Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 3,783 G→T 5.7% intergenic (+68/‑88) eryR → / → ampR /vntifkey=4//vntifkey=4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported3,7830GT 5.7% 15414.2 / 132.8 4810intergenic (+68/‑88)eryR/ampR/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand):  ref base G (1435/2952);  new base T (341/55);  total (1794/3016)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4957 total aligned reads displayed.

TAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC  >  Exported/3645‑3924
                                                                                                                                           |                                                                                                                                             
 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGCGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                                               <  1:204033/138‑1 (MQ=255)
 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                                               <  7:48036/138‑1 (MQ=255)
  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                                                             <  1:125790/139‑1 (MQ=255)
  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                                                             <  2:363489/139‑1 (MQ=255)
   gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAAAGGAGATAAATTATTAGata                                                                                                                                            <  7:171202/139‑1 (MQ=255)
      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTATTTTTTAAATTTA‑AAATTAATAAATGGGATGGAGTTAAATTTTTATAtttaa                                                                                                                                         >  6:128456/1‑138 (MQ=255)
      ccATTACTTATGAGCAAGTAATGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                                                          <  6:351173/138‑1 (MQ=255)
        aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGAAATTTTCACGTTTGAAAATGGTATTGATATAAATTATTATAtttaaa                                                                                                                                        >  8:298347/1‑136 (MQ=255)
            cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTTATTTTTTAATTTTAAAGTTAAGAAAGTGAATGGATATAAATTATTTTatatttgta                                                                                                                                      >  4:195998/1‑131 (MQ=255)
               aTGAGCAAGTAATGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                                                                  <  2:182931/137‑1 (MQ=255)
                  aGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TGTTTTAAATTTTGAAAATTAAAAGTTTATAAAGGGAATGGTGATAAATTATTTTATATAATACTTACAGCt                                                                                                                              >  6:94605/1‑138 (MQ=255)
                    cAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAATTTTAACCTTTATAAATGGTATTGTTTTAAATTATTATATTTAATAATTACAGCt                                                                                                                              >  3:148699/1‑136 (MQ=255)
                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                          <  5:15805/139‑1 (MQ=255)
                     aaGTATTGGATATTTTTAATAGTTATCTATTATTTCACGTGAGGAAATTATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGcat                                                                                                                             <  3:182495/136‑3 (MQ=255)
                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                                        <  8:157809/139‑1 (MQ=255)
                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                                        <  5:185875/139‑1 (MQ=255)
                        tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                                                       <  8:217615/139‑1 (MQ=255)
                        tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                                                       <  5:27731/139‑1 (MQ=255)
                        tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                          <  3:42255/136‑1 (MQ=255)
                           tGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                                                                     <  7:120571/138‑1 (MQ=255)
                             tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGTAAGTTTCACGTTACTAAAGGGGATGGAGATAAATTATTAGATATAATACTTAAAGCTTGCAAGAAGCt                                                                                                                   >  7:146802/1‑138 (MQ=255)
                                 tttttAATAGTTATGTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag                                                                                                               <  1:311589/138‑1 (MQ=255)
                                 tttttAATAGTTATCTATTATTTAACGGGATGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                                                                <  5:367629/137‑1 (MQ=255)
                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAAACGTTAATAAAGGGAATGGAGATAAATTATTATATATAATACTGACAGCTTCCAAGAAGCTAAatag                                                                                                             >  7:74154/1‑139 (MQ=255)
                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTTAGTTACCAGTTAAGATATGGTCTGGTGATATAATATTTTATATAATACTGACAGCGTCCAAGAAGCTAAagag                                                                                                             >  5:338921/1‑139 (MQ=255)
                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGAAATTTACACGTTACTAAAAGGAATGGAGATAAATTATTATATTTAATACAGACACCATCCAACAAACTAAaaag                                                                                                             >  8:123982/1‑139 (MQ=255)
                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                                                                <  4:422979/135‑1 (MQ=255)
                                   tttAATAGTTATATATTTTTTAACGGGAGGAAATAATTCTTTGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAGTAAAGGGAATGGATTTAAATTATTAGATATACTACTGACTGCTTCCAAGAAGCTAAagag                                                                                                             <  5:128193/138‑1 (MQ=255)
                                          gTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAg                                                                                                     <  6:411353/139‑1 (MQ=255)
                                                    attTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTa                                                                                               <  4:312110/135‑1 (MQ=255)
                                                     tttAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAACGGGAATGTAGATAAATTATTAGATATCCTCCTGCCAGCTTCCAAGACGCTAAAGAGGGCCCTAGCGCCTAc                                                                                              <  3:237314/135‑1 (MQ=255)
                                                         aCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTTGAAAGTTACACGTTACTAAATGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                                                                       <  7:388593/138‑1 (MQ=255)
                                                             tatgaaatAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                                                                  <  8:186724‑M1/136‑3 (MQ=255)
                                                              aggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                                                                 <  8:67090‑M1/139‑4 (MQ=255)
                                                                  aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                             <  2:50751‑M1/139‑8 (MQ=255)
                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  1:59398‑M1/139‑18 (MQ=255)
                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                                                                      <  4:119355‑M1/136‑15 (MQ=255)
                                                                             gAGTCGC‑TTTTTTAAATTTGGAAATTTACACTTTAATAAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  1:417253‑M1/138‑18 (MQ=255)
                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  1:390154‑M1/138‑18 (MQ=255)
                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  1:339135‑M1/138‑18 (MQ=255)
                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  1:84346‑M1/138‑18 (MQ=255)
                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGATAAACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGATTTTGgggtacattgaaaaagg                                                                   <  5:428100‑M1/138‑18 (MQ=255)
                                                                              aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAACGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagga                                                                  <  7:254537‑M1/138‑19 (MQ=255)
                                                                              aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                 <  8:256807‑M1/139‑20 (MQ=255)
                                                                                      tttttAAATTTGGAAAGTTACACGTTACTAAAGGCAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagag                                                              <  4:273325‑M1/135‑23 (MQ=255)
                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTTCGGGGAATTTGgggtacattgaaaaaggaagag                                                              <  3:118223‑M1/134‑23 (MQ=255)
                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                           <  6:185123‑M1/137‑26 (MQ=255)
                                                                                            aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt                                                    <  8:324208‑M1/139‑33 (MQ=255)
                                                                                            aaTTTGGAAAGTTACAAGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGATGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt                                                    <  5:404151‑M1/139‑33 (MQ=255)
                                                                                            aaTTTGGAAAGTTACAAGATACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt                                                    <  6:320011‑M1/139‑33 (MQ=255)
                                                                                                    aaGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAATGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaaca                                               >  4:156717‑M1/1‑99 (MQ=255)
                                                                                                          cacGTTACTAACGCGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccg                                         <  4:6704‑M1/136‑44 (MQ=255)
                                                                                                          cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATTCTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                      <  1:287657‑M1/139‑47 (MQ=255)
                                                                                                          cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                      <  6:290800‑M1/139‑47 (MQ=255)
                                                                                                          cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                      <  5:248301‑M1/139‑47 (MQ=255)
                                                                                                          cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                      <  2:24398‑M1/139‑47 (MQ=255)
                                                                                                          cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccg                                         <  3:361299‑M1/136‑44 (MQ=255)
                                                                                                          aactttactaAAGGGAATGGATTAAATTTTTTAGATTAACAACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattaaacatttccgtgt                                      <  2:216887‑M1/135‑47 (MQ=255)
                                                                                                            cGTTACTAAACGGAATTGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtg                                       <  4:236818‑M1/136‑46 (MQ=255)
                                                                                                               tACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTAc                                                                                              <  5:72618/78‑1 (MQ=255)
                                                                                                                aCTTAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAGTTGgggtactttgaaaagggaagagtatgagtattcaacttttccgtgtcgccct                                >  2:284720‑M1/1‑87 (MQ=255)
                                                                                                                aCTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcg                                    <  3:304435‑M1/135‑49 (MQ=255)
                                                                                                                 cTAAAGGGAATGGAGATATATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccgtt                               >  8:417361‑M1/1‑86 (MQ=255)
                                                                                                                 cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                                >  6:380307‑M1/1‑86 (MQ=255)
                                                                                                                 cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                                >  8:348912‑M1/1‑86 (MQ=255)
                                                                                                                 cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                      >  1:100222‑M1/1‑86 (MQ=255)
                                                                                                                   aaaGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                                >  4:347269‑M1/1‑84 (MQ=255)
                                                                                                                    aaGGGAATGGAGATAAATTATTAGATCTACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttatt                            <  6:250826‑M1/139‑57 (MQ=255)
                                                                                                                    aaGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                           >  7:200661‑M1/1‑83 (MQ=255)
                                                                                                                    aaGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagag                                                              >  3:391265‑M1/1‑83 (MQ=255)
                                                                                                                     aGGGAATTGAGATAAATTATTATAAATACTACTGTCAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattc                           <  2:322753‑M1/139‑58 (MQ=255)
                                                                                                                     aGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattc                           >  6:157195‑M1/1‑82 (MQ=255)
                                                                                                                       ggAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGCGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccc                         >  2:112173‑M1/1‑80 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGTGAATTTGgggtacattgaaaaaggaagagtat                                                           <  7:280453‑M1/103‑26 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                       >  8:383874‑M1/1‑78 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                       >  7:223256‑M1/1‑78 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                       >  7:313586‑M1/1‑78 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                       >  1:171567‑M1/1‑78 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                       >  7:96940‑M1/1‑78 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                                                      >  2:399268‑M1/1‑78 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                           <  7:339497‑M1/103‑26 (MQ=255)
                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGCAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                       >  2:85325‑M1/1‑78 (MQ=255)
                                                                                                                           tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                     >  6:100783‑M1/1‑76 (MQ=255)
                                                                                                                           tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                      <  2:150214‑M1/122‑47 (MQ=255)
                                                                                                                           tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                      >  1:150214‑M1/1‑76 (MQ=255)
                                                                                                                           tGGAGATAAATTATTAGATATACAACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                     >  1:363270‑M1/1‑76 (MQ=255)
                                                                                                                            ggAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                       >  4:9670‑M1/1‑75 (MQ=255)
                                                                                                                            ggAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                       >  3:337283‑M1/1‑75 (MQ=255)
                                                                                                                            ggAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                                                       >  5:30660‑M1/1‑75 (MQ=255)
                                                                                                                            ggAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGACCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccg                                         >  3:92413‑M1/1‑75 (MQ=255)
                                                                                                                              agaTAAATTATTAGATATACTACTGACAGCATCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttg                  <  7:363259‑M1/139‑67 (MQ=255)
                                                                                                                               gaTAAATTATTATATATACAACTGACAGCTTCCAACAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttt                    <  3:271497‑M1/136‑65 (MQ=255)
                                                                                                                               gaTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgtgtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttg                  <  1:229739‑M1/138‑67 (MQ=255)
                                                                                                                               gaTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgc                 <  1:259973‑M1/139‑68 (MQ=255)
                                                                                                                                aTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcg                                    >  6:214221‑M1/1‑71 (MQ=255)
                                                                                                                                 tAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTG                                                                                    <  4:385201‑M1/70‑1 (MQ=255)
                                                                                                                                   aaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcg                >  3:66708‑M1/1‑68 (MQ=255)
                                                                                                                                   aaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaag                                                                    <  3:21348‑M1/84‑17 (MQ=255)
                                                                                                                                      tattatATATACTAATGAAAGCTTCAAAGAAGATAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcgtcatt           <  6:59824‑M1/138‑74 (MQ=255)
                                                                                                                                        ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                                                                       >  5:217340/1‑60 (MQ=255)
                                                                                                                                           |                                                                                                                                             
TAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC  >  Exported/3645‑3924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑