mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Marginal read alignment evidence (highest frequency 20 of 77 shown, sorted by frequency from high to low) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 1,820,728 | 0 | G | A | 52.6% | ‑0.8 / 115.8 | 78 | D4270N (GAT→AAT) | pksL | polyketide synthase of type I (bacillaene synthesis) |
| * | BS168_reference_genome | 2,003,218 | 0 | G | T | 51.1% | 16.7 / 54.5 | 45 | intergenic (+36/+58) | iseA/trnSL‑Arg1 | inhibitor of DL‑endopeptidases involved in cell‑separation/tRNA‑Arg |
| * | BS168_reference_genome | 608,214 | 1 | . | A | 50.0% | 47.2 / 84.0 | 46 | intergenic (‑139/+32) | ydgF/dinB | putative amino acid permease/nuclease inhibitor |
| * | Exported | 4,137 | 0 | C | T | 49.2% | 1330.6 / inf | 8122 | L89L (CTC→CTT) | ampR | /vntifkey=4 |
| * | BS168_reference_genome | 2,003,209 | 0 | T | G | 48.9% | 8.3 / 36.0 | 47 | intergenic (+27/+67) | iseA/trnSL‑Arg1 | inhibitor of DL‑endopeptidases involved in cell‑separation/tRNA‑Arg |
| * | BS168_reference_genome | 911,959 | 0 | T | G | 47.8% | ‑2.8 / 13.9 | 23 | intergenic (+31/+5) | icaC/yfiR | acetyl‑glucosamine metabolite exporter component involved in biofilm formation/transcriptional regulator (TetR/AcrR family) |
| * | Exported | 4,114 | 0 | A | G | 40.5% | 4115.8 / inf | 6732 | I82V (ATT→GTT) | ampR | /vntifkey=4 |
| * | BS168_reference_genome | 515,039 | 0 | G | T | 37.0% | 20.5 / 10.4 | 30 | Q193K (CAA→AAA) | ydcA | putative rhomboid protease |
| * | BS168_reference_genome | 223,363 | 0 | C | G | 36.6% | 71.0 / 13.3 | 44 | K209N (AAG→AAC) | ybdM | putative protein kinase |
| * | BS168_reference_genome | 2,748,690 | 0 | T | G | 35.8% | 64.2 / 16.3 | 67 | K43T (AAG→ACG) | csn | chitosanase |
| * | BS168_reference_genome | 3,335,756 | 0 | A | C | 35.5% | 32.2 / 11.2 | 31 | F173V (TTT→GTT) | pucE | xanthine dehydrogenase, iron‑sulfur subunit |
| * | BS168_reference_genome | 1,023,102 | 0 | C | A | 35.1% | 81.8 / 13.6 | 57 | intergenic (+2/‑248) | yhdF/bcaP | putative NAD(P)‑dependent dehydrogenase/branched‑chain amino acid transporter or methylthioribose‑degradation metabolite exporter |
| * | BS168_reference_genome | 3,333,277 | 0 | T | G | 34.9% | 50.0 / 20.9 | 43 | F39C (TTT→TGT) | pucL | urate oxidase with peroxide reductase N‑terminal domain |
| * | BS168_reference_genome | 2,003,214 | 0 | A | T | 34.8% | 12.9 / 24.9 | 46 | intergenic (+32/+62) | iseA/trnSL‑Arg1 | inhibitor of DL‑endopeptidases involved in cell‑separation/tRNA‑Arg |
| * | BS168_reference_genome | 463,394 | 0 | C | A | 34.4% | 79.7 / 23.1 | 64 | R50R (CGC→CGA) | yczI | conserved protein of unknown function |
| * | BS168_reference_genome | 1,511,930 | 0 | G | T | 34.3% | 25.9 / 10.1 | 35 | S88* (TCA→TAA) | ykoA | putative membrane protein of unknown function |
| * | BS168_reference_genome | 3,918,051 | 0 | A | T | 34.1% | 55.1 / 12.5 | 44 | Y71N (TAC→AAC) | qoxA | cytochrome aa3‑600 quinol oxidase (subunit II) |
| * | BS168_reference_genome | 608,185 | 0 | G | T | 33.9% | 106.4 / 10.4 | 61 | intergenic (‑110/+61) | ydgF/dinB | putative amino acid permease/nuclease inhibitor |
| * | BS168_reference_genome | 238,232 | 0 | T | G | 33.3% | 46.8 / 23.1 | 54 | pseudogene (217/285 nt) | ybfEc | conserved protein of unknown function (C‑terminal end) |
| * | BS168_reference_genome | 824,692 | 0 | T | G | 32.6% | 61.3 / 17.5 | 46 | K9T (AAA→ACA) | fecE | iron‑dicitrate ABC transporter (permease) |
| Marginal new junction evidence (sorted from low to high skew) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | BS168_reference_genome | = 3485387 | 106 (1.140) | 39 (0.020) | 28/242 | 1.2 | 2.4% | coding (80/1395 nt) | araE | arabinose‑related compounds permease |
| ? | Exported | = 5546 | 3032 (0.700) | intergenic (+815/‑342) | ampR/lacZalpha | /vntifkey=4//vntifkey=4 | |||||
| * | ? | BS168_reference_genome | 3484069 = | 92 (0.990) | 25 (0.010) +34 bp |
13/184 | 2.3 | 2.2% | intergenic (‑295/+3) | cggR/araE | transcriptional regulator of gapA/arabinose‑related compounds permease |
| ? | Exported | 6207 = | 2936 (0.650) | coding (320/324 nt) | lacZalpha | /vntifkey=4 | |||||
| * | ? | BS168_reference_genome | = 2611434 | 91 (0.980) | 7 (0.000) | 7/252 | 4.9 | 0.53% | intergenic (‑97/+22) | cdd/unpK | cytidine/deoxycytidine deaminase/undecaprenol kinase |
| ? | Exported | 5613 = | 2524 (0.560) | intergenic (+882/‑275) | ampR/lacZalpha | /vntifkey=4//vntifkey=4 | |||||
| * | ? | BS168_reference_genome | = 9501 | 103 (1.110) | 6 (0.000) | 5/252 | 5.6 | 0.42% | intergenic (+42/‑309) | gyrA/rrnO‑16S | DNA gyrase (subunit A)/ribosomal RNA‑16S |
| ? | Exported | 6212 = | 2766 (0.620) | intergenic (+1/–) | lacZalpha/– | /vntifkey=4/– | |||||
| * | ? | BS168_reference_genome | 2653329 = | 85 (0.910) | 5 (0.060) | 3/242 | 6.4 | 8.2% | pseudogene (337/471 nt) | sigKn | RNA polymerase sporulation‑specific sigma‑K factor precursor (Sigma‑27) (N‑terminal half) |
| ? | BS168_reference_genome | = 2701361 | 31 (0.350) | pseudogene (24/417 nt) | sigKc | RNA polymerase sporulation‑specific sigma‑K factor precursor (Sigma‑27) (C‑terminal fragment) | |||||
| * | ? | BS168_reference_genome | 4171249 = | 105 (1.130) | 3 (0.000) | 3/250 | 6.5 | 0.11% | coding (1205/1308 nt) | yybO | putative permease |
| ? | Exported | 1541 = | 5293 (1.190) | intergenic (–/‑1437) | –/eryR | –//vntifkey=4 | |||||
| * | ? | BS168_reference_genome | 1829200 = | 122 (1.310) | 4 (0.000) | 3/250 | 6.5 | 0.21% | coding (7648/12789 nt) | pksM | trans AT polyketide synthase of type I involved in bacillaene synthesis |
| ? | Exported | 5197 = | 3639 (0.820) | intergenic (+466/‑691) | ampR/lacZalpha | /vntifkey=4//vntifkey=4 | |||||