BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,277	A→C	5.6%	intergenic (+66/–)	lacZalpha → / –	/vntifkey=4/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,277	0	A	C	5.6%	20134.8 / 189.9	7357	intergenic (+66/–)	lacZalpha/–	/vntifkey=4/–
Reads supporting (aligned to +/- strand): ref base A (3236/3558); new base C (542/2); total (3786/3571)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 7442 total aligned reads displayed.

CCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGT  >  Exported/6139‑6414

 cTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCAt                                                                                                                                          >  5:134671/1‑139 (MQ=255)

    aCGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATCGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATc                                                                                                                                         <  8:244006/137‑1 (MQ=255)

    aCGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATc                                                                                                                                         <  5:422488/137‑1 (MQ=255)

         tCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTa                                                                                                                                   >  5:163583/1‑138 (MQ=255)

             tgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                                               <  1:311722/138‑1 (MQ=255)

             tgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGAc                                                                                                                              <  7:230936/139‑1 (MQ=255)

                ggTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACa                                                                                                                             <  2:314510/137‑1 (MQ=255)

                  tATTTCGCACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGCCACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCt                                                                                                                         >  7:399169/1‑139 (MQ=255)

                  tATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCACCACCCGCTGACGCGCCCGGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCt                                                                                                                         >  7:372831/1‑139 (MQ=255)

                    tttCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACCCCCGCCAACACCCGCTGACGCGCCCTGCCGGGCGTGTCTGCTCCCCGCCTCCGCTTACAGACAAGCtgt                                                                                                                       >  1:131130/1‑139 (MQ=255)

                       cacaCCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCACCACCCGCTGACGCGCCCGGCCGGGCTTGTCTGCTCCCGGCCTCCCCTTACCGACAAGCGGTGAc                                                                                                                    >  7:175472/1‑139 (MQ=255)

                        acacCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCtgt                                                                                                                       <  4:291453/135‑1 (MQ=255)

                            cGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTa                                                                                                                                   <  8:44920/119‑1 (MQ=255)

                            cGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGtct                                                                                                               >  7:6731/1‑139 (MQ=255)

                              cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCt                                                                                                                                     >  2:385705/1‑115 (MQ=255)

                              cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTcc                                                                                                             <  8:315552/139‑1 (MQ=255)

                              cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAAAACCCGCTGACGCGCCCTGACGGGGGTGTATGCTCCCGCCATACGCATAAAGACAAGAGTTGACCGtc                                                                                                                >  3:235570/1‑136 (MQ=255)

                                  tcgTGCACTCTCAGTACAATCTGCTCTGATGCCTCATAGTTAAGCCAGCCCCGACAGCCGACAACACCCGCTGACGCGCCCTGTCGGGCTTGTGTGCTCCCGGCATCCGCTTACAGACAAGCGGTGACCGTCTCCg                                                                                                            <  3:268227/134‑1 (MQ=255)

                                    gTGCACTCTCAGTCCAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAg                                                                                                        <  7:423278/138‑1 (MQ=255)

                                    gTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCTACACGCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTAAAGACAAGCTGTGAGCGTCTCCGGGAg                                                                                                        <  2:159714/138‑1 (MQ=255)

                                    gTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGTCGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAg                                                                                                        <  5:337672/138‑1 (MQ=255)

                                    gTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAg                                                                                                        <  1:162524/138‑1 (MQ=255)

                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCCTCCGCTTACAGACAAGCTGTGCCCGTCTCCGGGAGCTg                                                                                                     >  1:93227/1‑139 (MQ=255)

                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                                               >  7:197792/1‑113 (MQ=255)

                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCAGACGGGCTTGTATTCTCCCCCCCTCCCCATACAGACAAGCAGTGACCCTCAACGGGaccag                                                                                                     >  2:258941/1‑135 (MQ=255)

                                        agtctcAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCa                                                                                                   <  5:60253/137‑1 (MQ=255)

                                        aCTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCt                                                                                                      <  3:170323/136‑1 (MQ=255)

                                         ctctCAGTACAATCTTCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACa                                                                                                                                 <  4:260909/108‑1 (MQ=255)

                                         ctctCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCAt                                                                                                  >  5:313623/1‑139 (MQ=255)

                                         ctctCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGACGGGCGTGTCTGCTACCCGCATCCGCTTACACACAAGCGGTGACCGTCTCCGGgcgcgggct                                                                                                  >  1:374644/1‑134 (MQ=255)

                                           ctcAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGCCACCCGCCAACACCCGCTGACGCGCCCTGACCGGCTTGTCTGCTACCGGCAACCCCATACAGACCAACGCTGACCCTGGCCGGGCGCTGCa                                                                                                   >  3:140469/1‑136 (MQ=255)

                                           ctcAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCGGTGACCGTCTCCGGGAGCTGCa                                                                                                   >  3:299135/1‑136 (MQ=255)

                                              aGTACAATCTGCTCTGATGCCGCATAGTTAAGCCCGCCCCGCCACCCGCCAACACCCGCTGACGCGCCCTGACCCGCTTGTCTTCTCCCGGCATCCCCTTACAGACAAGCGGTGACCGTCTCCGGGAGCTGGATgtgt                                                                                              >  1:232522/1‑138 (MQ=255)

                                               gTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGTGAGCTGCATGTGTCa                                                                                            <  8:7105/139‑1 (MQ=255)

                                               gTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGACGGGCTTGTCTGCTCCCGGCCTCCGCGTAACGACAAGCTGTGCCCGTCTCCGGGAGCTGCCTgtgt                                                                                              >  6:79590/1‑137 (MQ=255)

                                                    aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTTCTCCCGGCCTCCGCTTAAAGACAAGCTGTGCCCGTCGCCGGGCGCTGCATGTGTCAGAGGt                                                                                       >  8:28238/1‑139 (MQ=255)

                                                    aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                                               >  6:349851/1‑99 (MQ=255)

                                                    aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCa                                                                                            >  6:160723/1‑134 (MQ=255)

                                                    aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCGGTGACCGTCTCCGGGAGCTGCATGTGGCAGAGGt                                                                                       >  1:344822/1‑139 (MQ=255)

                                                    aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCCCTAACAGACAAGCTGTGACCGTCTCCGGGAGCGGGATGTGTCAGAggg                                                                                       >  2:346479/1‑138 (MQ=255)

                                                    aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCACCCCGCAACCGCTTACAGACAAGCGGTGACCGTCGCCGGGAGCTGCATGTGTCAGAGGt                                                                                       >  5:290608/1‑139 (MQ=255)

                                                    aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCATGTCTGCTCCCCGCCTCCGCATAAAGACAAGCAGGTACCGGCTCCGGGAGCTGCATGGTTAAGAGGt                                                                                       >  8:1501/1‑139 (MQ=255)

                                                     tCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGtt                                                                                      >  6:379151/1‑139 (MQ=255)

                                                        gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCCTCCGCTTACAGACCAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGtttt                                                                                    >  6:128876/1‑138 (MQ=255)

                                                        gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGCGGtttt                                                                                    >  2:12552/1‑138 (MQ=255)

                                                        gCTCTGATGCCGCATAGATAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGtttt                                                                                    >  6:410248/1‑138 (MQ=255)

                                                         ctctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACGCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCa                                                                                  <  7:142257/139‑1 (MQ=255)

                                                          tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACACACAAACAGTGACCGTCTCCCGGACCTGCATGTGTCAGAggctgtct                                                                                  >  8:182698/1‑132 (MQ=255)

                                                          tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCAGACGGGCGTGTCTGCACCCCGCATCCCCATTCACACAAACTGTGACCCTCTCCCGGGGCTGCATGTGTCAGAGGTTTTAAc                                                                                 >  5:367584/1‑139 (MQ=255)

                                                          tctGATGCCGCATAGTTAAGCCACCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGCGCGTGTAATCACCCGGCATACGCATTAATAGAAGCTGTTACCGACTGCGGGAGCTTCAATTTTAAGAGGgttttcc                                                                                 >  6:31918/1‑136 (MQ=255)

                                                           ctGATTCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCAGACGGGCTAGTCTGCTCCCCGCAACCCCTTACACACAATCTGTGACCCTCTCCCGGAGCTCCATGAGTCAGAGGattt                                                                                    >  4:132309/1‑135 (MQ=255)

                                                           ctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACCCCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTTCAGCCAAGCGGTGCCCGTCGCCGGGCGCTGCATGGGTCAGCGGGTTTc                                                                                   >  4:190515/1‑136 (MQ=255)

                                                             gATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCTGGGAGCTGCATGTGTCAGAGGTTTTCAc                                                                                 >  3:110890/1‑136 (MQ=255)

                                                              aTGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCTCCCGGCATCAGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGACGTTTTAACCGt                                                                              >  8:344695/1‑138 (MQ=255)

                                                              aTGCCCCATAGTTAAGCCACCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGCGCATGTCTTCTCCCGGCCACCGCTTACAGACAACCAGTGACCCTCACCCGGAGCGGCATCTGTCACAGGATTTCACCGt                                                                              >  1:28595/1‑138 (MQ=255)

                                                                      tAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCCTCAc                                                                        >  3:389587/1‑136 (MQ=255)

                                                                           aaGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAAcgc                                                                >  6:215651/1‑139 (MQ=255)

                                                                            gacaggccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAAcgcg                                                               <  5:394769/134‑1 (MQ=255)

                                                                            agccagccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTa                                                                                                                                   <  5:214699/71‑1 (MQ=255)

                                                                               cagccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCga                                                            <  5:249022/139‑1 (MQ=255)

                                                                                 gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGCAACGCGCgaga                                                          >  7:94853/1‑139 (MQ=255)

                                                                                 gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCgaga                                                          >  1:2006/1‑139 (MQ=255)

                                                                                  ccccGACACCCGCCAACACCCGCTGACGCGCCCGGACGGGCTTGTCTGCTCCCCGCATCCGCTTACAGCCAAGCCGTGACCGTCTCCGGGAGGGGCAAGTGTCAGAGGTTTTCAACGTCATCACCGAAACGCGCGAGAc                                                         >  7:297164/1‑139 (MQ=255)

                                                                                   cccGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCCCTTACAGACAAGCAGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGATAc                                                         >  7:112777/1‑138 (MQ=255)

                                                                                    ccGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCgaga                                                          >  3:41795/1‑136 (MQ=255)

                                                                                    ccGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCCGCCTCCCCGTAAACACAAACAGTGCCCGACTCCGGGAGCAGCATTTGTCAGAGGGTTTCACCGTCCTCACCCAAACGCGCgaga                                                          >  4:77358/1‑136 (MQ=255)

                                                                                    ccGACACCCGCCAACACCCGCTGACGCGCCCAGACGGGCGTGTCTTCACCCGGCCTACGCAAACAAACAAACAGTGAACGGCACCCCGAGCTGCCAGTGTGAAAGGTTTTGACCGTCAACACCGCAACGCGCgagc                                                          >  4:428032/1‑135 (MQ=255)

                                                                                     cGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCGTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCCGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGaa                                                      <  1:100535/139‑1 (MQ=255)

                                                                                      gACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACg                                                        <  4:396724/136‑1 (MQ=255)

                                                                                         acCCGCCAACACGCGCTGCCGCTCACTGACGGGCTTGTCTGCTCCCGGGTTGCGCTTACAGAGAAGCTGTGACCGTTTCCTGTAGCTGGATGTGTGAGAGGTTTTCTCCGTCATCACAGAAAGGGGCGTGACCATAggg                                                  <  2:7901/139‑1 (MQ=255)

                                                                                           ccGCCAACACCCGCTGACGCGCCCGGCCGGGCGTGTCTGCTCCCGGCCTCCGCTTTAAGCCCAGCGGTGACCGTCGCCGGGGGCGGCCTGTGTGAGAGGTTTTAACCGTCCTACCCGACACGCGCGGGCCGCAAggggc                                                >  2:233249/1‑137 (MQ=255)

                                                                                            cGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCt                                               <  8:122656/139‑1 (MQ=255)

                                                                                              ccAACACCCGCTGACGCGCCCTGACGGGCCTGTCAGCTCCCGGCAACCCCTTACAGACCAGCAGTGACCGGCGCCCGGCGCGGCATGTGTCAGAGGTTTTCACCGTCATCACCGCAACGCGCGAGCCGAAAGGGcc                                                >  3:62322/1‑136 (MQ=255)

                                                                                                 acacCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGa                                          <  7:261099/139‑1 (MQ=255)

                                                                                                     ccGCGGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACg                                      <  6:18875/139‑1 (MQ=255)

                                                                                                      cGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCggg                                                                                                          >  7:351413/1‑70 (MQ=255)

                                                                                                       gCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCt                                               <  5:107394/128‑1 (MQ=255)

                                                                                                       gCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATAc                                       <  3:359946/136‑1 (MQ=255)

                                                                                                        cTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACg                                      <  4:357816/136‑1 (MQ=255)

                                                                                                        cTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCt                                   <  6:296153/139‑1 (MQ=255)

                                                                                                              cgcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTAttttt                             <  6:430752/139‑1 (MQ=255)

                                                                                                               gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                            <  1:257409/139‑1 (MQ=255)

                                                                                                                 ccTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTtata                          <  5:185388/139‑1 (MQ=255)

                                                                                                                        ggCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAAt                   <  1:58094/139‑1 (MQ=255)

                                                                                                                          cTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGt                 >  2:19978/1‑139 (MQ=255)

                                                                                                                             gTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGt                                            >  4:323009/1‑109 (MQ=255)

                                                                                                                             gTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTa                                  <  6:252391/119‑1 (MQ=255)

                                                                                                                             gTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGt                 >  3:9808/1‑136 (MQ=255)

                                                                                                                             gTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCa               <  8:402084/138‑1 (MQ=255)

                                                                                                                             gTCTGCTCCCGGCATCCGCTTACAGACAAGCTGAGACCGTCTACGGGAGCTGCAAGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGAGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGTTTAATg                  >  4:157876/1‑135 (MQ=255)

                                                                                                                              tCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGt                 <  3:201911/135‑1 (MQ=255)

                                                                                                                              tCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCAt              <  2:48478/138‑1 (MQ=255)

                                                                                                                              tCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCAt              <  2:286661/138‑1 (MQ=255)

                                                                                                                              tCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGAGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGt                 <  4:226285/135‑1 (MQ=255)

                                                                                                                               cTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGa            >  1:417129/1‑139 (MQ=255)

                                                                                                                               cTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGa            <  5:9458/139‑1 (MQ=255)

                                                                                                                                     ccGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGagaa         >  3:324655/1‑136 (MQ=255)

                                                                                                                                       ggCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCAt              <  8:372114/129‑1 (MQ=255)

                                                                                                                                       ggCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATg    >  8:206276/1‑139 (MQ=255)

                                                                                                                                       ggCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATg    >  7:324640/1‑139 (MQ=255)

CCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGT  >  Exported/6139‑6414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`