Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	732,264	A→C	13.0%	S135R (AGC→CGC)	U712_03550 →	Uncharacterized protein yesU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	732,264	0	A	C	13.0%	175.1 / 5.1	77	S135R (AGC→CGC)	U712_03550	Uncharacterized protein yesU
Reads supporting (aligned to +/- strand):  ref base A (37/29);  new base C (2/9);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 2.49e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTGACCCAAGTCTCAGGAAAAGAACAGGAACATACCCTGAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTATGTGCACTTTTCAATCAATGGTCTGCCCATTCTTGAG  >  CP006881/732128‑732401                                                                                                                                          |                                                                                                                                          ttttgACCCAAGTATCAGTAAAAGAACAGGAACATACCCTGAGTATCATTCAGGAGATATAAATGCCCT‑CCACCTGTCGTATTTCCGCATAAAATACGCGGAGTAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAg                                                                                                                                          <  8:172490/137‑1 (MQ=255)    tgACCCAAGTCTCAGGAAAAGAACAGGAACATACCCTGAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCcg                                                                                                                                       <  5:46937/138‑1 (MQ=255)      aCCCAAGTCTCAGGAAAAGAACAGGAACATACCCTGAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCcgcg                                                                                                                                     <  7:315603/138‑1 (MQ=255)       cccAAGTCTCAGGAAAAGAACAGGAACATACCCTGAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGt                                                                                                                                   >  8:354424/1‑139 (MQ=255)            gTCTCAGGAAAAGAACAGGAACATACCCTGAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCa                                                                                                                              >  7:234044/1‑139 (MQ=255)             tctcAGGAAAAGAACAGGAACATACCCTGAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCAt                                                                                                                             <  7:341745/139‑1 (MQ=255)                 agcaaaaGACCAGGAAAATACCCTGATTATCATTCAGGAGATATCAATGCCCT‑CAACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAATAGCGTACCGCACCTGCATTTTAAGAAAAAGCCGCGGTTTCCATCTcg                                                                                                                         <  6:277840/136‑1 (MQ=255)                 aGGTAAAGAACAGGAACATACCCTGATTATCATTCAGTTCATATCAATGCCCT‑CCACCTGTCGTATTTCCCCACAAAATACGCGTAGGAAAGAGCTTTCCGCACCTGAAATTTAAGAAAAAGCCGCGGTTTCCAtct                                                                                                                           <  2:158008/137‑1 (MQ=255)                 aGGAAAAGAACAGGAACATACCCTGAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTcg                                                                                                                         >  6:422130/1‑139 (MQ=255)                         aaCAGGAACATACCCTGAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTcgccgc                                                                                                                     >  3:241046/1‑135 (MQ=255)                              gAACATACCCTGAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGc                                                                                                            >  2:379988/1‑139 (MQ=255)                                   tACCCTGAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATc                                                                                                       >  7:367430/1‑139 (MQ=255)                                        tGAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTg                                                                                                  >  1:150904/1‑139 (MQ=255)                                        tGAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTg                                                                                                  >  7:271117/1‑139 (MQ=255)                                        tGAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTg                                                                                                  >  5:366151/1‑139 (MQ=255)                                         gAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGCTCCGCTGc                                                                                                 >  8:202093/1‑139 (MQ=255)                                         gAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCg                                                                                                     <  6:307393/135‑1 (MQ=255)                                         gAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCg                                                                                                     >  5:307393/1‑135 (MQ=255)                                         gAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGc                                                                                                 >  8:384872/1‑139 (MQ=255)                                          aGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGCGCGTTCCGCACCTGCAATTTAAGAAACCGCCGCGGTTTCCAGCTCGCCGCAATTGGGGCCGAGCCGCTGcc                                                                                                >  6:415165/1‑139 (MQ=255)                                          aGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGcc                                                                                                <  8:271117/139‑1 (MQ=255)                                          aGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAAT‑GGGGCCGATCCGCTGCCt                                                                                               >  1:263438/1‑139 (MQ=255)                                            tATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTTTTTCCGCAGAAAATACGCGGAGGAACGAGCGTTCCGCCCCTGCAAGTTAAGAAAACGCCGCGGTTTCCAGCTCGCCGCAATGGGGGCCGCTCCGCGGc                                                                                                 >  4:103694/1‑136 (MQ=255)                                             aTCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGcc                                                                                                >  3:76763/1‑136 (MQ=255)                                                aTTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTtctc                                                                                           >  1:18852/1‑138 (MQ=255)                                                aTTCAGGAGATATCAATGCACT‑CCACCTGTCGTATTTACGCAGAAAATACGCGGAGGAAAGATCGTTCCGAACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTtctc                                                                                           >  8:395148/1‑138 (MQ=255)                                                    aGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAgac                                                                                      >  5:215868/1‑139 (MQ=255)                                                       agaTATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAgac                                                                                      >  3:246225/1‑136 (MQ=255)                                                        gaTATCCATGCCCT‑CCCCCTGGCGTATTTCCGCAGAAACTACGGGGAGGAAAGAGCGTGCCGCACCTGAAATTTAAGAAAACGCCGCGGTTGCCATCTCGCCGCAAGGGGGGCCGATCCGCTGCCTTCTCCCGacgacg                                                                                  <  8:291866/139‑1 (MQ=255)                                                        gaTATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAgacg                                                                                     <  3:204113/136‑1 (MQ=255)                                                         atatCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGcc                                                                                                <  6:185164/124‑1 (MQ=255)                                                         atatCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGcc                                                                                                >  5:185164/1‑124 (MQ=255)                                                         atatCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGc                                                                                 >  6:391383/1‑139 (MQ=255)                                                         atatCAATGCCCT‑CAACCTGTCGTATTTCCGCAGAATATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGTGTTCCCTCTCGCCGCAATGTGGGCCGCTCCGACCCCTTCTCCAgacgc                                                                                    >  3:70818/1‑135 (MQ=255)                                                           atCAAAGCCCT‑CCACCTGTCGTATTTACGCAGAAAATACGCGGACGAAAGAGCGTTCCGCACCTGCAAATTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATACGCTGCCTACTCCAGACGACGCt                                                                                >  7:14873/1‑138 (MQ=255)                                                                tGCCCT‑CCACCTGTCTTATTTCCGCATAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATtct                                                                          <  6:215868/139‑1 (MQ=255)                                                                tGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGCCGCTGATtct                                                                          >  7:309665/1‑139 (MQ=255)                                                                tGCCCT‑CCACATGTCGTAATTCCGCAGAAAATAAGCGGAGGAAAGAGCGTTCCGCTCCTGCAATTTAAGAAAAAGCCGAGGTTACCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTAGATACGACGCTGAtta                                                                           >  5:133284/1‑137 (MQ=255)                                                                tGCCCT‑CAAACTGTCTTATTTCCGCATAAAATACGGGTAGTAAAGAGCGTTCCGCACCTGAAATTTAATAAAACGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATtct                                                                          <  2:150904/139‑1 (MQ=255)                                                                 gCCCTCCCACCTGTAGATTTCCCGCATAAAAAACGCGCTGTAAACTCTGTGCCGCACCTCAAATAAAATAAAACGCCGCGGTTTCAATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATtct                                                                          <  6:250523/139‑1 (MQ=255)                                                                    cT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCcgcg                                                                                                                                     <  5:409829/76‑1 (MQ=255)                                                                    cT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCcgcg                                                                                                                                     >  6:409829/1‑76 (MQ=255)                                                                    cT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATAATCcg                                                                       >  8:333502/1‑138 (MQ=255)                                                                        cACCTGTGGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTGCCGCACGTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTcc                                                                        <  3:341550/134‑1 (MQ=255)                                                                        cACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCcgta                                                                     <  5:391383/137‑1 (MQ=255)                                                                        cACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCcgta                                                                     <  7:384872/137‑1 (MQ=255)                                                                        cACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCcgta                                                                     <  1:249550/137‑1 (MQ=255)                                                                        cACCTGTCGTATTTCCGCACAAAATACGCGGAGTAAAGCGCGTCCCGCACCTGAAATTAAAGAAAACGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCcgta                                                                     <  8:367430/137‑1 (MQ=255)                                                                        acccTGTGGTATTTCCGCATAAAATACGCGGAGTAAAGAGCGTTCCGCACCTGAAATTTATGAAAACCCCGCGGTTTCCATCTCGCGGCAATGGGGGGCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCcgta                                                                     <  7:311600/135‑1 (MQ=255)                                                                         aCCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGCGCGTTCCGCACCTGCAAGTTTAGCAAAAGCCGCGGATTCGATCTCGCCTCAATGGGGGCCGCTCCGCTGCCTGCTACAGACGACGCGTATTCGCggta                                                                     >  4:157467/1‑136 (MQ=255)                                                                           cTGTCGTATTTCCGCAGAAAATACGCGTTGGAAAGCGCGTGCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCcgtatcgta                                                                <  6:183739/139‑1 (MQ=255)                                                                           cTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCTAGACGACGCTGATTCTCcgtatcgta                                                                <  5:422130/139‑1 (MQ=255)                                                                              tCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATg                                                              >  6:204438/1‑138 (MQ=255)                                                                               cGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCAGCCCCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCGTCTCCAGACGACGCTGATTCTCCGTATCGTATg                                                              >  8:299466/1‑137 (MQ=255)                                                                                gTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTACAGACGACGCGGATTCTCcgtatcgtat                                                               >  4:13534/1‑135 (MQ=255)                                                                                 tATTTCCGCAGAAAATAGGCGGAGGAAAGCGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGc                                                          <  7:230088/139‑1 (MQ=255)                                                                                     tCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGCTCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGACGCTGAt                                                      >  8:50104/1‑139 (MQ=255)                                                                                      ccGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCt                                                         >  3:127675/1‑135 (MQ=255)                                                                                       cGCAGAAACTACGCGGAGCAAAGAGCTGTCCGCACCTGCATTTAAAGAAAACGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCa                                                    <  6:250488/139‑1 (MQ=255)                                                                                       cGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCGGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATc                                                     <  8:234044/138‑1 (MQ=255)                                                                                         cAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGGTCa                                                    >  2:38685/1‑137 (MQ=255)                                                                                         cAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGAGTCTCCGTATCGTATGCTGCTGATCa                                                    >  7:25396/1‑137 (MQ=255)                                                                                             aaaTACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCAGATCAAAGACa                                              <  1:379988/139‑1 (MQ=255)                                                                                              aaTACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAAGGGGGGCCGATCCGCTGCCTTCACCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACaa                                             <  5:137774/139‑1 (MQ=255)                                                                                                        ggaAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTAt                                      <  4:76763/136‑1 (MQ=255)                                                                                                              agCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTATGTGCACttt                             <  2:18852/139‑1 (MQ=255)                                                                                                              agCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTATGTGCACttt                             <  6:112583/139‑1 (MQ=255)                                                                                                                 gTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTATGTGCACTTTtc                           >  1:154645/1‑138 (MQ=255)                                                                                                                   tCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTATGTGCACTTTtcaa                         >  8:329089/1‑138 (MQ=255)                                                                                                                    ccGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTATGTGCACTTTtc                           >  3:359862/1‑135 (MQ=255)                                                                                                                          ctacAATTAAATAAAACGCCGCGGTTTCAATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTATGTGCACTTTTCAATCAATGGt                 <  6:61305/136‑1 (MQ=255)                                                                                                                          cTGCATTTTATCAAAACGCCGCGGTTTCCATCTCGCCGCAAGGGGGGCCGATCGGCGGCCTTATCCCGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTATGTGCACTTTTCAATCAATGGt                 <  5:124045/139‑1 (MQ=255)                                                                                                                          cTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTATGTGCACTTTTCAATCAATGGt                 <  1:36938/139‑1 (MQ=255)                                                                                                                             cAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTATGTGCACTTTTCAATCAATGGTCt               <  7:329089/138‑1 (MQ=255)                                                                                                                              aaTTTAAGAAAAAGCCGCTGTTTCCATCTCGCCGCAATGGGGGCCGAGCTGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTATGTGCACTTTTCAATCAATGGTc                <  4:241046/136‑1 (MQ=255)                                                                                                                              aaTTAAAGAAAAAGCCGCGGTTTCCCTCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTATGTGCACTTTTCAATCAATGGTc                <  4:204067/136‑1 (MQ=255)                                                                                                                               ctttAACAAAACGCCGCGGTTTCCCTCTCGCCGCAAGGGGGGCCGAGCCGCTGCCTTCGCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTATGTGCACTTTTCAATCAATGGTCt               <  3:7610/135‑1 (MQ=255)                                                                                                                                       aaacGCCGCGGTTTCCATCTCGCCGCAAGGGGGGCCGAGCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTATGTGCACTTTTCAATCAATGGTCTGCCCATTCtt     <  7:202093/138‑1 (MQ=255)                                                                                                                                        aaaGCCGCTGGTTCCGGCTCGCCGCAAGGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGAATCTCCGTATCGGATGAAGCTGATCAACGACAAACGCTATGTGCACTTTTCAATCAATGGTCTGCCCATTCt      <  4:95626/136‑1 (MQ=255)                                                                                                                                         aaGCCGCGGGTACCATCACGCCGCAAGGGGGCCCGATCCGCGGCCTGCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTATGTGCACTTTTCAATCAATGGTCTGCCCATTCTTGAg  <  6:46262/139‑1 (MQ=255)                                                                                                                                          |                                                                                                                                          GTTTGACCCAAGTCTCAGGAAAAGAACAGGAACATACCCTGAGTATCATTCAGGAGATATCAATGCCCT‑CCACCTGTCGTATTTCCGCAGAAAATACGCGGAGGAAAGAGCGTTCCGCACCTGCAATTTAAGAAAAAGCCGCGGTTTCCATCTCGCCGCAATGGGGGCCGATCCGCTGCCTTCTCCAGACGACGCTGATTCTCCGTATCGTATGAAGCTGATCAAAGACAAAGGCTATGTGCACTTTTCAATCAATGGTCTGCCCATTCTTGAG  >  CP006881/732128‑732401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑