Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	718,125	T→C	Y78C (TAT→TGT)	yerD ←	putative osmotic shock glutamate synthase subunit (flavoprotein subunit, ferredoxin‑dependent)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	718,125	0	T	C	100.0%	321.6 / NA	84	Y78C (TAT→TGT)	yerD	putative osmotic shock glutamate synthase subunit (flavoprotein subunit, ferredoxin‑dependent)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/48);  total (36/48)

CATTTCTTCCCTTTGTTTTGGAAACATAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCATATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCTTCCGATGACGGGATAGTTGCGAAGAATTGAGTGCTCCTG  >  BS168_reference_genome/718001‑718258                                                                                                                             |                                                                                                                                      cATTTCTTCCCTTTGTTTTGGAAACATAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGaa                                                                                                                         <  6:272548/139‑1 (MQ=255)         cccTTTGTTTTGGAAACATAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATgg                                                                                                                    <  2:364653/136‑1 (MQ=255)         cccTTTGTTTTGGAAACATAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGgct                                                                                                                  <  2:54876/138‑1 (MQ=255)          ccTTTGTTTTGGAAACATAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGgctg                                                                                                                 <  6:293564/138‑1 (MQ=255)          ccTTTGTTTTGGAAACATAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGgctg                                                                                                                 <  3:299771/138‑1 (MQ=255)          ccTTTGTTTTGGAAACATAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGgctg                                                                                                                 <  2:294836/138‑1 (MQ=255)           cTTTGTTTTGGAAACATAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGgctgct                                                                                                               >  6:457395/1‑139 (MQ=255)           cTTTGTTTTGGAAACATAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGgctgct                                                                                                               >  6:450783/1‑139 (MQ=255)            tttGTTTTGGAAACATAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGctc                                                                                                              >  6:294204/1‑139 (MQ=255)              tGTTTTGGAAACATAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCtt                                                                                                            >  7:112822/1‑139 (MQ=255)                         cATAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGCGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTtcattgtcattg                                                                                                 <  8:441497/139‑1 (MQ=255)                          aTAGCTTTACGGATATTATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATTACGGATTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  7:43927/139‑1 (MQ=255)                          aTAGCGTTACGGATATAATATCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  7:359257/139‑1 (MQ=255)                          aTAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACGGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  6:362403/139‑1 (MQ=255)                          aTAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  5:393832/139‑1 (MQ=255)                          aTAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  6:232143/139‑1 (MQ=255)                          aTAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  7:11506/139‑1 (MQ=255)                          aTAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  7:236098/139‑1 (MQ=255)                          aTAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  1:206974/139‑1 (MQ=255)                          aTAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  7:87750/139‑1 (MQ=255)                          aTAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  1:100711/139‑1 (MQ=255)                          aTAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGCTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  6:374741/139‑1 (MQ=255)                          aTAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGATTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  5:427398/139‑1 (MQ=255)                          aTAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTAATTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  1:379294/139‑1 (MQ=255)                          aTAGAGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCAGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  6:419506/139‑1 (MQ=255)                                   cGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTAGaaa                                                                                        >  4:37331/1‑138 (MQ=255)                                    ggATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATa                                                                                      <  2:76483/139‑1 (MQ=255)                                     gATATCATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATAc                                                                                     <  5:450783/139‑1 (MQ=255)                                     gATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATa                                                                                      >  7:195701/1‑138 (MQ=255)                                     gATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATa                                                                                      <  8:195701/138‑1 (MQ=255)                                     gATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATAc                                                                                     <  5:457395/139‑1 (MQ=255)                                     gATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATAc                                                                                     <  4:197841/139‑1 (MQ=255)                                     gATATAATAGCCAGGTTTATCAAAATATCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGTTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATAc                                                                                     <  4:410734/139‑1 (MQ=255)                                        ataataGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAAt                                                                                       <  4:238078/134‑1 (MQ=255)                                        ataataGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAAt                                                                                       >  3:238078/1‑134 (MQ=255)                                        ataataGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTg                                                                                   >  3:105411/1‑138 (MQ=255)                                               ccAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGgctgc                                                                                                                <  7:66355/102‑1 (MQ=255)                                               ccAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGgctgc                                                                                                                >  8:66355/1‑102 (MQ=255)                                               ccAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                >  4:6616/1‑118 (MQ=255)                                               ccAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                >  4:428905/1‑118 (MQ=255)                                               ccAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  3:428905/118‑1 (MQ=255)                                               ccAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  3:6616/118‑1 (MQ=255)                                               ccAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCt                                                                           >  1:112377/1‑139 (MQ=255)                                               ccAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCt                                                                           >  7:44538/1‑139 (MQ=255)                                               ccAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCt                                                                           >  7:111535/1‑139 (MQ=255)                                               ccAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCATGAGATGAAGGTTTGCTCACATTCCTTTCTTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTGTGAGCt                                                                           >  3:236489/1‑139 (MQ=255)                                               ccAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGATTTTGAATTTGCCGGAGATGACGGTTAGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTTCTGTATAAATAATGACTGAGCt                                                                           >  8:181916/1‑139 (MQ=255)                                                 aGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTcc                                                                         >  6:159038/1‑139 (MQ=255)                                                                  ttACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTc                                                           <  1:417529/136‑1 (MQ=255)                                                                  ttACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCa                                                          <  5:294204/137‑1 (MQ=255)                                                                  ttACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGACGGAGATGACGGTGTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGAtttttt                                                            <  8:246692/135‑1 (MQ=255)                                                                     cAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGa                                                      <  6:351995/138‑1 (MQ=255)                                                                     cAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGa                                                      <  2:282278/138‑1 (MQ=255)                                                                        aTCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGta                                                                                            >  1:151753/1‑97 (MQ=255)                                                                        aTCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGta                                                                                            <  2:151753/97‑1 (MQ=255)                                                                        aTCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGAtata                                                  >  6:374494/1‑139 (MQ=255)                                                                        aTCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGAtata                                                  >  4:358931/1‑139 (MQ=255)                                                                         tCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAATTACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATAc                                                 >  3:271117/1‑139 (MQ=255)                                                                          ccAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCa                                                          <  1:280162/129‑1 (MQ=255)                                                                          ccAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCa                                                          >  2:280162/1‑129 (MQ=255)                                                                          ccAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATAcc                                                >  5:53655/1‑139 (MQ=255)                                                                            aaaGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGa                                              <  2:112377/139‑1 (MQ=255)                                                                                  catcatTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                >  6:386840/1‑83 (MQ=255)                                                                                  catcatTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGc                                                                                                <  5:386840/83‑1 (MQ=255)                                                                                  catcatTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCtt                                        >  3:324188/1‑139 (MQ=255)                                                                                  catcatTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCtt                                        >  8:345311/1‑139 (MQ=255)                                                                                    tcatTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCATGGATTTACCGAAACCTTcc                                      >  8:188926/1‑139 (MQ=255)                                                                                    tcatTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCTTcc                                      >  2:244877/1‑139 (MQ=255)                                                                                        tCTGCTTTTGAATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAATATGGCTGCTCTTCATTGTCATTGCTTTATAAATACTGTCTGTGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCTTCCGAt                                   <  7:181916/138‑1 (MQ=255)                                                                                          tGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCTTACGATGAc                                >  1:174386/1‑139 (MQ=255)                                                                                            cTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCt                                                                           <  2:18045/94‑1 (MQ=255)                                                                                            cTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCt                                                                           >  1:18045/1‑94 (MQ=255)                                                                                            cTTTTGTATTTGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCTTCCGATGa                                 <  4:105411/136‑1 (MQ=255)                                                                                                    tttGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCTTCCGATGACGGGATAGtt                       <  8:112822/138‑1 (MQ=255)                                                                                                    tttGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCTTCCGATGACGGGATAGTTg                      >  2:382316/1‑139 (MQ=255)                                                                                                      tGCCGGAGATGACGGTTTGCTCCCATTCCTTTCGGGAAAATGGCTGCTCGTCAGTGGCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAACGATATACCGCAACCTTCCGATGCCGGGATAGTTggt                    >  7:433279/1‑137 (MQ=255)                                                                                                      tGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCTTCCGATGACGGGATAGTTGc                     >  1:435194/1‑138 (MQ=255)                                                                                                      tGCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCTTCCGATGACGGGATAGTTGc                     >  3:454119/1‑138 (MQ=255)                                                                                                       gCCGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCTTCCGATGACGGGATAGTTGCg                    <  5:374494/138‑1 (MQ=255)                                                                                                        ccGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCTTCCGATGACGGGATAGTTGCgaa                  <  5:159038/139‑1 (MQ=255)                                                                                                        ccGGAGATGACGGTTTGCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCTTCCGATGACGGGATAGTTGCgaa                  <  6:53655/139‑1 (MQ=255)                                                                                                                        gCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCTTCCGATGACGGGATAGTTGCga                   >  5:397381/1‑122 (MQ=255)                                                                                                                        gCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCTTCCGATGACGGGATAGTTGCga                   <  6:397381/122‑1 (MQ=255)                                                                                                                        gCTCACATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCTTCCGATGACGGGATAGTTGCGAAGAATTGAGTGCTCctg  >  8:396125/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                      CATTTCTTCCCTTTGTTTTGGAAACATAGCGTTACGGATATAATAGCCAGGTTTATCAAAATCTCTTACAGATCCAAAGCCCATCATTCTGCTTTTGTATTTGCCGGAGATGACGGTTTGCTCATATTCCTTTCGTGAAAATGGCTGCTCTTCATTGTCATTGCTGTATAAATACTGTCTGAGCTCCGGCCCGATTTTTTCAAGGATATACCGAAACCTTCCGATGACGGGATAGTTGCGAAGAATTGAGTGCTCCTG  >  BS168_reference_genome/718001‑718258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑