BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,047	A→C	5.5%	S54R (AGC→CGC)	lacZalpha →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,047	0	A	C	5.5%	20478.6 / 190.1	7931	S54R (AGC→CGC)	lacZalpha	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (2574/4725); new base C (487/99); total (3064/4867)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 7957 total aligned reads displayed.

TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCA  >  Exported/5909‑6185

  gCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                                          <  2:333832/139‑1 (MQ=255)

   cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                                          <  1:166216/138‑1 (MQ=255)

   cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                                          <  1:381900/138‑1 (MQ=255)

   cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                                          <  1:84035/138‑1 (MQ=255)

                 gcctctAGAGGAGCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGac                                                                                                                           <  1:417954/137‑2 (MQ=255)

                 gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGc                                                                                                                              <  3:181507/136‑1 (MQ=255)

                 gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                                           <  6:251192/139‑1 (MQ=255)

                 gACTCTAGAGGATCCCCGGGGACCGAGCTCTAATTCACTGGCCGTCGTTTTACACCGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                                           <  5:136060/139‑1 (MQ=255)

                  aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAag                                                                                                                          <  7:82011/139‑1 (MQ=255)

                  aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGCGTTACCCAACATAATCGCCTTGCCGCCCATCCCCCATAAGCCCGCTGGCGTAATAGCg                                                                                                                             >  6:289757/1‑136 (MQ=255)

                   ctctAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                                                           <  7:379394/137‑1 (MQ=255)

                   ctctAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCCTTACCCAACTTAATCGCCTTGCAGCACCTCCCCCTTTCGCCCGCTGGCGTAATAGCGaa                                                                                                                           >  7:22917/1‑137 (MQ=255)

                            aTCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTCAACGTCGTGACTGGGAAAAACCTGGCGTTTCCCCACGTAATCGCCTTGCCGCCCATCCCCCGTTCGCCCGCGGGCGGAATAGCGAAGAGGGCCGccc                                                                                                                >  1:348213/1‑137 (MQ=255)

                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCGGGGCAAACCCGGGCGGTTCCCCACATAATCGCCATGCCGCACGTCCCCCGTTCGCCCGCGGGCGGAATATCGACCGGGCCCGcccg                                                                                                               >  2:363502/1‑136 (MQ=255)

                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAAAATAATCGCCTTGCAGCCCATCCCCCGTTCGCCCGCTGGCGGAATATCGAAGAGGCCCGCAcc                                                                                                               >  8:136499/1‑139 (MQ=255)

                             tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGCGTTACCCAACATAATAGCCTTGCAGCCCAACCCCCATTAGCCACCTGGCGGAATATCCAAGATGcccagtg                                                                                                                >  6:9166/1‑134 (MQ=255)

                              ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGGCGTGACGGGGGAAACCCAGGCGTTTCCCAACATAATCCCCGGTCCGCCCCTACCCCAGTACCCACCGGGCGGAATAGCGCAGAGGCCCCCCCCg                                                                                                              >  8:371362/1‑139 (MQ=255)

                               cccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCGGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTTATAGCGACGAGGCCCGCACCGa                                                                                                             <  8:196735/139‑1 (MQ=255)

                                ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAcc                                                                                                               >  3:366219/1‑136 (MQ=255)

                                  gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGa                                                                                                             <  4:51625/136‑1 (MQ=255)

                                  gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                                                          <  7:17449/139‑1 (MQ=255)

                                  gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                                                          <  8:72569/139‑1 (MQ=255)

                                  gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCg                                                                                                          <  6:216054/139‑1 (MQ=255)

                                    gTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGcc                                                                                                        <  1:199717/139‑1 (MQ=255)

                                     tACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGa                                                                                                             >  3:110596/1‑133 (MQ=255)

                                          aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGTCTGGGTAAAACCGGGCGGTACCCAACATAATAGCCGTGCAGCCCCTCCCCCATTAGCCCGCAGGCGGAAAAGCCAAAAGGCCCGCCCCCCGCGCCCGTccc                                                                                                  >  2:301807/1‑139 (MQ=255)

                                          aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCATGCAGCACCTACCCCGTTAGCCAGCAGGCGTAATAGCGAAGACGCCCCCACCGCACCCCCATccc                                                                                                  >  8:11060/1‑139 (MQ=255)

                                          aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATAGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                                                                  >  1:287820/1‑139 (MQ=255)

                                          aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACATAATCGCCTTGCAGCCCATACCCCATTCGCCCCCAGGCGTAATAGCGAAGAGGCCCGCCCCCATCGCCCtt                                                                                                     >  4:338726/1‑136 (MQ=255)

                                             tCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCGGGCGTTTCCCAACTTAATCGCCTTGCAGCCCCTCCCCCTTTCGCCGGGGGGGGGAGTAGGGATGCGGCCCGCCGCGGTCGCGCGTccc                                                                                                  >  3:311961/1‑136 (MQ=255)

                                                       tGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAg                                                                                     >  5:186566/1‑139 (MQ=255)

                                                         gCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                                       <  3:398724/135‑1 (MQ=255)

                                                            gtcttTTTACAACGCCGGGACTGGCAAAACCCGGGGGTTACCCAACTTAATCGCCTTGCAGCACAACCCCCTTTCGCCAGCTGGCGTAATAGCTACGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTga                                                                                <  5:271381/139‑1 (MQ=255)

                                                            gtcgtTTTACAACGTCGTGACTGGGAAAACCCAGGCGTTTCCCAACTTAATAGCCTTTCAGCACATCCCCCTTTAGCCAGCTGGCGTAATAGCGAAGAGGCCCGCCCCGCTCGCCCTTGCCAACAGTTTCGCAGcc                                                                                   >  4:167528/1‑136 (MQ=255)

                                                                        cgtcgtGACTGGGAAAACCCTGGCGTTACCCAACATAATCGCCATGCAGCACCTCCCCCTTTCCCCACCTCGCGTAATAGCGAAGAGGCCCCCACCCATCCCCCATCCCAACACTTGCGCAGCCAgaatggcgaaaggc                                                                    >  6:112629/1‑139 (MQ=255)

                                                                          tcgtGACTGGGAAAACCCTGGCGTTACCCACCTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaatgg                                                                     <  4:269601/136‑1 (MQ=255)

                                                                          tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCCACAGTTGCGCAGCCTGAATGGCGAATGgcgc                                                                  >  7:30899/1‑139 (MQ=255)

                                                                           cgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                                          >  5:308785/1‑66 (MQ=255)

                                                                             tGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGgcgc                                                                  >  3:95687/1‑136 (MQ=255)

                                                                               aCTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCt                                                                <  3:337934/136‑1 (MQ=255)

                                                                               aCTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCt                                                                <  3:212582/136‑1 (MQ=255)

                                                                                   ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGa                                                              <  3:69341/134‑1 (MQ=255)

                                                                                   ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                                                           <  6:386816/137‑1 (MQ=255)

                                                                                   ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                                                           <  5:211272/137‑1 (MQ=255)

                                                                                     aaaaCCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTa                                                       <  2:2025/139‑1 (MQ=255)

                                                                                       aaCCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtt                                                     <  2:11461/139‑1 (MQ=255)

                                                                                       aaCCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtt                                                     <  8:307871/139‑1 (MQ=255)

                                                                                         cccTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt                                                   <  6:166162/139‑1 (MQ=255)

                                                                                           cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAttt                                                    <  3:281026/136‑1 (MQ=255)

                                                                                             ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTcc                                               >  1:319240/1‑139 (MQ=255)

                                                                                              gCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTcc                                               <  7:203709/138‑1 (MQ=255)

                                                                                                   aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTga                                                                                >  1:61510/1‑100 (MQ=255)

                                                                                                   aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa                                            <  4:233845/136‑1 (MQ=255)

                                                                                                   aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                                          >  7:52595/1‑138 (MQ=255)

                                                                                                   aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                                          >  7:347381/1‑138 (MQ=255)

                                                                                                     ccAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCAt                                       <  8:48204/139‑1 (MQ=255)

                                                                                                      cAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTg                                                                                          >  3:425945/1‑87 (MQ=255)

                                                                                                            actCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCATACGCATCTGTGCg                                <  1:260852/137‑1 (MQ=255)

                                                                                                            aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                                  <  7:406031/137‑1 (MQ=255)

                                                                                                            aaTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCt                                     <  3:14928/134‑1 (MQ=255)

                                                                                                             aTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCg                                <  7:117251/138‑1 (MQ=255)

                                                                                                             aTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCg                                <  5:162824/138‑1 (MQ=255)

                                                                                                              tCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCgg                               >  5:77799/1‑138 (MQ=255)

                                                                                                              tCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCgg                               >  2:408849/1‑138 (MQ=255)

                                                                                                                gCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTAt                            <  1:100021/139‑1 (MQ=255)

                                                                                                                 ccTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACa                                                                                              >  7:145749/1‑72 (MQ=255)

                                                                                                                   ttGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACACTTTCGCAGCCTGAATGGCGAATGGCGCCTGAAGCGGAATTTTCTCCTAACGCATCTGTGCGGTAttt                          >  8:399817/1‑138 (MQ=255)

                                                                                                                      cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTAttt                          <  3:246319/135‑1 (MQ=255)

                                                                                                                      cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTAttt                          <  4:127166/135‑1 (MQ=255)

                                                                                                                      cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac                       <  1:131675/138‑1 (MQ=255)

                                                                                                                      cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac                       <  8:340498/138‑1 (MQ=255)

                                                                                                                      cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac                       <  8:280440/138‑1 (MQ=255)

                                                                                                                      cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac                       <  8:250113/138‑1 (MQ=255)

                                                                                                                      cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac                       <  2:26649/138‑1 (MQ=255)

                                                                                                                      cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac                       <  5:109862/138‑1 (MQ=255)

                                                                                                                      cagcaCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac                       <  6:326208/138‑1 (MQ=255)

                                                                                                                           caTCCCCCTTTCGCCCGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac                       <  1:232705/133‑1 (MQ=255)

                                                                                                                           caTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGc                  >  6:142236/1‑138 (MQ=255)

                                                                                                                           caTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACAACGc                  >  6:57057/1‑138 (MQ=255)

                                                                                                                           caTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTCAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGc                  >  6:189470/1‑138 (MQ=255)

                                                                                                                            aTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCat                <  8:22200/139‑1 (MQ=255)

                                                                                                                            aTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCa                 >  2:157246/1‑138 (MQ=255)

                                                                                                                            aTCCCCCTTTCGCCAGATGGCGTAATAGCGAATAGTCCCGCACCGATCGCCCATCCCAACAGTTTCGCAGCCTGAATGGCGAATGGCGCCTGATGACGTAGTTTCTCCTTAAGCATCTGTGCGGTATTTAACACCGCa                 >  2:238989/1‑138 (MQ=255)

                                                                                                                              cccccTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCTAATGGCGCCTGATGCGGTATTTTCTCATTACGCATCTGTGCGGTATTTCACACCGc                  >  4:302540/1‑135 (MQ=255)

                                                                                                                              cccccTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCATACGAATCTGTGCGGTATTTCACACCGCatat              >  6:415072/1‑139 (MQ=255)

                                                                                                                               ccccTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCatat              <  2:58177/138‑1 (MQ=255)

                                                                                                                                 ccTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCatat              >  4:82820/1‑136 (MQ=255)

                                                                                                                                 ccTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGt           >  5:23919/1‑139 (MQ=255)

                                                                                                                                   tttCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTg          >  7:278023/1‑138 (MQ=255)

                                                                                                                                    ttCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGa                                                              >  4:194197/1‑85 (MQ=255)

                                                                                                                                    ttCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCa                 >  4:367235/1‑130 (MQ=255)

                                                                                                                                     tCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCTCCTGATGCTGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATgg            <  4:404191/134‑1 (MQ=255)

                                                                                                                                     tCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac                       <  5:398944/123‑1 (MQ=255)

                                                                                                                                     tCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGc         <  5:372447/137‑1 (MQ=255)

                                                                                                                                       gCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCGTGAATGGCGAATGTCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCAct      <  5:343313/138‑1 (MQ=255)

                                                                                                                                       gCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCActc     <  6:360350/139‑1 (MQ=255)

                                                                                                                                       gCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCActc     <  5:43200/139‑1 (MQ=255)

                                                                                                                                        ccAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCActct    <  2:78892/139‑1 (MQ=255)

                                                                                                                                        ccAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCActct    <  7:25497/139‑1 (MQ=255)

                                                                                                                                        ccAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCACCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTATCCTTACGCATCTGTGCGGTATTTCCCACCGCATATGGTGCActct    >  8:167595/1‑139 (MQ=255)

TTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCA  >  Exported/5909‑6185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`