‑16S
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (43/39);  total (43/39)

TGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAGGTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAA  >  BS168_reference_genome/166210‑166466                                                                                                                                       |                                                                                                                           tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGCCCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCCCCAAAAGTTTTTAAAAAAgttat                                                                                                                        >  3:401104/1‑135 (MQ=12) tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGCCCATGTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCGCGCAGGAGGTCCTCGGTTCGCTCCCGCTAGGCTCCACCAAACGTTTTTAAAAAAgttat                                                                                                                        >  1:375040/1‑135 (MQ=11) tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCCATCTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat                                                                                                                        >  4:158748/1‑135 (MQ=255) tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTATGCTCCACCAAAAGTTTTTTAAAAAgttat                                                                                                                        >  6:99197/1‑135 (MQ=12) tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat                                                                                                                        >  4:412595/1‑135 (MQ=17) tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat                                                                                                                        >  3:334663/1‑135 (MQ=17) tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat                                                                                                                        >  7:44948/1‑135 (MQ=17) tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat                                                                                                                        >  4:135942/1‑135 (MQ=17) tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat                                                                                                                        <  7:331020/139‑5 (MQ=17) tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat                                                                                                                        >  2:10663/1‑135 (MQ=17) tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat                                                                                                                        >  7:300087/1‑135 (MQ=17) tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat                                                                                                                        >  6:190893/1‑135 (MQ=17)      tCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCCCGCAGGAGGTCAGCGGTTCGGTCCCGCTAGGCGCCCCCAAAAGTTTTTAAAAAA‑GTTATTGACt                                                                                                                  >  4:266649/1‑139 (MQ=11)       cGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACtt                                                                                                                 >  5:220179/1‑139 (MQ=17)       cGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACtt                                                                                                                 >  3:432224/1‑139 (MQ=17)        gCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACttt                                                                                                                <  3:135942/139‑1 (MQ=17)         cAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACt                                                                                                                  <  5:303980/136‑1 (MQ=17)         cAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACt                                                                                                                  >  6:303980/1‑136 (MQ=17)          aGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTga                                                                                                              <  4:77155/139‑1 (MQ=17)               cGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGATCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTgaagaa                                                                                                          <  4:432224/138‑1 (MQ=12)               cGAATCCTGTCGTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCCCGCAGGAGGTCAGCGGTTCGATCCCGCAAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGaagaag                                                                                                         >  3:105988/1‑139 (MQ=11)                gcaTCCTGGCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGaagaag                                                                                                         <  4:105988/136‑1 (MQ=12)                 aaTCCTGTCTTGCAGACCATTTTTTATGGGGACTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTg                                                                                                       <  3:458896/139‑1 (MQ=11)                 aaTCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTg                                                                                                       <  4:218808/139‑1 (MQ=17)                 aaTCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTg                                                                                                       <  1:456635/139‑1 (MQ=17)                 aaTCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTg                                                                                                       <  7:150375/139‑1 (MQ=17)                 aaTCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTg                                                                                                       <  3:80532/139‑1 (MQ=17)                 aaTACTGTCTTCCCGAACATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTg                                                                                                       <  8:403622/139‑1 (MQ=255)                  aTCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGa                                                                                                      <  1:269039/139‑1 (MQ=17)                        tCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCCCCAAAACTTTTTAAAAAA‑TTTATTTACTTTGAAGAAGTGCCATTg                                                                                                 >  1:228735/1‑138 (MQ=11)                        tCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTg                                                                                                 >  4:47467/1‑138 (MQ=21)                            cccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGtat                                                                                              <  4:235565/137‑1 (MQ=21)                            cccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGta                                                                                               <  3:445470/136‑1 (MQ=21)                            cccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGta                                                                                               <  7:52050/136‑1 (MQ=21)                            cccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGta                                                                                               <  2:15523/136‑1 (MQ=21)                            cccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATAc                                                                                            >  8:104599/1‑139 (MQ=21)                            cccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGGTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGtat                                                                                              <  8:300087/137‑1 (MQ=255)                               gACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACtaa                                                                                         <  6:220179/139‑1 (MQ=21)                               gACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACtaa                                                                                         >  3:154932/1‑139 (MQ=21)                               gACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACtaa                                                                                         >  3:164590/1‑139 (MQ=21)                                      ttttATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGt                                                                                   <  4:334663/138‑1 (MQ=11)                                           tGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCttt                                                                             <  8:385242/139‑1 (MQ=11)                                                    aGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAg                                                                      <  8:4794/137‑1 (MQ=11)                                                    aGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCg                                                                    >  4:33160/1‑139 (MQ=11)                                                     gCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCg                                                                    <  3:158748/138‑1 (MQ=11)                                                         aGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGaca                                                                <  4:308748/138‑1 (MQ=11)                                                          gCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTAATGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGacaaa                                                              >  3:113224/1‑139 (MQ=2)                                                           cTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTATTAAAGTTGCTTTACCAACGCGGacaaa                                                              >  2:194569/1‑138 (MQ=11)                                                           cTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCt                                                                               <  6:17558/121‑1 (MQ=11)                                                           cTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCt                                                                               >  5:17558/1‑121 (MQ=11)                                                                   gcgcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAAt                                                        <  6:177750/136‑1 (MQ=11)                                                                   gcgcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAAt                                                        >  5:177750/1‑136 (MQ=11)                                                                   gcgcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGAt                                                     >  4:155753/1‑139 (MQ=11)                                                                   gcgcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGAt                                                     >  4:122512/1‑139 (MQ=11)                                                                    cgcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATc                                                    >  5:40090/1‑139 (MQ=11)                                                                     gcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGCCTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCt                                                   >  6:210198/1‑139 (MQ=2)                                                                     gcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAACCAAAATGATCt                                                   >  3:74106/1‑139 (MQ=2)                                                                             tGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGaaaa                                            <  8:403760/138‑1 (MQ=11)                                                                                 cGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTaaa                                       <  4:154932/139‑1 (MQ=11)                                                                                 cGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTaaa                                       <  4:164590/139‑1 (MQ=11)                                                                                    aggaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCttt                                                 <  1:427479/126‑1 (MQ=11)                                                                                    aggaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCttt                                                 >  2:427479/1‑126 (MQ=11)                                                                                    aggaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAAc                                      >  1:420707/1‑137 (MQ=11)                                                                                    aggaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAAc                                      >  1:271792/1‑137 (MQ=11)                                                                                     ggaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAAc                                      >  6:353849/1‑136 (MQ=11)                                                                                      gaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGa                                  <  7:130023/139‑1 (MQ=11)                                                                                      gaggTCAGCGGGTCGATCCCGCTAGGCTCCACAAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGa                                  <  1:13626/139‑1 (MQ=2)                                                                                         gTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACa                                >  3:109045/1‑138 (MQ=11)                                                                                            aGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAAc                                      <  7:315335/129‑1 (MQ=11)                                                                                            aGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAAc                                      >  8:315335/1‑129 (MQ=11)                                                                                            aGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAAc                            >  5:37642/1‑139 (MQ=11)                                                                                             gCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACg                           <  7:150763/139‑1 (MQ=11)                                                                                             gCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACg                           <  5:190893/139‑1 (MQ=11)                                                                                                 ttCGATCCCGCTAGGCTCCACCAAAATTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTAcc                       <  2:84250/139‑1 (MQ=2)                                                                                                   cGATCCCGCTAGGCTCCCCCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAATCTAAACAAGACAAAACGTACCTg                     >  8:124083/1‑139 (MQ=2)                                                                                                   cGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTg                     >  8:93430/1‑139 (MQ=11)                                                                                                   cGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTg                     >  8:53528/1‑139 (MQ=11)                                                                                                    gATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTACCAAAGCGGACAAACAAAATGATCTTTGAACACTAAACACGCCGAAGCGTACCTgg                    >  3:295384/1‑138 (MQ=11)                                                                                                           cTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAAtt               >  1:436404/1‑137 (MQ=11)                                                                                                           cTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAAtt               >  8:294287/1‑137 (MQ=11)                                                                                                               gCTCCACCAAAAGTTTTTAAATAA‑GTTATTGACTTTGAAGAAGTCACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCACttt         >  6:153352/1‑139 (MQ=11)                                                                                                                cTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGtttt        >  2:417194/1‑139 (MQ=11)                                                                                                                   caccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGaaaa                                            <  1:108466/100‑1 (MQ=9)                                                                                                                   caccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAAc                                      <  7:430965/106‑1 (MQ=11)                                                                                                                   caccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAAc                                      >  8:430965/1‑106 (MQ=11)                                                                                                                   caccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTa      >  8:200060/1‑138 (MQ=11)                                                                                                                   caccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTa      >  8:355355/1‑138 (MQ=11)                                                                                                                   caccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTa      >  8:387883/1‑138 (MQ=11)                                                                                                                   caccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTa      >  8:51571/1‑138 (MQ=11)                                                                                                                   caccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAATTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGaaaa                                            >  2:108466/1‑100 (MQ=9)                                                                                                                   caccaAAAGTTTTTAAAA‑‑‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTaaa    >  6:242761/1‑138 (MQ=255)                                                                                                                   aaccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTa      >  4:136338/2‑138 (MQ=11)                                                                                                                      caAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTaaaaa  <  3:47467/139‑1 (MQ=11)                                                                                                                      caAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTaaaaa  <  5:66357/139‑1 (MQ=11)                                                                                                                                       |                                                                                                                           TGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAGGTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAA  >  BS168_reference_genome/166210‑166466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑