Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	52,896	T→C	48.7%	V45A (GTT→GCT)	U712_00225 →	Protein veg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	52,896	0	T	C	48.7%	‑5.1 / 112.9	76	V45A (GTT→GCT)	U712_00225	Protein veg
Reads supporting (aligned to +/- strand):  ref base T (15/24);  new base C (17/20);  total (32/44)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGT  >  CP006881/52759‑53031                                                                                                                                          |                                                                                                                                        tGCAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGtt                                                                                                                                        >  7:386694/1‑139 (MQ=255)    aaTGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGttttt                                                                                                                                     >  5:99339/1‑139 (MQ=255)     aTGGCGAAGACGGTGGCCGATATTAAAAGATCGCTAGATGGGACATTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTtg                                                                                                                                    <  8:409985/139‑1 (MQ=255)      tGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTtgt                                                                                                                                   >  1:165980/1‑139 (MQ=255)      tGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTtgt                                                                                                                                   >  1:93572/1‑139 (MQ=255)        gCGAAGACGTTGTCCGATTTTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGa                                                                                                                                 <  5:258441/139‑1 (MQ=255)        gCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTtg                                                                                                                                    >  4:264988/1‑136 (MQ=255)         cGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGAt                                                                                                                                <  6:343932/139‑1 (MQ=255)          gAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTtgtg                                                                                                                                  <  4:293426/136‑1 (MQ=255)            aGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAGGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGa                                                                                                                                 >  3:142246/1‑135 (MQ=255)              aCGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAACACGATTGAGCGTTCCGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACaa                                                                                                                            >  2:149786/1‑138 (MQ=255)              aCGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAAc                                                                                                                           >  5:61158/1‑139 (MQ=255)                  tGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAAc                                                                                                                           >  5:369848/1‑135 (MQ=255)                         tatTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAg                                                                                                                        <  1:231544/131‑1 (MQ=255)                         tatTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAg                                                                                                                        >  2:231544/1‑131 (MQ=255)                         tatTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGAc                                                                                                                >  7:329448/1‑139 (MQ=255)                           ttAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCGTCTGTGTTTGGGAGACAACTAGATCAAGa                                                                                                                 >  3:55277/1‑136 (MQ=255)                               aaGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGCTTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACgag                                                                                                             <  4:412181/136‑1 (MQ=255)                                 gATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGaa                                                                                                           >  4:149440/1‑136 (MQ=255)                                   tCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGt                                                                                                      >  7:352592/1‑139 (MQ=255)                                   tCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGt                                                                                                      >  5:350249/1‑139 (MQ=255)                                   tCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGt                                                                                                      >  2:236605/1‑139 (MQ=255)                                      cTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCATTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCga                                                                                                      >  3:24121/1‑135 (MQ=255)                                         gATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGaaa                                                                                                <  8:283438/139‑1 (MQ=255)                                          aTGTGAATTTAGGTAACAGGCTGACGTTAAAAGCAAACTGTGTCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTATCTGAGAAGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGaaa                                                                                                <  6:233044/138‑1 (MQ=255)                                          aTGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTg                                                                                                   <  3:417461/135‑1 (MQ=255)                                          aTGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTg                                                                                                   <  4:408998/135‑1 (MQ=255)                                          aTGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGaaa                                                                                                <  6:61158/138‑1 (MQ=255)                                              gAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGtt                                                                                           <  5:360179/139‑1 (MQ=255)                                               aaTTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGttt                                                                                          >  2:61063/1‑139 (MQ=255)                                                 tttAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCa                                                                                        <  5:21876/139‑1 (MQ=255)                                                 tttAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCa                                                                                        <  8:274737/139‑1 (MQ=255)                                                 tttAGGTAAAAGGCTGACGCTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCa                                                                                        <  7:186720/139‑1 (MQ=255)                                                    aGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATa                                                                                      <  1:9411/138‑1 (MQ=255)                                                         aaaGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGAt                                                                                                                                <  7:158916/91‑1 (MQ=255)                                                         aaaGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGAt                                                                                                                                >  8:158916/1‑91 (MQ=255)                                                             gCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTAt                                                                               <  3:163875/136‑1 (MQ=255)                                                                 aCGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCGTCTGTTTTTGTGATACAAATAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGata                                                                        >  7:213477/1‑139 (MQ=255)                                                                      aaaaGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATAtttt                                                                    >  6:90945/1‑138 (MQ=255)                                                                        caGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATAtttt                                                                    <  4:251581/135‑1 (MQ=255)                                                                        acgCAAACGGTGGCCGCCGAAAAACGATTGAGCGGTCGGGCATTATAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAGGAGTTTCATACAGTTATGCTGATAtttt                                                                    <  4:272187/134‑1 (MQ=255)                                                                        aaGCAACCGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGATGAGACGTACCCTTCGGTGTTTGCGTTACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATAAAGTTATGCTGATAtttt                                                                    <  4:262840/136‑1 (MQ=255)                                                                        aaGCAAACGGTGGCCTCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGAc                                                                 <  5:187775/139‑1 (MQ=255)                                                                        aaGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGAc                                                                 <  8:386694/139‑1 (MQ=255)                                                                          gCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGTTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGa                                                                  >  3:12477/1‑136 (MQ=255)                                                                           cacaCGGTGTCCGCCGAAAAACGATTGAGCGGTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTga                                                              <  2:214576/136‑1 (MQ=255)                                                                           cAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTga                                                              <  7:306533/139‑1 (MQ=255)                                                                                ggTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTgaga                                                            <  3:163445/136‑1 (MQ=255)                                                                                   ggCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTg                                                      <  8:230097/139‑1 (MQ=255)                                                                                   ggCCGCCGAAAAACGAGTGCGCGTTCGGGCATTTTAGCGGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTg                                                      <  6:56384/139‑1 (MQ=255)                                                                                     ccgccgATATACGATTGTGAGTTGGGTCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGATCTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGtt                                                       <  4:24121/136‑1 (MQ=255)                                                                                     ccgccgAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGtt                                                       <  4:142246/136‑1 (MQ=255)                                                                                       gccgAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCt                                                  <  8:329448/139‑1 (MQ=255)                                                                                       gccgAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCt                                                  <  8:352592/139‑1 (MQ=255)                                                                                       gccgAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGa                                                     <  3:298655/136‑1 (MQ=255)                                                                                       gccgAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGa                                                     <  3:292834/136‑1 (MQ=255)                                                                                       gccgAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCt                                                  <  1:236605/139‑1 (MQ=255)                                                                                                      aGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAAtgatga                                   >  8:154898/1‑139 (MQ=255)                                                                                                            cGGGCATTTTCGCTGAGACGTACCCTTCTGTTTTGGTGATCCAACTAGATCAAGACGCGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCGGACATTTAATGATGAcgccgc                             <  8:209517/139‑1 (MQ=255)                                                                                                            cGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGAcgccgc                             <  7:154898/139‑1 (MQ=255)                                                                                                             gggAATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGAcgcc                               <  4:423072/136‑1 (MQ=255)                                                                                                                           agaCGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATa                                                                                      >  1:164993/1‑67 (MQ=255)                                                                                                                           agaCGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATa                                                                                      <  2:164993/67‑1 (MQ=255)                                                                                                                                tACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCAtttt            <  3:149440/136‑1 (MQ=255)                                                                                                                                  cccTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACg        <  1:61063/138‑1 (MQ=255)                                                                                                                                   ccTTCTGTTTTTGTGATACAACTAGATCAAGACGACAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACggg      <  6:369848/139‑1 (MQ=255)                                                                                                                                   ccTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACggg      <  1:369843/139‑1 (MQ=255)                                                                                                                                    cTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACg        >  4:205604/1‑136 (MQ=255)                                                                                                                                     ttCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACggg      >  6:190552/1‑137 (MQ=255)                                                                                                                                     ttCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACggg      >  5:217054/1‑137 (MQ=255)                                                                                                                                     ttCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACggg      >  2:369843/1‑137 (MQ=255)                                                                                                                                     ttCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACggg      >  1:68837/1‑137 (MQ=255)                                                                                                                                       cTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGCCATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGt  >  2:284985/1‑139 (MQ=255)                                                                                                                                       cTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGt  >  5:370258/1‑139 (MQ=255)                                                                                                                                       cTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTCAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGt  >  2:267070/1‑139 (MQ=255)                                                                                                                                       cTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGt  >  6:119423/1‑139 (MQ=255)                                                                                                                                       cTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGt  >  5:133868/1‑139 (MQ=255)                                                                                                                                       atGTTTTTGTTATACAAGTAGTTCAAGACGATTAATGGTTTGAATGTTTTTCTTACAGTTATGCTGATATTTTGACTGAGACTTTTGTGCTTACATTTAATTATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAg   <  6:133868/137‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TGCAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGT  >  CP006881/52759‑53031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑