BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	4,642	G→T	5.3%	V258F (GTT→TTT)	ampR →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	4,642	0	G	T	5.3%	10237.3 / 72.4	3439	V258F (GTT→TTT)	ampR	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base G (2189/916); new base T (7/316); total (2201/1238)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3660 total aligned reads displayed.

GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTT  >  Exported/4504‑4779

gATAAAGTTGCAGGCCCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAGGCCCTCCCGTATCGTAg                                                                                                                                           >  2:400075/1‑139 (MQ=255)

gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAg                                                                                                                                           >  2:322326/1‑139 (MQ=255)

gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAg                                                                                                                                           <  6:71419/139‑1 (MQ=255)

 aTAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGt                                                                                                                                          >  5:271082/1‑139 (MQ=255)

  tAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAAAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGtt                                                                                                                                         <  1:276734/139‑1 (MQ=255)

   aaaGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAg                                                                                                                                           >  3:296616/1‑136 (MQ=255)

    ctgTTGCAGCCCCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGGTTATTGCGGATAAAGCTGGAGCCGGGGAGCGTGGGTCTCGCGGTATCATTGCCGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTAt                                                                                                                                       <  8:251310/137‑1 (MQ=255)

    aaggtccAGGACCACTTCTGCGCGCGGCCCTACCGGCTGGCGGGTTTATTGCTGATAAATGTGGAGCCGGGGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTAt                                                                                                                                       <  6:365692/133‑1 (MQ=255)

    aaGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTAt                                                                                                                                       <  8:75049/139‑1 (MQ=255)

         gCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAAGCTGGCGCCGGTGCGCGGGGGTCTCGCGGTAGCATTGCAGCACTGGGGCCA‑GATGTTAAGCCCTCCCGTATCGTAGTTATCtcc                                                                                                                                   <  5:170218/138‑3 (MQ=255)

          cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                 >  8:59790/1‑139 (MQ=255)

          cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                 >  7:216253/1‑139 (MQ=255)

          cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                 >  2:124203/1‑139 (MQ=255)

          cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTAGCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                 >  6:420455/1‑139 (MQ=255)

          cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTGTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTACGCCCTCCCGTATCGTAGTTATCTccac                                                                                                                                 >  6:248261/1‑139 (MQ=255)

             ccccACTTGTGCGCGCGGCCCTGCCGGCTGGCGGGTTTATTGCTGATAAATCTGGCGCCGGGGCGGGGGGGTCGCGCGGTATCATTCCAGCACGGGGGCCA‑GATGGAAAGCCCACCCGTATCGTATTTATCAacac                                                                                                                                 <  4:204563/134‑1 (MQ=255)

                  cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTCTCTACACGACggg                                                                                                                           >  8:103685/1‑137 (MQ=255)

                  cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGCCgg                                                                                                                            >  4:413868/1‑136 (MQ=255)

                  cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACggg                                                                                                                           >  7:161778/1‑137 (MQ=255)

                     cTGCGCTGGGCCCTTCGGGCTGGGGGTTTTTTTGGTTATAAATCTGTATCGGGTTCGGGTGGGTCTGGGGGTTTAATTCCAGATCTGGGGCCA‑TTTGTTAAGCACTCCCGTATGGTTGTTATCTACACGACGGGGAGt                                                                                                                       <  8:345409/138‑1 (MQ=255)

                     cTGCGCGCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGGGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACgggg                                                                                                                          <  3:168685/135‑1 (MQ=255)

                       gcgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATATATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCTCTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTAGACGACGGGGAGTCAg                                                                                                                    >  8:412740/1‑139 (MQ=255)

                         gcTCGGCCTTGCGGGGTGGTGGGTTTTTTGCTTATAAATCGGTCGCGGGTGTGCGGGGGGCGCGCGTTATCTTTGCAGCCCTGGGGCCT‑GTTGTTAAGCCCACCCGTCTCTTATTTATATACACGACGGGGAGTCAGGc                                                                                                                  <  8:321225/139‑1 (MQ=255)

                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCTTAGTTATCTACACGACGGGTAGTCAGGCAACt                                                                                                              >  5:245691/1‑139 (MQ=255)

                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                               >  1:43772/1‑138 (MQ=255)

                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                               >  8:159531/1‑138 (MQ=255)

                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                               >  7:393025/1‑138 (MQ=255)

                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                               >  6:164513/1‑138 (MQ=255)

                             ggCCCTTCCGGCTGGCTGGTTTATTGATGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                               >  6:144394/1‑138 (MQ=255)

                                     cGGCTGGCTGTTTTATTGATGATAATTCTGGAGCCGGTGAGGGGGGGGCTCGCGGTATCATTCCACCACTGGGGCCA‑GATGGTAAGCCCCCCCTTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                      <  6:215704/139‑1 (MQ=255)

                                      ggcggggtGGTTTTTTGCTGATATCTCTGGCGGCGGTGTGGGGGGGGCGCGGGGTTTCAGTCCCGCCGTGGGCCCA‑GAGGCTACCCCCGCCCGTTTGGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGaa                                                                                                     <  2:48830/139‑1 (MQ=255)

                                         tggctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCTTAGTTATCTACACGACGGGGAGGCAGGCAACTATGGATGAACg                                                                                                   >  5:311065/1‑138 (MQ=255)

                                          ggcgggTTTTTTGCTGATAACGCGGGCGCGGGTGCGCGGGGGTCTCGCGTTATCATTGCAGCCCGGGGGCCT‑GATGGAACCCCCTCCCGTATCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGaa                                                                                                 <  2:223113/139‑1 (MQ=255)

                                                     tGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGa                                                                                            >  5:392140/1‑133 (MQ=255)

                                                         gATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACCCGACGGGGAGTCAGGCAACTATGGATGCACGAAATAGACAGATCGCTg                                                                                  >  6:281892/1‑139 (MQ=255)

                                                         gATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                   >  1:167044/1‑138 (MQ=255)

                                                          aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGtt                                                                                                                                         >  5:424157/1‑83 (MQ=255)

                                                          aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                   >  1:129342/1‑137 (MQ=255)

                                                             attgtGGCGCCGGTGCGGGGGGGGCTCGCGGTATCTTTGCCGCCCGGGGGCCA‑TATGGTACGCCCTCCGGTATGGTCTTTATATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAt                                                                              <  6:391724/135‑1 (MQ=255)

                                                             aaTCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                   >  3:375380/1‑134 (MQ=255)

                                                               tCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAg                                                                            >  1:192821/1‑139 (MQ=255)

                                                                      cgggTGCGCGGGGGGCGCGCGGTATCATGGCCGCACTGGGGCCA‑GATGGTACCCCCCCCCGTATCGTCGTTATCTACACGACGGGGAGGCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGGGc                                                                        <  3:212853/134‑1 (MQ=255)

                                                                      ccGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCt                                                                      >  5:193330/1‑138 (MQ=255)

                                                                      ccGGGGAGGGGGGGGCTCGCGGTATCATTGCCCCACTGGGGCCC‑GATGGTACGCCCGCCCGTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTc                                                                     <  1:64804/139‑1 (MQ=255)

                                                                       cggggcgCGTGGGGCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCGCCCGTATCGTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGcc                                                                       <  4:116812/130‑1 (MQ=255)

                                                                            aGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTGAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                                  >  4:241682/1‑136 (MQ=255)

                                                                             gggggggTCTCGGGTTCTCATTGCTGC‑CTGGGGCCATGTTGGAACGCCCGCCGGTTTTTTTTTTGTCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                              <  7:81806/135‑1 (MQ=255)

                                                                                  ggTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCa                                                         >  5:343423/1‑139 (MQ=255)

                                                                                   ggccggcgGTATCATTGCAGCCCGGGGGCCA‑GATGGTAAGCCCGCCCGTAGCGTAGTTGTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAt                                                        <  8:225782/134‑1 (MQ=255)

                                                                                      tcGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTgg                                                     >  1:316397/1‑139 (MQ=255)

                                                                                      tcGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATCGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTgg                                                     >  1:353101/1‑139 (MQ=255)

                                                                                                 ttGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                              >  3:190273/1‑135 (MQ=255)

                                                                                                     cgctcgggggCCA‑GTTGGTAAGCCCCCCCTTATCTTAGTTAACAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCaa                                      <  8:268652/133‑1 (MQ=255)

                                                                                                      gcaCTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAt                                                        <  6:189143/120‑1 (MQ=255)

                                                                                                        aCTGGGGCCA‑GAGGGTAAGCCCTCCGGTCTGGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCaa                                      <  4:344626/136‑1 (MQ=255)

                                                                                                         cTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCACGtt                                   >  2:72769/1‑138 (MQ=255)

                                                                                                         cTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTCTCAGACCAAg                                     >  4:382135/1‑136 (MQ=255)

                                                                                                          tGGGGCCT‑CTTGGTATGCCCTCCCGTATGTTTGTAATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                 <  8:135715/139‑1 (MQ=255)

                                                                                                          tGGGGCCC‑TTTGGAAAGCCCTCCCGTAGCGTAGTTACCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                 <  5:221980/139‑1 (MQ=255)

                                                                                                          tGGGGCCA‑GATGGTACGCCCGCCGGTATCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                 <  5:70894/139‑1 (MQ=255)

                                                                                                          tGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGa                                                                                        >  7:264919/1‑84 (MQ=255)

                                                                                                          gggggCCA‑GTTGGAACCCCCTCCCGTTTCGTCTTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGt                                    <  4:295826/135‑1 (MQ=255)

                                                                                                            gggCCT‑GTTGATAAGCCCGCCGGTCTGGTAGTTCTCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                 <  5:132348/137‑1 (MQ=255)

                                                                                                            gggCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                               >  7:61464/1‑139 (MQ=255)

                                                                                                            gggCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCACACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                               >  2:171511/1‑139 (MQ=255)

                                                                                                             ggCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                 >  3:333187/1‑136 (MQ=255)

                                                                                                               ccA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGATGAGATAGGTGCCTCACTGATTAAGCTTTGGTAACTGTCAGACCAAGTTTACt                               >  3:13585/1‑136 (MQ=255)

                                                                                                                 a‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                 >  8:293699/1‑132 (MQ=255)

                                                                                                                  agttGGTACGCCCGCCCTTATCTTAGTTAGCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                          <  5:104885/136‑1 (MQ=255)

                                                                                                                    ttgtcaaGCCCTCCCTTTTGGTTGATAACACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGGGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCa                             <  4:182438/129‑1 (MQ=255)

                                                                                                                    aTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                 >  7:102703/1‑130 (MQ=255)

                                                                                                                        tAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATAcct                    >  7:136324/1‑137 (MQ=255)

                                                                                                                            cccTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCTTATATACtt                    >  3:248560/1‑135 (MQ=255)

                                                                                                                             ccTCCCGTCTCTTAGAAAACTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTa                  <  4:69650/136‑1 (MQ=255)

                                                                                                                              cTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                          <  7:422645/127‑1 (MQ=255)

                                                                                                                                cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                          >  2:248817/1‑125 (MQ=255)

                                                                                                                                cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga            >  5:154407/1‑139 (MQ=255)

                                                                                                                                cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga            >  2:278519/1‑139 (MQ=255)

                                                                                                                                cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga            >  7:282531/1‑139 (MQ=255)

                                                                                                                                cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga            >  1:88848/1‑139 (MQ=255)

                                                                                                                                 ccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattgat           >  1:253970/1‑139 (MQ=255)

                                                                                                                                 ccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattgat           >  7:368064/1‑139 (MQ=255)

                                                                                                                                 ccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgatt              >  3:46720/1‑136 (MQ=255)

                                                                                                                                   gTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga            >  3:102146/1‑136 (MQ=255)

                                                                                                                                   gTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga            >  3:403839/1‑136 (MQ=255)

                                                                                                                                   gTATCGTAGGTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCGTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTCCTCATATATACTTTAgattga            >  4:151118/1‑136 (MQ=255)

                                                                                                                                    tATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAtt                                                       >  3:82626/1‑92 (MQ=255)

                                                                                                                                      tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAt                                                                              >  8:198101/1‑67 (MQ=255)

                                                                                                                                      tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTaa       >  7:33281/1‑138 (MQ=255)

                                                                                                                                         tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                          <  1:39561/116‑1 (MQ=255)

                                                                                                                                         tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt   >  8:380599/1‑139 (MQ=255)

                                                                                                                                         tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt   >  7:237891/1‑139 (MQ=255)

                                                                                                                                         tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt   >  6:325623/1‑139 (MQ=255)

                                                                                                                                         tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt   >  7:191868/1‑139 (MQ=255)

                                                                                                                                           gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                    >  5:373457/1‑72 (MQ=255)

                                                                                                                                           gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCa                                                         >  6:105668/1‑83 (MQ=255)

                                                                                                                                           gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                   >  7:307365/1‑105 (MQ=255)

                                                                                                                                           gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                          <  1:101114/114‑1 (MQ=255)

                                                                                                                                           gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                          <  1:225964/114‑1 (MQ=255)

                                                                                                                                           gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGATTACTCATATATACTTTAGATTGATTTAAAACta  >  2:358563/1‑137 (MQ=255)

GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTT  >  Exported/4504‑4779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`