Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 999,467 G→C 5.3% V294L (GTC→CTC)  U712_04880 → UPF0053 protein yhdT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881999,4670GC 5.3% 272.3 / 5.6 76V294L (GTC→CTC) U712_04880UPF0053 protein yhdT
Reads supporting (aligned to +/- strand):  ref base G (34/37);  new base C (2/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

CTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACTTGTGACGACAGAGGATATTCTTGA  >  CP006881/999337‑999600
                                                                                                                                  |                                                                                                                                     
cTGGCCAGTCACAGATAGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGaa                                                                                                                               <  1:228406/139‑1 (MQ=255)
        tCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCgg                                                                                                                                       >  7:327412/1‑123 (MQ=255)
        tCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCgg                                                                                                                                       <  8:327412/123‑1 (MQ=255)
             gATGGAGACAAAGACAGTGAGCTCGGTTTGGTAAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACg                                                                                                                   >  1:80426/1‑138 (MQ=255)
                caggaCAACGAAAGTGTGCTCGTTTTGGTCAATACTAAGCATTTGGTTTCTGATTTGATGTTTATGACAGAAGAACAACGAATGACCATGACGATCCATCCGTATGGGAGGCCGGTCATCGAAGTGATTGAAACGAtac                                                                                                               <  6:2505/136‑3 (MQ=255)
                 gagaCAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATcc                                                                                                               <  5:410707/138‑1 (MQ=255)
                  agaCAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCg                                                                                                             >  8:166881/1‑139 (MQ=255)
                              tgtgCTCGGTTTGGTCAATACTAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGCCGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTAATGACCTg                                                                                                 >  5:2505/1‑139 (MQ=255)
                                tgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACtt                                                                                                  <  4:278654/136‑1 (MQ=255)
                                tgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTg                                                                                                 <  5:319984/137‑1 (MQ=255)
                                tgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTg                                                                                                 >  6:319984/1‑137 (MQ=255)
                                 gCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTg                                                                                                 >  3:420652/1‑136 (MQ=255)
                                 gCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTg                                                                                                 >  3:16534/1‑136 (MQ=255)
                                   tCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATTAAGATGACGATCAATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCTGTTCATGACTTGCt                                                                                               >  3:312506/1‑136 (MQ=255)
                                   tCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCt                                                                                               >  3:101137/1‑136 (MQ=255)
                                      gTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGAt                                                                                            >  4:325755/1‑136 (MQ=255)
                                                  cGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAgcg                                                                                >  3:274882/1‑136 (MQ=255)
                                                  cGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGa                                                                             <  7:166881/139‑1 (MQ=255)
                                                   gAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAg                                                                            <  2:249076/139‑1 (MQ=255)
                                                   gAAGCATTTGTTTTCATATTTTATGTTTATGACAGAAGAAGAAAGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAg                                                                            <  1:152379/139‑1 (MQ=255)
                                                     aGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAAc                                                                                                                    >  2:60753/1‑97 (MQ=255)
                                                     aGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAAc                                                                                                                    <  1:60753/97‑1 (MQ=255)
                                                      gCATTTGTTTTCTGATTTGCTGATTCTGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAg                                                                            <  4:162178/136‑1 (MQ=255)
                                                       aaTTTGTTTTCTGATTTGCTGTTTATGACAGTAGAAGAACGAATGAAGATGACGATGCATCCGTATGTGCGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCa                                                                        <  6:213360/138‑1 (MQ=255)
                                                        aTTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATc                                                                                                                                  <  3:188589/80‑1 (MQ=255)
                                                        aTTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATc                                                                                                                                  >  4:188589/1‑80 (MQ=255)
                                                        aTTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAGACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAgcg                                                                          >  4:71283/1‑136 (MQ=255)
                                                          ttGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCAtt                                                                      >  7:396812/1‑138 (MQ=255)
                                                          ttGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCAtt                                                                      >  8:53419/1‑138 (MQ=255)
                                                          ttGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCAtt                                                                      >  5:186961/1‑138 (MQ=255)
                                                          ttGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCAtt                                                                      >  1:135056/1‑138 (MQ=255)
                                                          ttGATTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGTTCATCGAAGTGATTTAAACTATCCCGTTTCATGACTTGCTGATTAAAATGAAGCGCTATCTCAtt                                                                      >  6:251106/1‑138 (MQ=255)
                                                            gTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCAt                                                                       <  4:101137/135‑1 (MQ=255)
                                                             ttttGTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTc                                                                     <  4:369952/136‑1 (MQ=255)
                                                             ttttCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAgc                                                                           <  3:47045/130‑1 (MQ=255)
                                                             ttttCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAgc                                                                           >  4:47045/1‑130 (MQ=255)
                                                             ttttCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCAtt                                                                      >  4:68717/1‑135 (MQ=255)
                                                             ttttCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTc                                                                     <  3:184062/136‑1 (MQ=255)
                                                               ttCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCa                                                                    <  4:274882/135‑1 (MQ=255)
                                                                 cTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCata                                                                  <  4:172701/135‑1 (MQ=255)
                                                                            tttATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCg                                                    <  2:135056/138‑1 (MQ=255)
                                                                            tttATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCAGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCg                                                    <  8:396812/138‑1 (MQ=255)
                                                                               aTGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCg                                                    <  4:420652/135‑1 (MQ=255)
                                                                               aTGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGAt                                                 <  6:186961/138‑1 (MQ=255)
                                                                                    agaagaagaACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATAc                                           >  8:133110/1‑139 (MQ=255)
                                                                                      aagaagaaCGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCt                                                                                               >  6:385349/1‑85 (MQ=255)
                                                                                      aagaagaaCGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCt                                                                                               <  5:385349/85‑1 (MQ=255)
                                                                                      aagaagaaCGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAgcgc                                                                         >  8:7727/1‑107 (MQ=255)
                                                                                      aagaagaaCGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAgcgc                                                                         <  7:7727/107‑1 (MQ=255)
                                                                                      aagaagaaCGAATGAAGATGACGATCCATCCGTATGTGAGGCCGCTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCt                                                                                               <  5:267848/85‑1 (MQ=255)
                                                                                      aagaagaaCGAATGAAGATGACGATCCATCCGTATGTGAGGCCGCTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCt                                                                                               <  5:267844/85‑1 (MQ=255)
                                                                                      aagaagaaCGAATGAAGATGACGATCCATCCGTATGTGAGGCCGCTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCt                                                                                               >  6:267848/1‑85 (MQ=255)
                                                                                      aagaagaaCGAATGAAGATGACGATCCATCCGTATGTGAGGCCGCTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCt                                                                                               >  6:267844/1‑85 (MQ=255)
                                                                                       agaagaaCGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATAc                                           >  4:215926/1‑136 (MQ=255)
                                                                                         aagaaCGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACgg                                         >  4:378480/1‑136 (MQ=255)
                                                                                              cGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCt                                 >  6:351443/1‑139 (MQ=255)
                                                                                                aaTGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCg                               >  6:105576/1‑139 (MQ=255)
                                                                                                  tGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCggg                             <  5:209429/139‑1 (MQ=255)
                                                                                                    aaGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCg                               <  3:325755/135‑1 (MQ=255)
                                                                                                      gATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACtt                         >  2:9444/1‑139 (MQ=255)
                                                                                                          aCGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACTTGTga                     >  6:205197/1‑139 (MQ=255)
                                                                                                               ccatccGTATGTGAGGCCGGTCATCGAAGTGATTGAGACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACTTGTgacg                   <  3:71283/136‑1 (MQ=255)
                                                                                                               agatccGTATGTGAGGATGGTCATCTAAGTTTTTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCATCGCGAGCGAATTAATATGGCTATATTGACGGATGAATCCGGAGGAACCTCGGGACTTGTGACGACa                <  2:383174/137‑1 (MQ=255)
                                                                                                                catccGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACTTGTgacg                   <  3:215926/135‑1 (MQ=255)
                                                                                                                catccGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACTTGTGACGACa                <  7:133110/138‑1 (MQ=255)
                                                                                                                catccGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACTTGTGACGACa                <  5:105576/138‑1 (MQ=255)
                                                                                                                 atccGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACTTGTGACGACaga              <  5:251106/139‑1 (MQ=255)
                                                                                                                    cGTATGTGAGGCCGGTCATCGATTTGATTTAAACGATCCCGGTTAATGAATTGATGATTAAAATGCAGCGCGAGGGCATTCATATGGCTATATTGTCTGATGAATACGGAGGAACCTCGGGACTTGTGACGACaga              <  4:312506/136‑1 (MQ=255)
                                                                                                                        tgtgAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACTTGTGACGACAGAGGatat        >  2:354162/1‑138 (MQ=255)
                                                                                                                         gtgAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAgc                                                                                 >  4:203991/1‑64 (MQ=255)
                                                                                                                         gtgAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAgc                                                                                 <  3:203991/64‑1 (MQ=255)
                                                                                                                            aGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACTTGTGACGACAGAGGATATTCtt    >  1:242029/1‑138 (MQ=255)
                                                                                                                            aGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACTTGTGACGACAGAGGATATTCtct   >  1:383174/1‑137 (MQ=255)
                                                                                                                              gCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACTTGTga                     >  6:367286/1‑119 (MQ=255)
                                                                                                                              gCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACTTGTga                     <  5:367286/119‑1 (MQ=255)
                                                                                                                               ccGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACTTGTgacg                   >  3:333465/1‑120 (MQ=255)
                                                                                                                               ccGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACTTGTgacg                   <  4:333465/120‑1 (MQ=255)
                                                                                                                                cGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACTTGTGACGACAGAGGATATTCTTGa  <  3:143583/136‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                     
CTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTGCTGATTAAAATGCAGCGCGAGCGCATTCATATGGCTATATTGTCGGATGAATACGGAGGAACCTCGGGACTTGTGACGACAGAGGATATTCTTGA  >  CP006881/999337‑999600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑