| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,725,237 | A→G | 100% | intergenic (‑69/‑460) | U712_19205 ← / → U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,725,237 | 0 | A | G | 81.3% | 90.2 / 0.7 | 45 | intergenic (‑69/‑460) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (26/10); minor base C (2/7); total (28/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
TTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CAG‑GCAGC‑C‑T‑TT‑T‑AGCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTA > CP006881/3725119‑3725362 | ttCATCAAAAAGACCATCCCCACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CAG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGCtt > 1:191822/1‑139 (MQ=255)ttCATCAAAAAGACCATCCACCCCCGTAAAGATACAAGCAAACGTTTCATAGATAATCATCCCTCCGCGTATTTTTGG‑CAT‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTTCTCCTACGCAGCCCGCAAGAAAAGCCGGCCGagt > 6:201568/1‑137 (MQ=255) atcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CAG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtt > 3:99100/1‑136 (MQ=255) caAAAAGACAATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CAC‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTTATCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttcttt > 1:141128/1‑139 (MQ=255) aaaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CAG‑GCAGC‑CTT‑TT‑T‑AGCCGCCCGTTTTCTCAGCCGCCGCCCGCAAGAAAAGACGGGCGAtttcttt > 4:151019/1‑136 (MQ=255) aCCATCCACACCCTTAAAGATACAAGCAAACGTTTCATATAAAAACAGCCCTCCGATTCTTTTTGG‑CCG‑GCAGCTT‑T‑TT‑T‑CGCAGCCGGTTTTCTCAGCCGCAGCCCGCAATAAAACACGGCCGATTTCTTTTCTCGCa < 5:198719/139‑1 (MQ=255) aTCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CAG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTTCTCAGCCGCCGCCCGCAAGAAAAGACGGCCGATTTCTTTTATCGCaaa > 7:413370/1‑138 (MQ=255) cacaCCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CAG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAg > 8:29745/1‑139 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CAG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTTCTCAGCCGCCGCCCGCAAGAAAAGACGGCCGATTTCTTTTATCGCAAACTAGCTGctc > 2:243097/1‑137 (MQ=255) acaTACAAGCAAACGTTCAAAACAAAAACAACCCACCGCTTATTTTTGG‑CCG‑GCACC‑C‑T‑TT‑TAACCACCCCGTTTTCGCCCCCCCAGCCCCCAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTa < 3:409649/134‑1 (MQ=255) acaTACAACCAAACGTTTAATATAAAAAAACCCCCCCGCTGATTTTTGG‑CTG‑GCAGCTT‑T‑TT‑T‑CGCCGCCGGTTTTCTCCGCCCCCCCCCGCAACAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTa < 3:152516/134‑1 (MQ=255) aGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CAG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa < 7:41452/139‑1 (MQ=255) aGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CAG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa < 5:90137/139‑1 (MQ=255) aGAAACAAGCAAACGTTTCATACAAAAACATCCCTCCGCTGCTTTTTGG‑CAG‑GCCGC‑CTT‑TT‑T‑AGCAGCCCTTTTTCTCAGCCGCAGCCCGCATTAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa < 6:349639/139‑1 (MQ=255) ctacaaaGAAAACGTTTCATACAAAAACATCCCTCCGCTTATTTTTGC‑CCG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTGCACAGCCGCCCCCCCCAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaa < 1:243097/134‑1 (MQ=255) aTACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CAG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTATCTCAGCCGCACCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaa > 8:13318/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CGG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaaa > 8:317978/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CAG‑GCAGC‑CGT‑TT‑T‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCGTTTCTCGCAAACTAGCGGCTCTAGCAAAAc > 8:332055/1‑139 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTCTTTTTGG‑CAG‑GCAGC‑CTT‑TT‑T‑AGAAGCCCGTTTTCTCAGCCGCAGCCCCCAAGAAAAGACGGCCGCTTTCTTTTCACGCAAAAAAGCGGCTCAAGCaaaa > 5:334611/1‑138 (MQ=255) aGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTATTTGG‑CAC‑GCAGC‑G‑TGTT‑T‑AGCACCCCGGTTTCTCATCCGCAGCCCCCAAGAAAAGACGGCCGCTTTCGTTTCACGCAAACAACGCGCTCTAGCaaacc > 4:136995/1‑134 (MQ=255) gCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CCG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTTCTCAGCCCCAGCCCGAAAGAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 7:249352/139‑1 (MQ=255) caaaaCGTTCCATACAAAACAATCCCCCCGCTTTTTTTTGG‑CCG‑GCAGC‑CTT‑TT‑T‑AGCCGCCCGTTTTCCCCGCCGCCCCCCCCAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 1:65486/137‑1 (MQ=255) cAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTTG‑CAG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTATCGGCACTAGCaaaaat > 4:371284/1‑133 (MQ=255) aaaCGTTTCAAAAAAAAACTTCCCCCCGCTTATTTTTGC‑CTG‑GCAGC‑CTT‑TT‑T‑ACCCGCCCTTTTTCTCCGCCCCACCCCGCAACAAACCACGGCCGATTTCTTTTCTCGAAAACTCGCGGCTCTAGCAAAACTCa < 4:212341/136‑1 (MQ=255) aaCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CAG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAc > 6:192692/1‑139 (MQ=255) cgttttCTTACAAAAACACCCCT‑CGCTTTTTTTTGGCCCG‑CCAGCTT‑T‑TT‑T‑CCCAGCCCGTTGTCCCCCCCCCCCCCCCCAATAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATg < 4:149152/133‑1 (MQ=255) acttttAATACAAAACCATCCCCCCGCTTTTTTTTGG‑CGG‑GCAGC‑CTT‑TT‑T‑CGCAGCCCGTTTTCTCCGCCCCACCCCGCAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 7:317978/136‑1 (MQ=255) actttacATAAAAAAACACCCCTCGCTTTCTTTTTGG‑CCGCCCGGT‑T‑T‑TT‑T‑CGCCGCCCTTTTTCTCCCCCCCCCCCCCCAACAAACCCCGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATg < 4:145132/130‑1 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CAG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct > 1:19484/1‑139 (MQ=255) gTTTCATATAAAAACAGCCCGCCGTTGATTTTTGG‑CCG‑GCCGC‑G‑T‑TTAT‑AGCCGCCCGTTTTCTCCGCCGCCGCCCGAACAAAAACACGGCCTATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAc < 4:331593/136‑1 (MQ=255) cATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CAG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTATTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACACTAtta > 8:181624/1‑137 (MQ=255) aaaCATCCCTCCGCTTCTTTTTGG‑ACG‑GCAGC‑CGT‑TT‑T‑AGCAGCCCGGTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTATAGCAAACTAGCGGCTATATCAAAACAAATTCCTAGATGATAGAACg > 6:225287/1‑139 (MQ=255) ccccccGATTTTTTTTGC‑CCG‑GCAGCTT‑T‑TT‑T‑AGCAGCCGGTTTTCCCAGCCCCCGCCCCAAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTgg < 3:371284/136‑1 (MQ=255) cGCTTCTTTTTGG‑CAG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGaa > 7:203556/1‑139 (MQ=255) atttttGG‑CCG‑GCAGC‑CTT‑TT‑T‑AGCAGCCGTTTTTCTCAGCCCCCGCCCGAAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCt < 8:203556/137‑1 (MQ=255) tttttGG‑CAG‑GCAGC‑CTT‑TT‑T‑AGCAGCCCGGTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTAGCAAACTAGCGGCGCGAGCAAAACACATGACTAGTTGATTGACCGTGGAAATAGaaata > 4:391721/1‑133 (MQ=255) tgcctt‑tt‑t‑CGCAGCCCGTGCTCTCCCCCGCAGCCCCAAAGAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGa < 7:13318/134‑1 (MQ=255) t‑t‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACGCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATTTGAGTGaaa > 8:211762/1‑138 (MQ=255) agcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAAcc > 8:11979/1‑138 (MQ=255) cGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTTTAACAATTTTAGTGAAAAAAcaca > 4:39958/1‑133 (MQ=255) gTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGATTGAAAAAACCaa > 3:10242/1‑135 (MQ=255) gTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAAACaa > 4:317559/1‑135 (MQ=255) cAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTGTTTTCTCGCCAACTAGCGGCTCGAGCAAAACTCATGACGCTATGATAGACCGTGGAAAAAGATTTGTTTGTAACCATATGAGTTAAAAAAACAACTAGattttt > 2:251957/1‑137 (MQ=255) cAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGtttt > 6:296280/1‑136 (MQ=255) cAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTATTTTCTAGCAAACTAGCGGCTCGAGCAAAACTCATGACTCGATGATAGCCCGTGGAAATATAATTATTTGTCCAAATATGAGTTGAAAAAACAAATAGtttttt > 2:381564/1‑137 (MQ=255) | TTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGG‑CAG‑GCAGC‑C‑T‑TT‑T‑AGCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTA > CP006881/3725119‑3725362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |