BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,749	A→T	5.6%	intergenic (+34/‑122)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,749	0	A	T	5.6%	13433.4 / 113.9	4239	intergenic (+34/‑122)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (364/3561); new base T (286/13); total (658/3581)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4667 total aligned reads displayed.

CATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATT  >  Exported/3611‑3886

 atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTAGTTATGTGAAAGTATTTTATATTTTTAATATTTATATTTTTTTTAACGGTAGT‑AATTAATTGTTTGTGAGGATTTTTGAAATTTGGAAAGTTa                                                                                                                                           >  7:12361/1‑138 (MQ=255)

  tcatcaaaTGAAACACGCCAAAGTAAACAAATTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                         <  2:104171/133‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGGAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                         <  2:238365/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                         <  6:229646/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                         <  2:323410/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                         <  6:139357/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                         <  6:198462/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                         <  6:359258/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                         <  7:243209/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                         <  7:377274/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                         <  8:346939/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                         <  1:1258/139‑1 (MQ=255)

   caAGCAAAGAAACACGCCAAACTAAACAATTTAAGTACCATTACATATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTacac                                                                                                                                        <  1:379570/139‑1 (MQ=255)

    caGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTac                                                                                                                                          <  3:283214/135‑1 (MQ=255)

    aaGCAATGAAACACTCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                       <  6:261814/139‑1 (MQ=255)

         attaaaCACGACAAAGTAAACAATTTAAGTAAAATTACTTATGAGCAAGTTTTGTCTATTTTTAATATTTATCTATTATTTAACGGGAGT‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                                  <  8:203482/136‑1 (MQ=255)

         aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                                  <  8:73749/139‑1 (MQ=255)

         aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                                  <  5:350964/139‑1 (MQ=255)

                  gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAAATAATTCTATGAGTCGCGTTTTTTAATTTTGTTATTTAGACGGTACATAAgggta                                                                                                                          >  5:83445/1‑137 (MQ=255)

                  gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGt                                                                                                                                      >  2:84996/1‑126 (MQ=255)

                       aGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTTAATTTTTTAATTTAAAAGTTAATAAAAGGAAttg                                                                                                                       >  4:327991/1‑134 (MQ=255)

                        gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                                   <  5:322828/139‑1 (MQ=255)

                         tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                  <  6:68030/139‑1 (MQ=255)

                          aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGATG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                  <  7:56497/138‑1 (MQ=255)

                          aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                  <  2:356943/138‑1 (MQ=255)

                          aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                  <  2:204329/138‑1 (MQ=255)

                           aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                                <  2:284570/139‑1 (MQ=255)

                               aTTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAat                                                                                                               <  3:118282/136‑1 (MQ=255)

                                tttAAGTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAAAGTGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                           <  8:160204/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                           <  5:204241/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                           <  2:143203/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                           <  7:34917/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAatt                                                                                                              <  3:408327/136‑1 (MQ=255)

                                tttAACTACCAATACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAAATCTATGAGTCGCTTTTTTTAATTTGGAAAGTTACACGTTACAAAAGCCAATGGAGATAAatt                                                                                                              <  3:377972/136‑1 (MQ=255)

                                    aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAAttatta                                                                                                          <  3:215315/136‑1 (MQ=255)

                                    aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGa                                                                                                        <  7:168541/138‑1 (MQ=255)

                                     gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                      <  7:299923/139‑1 (MQ=255)

                                     gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                      <  6:383666/139‑1 (MQ=255)

                                        ccATTACTTATGAGCTAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                    <  2:173781/138‑1 (MQ=255)

                                        ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTATATTTGGAAAGTTAAACGTTTATTAAGGGAATGGAGATTAATTATTTTatattc                                                                                                   >  5:385374/1‑137 (MQ=255)

                                           ttACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTATATTTTTAAATTTTAACGTTACTAAAGGGAATGGTTATAAATTATTATATTtaata                                                                                                 >  6:300688/1‑138 (MQ=255)

                                           ttACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATAtactac                                                                                                <  5:146001/139‑1 (MQ=255)

                                              cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATAtactac                                                                                                <  4:24796/136‑1 (MQ=255)

                                              cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                             <  5:122652/139‑1 (MQ=255)

                                              cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCGTTTTTTAATTTTGAAACTTAAAACGTAAGAAAAGGAATTGTGATTAATTATTATATataata                                                                                                 >  3:145879/1‑135 (MQ=255)

                                                tATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCGGTTTTTAATTTTTTAATTTTCACGTTAAGAAATGGTATGGTGATAAATTATTATATATAATAATGAc                                                                                            >  1:228467/1‑138 (MQ=255)

                                                  tGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGc                                                                                         <  7:81967/139‑1 (MQ=255)

                                                       aaGTATTGTCTATTTTTTATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCTCTTTTTTAAATTTTGAAAGTTAAACGTTACTAACGGGAATGGAGATAATTTATTAGATATACTACTGACAGCTTCCa                                                                                    <  5:258597/139‑1 (MQ=255)

                                                       aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCtt                                                                                       <  4:48772/136‑1 (MQ=255)

                                                       aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCtt                                                                                       <  4:112820/136‑1 (MQ=255)

                                                         gTATTGTCTATTTTTAATAGTTATCTATTATTTATCGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                  <  1:76314/139‑1 (MQ=255)

                                                         gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTcc                                                                                     <  3:345686/136‑1 (MQ=255)

                                                         gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                  <  5:412380/139‑1 (MQ=255)

                                                         gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                  <  8:382646/139‑1 (MQ=255)

                                                          tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                 <  5:5292/139‑1 (MQ=255)

                                                          tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                    <  3:83194/136‑1 (MQ=255)

                                                               tCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCGTTTTTTAATTTTTTAATTTTAACGTTTATAAAAGGGATGGTGATAAAATATTATATTTAATAAAGAAACCTTACAAGAAGCt                                                                             >  2:47705/1‑138 (MQ=255)

                                                                    ttttAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAaga                                                                        <  2:395087/138‑1 (MQ=255)

                                                                    ttttAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCGTTTTTTAATTTTGAAATTTTAACGTTAATAAAGTGAATGGATATAAAATATTATATATAATACTGACACCTTCCAAAAAGCTaaaaac                                                                       >  6:422386/1‑136 (MQ=255)

                                                                     tttAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                       <  8:237140/138‑1 (MQ=255)

                                                                     tttAATAGTTATCTATTAATTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                       <  1:331248/138‑1 (MQ=255)

                                                                               aTCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAAATTTGAAAATTTAAAGTTACTAAATGGAAAGGATATAAAATATTAATAATAATAAATAAACCTTCCAAAAATCTAAAAAGGTCACTAgcgc                                                            >  6:112414/1‑139 (MQ=255)

                                                                               aTCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCGTTTTTAAATTTTTGAAGTTACAAGTTACTAAAGGGAATGGAGATAAATTTTTAAATATTATACTAAAAGACTGCAAGAAGCTAAAAAGGTCACTAccgc                                                            >  8:421821/1‑139 (MQ=255)

                                                                                  tattatTTAACGGGAGG‑AAATAATTCTATGAGTCGCGTTTTTTTATTTTTAAAGTTTAACGTTAATAAATGGAATGGTGATAAATTTTTTGATATAATACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCGAgcgc                                                            >  4:159128/1‑136 (MQ=255)

                                                                                      attTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACTCGTTACTAAAGGGAATGGAGATAAATTATTTTATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                      <  7:104311/138‑1 (MQ=255)

                                                                                       tttAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                     <  7:197131/138‑1 (MQ=255)

                                                                                            cGGGAGG‑AAATAATTCTATGAGTCGCGTTTTTTAATTTGGAAATTTACACGTTACTAAAGGGAATGGAGATAAATTATTATATATAATAATTACAGCTTCCAAACAGCTAAATAGTTCCCTAGCGCCTACGGGGAAttt                                               >  7:321054/1‑139 (MQ=255)

                                                                                              ggagg‑aAATAATTCTATGAGTCGCTTTTTTTAATTTTGAAATTTAAACGTTAATAAATGGTATTGTGATAAATTATTTTATATAATACTGACAGCTTCCAAGAAGCTAAATAGGTCCCTAGCGCCTACGGGGAAttttg                                             >  7:14374/1‑137 (MQ=255)

                                                                                               gagg‑aAATACTTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                            <  1:46949‑M1/139‑3 (MQ=255)

                                                                                                agg‑aAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                           <  8:325018‑M1/139‑4 (MQ=255)

                                                                                                 gg‑aAATAATTCTATTAGTCTATTTTTTTTATTTTTAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGTGGTCCCTAGCGCCTACGGGGAATTTGggg                                           <  8:299315‑M1/138‑4 (MQ=255)

                                                                                                 gg‑aAATAATTCTATGAGTCGCGGTTTTTTATTTTGTAATTTTCACGGTAGTTAAGGGTTTGGTGATTAATTTTTTTATATTAAACTGACAGCATGCAAAAAGCTAAAGAGGTGCCGATCGCCAACCGGGGATTTgg                                             >  4:83668/1‑135 (MQ=255)

                                                                                                         atTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                                   <  7:267747‑M1/139‑12 (MQ=255)

                                                                                                               tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                             <  1:101217‑M1/139‑18 (MQ=255)

                                                                                                               tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                             <  1:16940‑M1/139‑18 (MQ=255)

                                                                                                               tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                             <  1:352685‑M1/139‑18 (MQ=255)

                                                                                                               tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                                <  3:242662‑M1/136‑15 (MQ=255)

                                                                                                               tGAGTCGATTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                                <  3:315766‑M1/136‑15 (MQ=255)

                                                                                                                gAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                             <  1:306953‑M1/138‑18 (MQ=255)

                                                                                                                gAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                                <  4:285558‑M1/135‑15 (MQ=255)

                                                                                                                 aGTCTCGTTTTTAAATTTGGAAAGTAACCCGTTACTACAGGTCATGGACATAAATTATTAGATATACTACTGCCAGCTTCCAAGATGCTATAGCGGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaag                              <  4:401718‑M1/136‑17 (MQ=255)

                                                                                                                 aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaag                              <  3:53266‑M1/136‑17 (MQ=255)

                                                                                                                     gCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                           <  4:359758‑M1/135‑20 (MQ=255)

                                                                                                                       ttttttAAATTTGTAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                     <  8:108796‑M1/139‑26 (MQ=255)

                                                                                                                        tttttAAATTTGGAAAGTTACACGTTACTAATTGGAATGGAGATAATTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                     <  1:287012‑M1/138‑26 (MQ=255)

                                                                                                                        tttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATTGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                     <  1:407022‑M1/138‑26 (MQ=255)

                                                                                                                        tttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                     <  1:201804‑M1/138‑26 (MQ=255)

                                                                                                                         ttttAAATTTGTAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATATAATACTGACAGCTTCCAAGAAGCTAATGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                     <  5:230212‑M1/137‑26 (MQ=255)

                                                                                                                         ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                     <  1:26059‑M1/137‑26 (MQ=255)

                                                                                                                         ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                     <  5:173430‑M1/137‑26 (MQ=255)

                                                                                                                         ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                     <  8:262692‑M1/137‑26 (MQ=255)

                                                                                                                         ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagag                        <  3:180948‑M1/134‑23 (MQ=255)

                                                                                                                          tttAAATTTGGAAAGTTACACGTTACTAAAGGGAATTGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCTTACGGGGAATTTGgggtacattgaaaaaggaagagtatga                   <  7:408538‑M1/138‑28 (MQ=255)

                                                                                                                          tttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatga                   <  6:336943‑M1/138‑28 (MQ=255)

                                                                                                                          tttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatga                   <  5:293804‑M1/138‑28 (MQ=255)

                                                                                                                             aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                <  6:168680‑M1/138‑31 (MQ=255)

                                                                                                                             aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                <  7:218144‑M1/138‑31 (MQ=255)

                                                                                                                             aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                <  1:147584‑M1/138‑31 (MQ=255)

                                                                                                                             aaaTTTGGAAAGATACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatga                   <  4:199704‑M1/135‑28 (MQ=255)

                                                                                                                              aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt              <  8:138990‑M1/139‑33 (MQ=255)

CATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGG‑AAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATT  >  Exported/3611‑3886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`