Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 6,012 A→C 7.0% Q42P (CAA→CCA)  lacZalpha → /vntifkey=4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported6,0120AC 7.0% 16582.1 / 239.3 6255Q42P (CAA→CCA) lacZalpha/vntifkey=4
Reads supporting (aligned to +/- strand):  ref base A (2700/2991);  new base C (541/4);  total (3256/2999)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 6303 total aligned reads displayed.

CACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCT  >  Exported/5874‑6150
                                                                                                                                            |                                                                                                                                          
     gAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGCCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCGGGCGTTACCCAACtt                                                                                                                                        >  2:65508/1‑138 (MQ=255)
      aaaCAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACt                                                                                                                                         <  4:420071/136‑1 (MQ=255)
       aaCAGCTATGTCCATGATTACGCCAAGCTTGCATGCCTGGAGGTCGACTGTAGAGGATCCCCGGGTACCGAGCTAGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTTGGAAAACCCTGGCGTTACCCAACtt                                                                                                                                        <  3:219690/136‑1 (MQ=255)
         cAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTaa                                                                                                                                      <  3:254465/136‑1 (MQ=255)
         cAGCTATGACCAAGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGAGCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTaa                                                                                                                                      <  4:124686/136‑1 (MQ=255)
           gCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGcc                                                                                                                                 <  7:170137/139‑1 (MQ=255)
               tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTT‑AC‑ACGTGCTGACGGGGGAACCCCCTGCGGTTCCCCACGTAAACTCCTTgg                                                                                                                             >  2:425902/1‑138 (MQ=255)
               tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGTAAACCCTGGCGTTACCCACCGTAATCGCCTTgc                                                                                                                             >  1:52866/1‑139 (MQ=255)
               tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAAt                                                                                                                                     <  1:13273/131‑1 (MQ=255)
               tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAAt                                                                                                                                     >  7:422499/1‑131 (MQ=255)
               tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTg                                                                                                                              <  5:299304/138‑1 (MQ=255)
                   cATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTgcagca                                                                                                                         <  1:331508/139‑1 (MQ=255)
                    aTGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGGAAACCCTGGCGGTACCCAACTTAATCGCCTTgccgccc                                                                                                                        >  1:75601/1‑137 (MQ=255)
                     tGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGcac                                                                                                                        >  5:366879/1‑138 (MQ=255)
                       aTTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGcac                                                                                                                        >  4:225624/1‑136 (MQ=255)
                       aTTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATc                                                                                                                     <  6:321872/139‑1 (MQ=255)
                         tACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGCCTGGGAAAACCCTTGCGTTACCCAACATAATCGCCTTGCAGCCCAt                                                                                                                      >  4:166477/1‑136 (MQ=255)
                         tACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGGCGGCGCAGGGAAAACCCCGGCCGTACCCAACATAATCGCCTTGCCGcaccg                                                                                                                      >  3:341190/1‑134 (MQ=255)
                             ccAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCtt                                                                                                               >  7:332828/1‑139 (MQ=255)
                                aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCt                                                                                                                                <  8:145648/119‑1 (MQ=255)
                                aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGGTACCCCACTTAATCGCCGTGCAGCACATCCCCCTTTCg                                                                                                            >  5:393812/1‑139 (MQ=255)
                                aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTGTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGGTACCCAACTTAATAGCCTTGCTGCACATCCCCCTTTCg                                                                                                            >  6:183949/1‑139 (MQ=255)
                                 gCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGc                                                                                                           >  1:354452/1‑139 (MQ=255)
                                     gCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGCGCTCGAATTCACTGGCCGTC‑GTTTT‑AAAACGTCGTGCCTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACCTAACCCATTCGCCAGc                                                                                                       >  6:416557/1‑139 (MQ=255)
                                      cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                       <  5:14962/138‑1 (MQ=255)
                                      cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                       <  6:92287/138‑1 (MQ=255)
                                      cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GGTTT‑TCAACGTCGTGGCGGGGGCAACCCGGGGGGTTCCCCACTGTATCGCCGGTCCGCCCATCCCCCGTTCGccccgg                                                                                                      >  7:366080/1‑135 (MQ=255)
                                          ccTGCCGGGCGACTCTACAGGATCCCCGGGGACCGAGCTCGAAAGCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTACTCGCCTTGCAGCACATCCCCCTTTCGCCCGCTGGCg                                                                                                  <  2:364799/139‑1 (MQ=255)
                                          ccTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCg                                                                                                  <  1:24220/139‑1 (MQ=255)
                                          ccTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCg                                                                                                  <  2:336295/139‑1 (MQ=255)
                                          ccTGCAGGTCGACTCTAGAGGATCCCCGGGGACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGGTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTg                                                                                                     <  3:96571/136‑1 (MQ=255)
                                           cTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAACTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTgg                                                                                                    <  3:160945/136‑1 (MQ=255)
                                            tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGt                                                                                                 >  8:204651/1‑138 (MQ=255)
                                              cAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTaa                                                                                               <  7:223764/138‑1 (MQ=255)
                                               aGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACCTCGTGACAGGGCAAACCCTGGCGTTACCCGAGATAAACGCCTTGCAGCACATCCCCCTTTCGCCCGCTGGCGTa                                                                                                >  3:291170/1‑136 (MQ=255)
                                                    gACTCTCGAGGACCCCGGGGTAACGAGCTCGAATTCACTGGCCGGC‑GGTTT‑CCACCGTCGGTACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTACGCCCGCTGGCGTAATAGCGaa                                                                                        <  2:270611/139‑1 (MQ=255)
                                                    gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGc                                                                                           <  3:318166/136‑1 (MQ=255)
                                                    gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCTaa                                                                                        <  6:134655/139‑1 (MQ=255)
                                                    gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                        <  7:39777/139‑1 (MQ=255)
                                                    gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                        <  8:228606/139‑1 (MQ=255)
                                                    gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                        <  7:94951/139‑1 (MQ=255)
                                                    gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                        <  8:118521/139‑1 (MQ=255)
                                                    gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGGCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                        <  2:147696/139‑1 (MQ=255)
                                                         tAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACCACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACGTAATCGCCTTGCCGCACATCCCCCTTTCGCCCGCGGGCGGAATAGCGCAGAGGc                                                                                   >  5:337707/1‑139 (MQ=255)
                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGGCGGGGTAAACCCAGGGGGTACCCAACATAATAGCCTTGGCGCCCATCCCCCATTCGCCAGCGGGGGGTAAACCGAAGATGcccccccc                                                                            >  5:25825/1‑134 (MQ=255)
                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTTCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGcccc                                                                            >  2:9061/1‑139 (MQ=255)
                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTg                                                                                                                              <  7:70685/89‑1 (MQ=255)
                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTTCAGCCCCTCCCCCATTCGCCAGCTGGCGGAATTGCGAAACGGCCCGcccc                                                                            >  5:219254/1‑139 (MQ=255)
                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACCTCCCCCTTTCGCCAGCGGGCGGAATAGCGAAGAGGCCCGCAcc                                                                            >  5:12041/1‑139 (MQ=255)
                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCAGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCGTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAcc                                                                            >  8:1783/1‑139 (MQ=255)
                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAAACCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTAGCCAGCTGGCGTAAAAGCGAAGAGGCCCGcccc                                                                            >  7:302308/1‑139 (MQ=255)
                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACAGGGAAAACCCTGGCCTTACCCCACATAAACGCCTTGCACCACAACCCCCGTTCGCCAGCGGGGGGAATAGCGCACAGGCCCGcccc                                                                            >  7:115437/1‑139 (MQ=255)
                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCCTGACATGGCAAACCCAGGCGTTACCCAACATAATCGCCTTGCAGCACATCCCCCGTTCGCCCCCGGGCGGAAAAACGAAGAGGcccccccc                                                                            >  6:359287/1‑134 (MQ=255)
                                                                 ccccGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTTAACACCGTGGTGCGGGGGAAACCCATGGCGTTCCCCACCTTAATCCCCTTGCCGCCCATCCCCCTTTCCCCCGGGGGGGTAATAGCCATGAGGcccccccc                                                                            >  2:100852/1‑134 (MQ=255)
                                                                   ccGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑TCAACGGCGTGACTGGGTAAACCCTGGCGTTACCCCACGTAATAGCCGTGGAGCACCACCCCCCTTCGCCAGCTGGCGGAATAGCGAAGAGGCCCGCACCgct                                                                         >  6:107540/1‑137 (MQ=255)
                                                                   ccGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCGGGCGGAAAAGCGAACACGCCCGcccc                                                                            >  3:124756/1‑136 (MQ=255)
                                                                   ccGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACATAATCGCCGTGCAGCACCTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAcc                                                                            >  3:428944/1‑136 (MQ=255)
                                                                   ccGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAAACCTGGCGTTACCCAACTTAATCGCCGTGCAGCACATCCCCCATTAGCCAGCAGGGCTAAAAGCGAAGAGGCCCGcccc                                                                            >  4:355330/1‑136 (MQ=255)
                                                                   ccGGGTACCGAGCTCGAATTCACTGGCCGTC‑GGTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTTACCAAATTAATCGCCATGGAGGACAACCCCCATTCGCCAGCAGGCGTAAAATCGAAGCGGCCCGCAcc                                                                            >  4:94691/1‑136 (MQ=255)
                                                                    cGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCCCCg                                                                           >  4:386213/1‑136 (MQ=255)
                                                                     gggTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                       <  1:100070/139‑1 (MQ=255)
                                                                       gTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGTCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATc                                                                        <  4:65228/136‑1 (MQ=255)
                                                                       gTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATc                                                                        <  3:68563/136‑1 (MQ=255)
                                                                          ccGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCtt                                                                  <  6:390395/139‑1 (MQ=255)
                                                                          cagatcTCGAATTAACTGGCCGTC‑TTTTT‑ACAAGGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCGTTGCAGCACATCCCCGTTTCGCCAGCTGGCGTATTAGCGAAGAGGCCCGCACCGATCGGCCtt                                                                  <  8:343882/134‑1 (MQ=255)
                                                                            gAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACAGGGAAAACCCTGGCGTTTCCCAACTTAATAGCCTTGCCGCACATCCCCCTTTCCCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGAGCGCCCGTcc                                                                >  8:400690/1‑139 (MQ=255)
                                                                             cgCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                               <  6:238059/138‑1 (MQ=255)
                                                                             aGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                               <  8:73006/139‑1 (MQ=255)
                                                                               cTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACAGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCCGCCGATCCCCCTTTCGCCAGCTGGCGTTATAGCGAAGAGGCCCGCACCGATCGCCCTTcccca                                                             >  7:250696/1‑137 (MQ=255)
                                                                                 cGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCa                                                              <  4:296388/136‑1 (MQ=255)
                                                                                       cACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcg                                                     >  7:275566/1‑139 (MQ=255)
                                                                                        aCTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                    <  2:306822/139‑1 (MQ=255)
                                                                                        aCTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTg                                                       <  3:189560/136‑1 (MQ=255)
                                                                                           ggCCGCC‑GTTTT‑CCACCGTCGGGACTGGTAAAACCCTGGCGTTCCCCAACATAATCGCCTGGCACCACATCCCCCTTGCGCCCGCTGGCGTAATCGCGACCAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGc                                                 <  2:198786/139‑1 (MQ=255)
                                                                                               gtc‑gtTTT‑ACACCGTCGTGACTGGGAAACCCCTGTCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCATTTCTCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTga                                             <  1:269259/139‑1 (MQ=255)
                                                                                                          cAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaat                                    <  7:141252/139‑1 (MQ=255)
                                                                                                             cgtcgtGACTGGGAAAACCCTGGCGTTACCCAACATAATCGCCTTGCAGCCCATCCCCCTTTCGCCAGCTGGCGGAATAGCGAAGAGGCCCGCCCCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaaaggcgaatggc                                 >  6:161827/1‑139 (MQ=255)
                                                                                                               tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGGGCAGCCTGAATGGCGAATGgcgc                               >  2:240009/1‑139 (MQ=255)
                                                                                                                  tGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAATAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCCGCCTGAATGGCGAAAGgcgc                               >  3:3723/1‑136 (MQ=255)
                                                                                                                      tgtgAAAACCCGGGCGTTACCCATCTTACTCGCCTTGCAGCACCTCCCCCTTGCGCCAGCTGGCGTAATTGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                        <  6:57126/136‑1 (MQ=255)
                                                                                                                       gggAAAACCCTGTCGTTCACCACCTTAATCGCCGTGCATCACATCCCCCTTTCGCCAGCTGTCGTCATAGCGAAGCGGCCCTCACCGATAGCCCTTCCCAACAGTTGCGCCGCCTGAAGGTCGAATGTCTCCTTATGCg                       <  6:209855/139‑1 (MQ=255)
                                                                                                                        ggAAAACCCTGGCGTTTCCCAACTTAATAGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGATGCCCGCACCGATAGCCCATGCCCACAGTTTCGCCGCCAGAATGGCGAATGGCGCCTGATGCgg                      >  8:28430/1‑139 (MQ=255)
                                                                                                                        ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                        <  5:176780/137‑1 (MQ=255)
                                                                                                                         gAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGAAGCCGGAATGGCGAATGGCGCCTCATGCGGt                     >  1:16945/1‑139 (MQ=255)
                                                                                                                              cccTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAt                   <  4:192627/136‑1 (MQ=255)
                                                                                                                                cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAttt                 >  4:266980/1‑136 (MQ=255)
                                                                                                                                cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct              <  7:198057/139‑1 (MQ=255)
                                                                                                                                  ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                        >  8:375252/1‑63 (MQ=255)
                                                                                                                                     gTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa         <  5:198500/139‑1 (MQ=255)
                                                                                                                                     gTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa         <  1:397940/139‑1 (MQ=255)
                                                                                                                                     gTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa         <  5:423076/139‑1 (MQ=255)
                                                                                                                                        aCCCAACTTAATCGCCTTGCAGCCCATCCCCCTTTCGCCAGCTGGCGGTATAGCGAAGAGGCCCGCACCGATCGCCCTGACCAACAGTTGCGCAGCCAGAATGGCGAAGGGCGCCTGATGCGGTATTTTCTACTTACg       >  6:281746/1‑138 (MQ=255)
                                                                                                                                        aCCCAACTTAATCGCCTTGCAGCACCTCCCCATTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg       <  1:304299/138‑1 (MQ=255)
                                                                                                                                        aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGTCGAATGGCGCCTGATGCGGTATTTTCTCCtaccg       >  8:256795/1‑134 (MQ=255)
                                                                                                                                        aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGAACGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCAGAAGCGGTATTTTCTCCATACg       >  1:210011/1‑138 (MQ=255)
                                                                                                                                        aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCGGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                       <  5:704/122‑1 (MQ=255)
                                                                                                                                          ccAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCAt    <  8:48204/139‑1 (MQ=255)
                                                                                                                                          ccAACTTAATCGCCTTGCAGCACATCCCCCTATCGCCAGCTGGCGTAATAGCGACGAGGCCCGCACCGATCGCCCTTCCCAACAGTAGCGCAGCCTGAATGGGGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCAt    <  8:310730/139‑1 (MQ=255)
                                                                                                                                           cAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg       >  3:402202/1‑135 (MQ=255)
                                                                                                                                            aaCTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcg                                       <  4:322831/102‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
CACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTC‑GTTTT‑ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCT  >  Exported/5874‑6150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑