| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,578,466 | Δ1 bp | 100% | intergenic (‑59/‑76) | U712_18365 ← / → U712_18370 | Stage II sporulation protein Q/Putative phosphinothricin acetyltransferase YwnH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,578,466 | 0 | A | . | 100.0% | 166.8 / NA | 37 | intergenic (‑59/‑76) | U712_18365/U712_18370 | Stage II sporulation protein Q/Putative phosphinothricin acetyltransferase YwnH |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (17/20); total (17/20) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCAAAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGT > CP006881/3578329‑3578588 | ttGCAGGGAACACACAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGAATTGAGAAGTTTTCTTTTCTTCCTCTCTCAGTGTTTCATAAACTCATCAACATTCTGACCACTTTTATGAAAATATATACAACATATACa > 5:118470/1‑138 (MQ=255) ggAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCaaaaaact > 7:264335/1‑137 (MQ=255) acCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTACTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTATGAAAATATATACAACCTATCC‑AAAAAACtttttt > 6:330540/1‑139 (MQ=255) aGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGAt < 1:239178/139‑1 (MQ=255) aGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGAt < 5:18429/139‑1 (MQ=255) gTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTa > 1:79375/1‑139 (MQ=255) tACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTGTTGCTCTCTCATTGTTTGATCACCTCAGCAACATTCAGTACACTTTTCTGTAAATATATACAACCTATAC‑AAAAAACTTTTTTAGATGTAGtt > 5:54707/1‑139 (MQ=255) gAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACAATATGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTATTTTAGATGTAGtt > 3:258524/1‑136 (MQ=255) aaaaaaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGca < 8:103369/139‑1 (MQ=255) ctgAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGaa > 5:353214/1‑139 (MQ=255) aCTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTa < 4:365436/104‑1 (MQ=255) aCTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTa > 3:365436/1‑104 (MQ=255) cTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTAtt < 8:386873/139‑1 (MQ=255) ccTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCaaa < 1:388192/138‑1 (MQ=255) tcATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCaaaaa < 3:38423/134‑1 (MQ=255) tcATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGt < 5:330540/137‑1 (MQ=255) aTTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATg < 7:338543/138‑1 (MQ=255) tttCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACa < 1:142659/138‑1 (MQ=255) tttCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACa < 1:136692/138‑1 (MQ=255) tttCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACa < 2:79375/138‑1 (MQ=255) tcatcaCCTCCGCAACATTCTGAACACTTTTCTGAAATTAGATTCACCCTATCC‑AAACAACGTTTTTATATGTAGTTTGCACAGACTGAACGAAAATATGTAGTTTTTTGAGAGGAGTGGCAAAAACGTAGGATCATGa > 2:20667/1‑139 (MQ=255) atcaCCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACa < 5:140419/139‑1 (MQ=255) aCCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGAATGAACATGACa > 4:227174/1‑136 (MQ=255) ccTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGc > 6:242526/1‑139 (MQ=255) cTCAGCAACATTCTGAACACTATTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACAtt < 4:332380/136‑1 (MQ=255) aCATTCTTAACACTTTTCTGAAAATATATACAAACTATCC‑AAAAAACTTTTTTATCTGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAAAGTATGAACATGACATTGCGTCTTGCt < 6:118470/139‑1 (MQ=255) aCATTCTGAACACTTTTCTGAAAATATATACCACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCt < 6:24245/139‑1 (MQ=255) cATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTg < 8:264335/139‑1 (MQ=255) ttCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGaa > 5:199267/1‑139 (MQ=255) ttCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGa > 2:141573/1‑138 (MQ=255) tGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGaa > 3:399710/1‑136 (MQ=255) tGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGaa > 4:325587/1‑136 (MQ=255) aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAAc < 3:325587/135‑1 (MQ=255) ttttCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCaaa < 7:387598/97‑1 (MQ=255) ttttCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCaaa > 8:387598/1‑97 (MQ=255) ttttCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACt > 5:114890/1‑139 (MQ=255) aaaaTATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa < 7:259470/126‑1 (MQ=255) aaaaTATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa > 8:259470/1‑126 (MQ=255) atatatACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCtgt > 2:8136/1‑139 (MQ=255) | TTGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCAAAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGT > CP006881/3578329‑3578588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |