| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 2,071,605 | G→T | 23.4% | intergenic (‑232/‑43) | U712_10240 ← / → U712_10245 | Uncharacterized protein yojJ/putative multidrug resistance protein norM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,071,605 | 0 | G | T | 23.4% | 119.7 / 3.0 | 63 | intergenic (‑232/‑43) | U712_10240/U712_10245 | Uncharacterized protein yojJ/putative multidrug resistance protein norM |
| Reads supporting (aligned to +/- strand): ref base G (29/17); new base T (2/13); total (33/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.73e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGAAAGAATCATTCAGTATATTTGCACCTTTTTAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑TATGAAAGAAACTCAAAACGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTAATACCGATATTCATTACCCAAGCGGGGCTTTCTCTGATCA > CP006881/2071469‑2071741 | tCGAAAGAATCATTCAGTATATTTGCACCTTTTTAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGtt < 8:61097/139‑1 (MQ=255) gAAAGAATCATTCAGTATATTTGCACCTTTTTAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTt‑at < 7:418326/139‑1 (MQ=255) gAAAGAATCATTCAGTATATTTGCACCTTTTTAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTt‑at < 8:327825/139‑1 (MQ=255) gAAAGAATCATTCAGTATATTTGCACCTTTTTAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTt‑a > 7:110006/1‑138 (MQ=255) ttGCACCTTTTTAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTTCTTTTTATACATAAAATGGT‑ATAATTTCTCTAGTTATTaaa > 7:203074/1‑138 (MQ=255) ttGCACCTTTTTAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATTTT‑ATAATTTCTCTCGTTATGaaa > 7:146589/1‑138 (MQ=255) ttGCACCTTTTTAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGaaa > 1:282724/1‑138 (MQ=255) tGCACCTTTTTAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGaaaa < 2:76700/138‑1 (MQ=255) gCACCTTTTTAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAAAGTT‑ATAATTTCTCTCGTTATGaa > 3:78347/1‑135 (MQ=255) cACCTTTTTAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGaaa > 4:134517/1‑135 (MQ=255) ccTTTTTAGTCTTGATTTCTCTTTTTTTTATTTATGCCAAAATGTTTATAAAAACTAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATTAAAAAg < 3:324841/136‑1 (MQ=255) ccTTTTTAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAATTGTTTATAAAAACTAAACCGACAAAAAATGATTAGGCCCGGCGTTCATTGGTTTTTATAAATAAAATGTT‑ATAATTTAGATCGTTATGAaaaaaaaa > 5:110574/1‑139 (MQ=255) ccTTTTTAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGaaa > 5:261413/1‑139 (MQ=255) ccTTTTTAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGaaa > 6:159195/1‑139 (MQ=255) ccTTTTTAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGaaa > 7:82031/1‑139 (MQ=255) tttttAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATTAAAAAGAAACg > 5:312729/1‑139 (MQ=255) tttttAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTAGTTATGAAAAAGAAACg > 1:343836/1‑139 (MQ=255) ttttAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTTCTTTTTATACATAAAATGTT‑ATAATTTCTCTAGTTATGAAAAAGaa > 4:324841/1‑135 (MQ=255) ttttAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATAAATAAAATGTT‑ATTATTTAGATAGTTATTAAAAAGAAACgg > 1:158562/1‑139 (MQ=255) ttttAGGCGTGATTTCGCTTTCTTTTATTTATGACCAAATGTTTAGAAAAACGAACCCGACAAAAAAAGAATAGGCCCGGCGTTGATTGCTTTTTTTACATAAAATTTT‑ATAATTTGTGTGGTTATGATAAAGAAACgg > 1:391717/1‑139 (MQ=255) tttAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACg > 6:140390/1‑137 (MQ=255) tAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGGTTTTTATACATAAAATGTT‑ATAATTTCTCTAGTTATGATAAAGAAACg > 3:57862/1‑135 (MQ=255) ggggTGATTTCGCTTTCTTTTATTTATGCCAAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGAt < 2:3406/135‑1 (MQ=255) gggggATTTCGCTTTGTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCCACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCa < 6:110574/135‑1 (MQ=255) ctgttttttCGCTTTCTTTTATTTTTCACCAATTTTTAACAAAAACTAAACCTAAAAAAAAAGAATAGGCCCGGCGTTCATTGATTTTTATACATAAATTGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCa < 2:361867/133‑1 (MQ=255) aTTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑Ta > 5:323873/1‑139 (MQ=255) aTTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑Ta > 1:318761/1‑139 (MQ=255) ttctttTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑TATga > 4:394774/1‑134 (MQ=255) ctttTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑TATGAAAGAAAc > 8:219973/1‑139 (MQ=255) ctttTATTTATGCCCAAATGTTTACAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑TATgaaagaaa < 5:350058/138‑1 (MQ=255) tttatttaTGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAATAAACGGAGATAAATG‑TATgaaagaa > 3:277955/1‑135 (MQ=255) gCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑TATGAAAGAAACTCAAAACGccc > 8:389639/1‑139 (MQ=255) ccccAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGGTTTTTTTAATTAAAATATT‑AAAAATTCACTCGTTATGAAAAAGAAACTGAGATAAAAT‑TATTAAAAAAACTCAAAACGccc > 7:163760/2‑139 (MQ=255) cAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑TATGAAAGAAACTCAAAACGccc > 3:155127/1‑136 (MQ=255) gTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑TATGAAAGAAACTCAAAACGCCCGGGa > 4:295907/1‑135 (MQ=255) tttAGAAAAACGAAACCGACAAAAAAGGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑TATGAAAGAAACTCAAAACGCCCGGGaaaaaa > 2:3022/1‑139 (MQ=255) aacacaaaaCCAAAAAAAAAAAAATCGGCCCGGCGTTCATTGTTTTTTAAAAAAAAAATTTT‑ATATTTTCTCTCGTTATCAAAACGAAACGCCGATAAATT‑TATAAAACAAACCAAAAACGCCCGGGAAAAAATCAAAc < 2:343836/133‑1 (MQ=255) aaacccaaaCCCACAAAAAAAGAATCGGCCCGGCGTTCATTGCTTTTAAAAAATAAATTTTT‑ATAATTTCTCTCGTTATCAAAAACAACCGGATATCAATG‑TATCAAACAAACTCAAAACGCCCGGGAAAAAATCAAAc < 7:81520/133‑1 (MQ=255) aaaaCGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑TATGAAAGAAACTCAAAACGCCCGGGAAAAAATCAAAc < 7:219973/139‑1 (MQ=255) aaaaCGAAACCCCCAAAAAACGAAACCGCCCGGCGTTCATTGCTTTTTATAAATAAAATTTT‑ATAATTTCTCTCGTTATCAAAAACAACCGTAGATCAATG‑TAGCAAAGAAACTCAACACGCCCGGGAAAAAATCAAAc < 6:74608/139‑1 (MQ=255) aaaaCCAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑TATGAAAGAAACTCAAAACGCCCGGGAAAAAATCa < 3:182924/136‑1 (MQ=255) aaCCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑TATGAAAGAAACTCAAAACGCCCGGGAAAAAATCAAACAGct > 3:7183/1‑136 (MQ=255) ctccAAAAAATTAATAGCGCCTGCGTTAATTTCTTTTATTACAAAAATTGTT‑ATATTTTCTCAGTTTATGAAAAACAAACGTATAATAATG‑TATAAAATAAACCAAAAACGCCCGGGAAAAAATCAAACAGCTCTTGca < 6:282282/136‑1 (MQ=255) ctaaaaaaaaaCAATCGGCCCGGCGTTCATTGCTTTTTATAAATAAAATTTT‑ATAATTTCTCTCTTTATCAAAAACAAACGGATATCAATG‑TATGAAACAAACTCAAAACGCCCGGGAAAAAATCAAACAGCTCTTGca < 8:82031/135‑1 (MQ=255) cGACAAAAAAGAAATTGGCCCGGCGTTCATTGCTTTTTATAAAAAAATTTTT‑ATATTTTCTCTCGTTATTAAAAACAAACGGAGATCATTT‑TATCAATTAAACTCAAAACGCCCGGGAAAAAATCAAACAGCTCTTGca < 1:3022/139‑1 (MQ=255) ccaaaaaaaaaaaaaCGCCCCGGGTTAATTTTTTTTTAAAAAAAAAATTTTAATAATTTCTCTCTTAAAAAAAAACAAAGGAACATAATTT‑TATCAAACAAACACAACACGCCCGGGAAAAAATCAAACAGCTCtt < 4:7183/133‑1 (MQ=255) aCAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTATCTCGTTATGAAAAAGAAACGGAGATCAATG‑TATGAAAGAAACTCAAAACGCCCGGGAAAAAATCAAACAGCTCTTGcaca > 5:199431/1‑139 (MQ=255) aaacactAGGCCCGGTTTTAATTTTTTTTAATAAAAAAATTTTA‑ATAATTAATTTCTTAATAAAAACAAACCGTATATAATTT‑TATTAAATAAACAAAAAACGCCCGGGAAAAAATCAAACAGCTCTTGCACAttt < 4:245258/130‑1 (MQ=255) aaaTAATAGGCCGGGGGTTCATTGCTTTTTATACATAAAATTTA‑ATTATTTCTCTCTTTATCAAAAACAAACTCATATAAATT‑TATCAAACAAACTAAAAACGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTaa < 8:146589/139‑1 (MQ=255) aaaCAATAGGCCCGGCGTTCTTTGATTTTTAAAAATAAATTTTT‑ATAATTTCTCTCGTAATGAAAACTAAACGGATATCAATG‑TATCAAAGAAACTCAACACGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTaa < 8:11407/139‑1 (MQ=255) caacccgCCGGGCTTTAATTGATTTTAAAAAAAAAAATTTA‑AAAATTTCTCTCTTAATAAAAACAAACCGTACATAAATG‑AATCAAAAAACCACAAAACGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTAATAc < 6:261413/133‑1 (MQ=255) ttggCCCGGTGTTCATTGTTTTATAAACTTAAATTTTT‑AATATTTTTCTCTTTTTTAAAAACAAACGTTGAT‑ATTGTTTTGAATGAAATTCACCACGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTAATAc < 4:78347/134‑1 (MQ=255) ggCCCGGCTTTCATTGCTTTTAATACATAAATTTTT‑ATAATTTCTCTCGTAATCAAAAACAAACGGCCATCAATG‑TATGAAACAAACTCAAAACGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTAATACCGat < 5:159195/138‑1 (MQ=255) ggCCCGGCGTTAATTCCTTTTTATACATAAAATGTT‑AAAATTTCTCTCGTTATCAAAAACAACCGGAGATCAATG‑TATCAAAGAAACTCAACACGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTAATACCGata < 1:314416/139‑1 (MQ=255) gCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑TATGAAAGAAACTCAAACCGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTAATACCGATATTCAt > 8:34272/1‑138 (MQ=255) acATTGATTTTAAAAAAAAAAATTTA‑AAAATTTATCTCGTTATTAAAAATAAACGTACATAAATT‑TATGAAATAAAATCAACACGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTAATACCGATATTCAt < 4:57862/133‑1 (MQ=255) cATTGCTTTTTATACATAAAATATT‑ATAAATTCTCTCGTTATGAAAAAAAAACGGAGATCAATG‑TATGAAATAAACTCAAACCGCCCGGGAAAAAATAAAACATCTCTTGCACATTTTAATACAGATATTCATTAccc > 7:322296/1‑138 (MQ=255) gCTTTTTATACATAAAATGTT‑ATAATTTCTCTCTTTATAAAAAAGAAACGGAGATAATTT‑TATTAAACAAACTCAAAACGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTAATACCGATATTCATTACCCAAGc < 7:389639/138‑1 (MQ=255) cTTTTTAAACATAAAATGTT‑AAATTTTCTCTCGTTATCAAAAACAACCGGACATCAATG‑TATCAAACAACCTCAAAACGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTAATACCGATATTCATTACCCAAGCgg < 2:23171/139‑1 (MQ=255) aCATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑TATGAAAGAAACTCAAAACGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTAATACCGATATTCATTACCCAAGCGGGGCTTtct > 8:81974/1‑139 (MQ=255) aCATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑TATGAAAGAAACTCAAAACGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTAATACCGATATTCATTACCAAAGCGGGGCtt > 3:405625/1‑136 (MQ=255) aCAAAAAATGTT‑ATAATATCTCTCGTTATTAAAAACAAACCGATATCAATG‑TATCAAACAAACTCAAAACGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTAATACCGATATTCATTACCCAAGCGGGGCtt < 4:214179/136‑1 (MQ=255) tGTT‑ATAATTTCTCTCGTTATGAATAACAATCGGTTATCAATT‑TATGAAAGAAACTAAAAACGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTAATACCGATATTCATTACACAAGCGGGGCTTTCTCTGATCa < 8:322296/138‑1 (MQ=255) | TCGAAAGAATCATTCAGTATATTTGCACCTTTTTAGGCGTGATTTCGCTTTCTTTTATTTATGCCCAAATGTTTAGAAAAACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTT‑ATAATTTCTCTCGTTATGAAAAAGAAACGGAGATCAATG‑TATGAAAGAAACTCAAAACGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTAATACCGATATTCATTACCCAAGCGGGGCTTTCTCTGATCA > CP006881/2071469‑2071741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |