Predicted mutation
evidence seq id position mutation annotation gene description
RA BS168_reference_genome 3,474,002 A→G I211V (ATC→GTC)  yvbI → conserved protein of unknown function

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome3,474,0020AG98.7% 277.6 / ‑4.4 75I211V (ATC→GTC) yvbIconserved protein of unknown function
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (38/36);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

CTTTACCATATTAGCCGTTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCATCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTATTGAATAGACGTTAAGTAAATTCTTCCG  >  BS168_reference_genome/3473872‑3474136
                                                                                                                                  |                                                                                                                                      
cTTTACCATATTAGCCGTTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGt                                                                                                                                 <  5:136393/138‑1 (MQ=255)
   tACCATATTAGCCGTTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACa                                                                                                                             >  5:454647/1‑139 (MQ=255)
   tACCATATTAGCCGTTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACa                                                                                                                             >  3:357335/1‑139 (MQ=255)
   tACCATATTAGCCGTTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACa                                                                                                                             >  6:240884/1‑139 (MQ=255)
     ccATATTAGCCGTTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGATGGTTGATTTCATTTAATCAACGATTGTGATTTATCTGATCGTCTGCGATAAAtt                                                                                                                           >  8:151439/1‑139 (MQ=255)
      cATATTAGCCGGTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGAGCGTCTGCGTTACAttt                                                                                                                          <  8:169956/139‑1 (MQ=255)
           tAGCCGTTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCt                                                                                                                     >  3:419896/1‑139 (MQ=255)
            aGCCGTTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCt                                                                                                                     <  1:92453/138‑1 (MQ=255)
            aGCCGTTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCt                                                                                                                     <  5:240884/138‑1 (MQ=255)
             gCCGTTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCtta                                                                                                                   <  5:239004/139‑1 (MQ=255)
             gCCGTTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCtta                                                                                                                   <  7:197093/139‑1 (MQ=255)
             gCCGTTGGTGTAATCGGTGCATTCATCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCtta                                                                                                                   <  4:274041/139‑1 (MQ=255)
             gCCGGTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCtta                                                                                                                   <  5:285976/139‑1 (MQ=255)
              ccGTTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCt                                                                                                                     <  5:144466/136‑1 (MQ=255)
              ccGTTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCt                                                                                                                     >  6:144466/1‑136 (MQ=255)
                gTTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCttatta                                                                                                                >  5:105222/1‑139 (MQ=255)
                  tGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGTGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCACCTTATTATc                                                                                                              >  8:212977/1‑139 (MQ=255)
                  tGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATc                                                                                                              >  3:91077/1‑139 (MQ=255)
                  tGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCAATCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATATTATCGTCTGCGTTAAATTTCAGCTTATTATc                                                                                                              >  2:216503/1‑139 (MQ=255)
                     tttAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGaa                                                                                                           <  1:128752/139‑1 (MQ=255)
                       tAATCGGTGCATTCTTCGCCATTCCGCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTAATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAac                                                                                                          >  6:405041/1‑138 (MQ=255)
                        aaTCGGTCCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAaca                                                                                                         <  4:130772/138‑1 (MQ=255)
                              tGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCg                                                                                                             <  3:166735/128‑1 (MQ=255)
                              tGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCg                                                                                                             >  4:166735/1‑128 (MQ=255)
                              tGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGt                                                                                                   >  7:394293/1‑138 (MQ=255)
                               gCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCa                                                                                                 <  6:454647/139‑1 (MQ=255)
                                         ccATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAAtttt                                                                                       <  2:100560/139‑1 (MQ=255)
                                         ccATTCCTCCTGCGATGCTGAGCAGTTAACAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAAtttt                                                                                       <  6:250658/139‑1 (MQ=255)
                                          cATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTc                                                                                      >  5:248189/1‑139 (MQ=255)
                                            ttCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCg                                                                                    >  2:164474/1‑139 (MQ=255)
                                                  ctGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTtat                                                                                <  1:164474/137‑1 (MQ=255)
                                                    gCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGatcgtc                                                                                                                                      >  6:364084/1‑81 (MQ=255)
                                                    gCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGatcgtc                                                                                                                                      <  5:364084/81‑1 (MQ=255)
                                                     cGATGCTGAGCAGTTACCATCATTCATATAAAGGGAAGGTTGATTTCATTTACTCTACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATg                                                                             <  7:151439/137‑1 (MQ=255)
                                                     cGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGct                                                                           <  3:422966/139‑1 (MQ=255)
                                                             aGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTaaa                                                                   <  3:132134/139‑1 (MQ=255)
                                                             aGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTaa                                                                    >  5:280435/1‑138 (MQ=255)
                                                                  ttACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAaacaa                                                              >  5:196088/1‑139 (MQ=255)
                                                                    aCCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAAcc                                                            >  7:279781/1‑139 (MQ=255)
                                                                    aCCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAAcc                                                            >  4:58909/1‑139 (MQ=255)
                                                                    aCCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATGATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAAcc                                                            >  7:273024/1‑139 (MQ=255)
                                                                       aGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCa                                                         <  8:394293/139‑1 (MQ=255)
                                                                         cATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCAt                                                        >  4:128430/1‑138 (MQ=255)
                                                                         cATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCAt                                                        >  5:135873/1‑138 (MQ=255)
                                                                           ttCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCAc                                                     <  5:176201/139‑1 (MQ=255)
                                                                            tCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAaacaac                                                             >  4:307682/1‑130 (MQ=255)
                                                                            tCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAaacaac                                                             <  3:307682/130‑1 (MQ=255)
                                                                            tCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCAcc                                                    >  7:165045/1‑139 (MQ=255)
                                                                              atatAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACctg                                                  >  6:13521/1‑139 (MQ=255)
                                                                               tataAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACctgc                                                 <  3:128430/139‑1 (MQ=255)
                                                                                   aaGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCt                                                                                                                     >  7:285084/1‑67 (MQ=255)
                                                                                   aaGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCt                                                                                                                     <  8:285084/67‑1 (MQ=255)
                                                                                   aaGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGt                                             >  2:195667/1‑139 (MQ=255)
                                                                                   aaGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGt                                             >  7:309823/1‑139 (MQ=255)
                                                                                     gggAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGa                                           <  6:135873/139‑1 (MQ=255)
                                                                                        aaGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCATCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATgg                                        >  2:27391/1‑139 (MQ=255)
                                                                                        aaGGTTGATTTCATTTACTCAACGATTGGGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCCTTATATTCTCTTTTAAAAACAAACCCATCAAATGCAGGTGATgg                                        >  5:303262/1‑139 (MQ=255)
                                                                                          ggTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATgggg                                      <  8:135909/139‑1 (MQ=255)
                                                                                          ggTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCAACACCTGCTGGTGATgggg                                      >  4:92062/1‑139 (MQ=255)
                                                                                           gTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAACAAATTATCCGATAAATGCTCTTTTAAAAACAACCCCAACACCTGCTGGAGATGGGgt                                     <  3:464490/139‑1 (MQ=255)
                                                                                                tttCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCtttt                                                                      <  4:88409/101‑1 (MQ=255)
                                                                                                tttCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCtttt                                                                      >  3:88409/1‑101 (MQ=255)
                                                                                                tttCATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGgttgtt                                 <  6:55088/138‑1 (MQ=255)
                                                                                                   cATTTACTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTa                              <  4:357335/138‑1 (MQ=255)
                                                                                                        aCTCAACGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTATTGaa                         <  5:13521/138‑1 (MQ=255)
                                                                                                             aCGATTGTGATTTATCTGATCGTCTTCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTATTGAATAGACg                   <  5:38623/139‑1 (MQ=255)
                                                                                                              cGATTGTGATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTATTGAATAGACGt                  >  1:198731/1‑139 (MQ=255)
                                                                                                                  tgtgATTTATCTGATCGTCTGAGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTATTGAATAGACGTTaa               <  8:299584/138‑1 (MQ=255)
                                                                                                                    tgATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTATTGAATAGACGTTAAGt             >  3:114053/1‑138 (MQ=255)
                                                                                                                    tgATTTATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCACATCACCTGCTGGTGATGGGGTTGTTTTATTGAATATAGGTTAAGt             >  6:45810/1‑138 (MQ=255)
                                                                                                                        ttATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTC‑AAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTa                              >  6:379823/1‑116 (MQ=255)
                                                                                                                        ttATCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTC‑AAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTa                              <  5:379823/116‑1 (MQ=255)
                                                                                                                          aTCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTATTGAATAGACGTTAAGTAAAttct      >  7:444441/1‑139 (MQ=255)
                                                                                                                           tCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTTTTGAATAGACGTTAAGTAAAttctt     >  4:164821/1‑139 (MQ=255)
                                                                                                                           tCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTATTGAATa                       >  6:248066/1‑121 (MQ=255)
                                                                                                                           tCTGATCGTCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTATTGAATa                       <  5:248066/121‑1 (MQ=255)
                                                                                                                            cTGATCGTCTGCGTTACATTGCAGCTTATTATCGAACACTATGTCAACGAAATTTTCCGTTATCTGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTATTGAATAGACGTTAAGTAAAttctt     <  8:273024/138‑1 (MQ=255)
                                                                                                                               atcgtcTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCa                                                         >  1:46055/1‑83 (MQ=255)
                                                                                                                               atcgtcTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCa                                                         <  2:46055/83‑1 (MQ=255)
                                                                                                                               atcgtcTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTATTGAATAGACGTTAAGTAAAttctt     <  8:279781/135‑1 (MQ=255)
                                                                                                                               atcgtcTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTATTGAATAGACGTTAAGTAAATTCTTCCg  <  4:419896/138‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                      
CTTTACCATATTAGCCGTTGGTTTAATCGGTGCATTCTTCGCCATTCCTCCTGCGATGCTGAGCAGTTACCAGCATTCATATAAAGGGAAGGTTGATTTCATTTACTCAACGATTGTGATTTATCTGATCATCTGCGTTACATTTCAGCTTATTATCGAACACTATGTCAAAGAAATTTTCCGTTATATGCTCTTTTAAAAACAACCCCATCACCTGCTGGTGATGGGGTTGTTTTATTGAATAGACGTTAAGTAAATTCTTCCG  >  BS168_reference_genome/3473872‑3474136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑