| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 1,831,004 | C→T | 6.1% | G59G (GGC→GGT) | U712_09105 → | Uncharacterized protein ymzC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 1,831,004 | 0 | C | T | 6.1% | 207.3 / 3.2 | 66 | G59G (GGC→GGT) | U712_09105 | Uncharacterized protein ymzC |
| Reads supporting (aligned to +/- strand): ref base C (35/26); new base T (2/2); total (38/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01 | |||||||||||
CACTTGTATGGATAGCTGTCTTTTTACTGTTCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCATTCGAATTTACAAAGCGGATGCCAGCCGCT > CP006881/1830868‑1831142 | cACTTGTATGGATAGCTGTCTTTTTACTGTTCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGt > 8:87925/1‑139 (MQ=255)cACTTGTATGGATAGCTGTCTTTTTACTGTTCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGt > 2:380848/1‑139 (MQ=255) gTATGGATAGCTGTCTTTTTACTGTTCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGACCATAACCATTTTTGCGTTATgg > 6:285732/1‑139 (MQ=255) gTATGGATAGCTGTCTTTTTACTGTTCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATg < 6:141930/138‑1 (MQ=255) gTATGGATAGCTGTCTTTTTACTGTTCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATg < 2:32200/138‑1 (MQ=255) gATAGCTGTCTTTTTACTGTTCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGAc > 5:224533/1‑139 (MQ=255) aTAGCTGTCATTTTACTGTTCGGGGCGTGCGGGAATCAAGAAACCATTATTGAAACCGACAACGCCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACGTGAACATAACCATTTTGGCGTTATGGAGGACg > 8:89042/1‑139 (MQ=255) aGCTGTCTTTTTACTGTTCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGAc > 3:284160/1‑136 (MQ=255) cTGTCTTTTTACTGTTCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGTGTTATggag > 7:34461/1‑131 (MQ=255) cTGTCTTTTTACTGTTCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGTGTTATggag < 8:34461/131‑1 (MQ=255) cTGTCTTTTTACTGTTCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCta > 6:115829/1‑139 (MQ=255) cTGTCTTTTTACTGTTCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAAAGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCta > 2:104115/1‑139 (MQ=255) tttttACTGTTCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATc > 1:25456/1‑138 (MQ=255) attCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTa < 8:93660/137‑1 (MQ=255) attCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCAACCTCGTTTCCACTTGAACATAACCATTTTTGCGTTATGGAGGACGGCTATATCAAAATTTa > 7:93660/2‑138 (MQ=255) ttCGGGGCGTGCGGGAATCAAGATACCATTATTGAAATAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTa < 5:7759/137‑1 (MQ=255) ttCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAAtt < 4:342354/135‑1 (MQ=255) ttCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAAtt < 4:258275/135‑1 (MQ=255) ttCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTa < 8:138527/137‑1 (MQ=255) ttCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTa < 1:95550/137‑1 (MQ=255) ttCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTAt < 6:117505/138‑1 (MQ=255) tCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGa > 6:303026/1‑139 (MQ=255) gTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGtat < 4:284160/136‑1 (MQ=255) gCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACgg < 7:326865/112‑1 (MQ=255) gCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACgg > 8:326865/1‑112 (MQ=255) gCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAAt > 6:395980/1‑137 (MQ=255) aataTACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCg < 3:369250/133‑1 (MQ=255) aaGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCaa < 1:348725/139‑1 (MQ=255) aaGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCaa < 2:67414/139‑1 (MQ=255) aaGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCaa < 1:380848/139‑1 (MQ=255) aCCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCaa < 6:33722/134‑1 (MQ=255) aCCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCaa > 5:33722/1‑134 (MQ=255) cATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAg < 3:150213/136‑1 (MQ=255) ttGAAACAGACAACGGCAATTCAGACTATGAAATGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGc < 2:30293/138‑1 (MQ=255) aCAGACAACGGCAATTCAGACTATGAACCGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTgaag > 2:391242/1‑138 (MQ=255) gACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGTGTTATGGAGGACGGCTATATCAAAATTTAt < 8:380476/99‑1 (MQ=255) gACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGTGTTATGGAGGACGGCTATATCAAAATTTAt > 7:380476/1‑99 (MQ=255) aCAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGaagaaag > 1:320906/1‑138 (MQ=255) ttCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGgat > 1:305099/1‑139 (MQ=255) aCTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGgatgat > 7:80544/1‑137 (MQ=255) aTGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGgatgat > 3:354483/1‑134 (MQ=255) cGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGTCGTTATGGAGGCCGGATATATAAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGATGAATAAAGAATACGCGGATGATGAGCTTGAATa < 2:21067/139‑1 (MQ=255) cTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATaaaa > 2:316957/1‑139 (MQ=255) tCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAAtt > 3:246483/1‑67 (MQ=255) tCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAAtt < 4:246483/67‑1 (MQ=255) ccACCTCGTTTCCACTTTAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTcaca < 5:285732/139‑1 (MQ=255) ccTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAg > 2:27584/1‑138 (MQ=255) ccTCGTTTCAACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGAGGATGATGAGCTTGAATAAAATTCACAAg > 6:174532/1‑138 (MQ=255) cTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGTGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCa > 2:278105/1‑139 (MQ=255) cTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTAt < 1:163167/61‑1 (MQ=255) cTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTAt < 1:125386/61‑1 (MQ=255) cTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTAt > 2:125386/1‑61 (MQ=255) cTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTAt > 2:163167/1‑61 (MQ=255) cTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCa > 7:305416/1‑139 (MQ=255) gTTTCCACTTGAACGTAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCAtt < 7:98874/138‑1 (MQ=255) ttCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCATTCg > 7:16334/1‑138 (MQ=255) cTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGCGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCATTCGAATTTa > 7:291337/1‑139 (MQ=255) gAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCATTCGAATTTACa < 2:320906/138‑1 (MQ=255) gAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCATTCGAATTTACa > 1:385045/1‑138 (MQ=255) cATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCATTCGAATTTACa > 4:402156/1‑135 (MQ=255) aaCCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCATTCGAATTTACAAAGCGGa > 8:102176/1‑139 (MQ=255) aaCCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCATTCGAATTTACAAAGCGGa > 6:377963/1‑139 (MQ=255) aTTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCATTCGAATTTACAAAGCGGATGcc < 2:305099/139‑1 (MQ=255) ttttGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCATTCGAATTTACAAAGCGGATg > 4:217505/1‑136 (MQ=255) ttttGGAGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCATTCTAATTTACAAAGCGGATGCCa > 7:74566/1‑139 (MQ=255) tttGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCATTCGAATTTACAAAGCGGATGCCAg > 8:390577/1‑139 (MQ=255) cGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCATTCGAATTTACAAAGCGGATGCCAGCCGct < 7:87925/139‑1 (MQ=255) | CACTTGTATGGATAGCTGTCTTTTTACTGTTCGGGGCGTGCGGGAATCAAGATACCATTATTGAAACAGACAACGGCAATTCAGACTATGAAACGCCTCAGCCCACCTCGTTTCCACTTGAACATAACCATTTTGGCGTTATGGAGGACGGCTATATCAAAATTTATGAGTATAATGAGTCCCGCAATGAGGTAAAGCTGAAGAAAGAATACGCGGATGATGAGCTTGAATAAAATTCACAAGCATTCGAATTTACAAAGCGGATGCCAGCCGCT > CP006881/1830868‑1831142 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |