| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 1,011,322 | T→G | 28.6% | V406G (GTT→GGT) | U712_04945 → | putative multidrug resistance ABC transporter ATP‑binding/permease protein YheH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 1,011,322 | 0 | T | G | 28.6% | 65.9 / 10.8 | 57 | V406G (GTT→GGT) | U712_04945 | putative multidrug resistance ABC transporter ATP‑binding/permease protein YheH |
| Reads supporting (aligned to +/- strand): ref base T (12/28); new base G (15/2); total (27/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.60e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGAATGCGGCGGGGATTGTTTCTATCGGTGTGCTGTACGCGTTTGTCGATTATTTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAAGAGGTATTAAAGCATAT > CP006881/1011186‑1011451 | cTGAATGCGGCGGGGATTGTTTCTATCGGTGTGCTGTACGCGTTTGTCGATTATTTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑Gttt < 1:298513/139‑1 (MQ=255) aaTGCGGCGGGGATTGTTTCTATCGGTGTGCTGTACGCGTTTGTCGATTATTTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GGTTTTg > 1:124870/1‑139 (MQ=255) gcggGGATTGTTTCTATCGGTGTGCTGTACGCGTTTGGCGATTATTTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCTCCGGGCGT‑GGTTTTGaaatag > 2:232447/1‑135 (MQ=255) cggGGATTGTTTCTATCGGTGTGCTGTACGCGTTTGTCGATTATTTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCACCGGGCGTGGTTTTTGacagcg > 6:338479/1‑134 (MQ=255) gTTTCTATCGGTGTGCTGTACGCGTTTGTCGATTATTTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGAACTGCTTgaa < 3:235410/135‑1 (MQ=255) tCGGTGTGCTGTACGCGTTTGTCGATTAATTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGAATa < 8:361114/138‑1 (MQ=255) ggTGTGCTGTACGCGTTTGTCGATTATTTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GGGTTTTAAATGGTTGatgaaaacaaa > 4:235410/1‑134 (MQ=255) tgtgCTGTACGCGTTTGTCGATTATTTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGAACTGCTTGAATAAAACAATAAGgaa > 7:216540/1‑139 (MQ=255) tgtgCTGTACGCGTTTGTCGATTATTTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GGTTTTGAACTGCTTGAAGAAAAGAATACgg > 5:144273/1‑137 (MQ=255) tgCTGTACGCGTTTGTCGATTATTTTAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GGTTTTGGAAGGAGTGAAGAAAACAAtgacg > 3:276645/1‑131 (MQ=255) tgCTGTACGCGTTTGTCGATTATTTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGTACTGGGTGAAGAAAAGAATACGgaag > 8:10485/1‑138 (MQ=255) gCTGTACGCGTTTGTCGATTATTTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GGTTTTTAACTGCTTGAAGAAAAGAATACgg > 4:390165/1‑134 (MQ=255) cgTTTGTCGATTATTTGAACGGGTTATTTCAGCCGATGACAGGCATCGTCAATCAGTTTTCAATGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGATTACGGTAGAAGCggg < 4:201581/136‑1 (MQ=255) gTTTGTCGATTATTTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GGTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGaa > 7:281551/1‑139 (MQ=255) tttGTCGATTATTTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAAc < 5:92251/139‑1 (MQ=255) tttGTCGATTATTTGAACCGGTAATTTCAGCCGAGCACCGGCATCATCAATCACTTATCAAAGCTGGAGCGTGCTAGGGTCTCCGCGGGGGGT‑TTTTTTGAACTGCTTTAACAAAAGAATACGGAAGATGCGGGTGAAc < 2:354058/139‑1 (MQ=255) tGTCGATTATTTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCAGGGCGT‑GTTTTTGTACTTCATGAAGAAAAGTATACTGAAGAAGCGGGTGCACCg > 7:231773/1‑139 (MQ=255) tattTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GGGTTTGTAATTGGGGTAGGAAAGAAAAAGGAAGAAACGGGTGGGCCGGCGAAgg > 2:352606/1‑139 (MQ=255) ttATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GGGTTTTTTATGGTTGAATGAAATAATAACGAAGAAGCGGGGGAACCGGCGATCGAGAGCGCGCggg > 2:424904/1‑139 (MQ=255) aTTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTggg < 1:352606/138‑1 (MQ=255) tttCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTGGTTGATCTGCGTGTATAAAAAAAAACAGCAGAATCGGGGGCACCTGCCAAGGAgagcgcggggg > 4:239297/1‑132 (MQ=255) gCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTTGAATGCGTTTAGAAAATAAAAAAGAAGAAGAGGGTTCTCAGGCGCAGGCGCGCGCGCGgggggggggt > 7:418975/1‑137 (MQ=255) ccGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGaa < 2:151342/72‑1 (MQ=255) ccGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGaa > 1:151342/1‑72 (MQ=255) ccGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGTACTGCTTGTATAAAATAATAAGGAAGAAGCGGGTGTAACCGCCAAGTAGCGCCCGCAGGGGGGGGtgg > 6:391471/1‑137 (MQ=255) ccGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGTACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGGACCGGCGCAGGAGCGCGCGCGGGGGGGGGGTg > 8:317657/1‑139 (MQ=255) tCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GGGTTTGTTCTGGTGGGAGATAAGAATACGGAAGGAGCGGGTGGGCCGGCGCAGGGGCGCGCGCGGGGGGGGGGTggtgt > 6:285571/1‑135 (MQ=255) aGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGt < 8:281551/139‑1 (MQ=255) tCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GGTTTTTTACTGCGTGAAGGAAAGAATACGGAAGAAGCGGGGGGACCGGCGCAGGAGCGCGCGCTGGGGGGGGGGGGATTTGGTGAggt > 2:181153/1‑139 (MQ=255) cGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGTGGTTTTTGAA‑TGGGTGAATAAAACAATACGGCAGAAGCGGGGGCACCGGCGAAGGAGCGCGCGCGGGGGGGGGGTGGATTTCGGGGtgtg > 7:302974/1‑139 (MQ=255) aaTCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GGTTTTGTAGTGCTTGGAGCAAAAAAAAAGGTAGAACCGGGGGCACCGGCGAAGGCGCGCGCGCGGGGGCGGGGTGAATTTAGTGAtttgggat > 1:147268/1‑135 (MQ=255) aTCAGTTTACAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGCCGT‑GGTTTTTTAATGCAAGAATAAAAAAATAAGCAACAACAGGGGGAGCCCGCGAACGAACCCGCGCTGGGGGGGGGGGAATAATGTTATgtgt > 4:304511/1‑135 (MQ=255) tCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGTACAGGTTGAAGATAATAATACGGAAGATGCGGGTGGCACGGCGCAGGAGGGCGCGCTGGGGCGGGGTGCTTTTAGTGGTGTGT‑CGttt > 7:195088/1‑139 (MQ=255) aGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCa < 3:418925/136‑1 (MQ=255) gCTGGAGCTTGCAAGGGTCTCCTCCGGTCGT‑TTTTTTCAACTTCTAGAAGAAAAGAATAAGGAAGAAGCGGGTGAACGGGCGAAGGAGCGCGCGGTGGGGCGGGTTGAATTTCTTGATGTGT‑CATTTGCGTATCaagaa < 1:232447/139‑1 (MQ=255) cTGGCGCTTGCAAGGGGCTCCGCCGGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCaagaag < 8:216540/139‑1 (MQ=255) cTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCaagaag < 7:86325/139‑1 (MQ=255) gAGCTTGCAAGGGTCTCCGCCGGGCGT‑GGTTTTGGAAAGCGTGATGAAAATAAATCGGAAGAAGCGGGTGAACCCGCGAAAGAGCGCCCGCTGGGGCGGGTGTTTTTTAGTGATTGGTATATTTG‑GTATCAATAAGGTg > 6:290752/1‑139 (MQ=255) aGCTTGCAAGGGTCTCCGCCGGGCGG‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAAGTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGa < 8:231773/139‑1 (MQ=255) gttGCAAGGGGCGCCGCCGGGCGT‑GTTTTTGAACTGCTTGAAGTAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTg < 4:126159/135‑1 (MQ=255) cTTGCAAGGGTCTGCGCCGGGGTT‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAag < 2:23114/139‑1 (MQ=255) cTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTg < 4:109926/136‑1 (MQ=255) ggaaGGGTCTCCAGCGGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAaga < 5:338479/135‑1 (MQ=255) gCAAGGGTCTCCGGCGGGCGT‑GTTTTTGAACTGCTTGAAGAAAATAATACGGAAGAAGCGTGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAag < 8:418975/136‑1 (MQ=255) aaGGGTCTCCGCCGGGCGT‑GTTTTTTAACAGCTTGAAGAAAATAATAAGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGGGGGTTGAATTTTGTGATTTGT‑CATTTTCGTATAAAGAAGGTGAag > 4:418925/1‑134 (MQ=255) gggTCTCCGCCGGGCGT‑GGGTTTGTAAGGGGTGAAGAAAAGAATACGGAAGAAGCGGGTGGACCGGCGAAGGGGCGCGCGCTGGGGGGGGGTGGTTTTGGTGCTGTGT‑GATTTGGGGTTCAAGAAGGGGATGTGGt > 4:292227/1‑136 (MQ=255) ggcgctcCGCCGCGCGT‑GGATGTGAGCTGCTTCAAGACAAGCATACGGCCGAAGCGGGAGCTCCGGCGCAGGAGCGCGCGCTGGGGCGGGGTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAAGAGGt < 4:283242/132‑1 (MQ=255) ggTCTCCGCCGGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAAGAGGTATTa < 1:424904/139‑1 (MQ=255) ggctcCGCCGGGCGT‑GTTTTTGCACTGCTTGAAGAAAAGAATACGGCAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCGGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAAGAGGTAt < 3:239297/134‑1 (MQ=255) cgccgGGGGT‑GTTTTTGAACTGCTTGAAGAACAGAATACGGAAGAAGCGGTTGAACAGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAAGAGGTATTAAAGCat < 5:391471/139‑1 (MQ=255) cgccgGGCGT‑GTTTTTGAACTGCTTGAAGAAACGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAAGAGGTATTAAAGCat < 5:371434/139‑1 (MQ=255) cgccgGGCGT‑GTTTTTGAACTGCTTGAAGAAAATAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCGGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAAGAGGTATTAAAg < 4:391360/136‑1 (MQ=255) cgccgGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAAGAGGTATTAAAGCat < 7:317657/139‑1 (MQ=255) cgccgGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAAGAGGTATTAAAGCat < 2:148505/139‑1 (MQ=255) cgccgGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGGGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAAGAGGTATTAAAGCat < 8:27621/139‑1 (MQ=255) cgccgGGCGT‑GGTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAAGAGGTATTAAAGCat < 6:30773/139‑1 (MQ=255) gcgGGCGT‑GTTTTTGAACTGCTTGAAGAAAATAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAAGAGGTATTAAAGCatat < 1:308762/138‑1 (MQ=255) ccgGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAAGAGGTATTAAAGCa < 3:390165/136‑1 (MQ=255) | CTGAATGCGGCGGGGATTGTTTCTATCGGTGTGCTGTACGCGTTTGTCGATTATTTGAACCGGTTATTTCAGCCGATCACAGGCATCGTCAATCAGTTTTCAAAGCTGGAGCTTGCAAGGGTCTCCGCCGGGCGT‑GTTTTTGAACTGCTTGAAGAAAAGAATACGGAAGAAGCGGGTGAACCGGCGAAGGAGCGCGCGCTGGGGCGGGTTGAATTTCGTGATGTGT‑CATTTGCGTATCAAGAAGGTGAAGAGGTATTAAAGCATAT > CP006881/1011186‑1011451 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |