Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 6,031 A→C 6.8% A48A (GCA→GCC)  lacZalpha → /vntifkey=4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported6,0310AC 6.8% 16870.6 / 214.4 6314A48A (GCA→GCC) lacZalpha/vntifkey=4
Reads supporting (aligned to +/- strand):  ref base A (2498/3255);  new base C (528/13);  total (3038/3276)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 7304 total aligned reads displayed.

CATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGC  >  Exported/5893‑6169
                                                                                                                                           |                                                                                                                                           
   gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCTTTTTACCAAGTCCTGACATGGAAAACCCTGTC‑GGTAAACAACTTAATAGCGTTGCAGCACAt                                                                                                                                          >  5:210580/1‑139 (MQ=255)
   gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACAGGGAAAACCTTGGC‑GTTTCCCCACTTAATGGCCGTGCAGcaca                                                                                                                                           >  1:421179/1‑138 (MQ=255)
    aTTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATc                                                                                                                                         <  1:347080/139‑1 (MQ=255)
        cGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATccccc                                                                                                                                     >  7:38048/1‑139 (MQ=255)
             aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GGTACCCCACATAATCGCCGTGCAGCACCTCCCCCcttgg                                                                                                                                >  6:98958/1‑137 (MQ=255)
             aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GGTACCCAACTTAATCGCCGTGCAGCACATCCCCCTTTCg                                                                                                                                >  7:349847/1‑139 (MQ=255)
               cTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTGAATCGCCTTGCAGCACTTCCCCCTTTCGcc                                                                                                                              >  6:114609/1‑139 (MQ=255)
                ttGCATGCCTGCAGGTCGACTCTAGAGGCTCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGGCGTGCCTGGGACAACCCGGGC‑GTTACCCAACTTAATCGCCGTGCCGCAGAGCCCCCGTTCg                                                                                                                                >  3:214257/1‑136 (MQ=255)
                ttGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GGTACCCCACGTAATCGCCTTGCAGCACATCCCCCATTCg                                                                                                                                >  4:268800/1‑136 (MQ=255)
                   cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                           <  5:186006/138‑1 (MQ=255)
                   cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                           <  5:79654/138‑1 (MQ=255)
                   cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                           <  1:84035/138‑1 (MQ=255)
                           cAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTAAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGt                                                                                                                     <  3:119741/136‑1 (MQ=255)
                            atgTCGAATATAGATGATCCACTGGTACAGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCTCCTTGCAGAACATTCCCCTTTCTCCAGCTGGCGTa                                                                                                                    <  4:165888/134‑1 (MQ=255)
                            aGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTa                                                                                                                    <  3:286758/136‑1 (MQ=255)
                                 gACTCTAGAGGATCCCCGGGTACCGAGCTCTAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                            <  5:161311/139‑1 (MQ=255)
                                 gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCTGCACATCCCCCTTTCGCCTGCTGGCGTAATAGCGaa                                                                                                            <  6:395143/139‑1 (MQ=255)
                                  aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGAAAACCCTGGC‑GTTACCCAACTTAATAGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCg                                                                                                              >  2:88703/1‑136 (MQ=255)
                                  aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAag                                                                                                           <  7:82011/139‑1 (MQ=255)
                                  aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAag                                                                                                           >  2:43067/1‑139 (MQ=255)
                                  aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAAACGCCTTGCAGCACATCCCCCTTTCCCCAGCTGGCGTAATAGCg                                                                                                              >  6:367478/1‑136 (MQ=255)
                                    tctAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCGGGCGTAATAGCg                                                                                                              >  3:395980/1‑134 (MQ=255)
                                     ctAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATAGCCATGCAGCACATCCCCCTTTCGCCACCAGGCGTAATAGCg                                                                                                              >  4:192914/1‑133 (MQ=255)
                                      tAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACCACGTCGTGACTGGGCAAACCCGGGC‑GTTACCCCACGTAATCTCCGTGGCGCCCAACCCCCGTTCGCCCGCCTGCGGAATAGCGCAGA‑GGc                                                                                                      >  6:294177/1‑139 (MQ=255)
                                        gagGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGc                                                                                                      <  7:28393/137‑1 (MQ=255)
                                         agGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCg                                                                                                   <  1:29268/139‑1 (MQ=255)
                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTCAACGTCGTGGCTGGGGAAACCCTGGG‑GGTACCCCACGTAATCGCCTTGGCGCCCATCCCCCTTTCGCCCGCGGGCGGAATAGCGAAGA‑GGCCCGcccc                                                                                               >  8:169059/1‑139 (MQ=255)
                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGTAAACCCTGGC‑GGTTCCCAACTTAATCGCCTTGCAGCACATCCCCCATTAGCCCGCAGGCGGAATATCGAAGA‑GGcccccccc                                                                                               >  1:165955/1‑134 (MQ=255)
                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCCGCTGGCGTAATAGCGAAGA‑GGCCCGCAcc                                                                                               >  5:401460/1‑139 (MQ=255)
                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCg                                                                                                   <  7:54395/135‑1 (MQ=255)
                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCAcc                                                                                               >  6:165376/1‑139 (MQ=255)
                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCGGGCGGAATAGCGAAGA‑GGCCCGcccc                                                                                               >  8:225141/1‑139 (MQ=255)
                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACCTCGTGACTGGGAAAACACAAGA‑GATAAACAACAAAAAAGCCTTGAAGACCCTCCCCCATTCCACACCAGGCGAAATACCAAAAA‑AGCCCCCAcc                                                                                               >  6:14101/1‑139 (MQ=255)
                                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACCTCGTGACAGGGAAAACCCGGTCGGTAACCCAAGTAAAAC‑CCCTGAACCACATCCCCCAATAGCCACCACGCGGAATAACGAAAA‑GGcccccccc                                                                                               >  8:51970/1‑134 (MQ=255)
                                              ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCAcc                                                                                               >  8:347555/1‑138 (MQ=255)
                                              ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGCCAGGGGAAACCCTGGC‑GGTACCCAACTTAAAACCCTTTCCGCACAACCCCCGTTAGCCAGCGGGGGGAATAACGACGAGGGCCCGcccc                                                                                               >  5:375462/1‑139 (MQ=255)
                                                ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCAcc                                                                                               >  3:186289/1‑136 (MQ=255)
                                                 cGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCCACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCCCCg                                                                                              >  3:159802/1‑136 (MQ=255)
                                                 cGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGGGCCTGGGAAAACCCGGGC‑GTTTCCCAACTTAATAGCCTTGCAGCCCCTCCCCCGTTCGCCAGCGGGGGTAAAATCGAAGA‑GGCCCGccccc                                                                                              >  3:315414/1‑135 (MQ=255)
                                                  gggTACCGAGCTCGACTTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGACACCCGTGGC‑GGTACCAAAGTTAATCGCCTTGCAGCACATCCCCCTTTGGCCAGCTGGCGTAATAGCGATGA‑GGCCCGCACCGATCg                                                                                          <  8:198385/139‑1 (MQ=255)
                                                     tACCGAGCTCGAATTAACTGGCCTGCGTTTTACATCGTCGTGACTTGTAAAACCCTGGC‑GTTACCCAACTTTCTCGGCTTGCTGCACATAACCCTTTCGCCAGCTGGCTTAATAGCGAAGA‑GGCCCGCAGCGATCGccc                                                                                       <  8:310045/139‑1 (MQ=255)
                                                     aaCCGAGCACGAATTCACTGGCCGCCGTTTTACAACGTCGTGACTGGGAAAACCCAGGC‑GTTACCCACCTTAATCTCCTTGCAGCACATCCCCCTTTCGCCCGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCg                                                                                          <  4:79394/135‑1 (MQ=255)
                                                        cGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTTCAGCCCCTCCCCCTTTCGCCAGCTGGCGGAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTc                                                                                    >  8:132831/1‑139 (MQ=255)
                                                         gAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGTCTGGGTAAAACCAGGC‑GTTACCCCACTTAATACCCGTGCAGCCCATACCCCATTAGCCCGCGGGCGTGATATCGAAGA‑CGCCCCCCCCGAACGcccggcc                                                                                   >  2:116478/1‑135 (MQ=255)
                                                          aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGAAAACCCTGGC‑GTTACCCAACTTAATAGCCTTGCAGCCCATCCCCCGTTAGCCAGCTGGCGTTATATCGCAGA‑GGCCCCCACCGATCGcccagcc                                                                                   >  7:234405/1‑134 (MQ=255)
                                                          aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTccc                                                                                  <  7:122283/139‑1 (MQ=255)
                                                          aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTccc                                                                                  >  2:65816/1‑139 (MQ=255)
                                                          aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCGGGC‑GTTTCCCAACATAATCGCCTTTCAGCACCTCCCCCGTTCGCCCGCTGGCGTAATATCGAAGA‑GGCCCGCACCGATCGCCCGTccc                                                                                  >  2:242830/1‑139 (MQ=255)
                                                             tCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGcac                                                                                                                                            <  4:334235/79‑1 (MQ=255)
                                                               gAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATGGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACAGATCGCCCTTCCCAACa                                                                              <  1:12947/138‑1 (MQ=255)
                                                                     aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                       <  1:321689/139‑1 (MQ=255)
                                                                     aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                       <  2:306822/139‑1 (MQ=255)
                                                                     aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTg                                                                          <  4:301004/136‑1 (MQ=255)
                                                                     aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAAACCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCATTCGCCCGCTGGCGGTATAGCGAAGA‑CGCCCCCCCCGCTCGCCCGTCCCACCAGTTgcgc                                                                       >  6:69409/1‑139 (MQ=255)
                                                                        ggCCGGCGTTTTACTACGGGGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCTGCACATCCCCTTTTCGCCAGGTGGCGTAATAGCGAAGA‑GGCCCGGACCGATCGCCCTTCCCAACAGTTgcgg                                                                       <  4:362982/136‑2 (MQ=255)
                                                                            gtcgtTTTACACCGGCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTAAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATACCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTga                                                                <  1:232676/139‑1 (MQ=255)
                                                                            gtcgtTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTga                                                                <  1:62051/139‑1 (MQ=255)
                                                                                        cgtcgtGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACCTCCCCCTTTCGCCAGCTGGCGGAATAGCGAAGC‑GGCCCGCCCCGCTCGCCCGTGCCCCCAGTTGCGCCGCCTgcagggggaatggg                                                    >  8:110128/1‑138 (MQ=255)
                                                                                          tcgtGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGgcgc                                                  >  2:21204/1‑139 (MQ=255)
                                                                                          tcgtGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCACCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGgcgc                                                  >  8:252163/1‑139 (MQ=255)
                                                                                           cgtGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATACCCCGTCCCAACAGTTGCGCAGCCTgaaaggcgaatggc                                                    >  4:397694/1‑136 (MQ=255)
                                                                                             tGACTGGGAAACCCCTGGC‑GTTTCCCAACTTAATCGCCTTGCCGCACATCCCCCTTTCGCCCGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTg                                               >  6:189965/1‑139 (MQ=255)
                                                                                             tGACTGGGAAAACCCTGGC‑GTTACCCAACTTATTCGCCTGGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGACGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTg                                               <  1:383262/139‑1 (MQ=255)
                                                                                               aCTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCt                                                <  3:337934/136‑1 (MQ=255)
                                                                                                  gggAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                                          <  7:148714/139‑1 (MQ=255)
                                                                                                   ggAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACTTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                                          <  7:260222/138‑1 (MQ=255)
                                                                                                    gAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                       >  7:177561/1‑108 (MQ=255)
                                                                                                    gAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGACGCAGATACCCCTTTCGCCAGCTGGCGTAATATCGAAGA‑GTCCCGCACCGAAAGCCCTTCCCAACATTTGCGCAGCCAGAATGGCGAAAGGCTCCTGATGCGGt                                        >  8:402025/1‑139 (MQ=255)
                                                                                                     aaaaCCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACCTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                                          >  4:121634/1‑136 (MQ=255)
                                                                                                       aaCCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATACCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCgg                                         >  3:247581/1‑135 (MQ=255)
                                                                                                           cgggC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATCGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTGGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct                                 <  8:282042/137‑1 (MQ=255)
                                                                                                           cTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCCCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAttt                                    <  4:43522/136‑1 (MQ=255)
                                                                                                             ggC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCCCCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTcc                               >  7:314042/1‑139 (MQ=255)
                                                                                                                    aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATa                                                                                                                 <  7:20698/52‑1 (MQ=255)
                                                                                                                    aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGcc                                                                                        <  5:312740/76‑1 (MQ=255)
                                                                                                                    aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTGCg                          >  5:238199/1‑138 (MQ=255)
                                                                                                                    aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                          <  5:107540/138‑1 (MQ=255)
                                                                                                                    aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCACCTGATGCGGTATTTTCTCCTTACg                          >  2:337288/1‑138 (MQ=255)
                                                                                                                     cccAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                           >  6:217337/1‑41 (MQ=255)
                                                                                                                      ccAACTTACTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCAt                       <  1:264902/139‑1 (MQ=255)
                                                                                                                       cAACTTAATCGCCTTGCAGCACATCCCCCTTTCGcc                                                                                                                              >  4:228552/1‑36 (MQ=255)
                                                                                                                        aaCTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCACAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCa                        <  3:425183/136‑1 (MQ=255)
                                                                                                                          cTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCtt                                                                                     <  8:377738/73‑1 (MQ=255)
                                                                                                                             aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAAGAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  7:40803/137‑1 (MQ=255)
                                                                                                                             aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                  <  2:370929/137‑1 (MQ=255)
                                                                                                                             aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgt                   >  3:364437/1‑136 (MQ=255)
                                                                                                                             aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCt                     <  3:66523/134‑1 (MQ=255)
                                                                                                                              aTCGCCTTGCAGCACCTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCg                <  2:271061/138‑1 (MQ=255)
                                                                                                                               tCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaatg                                                      >  5:132677/1‑99 (MQ=255)
                                                                                                                                 gCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTAt            <  1:100021/139‑1 (MQ=255)
                                                                                                                                    ttGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTCttt          >  5:448/1‑138 (MQ=255)
                                                                                                                                      gccgcaCATCCCCCTTTCGCCCGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  1:198047/136‑1 (MQ=255)
                                                                                                                                      gcagcaCATCCCCCTATCGCCAGCTGGCGTAAAAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGATGCGCAGCCTGAATGGCTAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  6:421339/139‑1 (MQ=255)
                                                                                                                                       cagcaCATCCCCCTTTCGCCCGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  5:348168/138‑1 (MQ=255)
                                                                                                                                       cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  2:162431/138‑1 (MQ=255)
                                                                                                                                       cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  6:251203/138‑1 (MQ=255)
                                                                                                                                       cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  6:328596/138‑1 (MQ=255)
                                                                                                                                       cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac       <  5:275131/138‑1 (MQ=255)
                                                                                                                                        agcaCCTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGGTGCGCAGCCTGAATGGCGAATGGCGCCTGAGGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCacac     <  8:72249/139‑1 (MQ=255)
                                                                                                                                           acaTCCCCCTTTCGCCAGCTGGCGTATTAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAAGGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCacac     <  3:92372/136‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC‑GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGA‑GGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGC  >  Exported/5893‑6169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑