BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,033	A→C	5.0%	H49P (CAT→CCT)	lacZalpha →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,033	0	A	C	5.0%	18999.9 / 136.4	7059	H49P (CAT→CCT)	lacZalpha	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (2622/3917); new base C (456/40); total (3082/3977)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 7371 total aligned reads displayed.

TGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCAT  >  Exported/5895‑6171

 gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACAGGGAAAACCCAGGCGTTACCCAACATAATCGCCTTGCACCCCAt                                                                                                                                           >  7:180140/1‑139 (MQ=255)

  aTTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATc                                                                                                                                          <  1:345664/139‑1 (MQ=255)

           aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTAGTGACTGGGAAAACCCAGGCGTGACCCACCGTAAACGCCGTGCAGCCCATCCCCCattag                                                                                                                                 >  2:305367/1‑137 (MQ=255)

            gCTTGCATCCCTGCAGGTCGACTCTACAGGATCCCCGGGTACCGAGCACGAATTCACAGGCCGACGTATATCAACGACGTAACTGGGTAAACCCTAGCGTTTCCCAACATAAACCCCTTCCAGCACATcccccaatcac                                                                                                                                >  8:252924/1‑133 (MQ=255)

                gCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCa                                                                                                                              <  6:34147/137‑1 (MQ=255)

                 cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                            <  5:219077/138‑1 (MQ=255)

                 cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                            <  1:83644/138‑1 (MQ=255)

                     ccTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCg                                                                                                                       <  2:336295/139‑1 (MQ=255)

                     ccTGCAGGTCGACTCTAGAGGAGCCCCGGGTACCGAGCTCGAATTCACTGGCCGGCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCg                                                                                                                       <  5:251729/139‑1 (MQ=255)

                       tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGCGCTCGAATTCACTGGCCGTCGTGTTTCAACGTCGTGCCTGGGAAAACCCTGGCGGTACCCAACATAATCGCCGTGCAGCACCTCCCCCTTTCGCCCGCGGGCGt                                                                                                                      >  6:122156/1‑138 (MQ=255)

                               gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCCGCTGGCGTAATAGCGaa                                                                                                             <  2:159801/139‑1 (MQ=255)

                               gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGc                                                                                                                <  4:375596/136‑1 (MQ=255)

                               gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGc                                                                                                                <  3:69528/136‑1 (MQ=255)

                               gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                             <  6:74858/139‑1 (MQ=255)

                               gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                             <  8:377821/139‑1 (MQ=255)

                                           tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGCAAACCCAGGCGGTACCCAACATAATAGCCTTGCCGCCCCTACCCCCTTCGCCCCCGGGCGGAATAGCGAAGAGGcccccccc                                                                                                 >  1:165858/1‑134 (MQ=255)

                                           tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCCGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAcc                                                                                                 >  1:6073/1‑139 (MQ=255)

                                           tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGCGTTACCCCACATAATCGCCGTGCAGCACCTCCCCCTTTACCCCGCAGGCGGAATAGCGAAGAGGCCCGcccc                                                                                                 >  5:292473/1‑139 (MQ=255)

                                           tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAATGGGAAAACCCTGGCGTTACCCCACGTAATCGCCGTGCAGCACCTACCCCATTAGCCACCTGGCGTAATACCGAAGCGGCCCGcccc                                                                                                 >  8:50643/1‑139 (MQ=255)

                                           tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTAAAAACTCGTGTCTGGGGAAACCCAGGCGTAAACCCAGATAATAGCCTTTCCGCCCAACCCCCTGTCCCCAACAGACGTAAATGAGAACAGGCCCGCAcc                                                                                                 >  6:262989/1‑139 (MQ=255)

                                            ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACATAATCGCCTTGCAGCACATCCCCCTTTAGCCAGCTGGCGTAAAAGCGAAGAGGCCCGCCCCg                                                                                                >  8:11196/1‑139 (MQ=255)

                                             cccGGGTACCGAGCGCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAAATTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGa                                                                                               <  2:211016/139‑1 (MQ=255)

                                              ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCCACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAcc                                                                                                 >  3:116883/1‑136 (MQ=255)

                                              ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAcc                                                                                                 >  3:366219/1‑136 (MQ=255)

                                              ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATAGCCTTGCAGCCCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAcc                                                                                                 >  3:314083/1‑136 (MQ=255)

                                              ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTGAACCCACTTAATCGCCGTGCAGCCCATCCCCCATTAGCCAGCTGGCGTTATATCAGAGAGGCCCCCAcc                                                                                                 >  4:279353/1‑136 (MQ=255)

                                              ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACCTAGTGACTTGGTAAAACCTGGCGGTACCCAACCTAATCGCCATGCAGCACAACCCCCATTAGCCACCGGGGGGAATAGCCAAGAGGCCCCCAcc                                                                                                 >  4:171053/1‑136 (MQ=255)

                                              ccGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGGCTGGGGAAACCCAGGCGGTACCCCACGTTATAGCCCTGGCGCACAACCCCCGTTCGCCCGCGGGCGGAATAGGGCACAGGCCCGcccc                                                                                                 >  4:62505/1‑136 (MQ=255)

                                                 ggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTTCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGAt                                                                                              >  4:410108/1‑136 (MQ=255)

                                                     ccGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCCCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCtt                                                                                       >  2:2410/1‑139 (MQ=255)

                                                     ccGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCGTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                                                          <  4:92858/136‑1 (MQ=255)

                                                     ccGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTAAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCtt                                                                                       <  5:144636/139‑1 (MQ=255)

                                                     ccGAGCTCGAATTCACTGGCCGTCGTTTTAAAACGTCCTGACTGGGAAAACCCTTGCCTTAACCAACATAAAAGCCATGCCGAACATCCCCCATTCGCCAGCTGTCGGTAAACCGAAGCTGCCCCCACCGAACccccga                                                                                       >  6:174053/1‑137 (MQ=255)

                                                        aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                                                    <  7:411509/139‑1 (MQ=255)

                                                        aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                                                    <  1:28923/139‑1 (MQ=255)

                                                        aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                                                    <  7:102631/139‑1 (MQ=255)

                                                           tCGAATTCACTGGCCGTCGTTTTACAACGTCGTGGCTGGGAAAACCCTGGCGTTACCCCACTTAATCGCCGTGGCGCACATCCCCCATTCGCCCGCTGGGGGAATAGCGAAGAGGCCCGCCCCGCTCGcccagccc                                                                                    >  4:241518/1‑131 (MQ=255)

                                                             gAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATGGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACAGATCGCCCTTCCCAACa                                                                                <  1:12947/138‑1 (MQ=255)

                                                             gAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACa                                                                                <  8:22573/138‑1 (MQ=255)

                                                                   aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                         <  5:172224/139‑1 (MQ=255)

                                                                             gtTTAACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatg                                                               <  6:401317/139‑1 (MQ=255)

                                                                                    aaCGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaatg                                                        <  8:137768/139‑1 (MQ=255)

                                                                                      cgtcgtGACTGGGAAAACCCTTGCGTTACCCAACTTAATCGCCATGCAGCACATACCCCATTCCCCAGCTGGCCTAATAGCGAAGAGGCCCGCACCGAAATCCCTTACCAACAGTTTCCCAGCCTgaatggcgaaagcc                                                      >  5:197085/1‑135 (MQ=255)

                                                                                      cgtcgtGACTGGGAAAACCCTGGCGTTACCCAACATAATCGCCTTGCAGCACATCCCCCTTTAGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCCCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaatggc                                                      >  8:312256/1‑139 (MQ=255)

                                                                                       gtcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGaatggcgaatggcg                                                     >  6:198057/1‑139 (MQ=255)

                                                                                        tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTTATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGgcgc                                                    >  5:8777/1‑139 (MQ=255)

                                                                                        tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGgcgc                                                    >  1:197088/1‑139 (MQ=255)

                                                                                            gACTGGGAAAACCCTGGCGTTTCCCAACTAAATAGCCTTGCAGCACATGCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCCCTCCACCTTACCAACAGTTGCGCAGCCAGAATGGCGTATTgcgc                                                    >  4:342583/1‑135 (MQ=255)

                                                                                             aCTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCt                                                  <  3:212582/136‑1 (MQ=255)

                                                                                                 ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGa                                                <  3:184670/134‑1 (MQ=255)

                                                                                                 ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                                             <  6:303814/137‑1 (MQ=255)

                                                                                                 ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                                             <  5:326897/137‑1 (MQ=255)

                                                                                                 ggAAAACCCTGGCGTTACCCAACATAATCGCCTTTCAGCCCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCCCCGATCGCCCGTCCCAACACTTGCGCAGCCGGAATGGCGAATGGCGCCGGATGCgg                                           >  7:84954/1‑139 (MQ=255)

                                                                                                 ggAAAACCCTGGAGTTAACCAACTTAATCGCGTTTCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGTGAATGGCGGCTGATGc                                             <  1:232004/137‑1 (MQ=255)

                                                                                                  gAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCgg                                           >  5:357747/1‑138 (MQ=255)

                                                                                                  gAAAACCCTGGCGTGTCCCCACTTAATAGCCTTGCAGCACATCCCCCTTTCGCCCGCGGGCGTAATAGCGAAGAGGCCCCCACCGATCGCCCGTCCCCCCAGTTGCGCCGCCAGCAAGGCGCATGGCGCCTGATGCGGt                                          >  2:425051/1‑139 (MQ=255)

                                                                                                     aaCCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGt                                          <  3:338726/136‑1 (MQ=255)

                                                                                                     aaCCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtt                                       <  2:11461/139‑1 (MQ=255)

                                                                                                       cccTGTCGGTACCCAACTTAATCGCATTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCTCCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt                                     <  6:14654/139‑1 (MQ=255)

                                                                                                           ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTcc                                 >  1:319240/1‑139 (MQ=255)

                                                                                                           ggCGTTACCCAACTTAATCGCCATGCAGCACATCCCCCTATCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCATCAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTcc                                 <  6:223045/139‑1 (MQ=255)

                                                                                                            gagTTACCCAACTTAATCGACTTGAAGCAAATCCCCCTTTCGCCATCGTTCGTAATATCGAAGAGGACCGCACCGATCGCCCTTCCCAACAGTTGAGAAGCATGAATGGCTAATGGCGCCTGATGCGGTATTTTCTcc                                 <  7:318490/136‑1 (MQ=255)

                                                                                                            gCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct                                   >  3:96732/1‑136 (MQ=255)

                                                                                                              gTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa                              <  2:268535/139‑1 (MQ=255)

                                                                                                              gTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa                              <  7:263786/139‑1 (MQ=255)

                                                                                                                 ccccAACTTAAACGCCTTGCAGCACATCCCCGTTTCGCAAGCTGGCGTAAAAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                            <  7:385844/137‑1 (MQ=255)

                                                                                                                 aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                                            <  7:153208/74‑1 (MQ=255)

                                                                                                                 aCCCAACTTAATCGCCTGGCAGCACAGCCCCCTTTCGCCCGCTGGCGTAATCGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                            <  5:117957/138‑1 (MQ=255)

                                                                                                                  cccAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                                             <  8:347799/120‑1 (MQ=255)

                                                                                                                   ccAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCAt                         <  8:172820/139‑1 (MQ=255)

                                                                                                                   ccAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCAt                         <  7:21025/139‑1 (MQ=255)

                                                                                                                   ccAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCAt                         <  1:419275/139‑1 (MQ=255)

                                                                                                                       cTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgt                     >  7:125867/1‑139 (MQ=255)

                                                                                                                        ttAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                    <  6:374734/139‑1 (MQ=255)

                                                                                                                          actCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCATACGCATCTGTGCg                  <  1:260852/137‑1 (MQ=255)

                                                                                                                          aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                    <  7:235953/137‑1 (MQ=255)

                                                                                                                          aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGAGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                    <  5:411510/137‑1 (MQ=255)

                                                                                                                           aTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgt                     <  3:398992/135‑1 (MQ=255)

                                                                                                                           aTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCg                  <  7:286132/138‑1 (MQ=255)

                                                                                                                           aTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCg                  <  8:401904/138‑1 (MQ=255)

                                                                                                                             cGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCt                       >  3:426981/1‑131 (MQ=255)

                                                                                                                              gCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTAt              <  1:100021/139‑1 (MQ=255)

                                                                                                                               ccTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCgg                 >  3:283539/1‑135 (MQ=255)

                                                                                                                                 ttGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTAttt            >  5:385196/1‑138 (MQ=255)

                                                                                                                                   gcagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCTGTATTTTCTCCTTACGCATCTGTGCGGTATTTca          <  8:71410/138‑1 (MQ=255)

                                                                                                                                   gcagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  7:55105/139‑1 (MQ=255)

                                                                                                                                   gcagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  5:53678/139‑1 (MQ=255)

                                                                                                                                    cagctCATCCCCCTTTCGCCAGCTGGCTTAAAAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTTAATGGCGAATGTCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTAttt            <  4:306703/135‑1 (MQ=255)

                                                                                                                                    cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTAttt            <  3:152278/135‑1 (MQ=255)

                                                                                                                                    cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTAttt            <  3:246319/135‑1 (MQ=255)

                                                                                                                                    cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTAttt            <  4:132115/135‑1 (MQ=255)

                                                                                                                                    cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTAttt            <  4:199319/135‑1 (MQ=255)

                                                                                                                                    cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  7:353615/138‑1 (MQ=255)

                                                                                                                                    cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  6:427770/138‑1 (MQ=255)

                                                                                                                                    cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  2:86170/138‑1 (MQ=255)

                                                                                                                                    cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  8:282650/138‑1 (MQ=255)

                                                                                                                                    cagcaCATCCCCCCTCCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGAGCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  2:63956/138‑1 (MQ=255)

                                                                                                                                        acaTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGc    <  1:380696/139‑1 (MQ=255)

                                                                                                                                         caTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTATCCTTACGCAGATTTGCGTTATTTCACACCGc    >  2:367799/1‑138 (MQ=255)

                                                                                                                                          aTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCat  <  8:201734/139‑1 (MQ=255)

TGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCAT  >  Exported/5895‑6171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`