BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,750	C→A	5.1%	intergenic (+35/‑121)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,750	0	C	A	5.1%	13390.7 / 65.7	4239	intergenic (+35/‑121)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base C (221/3554); new base A (377/21); total (658/3581)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4727 total aligned reads displayed.

ATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC  >  Exported/3612‑3888

 tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  8:343784/139‑1 (MQ=255)

 tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  2:240217/139‑1 (MQ=255)

 tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  2:399774/139‑1 (MQ=255)

 tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  6:330433/139‑1 (MQ=255)

 tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  8:228593/139‑1 (MQ=255)

 tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  6:61755/139‑1 (MQ=255)

 tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  7:296213/139‑1 (MQ=255)

 tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  7:16714/139‑1 (MQ=255)

 tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  1:146321/139‑1 (MQ=255)

 tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  5:382952/139‑1 (MQ=255)

 gacaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  1:328934/136‑1 (MQ=255)

   caGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                          <  7:373023/138‑1 (MQ=255)

   aaGCAATGTAACACGCCAAAGTAAACAATTTAAGTACCATTTCTTATGAGCAAGTATTGTCTATTATTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGTGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                          <  2:324620/139‑1 (MQ=255)

   aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac                                                                                                                                             <  3:251499/136‑1 (MQ=255)

   aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac                                                                                                                                             <  3:150958/136‑1 (MQ=255)

   aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                          <  5:411799/139‑1 (MQ=255)

   aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTAATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                          <  5:82448/139‑1 (MQ=255)

    agaaaTCAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGt                                                                                                                                         <  1:377977/136‑1 (MQ=255)

      cAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                          <  3:5614/136‑1 (MQ=255)

         tGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTa                                                                                                                                    <  8:255607/139‑1 (MQ=255)

           aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTaa                                                                                                                                   <  8:159137/138‑1 (MQ=255)

                cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                             <  7:14223/139‑1 (MQ=255)

                       gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGga                                                                                                                         <  4:363846/136‑1 (MQ=255)

                       gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGga                                                                                                                         <  3:184307/136‑1 (MQ=255)

                       gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                                      <  7:100211/139‑1 (MQ=255)

                        tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                     <  8:294388/139‑1 (MQ=255)

                         aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                     <  2:204329/138‑1 (MQ=255)

                          aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                                      <  4:330679/136‑1 (MQ=255)

                            cAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaa                                                                                                                    <  3:287375/136‑1 (MQ=255)

                            cAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAatt                                                                                                                 <  6:380464/139‑1 (MQ=255)

                               tttAAGTACCATTACTTATGATGATGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTTGAAAGTTACACGTTACTAAAGGGAATGGATATAAatt                                                                                                                 <  4:51443/136‑1 (MQ=255)

                               tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTTGTAATTTACACGTTAATAAAGTGAATGGAGATAAattatt                                                                                                              >  2:284717/1‑139 (MQ=255)

                               tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                              <  7:1954/139‑1 (MQ=255)

                               tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                              <  2:142815/139‑1 (MQ=255)

                               tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                              <  7:54203/139‑1 (MQ=255)

                               tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                              <  5:203980/139‑1 (MQ=255)

                               tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                              <  5:145373/139‑1 (MQ=255)

                               tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                              <  2:175456/139‑1 (MQ=255)

                                  aaGTACCATTTCTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                            <  6:221637/138‑1 (MQ=255)

                                  aaGTACCATTACTTATTATAAAGTATTGTATATTTTTAATATTTATATATTATTTAACGTGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTATAGTGAATGGAGATAAattat                                                                                                               <  4:248467/135‑1 (MQ=255)

                                  aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGAAAGTTTAAAGTTACTAAATGGAATTGAGATAAattttt                                                                                                              >  3:119355/1‑136 (MQ=255)

                                  aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                            <  7:7907/138‑1 (MQ=255)

                                   aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                          <  5:291278/139‑1 (MQ=255)

                                   aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                          <  7:402366/139‑1 (MQ=255)

                                    gTACCATTACTTATGGGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                         <  6:354414/139‑1 (MQ=255)

                                       ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAATGTTACACGTTACTAAAGGGTATGGAGATAAATTATTAGAtata                                                                                                       <  6:109111/138‑1 (MQ=255)

                                       ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                       <  2:52495/138‑1 (MQ=255)

                                       ccAATACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTTTATATTTAAAAGGTACTAAATGGCATTGATAAAAATAATTAAAtata                                                                                                       >  7:265168/1‑138 (MQ=255)

                                         aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATAtacta                                                                                                    <  6:296023/139‑1 (MQ=255)

                                             cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑a                                                                                               <  6:411849/139‑1 (MQ=255)

                                               tATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTTAATTTTTTAATTTTCACGTTAAGAAATGGTATGGTGATAAATTATTATATATAATAATG‑Ac                                                                                              >  1:228467/1‑138 (MQ=255)

                                                 tGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGc                                                                                           <  7:121616/139‑1 (MQ=255)

                                                   aGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTTATTTTTTAATTTTCAACTTAAAAAAAGGAATGTAGGTAAATTATTTTATATTATAATT‑ACAGc                                                                                           >  2:102318/1‑137 (MQ=255)

                                                      aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCtt                                                                                         <  4:285863/136‑1 (MQ=255)

                                                      aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCtt                                                                                         <  3:35173/136‑1 (MQ=255)

                                                      aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TATTTTAAATTTGGAAAGTTACACGTTACAAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCa                                                                                      <  7:239577/139‑1 (MQ=255)

                                                       aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTc                                                                                        <  4:201367/136‑1 (MQ=255)

                                                        gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCaag                                                                                    <  7:21692/139‑1 (MQ=255)

                                                          aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGTAGGAAATTATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAATTTTTTAGATATACTACTG‑ACAGCTTCCaaga                                                                                   <  8:381671/138‑1 (MQ=255)

                                                          aTTGTCTATTTTTAATAGTTATCTATTAATTAACGGGATGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTACAGGGAATGGAGATAAATTATTAGATATACTACTT‑ACAGCTTCCaaga                                                                                   <  8:198504/138‑1 (MQ=255)

                                                           ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCaagaag                                                                                 <  7:345295/139‑1 (MQ=255)

                                                           ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCaagaag                                                                                 <  1:305862/139‑1 (MQ=255)

                                                             gTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTTATTTTTTAATTTTAACGTTTATAAATTGAATTGTGATTAATTATTATATTTAATAATGTACA‑CTTCCAAGAAGc                                                                                >  2:358051/1‑138 (MQ=255)

                                                              tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTa                                                                              <  8:13478/139‑1 (MQ=255)

                                                                tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCt                                                                               <  4:84051/136‑1 (MQ=255)

                                                                    tttAATTGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAagag                                                                         <  1:168334/138‑1 (MQ=255)

                                                                    tttAATAGTTATCTATTATTTACCGGGAGGAAATAATTCTATGAGTCTC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAagag                                                                         <  7:319079/138‑1 (MQ=255)

                                                                      tAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTAAATTTTGTAAATTACACGTTAATAAAGGGAATGGAGATAAATTATTATATATAATAATG‑ACACCTGACAACAAGCTAAagag                                                                         >  3:41431/1‑136 (MQ=255)

                                                                           tttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTAGC‑TTTTTTAATTTTGTAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTa                                                                  <  6:136049/137‑1 (MQ=255)

                                                                           gTTATCTATAATTTAACGGGAGGAAATACTTCTATGCGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAAATACTACTG‑TCAGCTTCCAAGAAGCTAAAGAGGTCCCTAg                                                                 <  8:104541/139‑1 (MQ=255)

                                                                                   ttattTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCt                                                            <  3:319534/136‑1 (MQ=255)

                                                                                     attTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                        <  1:125958/138‑1 (MQ=255)

                                                                                     attTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAAAGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTa                                                           <  3:84301/135‑1 (MQ=255)

                                                                                         aaCGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTATTTTGTAAGTTTAAACGTT‑CTAAATGGTATGGTGATAAATTATTTTTTTTTATACTG‑ACAGCGTGCAAGAAGCTAAAGAGGTCCCGAGCGCCGTCGGGGagg                                                   >  2:92576/1‑137 (MQ=255)

                                                                                           cGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATAATACTG‑AAAGCTTCCAAGAATCGAAATAGGTCCCTATCGCCTAAGGGGAAttt                                                 >  1:25941/1‑139 (MQ=255)

                                                                                                          cTATGAGTCGC‑TTTTTTATATTTGGAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                   <  1:4634‑M1/138‑14 (MQ=255)

                                                                                                             tgcctcgc‑tTTTTTAAATTTTGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                               <  1:404305‑M1/135‑18 (MQ=255)

                                                                                                             tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                               <  1:101217‑M1/139‑18 (MQ=255)

                                                                                                             tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                               <  8:72856‑M1/139‑18 (MQ=255)

                                                                                                             tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                               <  5:345208‑M1/139‑18 (MQ=255)

                                                                                                             tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                                  <  3:222590‑M1/136‑15 (MQ=255)

                                                                                                             tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                                  <  4:119355‑M1/136‑15 (MQ=255)

                                                                                                             tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACAG‑ACAGCTTCAAAGAATCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                               <  8:420009‑M1/139‑18 (MQ=255)

                                                                                                              gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTTAATGGAATGTATATATATTATTAGATATAATAATG‑ACAGATTCAAATATGATAAAGAGGTCCCTAGCGCCTACGGTGAATTTGgggtacattgaaat                                  <  4:161216‑M1/135‑15 (MQ=255)

                                                                                                               aGTGGC‑TTTTTTAAATTTTGAAAGTTACAAGTTACTAAAGGGAATGGAGTTAAATTATTAGATATACTACTG‑ACAGCTTCCAACAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                             <  1:285006‑M1/139‑20 (MQ=255)

                                                                                                               aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaag                                <  4:405406‑M1/136‑17 (MQ=255)

                                                                                                               aGTCGA‑TTTTTTAAATTTTTAAAGTTACTCGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAATGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                             <  1:74712‑M1/139‑20 (MQ=255)

                                                                                                                ggcgc‑TTTTTTAAATTTGGAAAGTTACACGTTAATAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                             <  8:321833‑M1/136‑20 (MQ=255)

                                                                                                                gTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaag                                <  3:380854‑M1/135‑17 (MQ=255)

                                                                                                                   gC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAAATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagag                          <  6:192431‑M1/138‑23 (MQ=255)

                                                                                                                       tttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                       <  1:201804‑M1/138‑26 (MQ=255)

                                                                                                                       tttttAAATTTGGAAAGTTAAACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAATAAGATAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                       <  5:123396‑M1/138‑26 (MQ=255)

                                                                                                                        ttttAAATTTGTAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATATAATACTG‑ACAGCTTCCAAGAAGCTAATGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                       <  5:230212‑M1/137‑26 (MQ=255)

                                                                                                                        ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                       <  6:257633‑M1/137‑26 (MQ=255)

                                                                                                                            aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                  <  6:1625‑M1/138‑31 (MQ=255)

                                                                                                                                  ggAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaaca           >  1:351615‑M1/1‑102 (MQ=255)

                                                                                                                                           cacGTTGCTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt  <  5:256866‑M1/139‑47 (MQ=255)

                                                                                                                                           cacGTTACTAAAGGTAATGGAGATAAATTATTATATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt  <  5:50555‑M1/139‑47 (MQ=255)

                                                                                                                                           cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt  <  5:173560‑M1/139‑47 (MQ=255)

                                                                                                                                           cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt  <  5:321873‑M1/139‑47 (MQ=255)

ATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTG‑ACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC  >  Exported/3612‑3888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`