BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,006	T→G	6.9%	V40G (GTT→GGT)	lacZalpha →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,006	0	T	G	6.9%	14454.7 / 195.7	6020	V40G (GTT→GGT)	lacZalpha	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base T (2613/2828); new base G (504/51); total (3135/2885)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 6069 total aligned reads displayed.

ATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTAC  >  Exported/5868‑6144

    cacacaGGACACAGCTATGACCATGATTACGCCAAGCGTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGGCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAcc                                                                                                                                        <  6:118860/139‑1 (MQ=255)

    cacacaGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAc                                                                                                                                         <  1:128728/138‑1 (MQ=255)

     acacaGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAcc                                                                                                                                        <  1:20474/138‑1 (MQ=255)

         aGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCGGGCGTTACCCAACt                                                                                                                                   >  5:85781/1‑139 (MQ=255)

            aaaCAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTaa                                                                                                                                <  5:408020/139‑1 (MQ=255)

             aaCAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTTGAGGATGCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTaa                                                                                                                                <  8:147018/138‑1 (MQ=255)

             aaCAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACt                                                                                                                                   <  4:248398/135‑1 (MQ=255)

             aaCAGCTATGACCATGATTACGACAAGCTTGCATGCCTGCAGGTCGTCTCTAGAGGATCCCCGTGTAACGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCTTGTCTGGGAAAACCCTTGCGTTACCCAACTTaa                                                                                                                                <  7:310620/138‑1 (MQ=255)

              aCAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGGCTGGGAAAACCCAGGCGTGTCCCCACtt                                                                                                                                  >  3:229711/1‑135 (MQ=255)

               cAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTaa                                                                                                                                <  4:307420/136‑1 (MQ=255)

                       aCCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTgcag                                                                                                                     >  7:201734/1‑139 (MQ=255)

                        ccATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGGAAACCCAGGCGTTACCCAACATAATAGCCGTgc                                                                                                                       >  3:85197/1‑136 (MQ=255)

                        ccATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCGTGCGTTTCCCCACTTAGTAGCCTGgc                                                                                                                       >  4:78391/1‑136 (MQ=255)

                          aTGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGcac                                                                                                                  <  7:51720/139‑1 (MQ=255)

                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGCAACCCCTGGCGTTACCCAACTTTATAGCCGTgcagcccca                                                                                                                >  5:233643/1‑135 (MQ=255)

                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAcc                                                                                                                                        <  1:240000/115‑1 (MQ=255)

                            gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACAt                                                                                                                >  7:282650/1‑139 (MQ=255)

                               tACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACAt                                                                                                                >  3:356850/1‑136 (MQ=255)

                               tACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTGTTAAACCCTCCTGACGGGGAAAACCCAGGCGGTTTCCCACACAATCGCCCTgcagccaat                                                                                                                >  4:49456/1‑132 (MQ=255)

                                    cAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGGGACGGGGAAAACCCAGGCGGTACCCCACTTAAACGCCGTGCAGCCCCTccccc                                                                                                           >  3:171470/1‑136 (MQ=255)

                                      aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTTCCCAACTTAATCGCCGTGCAGCACATCCCCCATTCg                                                                                                      >  6:219077/1‑139 (MQ=255)

                                       gCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGGCTGGGAAAACCCTGGCGTTAACCAACTTAATAGCCATGCAGCCCCTACCCCTTTAGc                                                                                                     >  2:98842/1‑139 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCCACTTAATCGCCTTGCAGCCCCTCCCCCTTTCGCCCGCt                                                                                                >  8:207968/1‑139 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACCTCCCCCTTTCGCCAGCt                                                                                                >  5:164693/1‑139 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                 <  6:370500/138‑1 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                 <  2:242444/138‑1 (MQ=255)

                                            cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                 <  7:375502/138‑1 (MQ=255)

                                               gCCTGCAGGTCGACTCTAGAGGATCACCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGCCTGGGCAAACCCAGGCGTTACCCAACTAAAACGCCATGCAGCACAACCCCCTTTCCCCCGcc                                                                                                >  3:326690/1‑135 (MQ=255)

                                                ccTGCAGGTCGACTCTATAGGATCCGCGGGTACCGAGCTCGAATTCACTTGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAAGTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCg                                                                                            <  8:312495/139‑1 (MQ=255)

                                                 cTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCg                                                                                            >  8:379177/1‑138 (MQ=255)

                                                  tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACACCGTCGTGACGGGGGAAACCCTGGCGGTTCCCAACGTAATCGCCTTGCCGGCCATCCCCCGTTCGCCCGCGGGCggt                                                                                          >  1:300198/1‑137 (MQ=255)

                                                  tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGt                                                                                           >  6:168241/1‑138 (MQ=255)

                                                    cAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCATCTGGc                                                                                             <  3:265712/134‑1 (MQ=255)

                                                    cAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTaa                                                                                         <  8:285743/138‑1 (MQ=255)

                                                     aGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCCCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAACCCCTGGCGTTACCCAACTTAATCGCCTTGCCGCCCCTCCCCCGTTCGCCAGCGGGCGt                                                                                           >  4:403389/1‑135 (MQ=255)

                                                       gTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCAGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACCTCCCCCTTTCGCCAGCTGGCGTAATAGc                                                                                     >  8:403674/1‑139 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGATGTCGAATTCACTGGCCGGCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCAAACTTAATCGCCTGGCAGCAGATCCCCCTTTCGCCAGCTGGAGTAATAGCGaa                                                                                  <  8:123780/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGc                                                                                     <  3:413109/136‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGc                                                                                     <  4:205524/136‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                  <  5:357722/139‑1 (MQ=255)

                                                          gACTCTAGAGGATCCCCGGGGACCGAGCTCTAATTCACTGGCCGTCGTTTTACACCGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                  <  5:136060/139‑1 (MQ=255)

                                                           aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGCGTTACCCAACTTAATAGCCTTGCAGCACATCCCCCGTTAGCCAGCTGGGCTAAAAGCg                                                                                    >  8:7378/1‑136 (MQ=255)

                                                               tAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGc                                                                             >  7:343333/1‑139 (MQ=255)

                                                                 gagGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGGAAACCCTGGCGTTACCCCACTTAATCGCCTTGCCGCACATGCCCCTTTCGCCAGCTGGCGTAATAGCCACGAGGccc                                                                           >  8:51358/1‑139 (MQ=255)

                                                                  agGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTAGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCg                                                                          <  1:54760/139‑1 (MQ=255)

                                                                   ggATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGc                                                                         <  7:75741/139‑1 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCCACGTAATCGCCTTGCAGCCCATCCCCCTTTAGCCAGCTGGGGTAATAGCGAAGAGGcccccccc                                                                      >  6:420616/1‑134 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAcc                                                                      >  6:144182/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCATTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAcc                                                                      >  2:47259/1‑139 (MQ=255)

                                                                      tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACATAATCGCCTTGCAGCCCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGcccccccc                                                                      >  8:2603/1‑134 (MQ=255)

                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGGCTGGGAAAACCCTGGCGGTACCCAACTTAATCGCCGTGCAGCACATCCCCCTTTCGCCAGCTGGCGGAATAGCCAAGAGGCCCGCCCCg                                                                     >  6:95260/1‑139 (MQ=255)

                                                                       ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGGCTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCCGCTGGCGGAATAGCGTCGAGGCCCGCCCCg                                                                     >  6:395783/1‑139 (MQ=255)

                                                                         ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACCACGTCGTGACGGGGCAAACCCGGGCGTTTCCCAACATAAAAGCCTTGCAGCCCAAGCCCCTTTAGCCCGCGGGCGGAAATGAGCAGAGGCCCGCCCCCAt                                                                   >  2:150893/1‑139 (MQ=255)

                                                                         ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCAGGCGTAATAGCGAAGAGGCCCGCCCCgca                                                                   >  8:23446/1‑137 (MQ=255)

                                                                         ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTAGCCAACTTAATCGCCTTGCATCACATCCCCCTTTCGCCAGCTGGGGTAATAGCGAAGAGGCCCGCACCGAt                                                                   <  7:99323/139‑1 (MQ=255)

                                                                           gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGa                                                                    <  3:53596/136‑1 (MQ=255)

                                                                           gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                 <  1:100070/139‑1 (MQ=255)

                                                                           gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                 <  7:14279/139‑1 (MQ=255)

                                                                           gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGGCGGGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                 <  7:172639/139‑1 (MQ=255)

                                                                            ggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGAt                                                                   >  3:200135/1‑136 (MQ=255)

                                                                            ggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGAt                                                                   >  4:176820/1‑136 (MQ=255)

                                                                             ggaCCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                               <  1:7786/137‑1 (MQ=255)

                                                                             gTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                               <  7:404290/139‑1 (MQ=255)

                                                                              tACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGccc                                                              <  6:331941/139‑1 (MQ=255)

                                                                                ccGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACCTCCCCCGTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCCCCGCTCGCCCtt                                                            >  6:299629/1‑139 (MQ=255)

                                                                                ccGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCtt                                                            <  5:208985/139‑1 (MQ=255)

                                                                                cagatcTCGAATTAACTGGCCGTCTTTTTACAAGGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCGTTGCAGCACATCCCCGTTTCGCCAGCTGGCGTATTAGCGAAGAGGCCCGCACCGATCGGCCtt                                                            <  8:343882/134‑1 (MQ=255)

                                                                                  gAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGGAACTGGGAAAAACCTGGCGTTACCCCACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGCAGAGGCCCGCACCGCTCGCCCGTcc                                                          >  1:267497/1‑139 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCCGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                         <  2:106783/139‑1 (MQ=255)

                                                                                   aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACATAATCGCCATGCAGCACATCCCCCGTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATAGcccgaccc                                                         >  5:381582/1‑134 (MQ=255)

                                                                                      tCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGTACACCCTGGCGTTACCCACCGAAATCGCCTTGCCGCACCTCCCCCTTTCGCCCGCTGGCGTGATAGCGGAGAGGCCCGCCCCGATAGCCCTTgccca                                                       >  6:191011/1‑136 (MQ=255)

                                                                                      tCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTACCCCACTTAATCGCCTTGCAGCCCCTCCCCCATTCGCCCGCTGGCGTAATAGCGAAGAGGCCCCCCCCCAGCGCCCCTccc                                                         >  3:134708/1‑136 (MQ=255)

                                                                                                  gCCGTCGTTGTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGc                                           <  8:183910/138‑1 (MQ=255)

                                                                                                              cAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggc                                  <  3:301959/135‑1 (MQ=255)

                                                                                                               aaggtcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaatg                             <  7:227505/136‑1 (MQ=255)

                                                                                                                 cgtcgtGACTGGGAAACCCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaat                              <  4:282001/136‑1 (MQ=255)

                                                                                                                   tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                              <  7:118597/118‑1 (MQ=255)

                                                                                                                    cgtGACTGGGAAAACCCTGGCGTTACCCAACTTAAACGCCTTGCAGCACATCCCCCGTTCGCCAGCGGGCGTAATAGCGAAGAGGCCCGCACCGAGCGCCCGTCCCACCAGTTGCGCAGCCTgaatggcgaatggc                           >  4:341539/1‑136 (MQ=255)

                                                                                                                    cgtGACTGGGAAAACCCGGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaatggc                           <  4:107878/136‑1 (MQ=255)

                                                                                                                     gtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCt                       <  5:49767/139‑1 (MQ=255)

                                                                                                                      tGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGcc                                                                                                                           >  4:143833/1‑38 (MQ=255)

                                                                                                                           gggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGAt                    <  3:384846/136‑1 (MQ=255)

                                                                                                                           gggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                 <  1:403541/139‑1 (MQ=255)

                                                                                                                            ggAAACCCCTGGCGGTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                  <  5:279981/137‑1 (MQ=255)

                                                                                                                            ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGCAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                  <  1:331071/137‑1 (MQ=255)

                                                                                                                            ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCg                                                                          >  5:188536/1‑81 (MQ=255)

                                                                                                                            ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                  <  6:662/137‑1 (MQ=255)

                                                                                                                            ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                  <  2:312049/137‑1 (MQ=255)

                                                                                                                            ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                  <  2:73108/137‑1 (MQ=255)

                                                                                                                            ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                 <  5:107283/138‑1 (MQ=255)

                                                                                                                                aaCCCTGGCGTTACCCAACTTAATCGCCGTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtt            <  6:251798/139‑1 (MQ=255)

                                                                                                                                    cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAttt           >  4:371297/1‑136 (MQ=255)

                                                                                                                                    cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct        <  1:168327/139‑1 (MQ=255)

                                                                                                                                    cTGGCGTTACCCAACTTAAGCGCCTTGCCGCACAACCCCATTTCGCCAGCGGGCGTAATAGCGCAGAGGCCCGCACCGATCGCCTTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct        <  2:204522/139‑1 (MQ=255)

                                                                                                                                      ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                  >  7:256411/1‑63 (MQ=255)

                                                                                                                                      ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                  <  2:344762/63‑1 (MQ=255)

                                                                                                                                      ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCGTCCCAACCGTTGCGCAGCCTGAATGGCGAATGGCGCCTGCTGCGGTATTTTCTcc      >  1:356842/1‑139 (MQ=255)

                                                                                                                                         gTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa   <  5:325702/139‑1 (MQ=255)

                                                                                                                                         gTTACCCAACTTAAGCGCCTTGCAGCACAACCCCCTTTCGCCAGCTGGCGTAATAGCGACGAGGCCCGCACCGATCGCCCATCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa   <  2:37673/139‑1 (MQ=255)

                                                                                                                                         gTTACCCAACTAAATCGCCTTGCAGCACATCCCACTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa   <  2:277835/139‑1 (MQ=255)

ATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTAC  >  Exported/5868‑6144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`