BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,271,424	T→C	R78R (AGA→AGG)	uvrX ←	lesion bypass phage DNA polymerase; phage SPbeta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,271,424	0	T	C	100.0%	333.5 / NA	118	R78R (AGA→AGG)	uvrX	lesion bypass phage DNA polymerase; phage SPbeta
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (53/65); total (53/65)

Only 100 of 118 total aligned reads displayed.

TTCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATTCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAACACTACACTTCCCTGTCTATCCGTATTCCCTACAACAGCAAGATAGCATGTTAA  >  BS168_reference_genome/2271291‑2271558

ttCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCtt                                                                                                                                     <  1:296964/137‑1 (MQ=255)

ttCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCtt                                                                                                                                     <  8:140400/137‑1 (MQ=255)

 tCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTgg                                                                                                                                   >  3:371499/1‑138 (MQ=255)

  catGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACTTTTCTCAGGAAAAAATCTGTAAACCAGCTTTGTATTTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGAt                                                                                                                                 >  8:16762/3‑139 (MQ=14)

     gCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCt                                                                                                                               >  7:172979/1‑138 (MQ=255)

            aaaaaGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAgg                                                                                                                          <  2:263003/136‑1 (MQ=255)

            aaaaaGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAg                                                                                                                           <  4:88793/135‑1 (MQ=255)

            aaaaaGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAg                                                                                                                           <  1:440314/135‑1 (MQ=255)

            aaaaaGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAg                                                                                                                           <  8:7808/135‑1 (MQ=255)

            aaaaaGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGt                                                                                                                         <  3:240496/137‑1 (MQ=255)

                  aTTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCaaa                                                                                                                 >  2:96798/1‑139 (MQ=255)

                   ttCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCaaa                                                                                                                 >  3:283452/1‑138 (MQ=255)

                           ttGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGa                                                                                                        >  6:95994/1‑139 (MQ=255)

                                     aTGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGtttt                                                                                               >  5:395736/1‑138 (MQ=38)

                                     aTGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGtttt                                                                                               >  2:386381/1‑138 (MQ=38)

                                        gACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGAtt                                                                                           >  5:113318/1‑139 (MQ=37)

                                        gACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGAt                                                                                            >  2:122758/1‑138 (MQ=37)

                                            caTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                       <  1:122758/139‑1 (MQ=37)

                                            caTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCa                                                                                        >  2:327836/1‑138 (MQ=37)

                                             aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                       >  6:100595/1‑138 (MQ=37)

                                             aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                       >  7:205504/1‑138 (MQ=37)

                                             aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                       >  7:413753/1‑138 (MQ=37)

                                             aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                       >  2:197348/1‑138 (MQ=37)

                                             aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                       >  3:114927/1‑138 (MQ=37)

                                             aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                       >  7:108286/1‑138 (MQ=37)

                                             aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                       >  3:224433/1‑138 (MQ=37)

                                             aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                       >  5:407245/1‑138 (MQ=37)

                                             aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                       >  3:278757/1‑138 (MQ=37)

                                             aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCATGTTTTGATTCCaa                                                                                       >  2:50998/1‑138 (MQ=255)

                                             aTTTCTCAGGAACAAATCTGTAAAACAGCTATGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCTATCCTGTTTTGATTCCaa                                                                                       >  2:87663/1‑138 (MQ=255)

                                              tttCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaaaa                                                                                     <  1:96798/139‑1 (MQ=37)

                                               ttCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAg                                                                                                                           >  5:42763/1‑100 (MQ=255)

                                               ttCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAg                                                                                                                           <  6:42763/100‑1 (MQ=255)

                                                      gAACAAATCTGTAAAAGATCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCATCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCtttttt                                                                             <  5:9694/139‑1 (MQ=255)

                                                      gAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCtttttt                                                                             <  3:31078/139‑1 (MQ=21)

                                                      gAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCtttttt                                                                             <  2:31224/139‑1 (MQ=21)

                                                      gAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCtttttt                                                                             <  1:304657/139‑1 (MQ=21)

                                                      gAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCtttttt                                                                             <  7:298100/139‑1 (MQ=21)

                                                       aaCAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCtttttt                                                                             >  6:426662/1‑138 (MQ=21)

                                                       aaCAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCtttttt                                                                             >  5:210361/1‑138 (MQ=21)

                                                       aaCAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATAAGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGACTCGATCCTGTTTTGATACCAAAATCttattt                                                                             >  4:389027/1‑138 (MQ=11)

                                                        aCAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTaa                                                                           <  4:371499/139‑1 (MQ=21)

                                                           aaTCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACATTGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTATGATTCCACACTCTTTTTTAAGTg                                                                        >  8:307601/1‑139 (MQ=11)

                                                            aTCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTg                                                                        >  1:257328/1‑138 (MQ=21)

                                                            aTCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGc                                                                       <  8:452886/139‑1 (MQ=21)

                                                            aTCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGc                                                                       <  6:313029/139‑1 (MQ=21)

                                                                 tAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTAt                                                                                                                       >  1:323051/1‑86 (MQ=255)

                                                                 tAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTAt                                                                                                                       <  2:323051/86‑1 (MQ=255)

                                                                 tAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAg                                                                     >  1:156910/1‑136 (MQ=21)

                                                                   aaaaAGCTTTGTAATTTCAGTAGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt                                                                <  3:389898/139‑1 (MQ=255)

                                                                   aaaCATCTTTGTAATTTCAGTGGAAACTCTGATGTAAAGCTTCATTTGTGGATATACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCTAAATCTTTTTTAAGTGCAGGAGAt                                                                <  7:94269/139‑1 (MQ=11)

                                                                   aaaCAGCTTTGTAATTTCCGTTGAAACTCTGATGAAAAGCTGCATTTGGGGCTTTACAATGTGGATCCTTGGAGCGTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAAGCTTTTTTAAGTGCAGGAGAt                                                                <  2:309514/139‑1 (MQ=11)

                                                                   aaaCAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt                                                                <  4:266321/139‑1 (MQ=21)

                                                                   aaaCAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt                                                                <  2:77300/139‑1 (MQ=21)

                                                                        gCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                           <  6:381257/139‑1 (MQ=21)

                                                                        gCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                           <  6:187067/139‑1 (MQ=21)

                                                                         cTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                           >  6:101487/1‑138 (MQ=21)

                                                                         cTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                           >  4:219717/1‑138 (MQ=21)

                                                                         cTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                           <  4:197103/138‑1 (MQ=21)

                                                                           ttGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCATGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGAAGCTa                                                         >  6:447314/1‑138 (MQ=255)

                                                                            tGTAATTTCAGTTGTAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATATCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                           <  6:340278/135‑1 (MQ=255)

                                                                              tAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTaata                                                      <  5:115938/138‑3 (MQ=21)

                                                                                   tCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTaa                                                        >  6:134224/1‑131 (MQ=17)

                                                                                   tCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTaa                                                        <  5:134224/131‑1 (MQ=17)

                                                                                   tCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAatactaca                                                 <  4:278757/138‑1 (MQ=17)

                                                                                    cAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTATGTGCAGGAGATGCAGCTAatactacac                                                <  5:101487/138‑1 (MQ=12)

                                                                                       ttGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTcc                                            <  5:95994/139‑1 (MQ=17)

                                                                                            aCTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGTTCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTc                                       <  4:114927/139‑1 (MQ=12)

                                                                                            aCTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGCATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTc                                       <  7:16762/139‑1 (MQ=12)

                                                                                            aCTCTGATGAAAAGCTTCATTTGTGGATTTACAATGGGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTc                                       <  7:289605/139‑1 (MQ=12)

                                                                                             ctctGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCt                                      <  1:386381/139‑1 (MQ=17)

                                                                                                tgatgaAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCtc                                     <  1:428060/137‑2 (MQ=12)

                                                                                                 gatgaAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                           >  3:320569/1‑114 (MQ=17)

                                                                                                 gatgaAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                           <  4:320569/114‑1 (MQ=17)

                                                                                                  atgaACAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGAGCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCtct                                    <  4:385357/136‑3 (MQ=11)

                                                                                                  atgaAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCtct                                    <  5:364985/136‑3 (MQ=12)

                                                                                                  atgaAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTcc                                  <  2:257328/138‑1 (MQ=12)

                                                                                                     aacaGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGGTTTGATTCCAAAATCTTTTTTAAGGGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa                               <  3:24794/135‑1 (MQ=11)

                                                                                                     aaaaGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa                               <  2:156910/138‑1 (MQ=12)

                                                                                                     aaaaGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa                               <  6:173443/138‑1 (MQ=12)

                                                                                                            tCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAatactaca                                                 <  8:66347/113‑1 (MQ=12)

                                                                                                            tCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAatactaca                                                 >  7:66347/1‑113 (MQ=12)

                                                                                                             cATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTac                       <  8:413753/138‑1 (MQ=12)

                                                                                                             cATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTac                       <  4:224433/138‑1 (MQ=12)

                                                                                                                     gATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTac                       <  3:184004/130‑1 (MQ=12)

                                                                                                                     gATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTac                       >  4:184004/1‑130 (MQ=12)

                                                                                                                        ttACAATGTGTATCCTTGGATCTTCAGGTTTCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATGTTTTTTAAGTGCTGGAGATGCAGCTAATTCTACACTTCCCTGGCTCTCCGTATTCCCTACAACAGCAAGa             >  7:440731/1‑137 (MQ=11)

                                                                                                                          aCAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAg          <  5:100595/138‑1 (MQ=255)

                                                                                                                           ctaTGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGTTCCTGTTTTTATTCCAAAATCTTTTTTAAGTGCCGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc         <  5:447314/136‑1 (MQ=11)

                                                                                                                           ctaTGTGTATCCTTGGATATTCAGGTATATCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTTCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc         <  6:407245/136‑1 (MQ=11)

                                                                                                                           cAATGTGTTTCGTTGGATCTTCAGGAATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAAAAGCAAGATAGc         <  1:87663/138‑1 (MQ=11)

                                                                                                                           cAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCTGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCATGATAGc         <  6:210361/138‑1 (MQ=11)

                                                                                                                           cAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCa        <  5:167595/139‑1 (MQ=255)

                                                                                                                             aTGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCa                                                             <  2:352399/84‑1 (MQ=18)

                                                                                                                                tgtATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTacaac                    >  7:119703/1‑122 (MQ=9)

                                                                                                                                tgtATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTacaac                    <  8:119703/122‑1 (MQ=9)

                                                                                                                                  tATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTaa  <  6:342132/138‑1 (MQ=255)

                                                                                                                                  tATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTaa  <  1:417590/138‑1 (MQ=255)

                                                                                                                                  tATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTa   >  8:254208/1‑137 (MQ=255)

                                                                                                                                  tATCCTTGGATCTTAAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCAAAAATCTTTTTTAAGTTCTGGAGATGCAGCTAATACTACAATTCCCTGTCACTCCGTATTCCCTACAAAAGCAAGATAGCATGATaa  >  8:177582/1‑138 (MQ=11)

TTCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATTCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAACACTACACTTCCCTGTCTATCCGTATTCCCTACAACAGCAAGATAGCATGTTAA  >  BS168_reference_genome/2271291‑2271558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`