| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 1,474,968 | G→T | 35.1% | S88* (TCA→TAA) | U712_07575 ← | Uncharacterized protein ykoA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 1,474,968 | 0 | G | T | 35.1% | 45.1 / 15.1 | 44 | S88* (TCA→TAA) | U712_07575 | Uncharacterized protein ykoA |
| Reads supporting (aligned to +/- strand): ref base G (4/24); new base T (13/2); total (18/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.22e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGAAACGGGCTGGGACTGATCGCGGAAGATCGAATTGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACCAATGCAATGCATAAAACCGTGATG‑AACACTGAACCGATAATAGCCAGCACACTGAAGAAAAGGTGAACCTGTTTGTTCTCTTTTCCGCGCTTTTCTAATAGCCGTTTAGAAA > CP006881/1474834‑1475094 | ccGAAACGGGCTGGGACTGATCGCGGAAGATCGAATTGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑GTTTATTTTTaaa > 1:236378/1‑138 (MQ=255) acggggCGGGTACTTATCGCGTAATATCTAATTGGGGGAACATCTAATT‑TTGTTTTTTACCCTTTAAAC‑CAAATGCTACAAAAAAAAAAAAACCGCCTTGCTGGCCTAGGACCGCAGGCGT‑TTTTATTTTGaaaaa < 4:294254/133‑1 (MQ=255) aaggggCTGGTACTGATCGCGCAATATCGAATTGTCGGAACATCTATGT‑TTGTCTTTTTCCCGTTAAAC‑TAAATGCGTCAAAAAAAAAAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGaaaaa < 3:276758/133‑1 (MQ=255) gcggggACTGATCGCGGACGATCGAATTGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGTTAAAC‑GAAATGCTTAAAAAAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGAc < 5:374344/136‑1 (MQ=255) cTGGGACTGATCGCGGAAGATCGAATTGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTTATAAAGAAGCGAc > 1:115959/1‑138 (MQ=255) cTGGGACTGATCGCGGAAGATCGAATTGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGGTTAAC‑GAAATGCCTCAAACAAAAAAAAAACGCCTTGCTGGCCTAGGACACCAGGCGTGGTTTTTTTTAAAAAAAAAGCAAc > 5:34100/1‑139 (MQ=255) ggggACTTACCGCGCAACATCTAATGGGCGGAACAGCGATTT‑TTGACTTTTTCCCGTTAAAC‑AAAATGCGTAAAAAAAAAAAAACACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACca < 1:80610/138‑1 (MQ=255) gACTGATCGCGGAAGATCGAATTGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACcaatg < 7:59371/139‑1 (MQ=255) ctcaTCGCGTAACTTCGAATTGTCGCCACATCTAAGT‑TTGTGTTTTCCCCGTTTAAC‑TAAATCCCTAAAAAAAAAAAAAACCGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACcaatgca < 5:420168/136‑1 (MQ=255) cTGATCGGGGACGATCTAATTGTCGGCACATCGAAGT‑TTGACTTTTTCCCGTTTAAC‑GAATTGGGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACcaatgca < 7:330707/139‑1 (MQ=255) cTGATCGCGGAAGATCGAATTGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACcaat < 3:169009/136‑1 (MQ=255) cTGATCGCGGAAGATCGAATTGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAAAAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACcaatgca < 7:331806/139‑1 (MQ=255) cTGAGCGGGGAAGTTGTAATTGTGGGCACATCGATGT‑TTTTCTTTTTCCCTTTTAAC‑TAAATGCGTAAAAAAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACcaat < 3:53991/136‑1 (MQ=255) cTGAGCGCGGACGATCTAATTGTCGGCACATGGAATT‑TGGTGTTTTTCCCGTTAAAC‑TAATTGCGTCAAAAAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACcaatgca < 2:276327/139‑1 (MQ=255) aTCGCGGAAGATCGAATTGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTTTTTTTAAAAAGAAGCGTCcaaaggaat > 5:313164/1‑138 (MQ=255) aTCGCGGAAGATCGAATTGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACcaatgcaatg > 7:62174/1‑139 (MQ=255) aTCGCGGAAGATCGAATTGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑GTTTTTTTTTTAAAAGAACGGACcaatggaatt > 7:163325/1‑138 (MQ=255) gcgGAAGATCGAATTGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑GTTTTTTTTTAAAAAAAAGCGACcaattcaatg > 3:259262/1‑136 (MQ=255) gttaGATCTAATTGACGGCACATCGATTT‑TTTTCTTTTTCCCGTTTAAC‑TAAATGCGTCAAAAAAATTAAAAACGCCTTGGTGGCCTTGGACAGCAGGGGT‑TTTTATTTTGAAAAAGAAGCGACCAatgcaatgca < 4:200008/133‑1 (MQ=255) ggAAGATCGAATTGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTTTTTTTAAAAACAAGCCGCCCatgcaaagcaaa > 2:134010/1‑136 (MQ=255) gATCTAATTCTCGCCACATCGATGT‑TTGTATTTTTCCCTTTAAAC‑TAAAGCCGTAAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACCAATGCAATGCATAAAAcc < 5:12153/139‑1 (MQ=255) aTCGAATTGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGCCAGCAGGCGT‑TTTTATTTTTAAAAAGCAGCGACCAAAGCAAAGCATAAAACCg > 8:137826/1‑139 (MQ=255) aTCGAATTGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTTAAAAAGAAGCGACCAAAGGAATTCATAAAAccc > 2:267370/1‑138 (MQ=255) cGAAGTGGCGGAACCTCGACGT‑TTGTCTTTTTCCCGTTAAAC‑GAATTGCGTCAAACAAAAAAAACACGCCTTGCTGGCCTAGGCCAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACCAATGCAATGCATAAAACCGtg < 2:349814/139‑1 (MQ=255) taTTGTCGGCAAATCGAAGT‑TTGTCTTTTACCCGTTAAAC‑CAAATGCGTAAAACAAAAAAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACCAATGCAATGCATAAAACCGtgat < 8:86938/138‑1 (MQ=255) atttGTCGGAACATCGAAGT‑TTTTCTTTTACCCGTTAAAC‑TAAATGCGTCAAAAAAAAAAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTTAAAAAGAAGCGACCAATGCAATGCATAAAACCGtgat < 1:267370/137‑1 (MQ=255) tttGTGGGCACATCTAAGT‑TTGTATTTTTGCCGTTTAAC‑TAATTGCTAAAAAAAAAATAAAAACGGCTTGGTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACCAATGCAATGCATAAAACCGtgat < 6:313164/137‑1 (MQ=255) aTTTTCGGAACATCGTAGTGTTGT‑TTTTTCCC‑TTTAACAGAAAGGCTAAAAAAAAAAAAAAAACGCCTTGCTGGCCTAGGAAAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACCAATGCAATGCATAAAACCGtgat < 5:139140/138‑1 (MQ=255) aTTGTCGGCAAATCCTATT‑TTTTTTTTTTCCCGTTTAAC‑TAAATGCGTCAAAAAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACCAATGCAATGCATAAAACCGtgat < 6:341689/138‑1 (MQ=255) tGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTTTTTTTTAAAAAAAGAGGCCAAATCAAATCATAAAAACCtgatta > 2:346399/1‑136 (MQ=255) tCGCCACAGCTATGT‑TTGTTTTTTTCCCTTTTAAC‑TAAATGCTTCAAAAAAATAAAAAACGCCTTGCTGGCCTAGGACAGCTGGCGT‑TTTTATTTTGAAAAAGAAGCGACCAATGCAATGCATAAAACCGTGATG‑Aaca < 5:47213/139‑1 (MQ=255) ggCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTTTAAAAGAAGCGACCAATGCAATGCATAAAACCGTGATT‑AACACt > 7:3721/1‑139 (MQ=255) ggCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTTAAAAACAAGCGACCAATGCAATGCATAAAAACCTGATG‑aaaa > 7:9839/1‑135 (MQ=255) caTCGATTT‑TTTTCTTTTTCCCTTTAAAC‑TAAATGCGAAAAAAAAAAAAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACCAATGCAATGCATAAAACCGTGATG‑AACACTGAAc < 1:346399/139‑1 (MQ=255) aaGT‑TTGTCTTTTTCCCGATAAAC‑CAAATGCGTCAAACAAAAAAAACACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACCAATGCAATGCATAAAACCGTGATG‑AACACTGAACCg < 3:82308/136‑1 (MQ=255) gTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACCAATGCAATGCATAAAACCGTGATG‑AACACTGAACCGataat < 3:212129/135‑1 (MQ=255) tCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTTTTTTTTAAAAAAAACACCCCATTCAATTCATAAAAACAGAATGAAACAC‑GAACCGACAATAACCa > 2:402755/1‑139 (MQ=255) accGTTAAAC‑AAATTGCGAAAAAAAAATTTAAACCTCATTGCTGGCCTAGTCATGCTGGCGT‑TTTTATTTTTAATAAGAAGCGACCAATGCAATTCATAAAACCGTGATG‑AACACTGAACCGATAATAGCCAGCacac < 5:18248/137‑1 (MQ=255) cGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑GTTTATTTTGAAAAAGAAGCGACCAATGCAATTCATAAAACCCTGATT‑AACACAGAACCGATAATAACCAGcaca > 3:322570/1‑135 (MQ=255) taatcaaaTCCGTTAAAAAAATTAAAATGGCTTTGCTGGCCTAGGAGAGCTGGCGT‑TTTTATTTTGAAAAAGAAGCGTCCTATGCAATGCATTAAACCGTGATG‑AACACTGAACCGATAATAGCCAGCACACTga < 3:110755/130‑1 (MQ=255) tGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑GTTTTTTTTGTAAAAGAAGCGCCCAATGCAATGCATAAAACCGGGATG‑AACACTGAACCGATAATAGCCCGCCCACTGAAGAAAAGGTgga > 5:337522/1‑137 (MQ=255) cGCCTTGCTGGCCTAGGACAGCAGGCGT‑GTTTTTTTTGTAAAAGAAGCGACCAATGCAATGCATAAAACCGTGATG‑AACACTGAACCGATAATAGCCAGCACACTGAAGAAAAGGTGAACCTGTTTGTTCTCtttt > 3:198486/1‑136 (MQ=255) cTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACCAATGCAATGCATAAAACCGTGATG‑AACACTGAACCGATAATAGCCAGCACACTGAAGAAAAGGTGAACCTGTTTGTTCTCTTTTCCGCGCttt < 6:337522/138‑1 (MQ=255) ttttttttTAAAAATAAGCGACCAATGCAATGCATAAAACCGGGATG‑GACAAAGAACCGATAAAAACCAGCACACTGAAGAAAAGGTTAACCGGTTTGGTGTCTTTTAAGCGCTTTTATAATAGCCGTTTAGaaa > 4:106217/1‑135 (MQ=255) | CCGAAACGGGCTGGGACTGATCGCGGAAGATCGAATTGTCGGCACATCGAAGT‑TTGTCTTTTTCCCGTTTAAC‑GAAATGCGTCAAACAAAATAAAAACGCCTTGCTGGCCTAGGACAGCAGGCGT‑TTTTATTTTGAAAAAGAAGCGACCAATGCAATGCATAAAACCGTGATG‑AACACTGAACCGATAATAGCCAGCACACTGAAGAAAAGGTGAACCTGTTTGTTCTCTTTTCCGCGCTTTTCTAATAGCCGTTTAGAAA > CP006881/1474834‑1475094 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |