BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,735	A→T	9.6%	intergenic (+20/‑136)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,735	0	A	T	9.6%	13966.8 / inf	4891	intergenic (+20/‑136)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (263/4107); new base T (498/22); total (761/4130)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4996 total aligned reads displayed.

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  Exported/3597‑3877

 tAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTa                                                                                                                                                  >  2:311212/1‑138 (MQ=255)

      aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTT‑AAt                                                                                                                                              >  3:290172/1‑136 (MQ=255)

        cAGTTTCATCAAGCAATCAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGa                                                                                                                                         <  7:179525/139‑1 (MQ=255)

         aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTACCGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑Atttt                                                                                                                                            <  4:75728/135‑1 (MQ=255)

         aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGaa                                                                                                                                        <  5:342889/139‑1 (MQ=255)

         aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGa                                                                                                                                         <  1:240889/138‑1 (MQ=255)

           tttCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACAATTACTAATGAGCAAGTATTGTCTATTTTTAATAGTTATATATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTATAAtttttaa                                                                                                                                        >  8:325287/1‑134 (MQ=255)

              catcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAg                                                                                                                                      <  4:256330/136‑1 (MQ=255)

               atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTa                                                                                                                                  <  7:128582/138‑1 (MQ=255)

                tgaaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTaca                                                                                                                                <  8:299086/137‑1 (MQ=255)

                tcaAGCATTGAAACTCGCCAAAGTAAACAATTTAAGTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTTTTATTTAACGGGAGGAAATAATTCTATTAGTCGC‑T‑TTTTTA‑AATTTTGAAAT‑TTaca                                                                                                                                <  6:338346/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑tt                                                                                                                                   <  4:105839/136‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑tt                                                                                                                                   <  3:108384/136‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑tt                                                                                                                                   <  3:196438/136‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑tt                                                                                                                                   <  4:291042/136‑1 (MQ=255)

                taaatcAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATATTTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTaca                                                                                                                                <  2:233420/134‑1 (MQ=255)

                 caAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTacac                                                                                                                               <  5:313102/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTac                                                                                                                                 <  3:48876/136‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACg                                                                                                                              <  1:42328/139‑1 (MQ=255)

                  aaGCAAAGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATAAGTCGC‑T‑TTTTTT‑AATTTGGAAAG‑TTACACg                                                                                                                              <  8:383450/139‑1 (MQ=255)

                       aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACt                                                                                                                         <  6:362904/139‑1 (MQ=255)

                       aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACt                                                                                                                         <  8:73749/139‑1 (MQ=255)

                          aaaCACGACAAAGTAAACAAATTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTaaa                                                                                                                      <  8:259651/139‑1 (MQ=255)

                           aaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTaaa                                                                                                                      <  6:304761/138‑1 (MQ=255)

                               cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTTCACGTTACTAAAGGGaa                                                                                                                 <  2:423326/139‑1 (MQ=255)

                               cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAgg                                                                                                                    <  3:167815/136‑1 (MQ=255)

                               cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGaa                                                                                                                 <  8:10117/139‑1 (MQ=255)

                               cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGaa                                                                                                                 <  8:42198/139‑1 (MQ=255)

                                gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAAt                                                                                                                <  7:229339/139‑1 (MQ=255)

                                     aGTAAACAATTTAAGTACCAATACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGgaga                                                                                                           <  2:198465/139‑1 (MQ=255)

                                      gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGga                                                                                                             <  4:392506/136‑1 (MQ=255)

                                      gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGAt                                                                                                          <  7:148213/139‑1 (MQ=255)

                                      gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGAt                                                                                                          <  1:172675/139‑1 (MQ=255)

                                          aCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGATATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGTAAG‑TTTCACGGTTCTTAAGGGTATGGGGatatat                                                                                                      >  2:272616/1‑139 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTT‑TATTTTGTTAG‑TTTCAAGTTTATAAAGTGTATGGGTATTAattatt                                                                                                  >  7:285155/1‑139 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  5:1968/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  5:139735/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  2:385088/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  1:126723/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAatt                                                                                                     <  3:7601/136‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAatt                                                                                                     <  4:357889/136‑1 (MQ=255)

                                                 aaGTTCCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTAATAAAGGGAATGGAGATAAATTATTAg                                                                                                <  1:207675/138‑1 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAattat                                                                                                   <  3:405274/135‑1 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTT‑AATTTTGTAAG‑GTTCACGTTACTAAAAGGAATGGAGATAAAttattata                                                                                               >  8:131330/1‑137 (MQ=255)

                                                   gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                             <  1:9532/139‑1 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                           <  1:367439/138‑1 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                           <  1:296397/138‑1 (MQ=255)

                                                      ccATTACTTATGAGCAAGTAATGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                           <  5:168809/138‑1 (MQ=255)

                                                         ttACTTATGAGCAAGTATTGTCTATTTTTAATATTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATAtac                                                                                          <  4:44482/136‑1 (MQ=255)

                                                            catATGAGCAAGGATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTAGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                    <  2:136010/137‑1 (MQ=255)

                                                            cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACTGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATAtactac                                                                                       <  4:32754/136‑1 (MQ=255)

                                                            cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                    <  6:230593/139‑1 (MQ=255)

                                                             gacagAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                   <  7:366644/135‑1 (MQ=255)

                                                               aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                   <  6:105373/137‑1 (MQ=255)

                                                                 gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTT‑AATTTTGAAAT‑TTTAACGTTAATAAAGGGAATGGTGATAAAATATTATATATAATAATGACAGCt                                                                               >  6:199201/1‑139 (MQ=255)

                                                                    cAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTT‑TATTTGGGTAGTTTGCAGGTT‑GTTAATGGAATGGTGATATATTTTTTGTTTTTCTACTGGCAGcg                                                                               >  4:215841/1‑135 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCtt                                                                              <  3:260984/136‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  8:347448/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  5:250598/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  1:320131/139‑1 (MQ=255)

                                                                      aGTATTGTCTATTATTAATACTAATCTATTATTTATCGCGAGGAAATAATTCTATGCGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAAAGCTTCCa                                                                           <  6:385826/138‑1 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTcc                                                                            <  3:348792/136‑1 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                         <  7:20493/139‑1 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  7:395129/137‑1 (MQ=255)

                                                                            gTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGc                                                                     <  8:19945/138‑1 (MQ=255)

                                                                            gTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGc                                                                     <  1:274117/138‑1 (MQ=255)

                                                                             tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                      <  3:3774/136‑1 (MQ=255)

                                                                               tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                 <  5:224172/139‑1 (MQ=255)

                                                                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TGTTTTTA‑ATTTTGTAAAT‑TTAAACGTT‑CTATAAGGAAAGGGGATAAATTTTTATATATAAAAATAAAAGCTTACAAAAAGCAAAagag                                                              >  2:167876/1‑139 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  5:110830/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  1:149703/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGACGAAATAATTCTATGAGTCGG‑T‑TTTTTA‑TATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTCGATATACTCCTGACAGCTGCCATGAAGCTAAcgag                                                              <  5:45601/138‑1 (MQ=255)

                                                                                        tAGTTATCTATTATTTAAAGGTAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCt                                                        <  8:232616/139‑1 (MQ=255)

                                                                                          tttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTAGC‑T‑TTTTTA‑ATTTTGTAAAG‑TTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTa                                                       <  6:136049/137‑1 (MQ=255)

                                                                                           ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTA‑TTATTTTTAAG‑TTTGACGGTAAAAAAGGGAATGGAGATAAATTATTTTAAATACTACTGAAACCTTACAAGAAACAAAAGATGTCCCGAgc                                                     >  6:77478/1‑139 (MQ=255)

                                                                                             aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGT‑TTTTTA‑TTTTTTTTAAT‑TTA‑ACGTTAATAAAGGGGATTGAGAATAATTATTATATTTTATACAGACACCGTACAAAAAGGTAAATAGGTGCCGAgccc                                                   >  2:71160/1‑137 (MQ=255)

                                                                                                        aaCGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGaa                                         <  7:74897/138‑1 (MQ=255)

                                                                                                           gggAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAACG‑TTACACTTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTG                                     <  1:344602‑M1/139‑1 (MQ=255)

                                                                                                             gaggaAATAATTCTATGAGTCGCGT‑TTTTTA‑ATTTTGTAAAT‑TTA‑AAGGTACTAAAGGGTATTGAGATTAAATTTTATATATAATAAAGACAGCGTCAAAGAAGCTAAATAGGTCCCGAGCGCCAACGGGGAAttt                                      >  4:180948/1‑136 (MQ=255)

                                                                                                             gaggaAATAATTCTATGAGTCGCGG‑TTTTTA‑ATTTTGTAAGG‑TTA‑ACGGTAATTAAAGGAATTGTGGTAAAATATTATATATAATACTGACAGCATCACAGAAGCGAAAAAGGTACCCAGAGCCGAACGGGCAttttgg                                   >  8:15784/1‑136 (MQ=255)

                                                                                                                      atTGTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACTTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACATCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGTAATTTGgggtacattga                          <  5:374581‑M1/139‑12 (MQ=255)

                                                                                                                         cTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                        <  1:393836‑M1/138‑14 (MQ=255)

                                                                                                                            tGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  7:258392‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  1:69534‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  7:336664‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  1:101217‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  7:47555‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                       <  3:32030‑M1/136‑15 (MQ=255)

                                                                                                                            tGAGTAGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  5:407671‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGGCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                       <  4:138528‑M1/136‑15 (MQ=255)

                                                                                                                             gAGTCGC‑T‑TTTTTA‑AATTTGTAAAG‑TTACACTTTACTAAAGGGAATGGAGATAAATTTTTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  6:166565‑M1/138‑18 (MQ=255)

                                                                                                                             gAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  7:312235‑M1/138‑18 (MQ=255)

                                                                                                                              attatc‑T‑TTTTTA‑AATTTGGATAG‑TTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGAAAGCTTCCAAGAAGCTAACGAGGTCCCTATCGCCTACGGGTAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGaa             <  6:426852/134‑1 (MQ=255)

                                                                                                                              aGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaa                      <  3:133158‑M1/135‑16 (MQ=255)

                                                                                                                               gTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  7:108937‑M1/138‑20 (MQ=255)

                                                                                                                                       tttttA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGATATAAATTATTATATATAATACTTACAGCTTCAAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGTGAATTTGgggtacattgaaaaaggaagagtat            <  5:279476‑M1/138‑26 (MQ=255)

                                                                                                                                        ttttA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagag               <  3:227907‑M1/134‑23 (MQ=255)

                                                                                                                                            a‑aaTTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAATGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta       <  2:347997‑M1/138‑31 (MQ=255)

                                                                                                                                            a‑aaTTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta       <  5:77034‑M1/138‑31 (MQ=255)

                                                                                                                                            a‑aaTTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta       <  6:263221‑M1/138‑31 (MQ=255)

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTA‑AATTTGGAAAG‑TTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  Exported/3597‑3877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`