| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 2,643,663 | T→C | 8.5% | intergenic (‑30/+37) | U712_13595 ← / ← U712_13600 | Single‑stranded‑DNA‑specific exonuclease recJ/Uncharacterized protein yrvD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,643,663 | 0 | T | C | 8.5% | 165.4 / 4.1 | 63 | intergenic (‑30/+37) | U712_13595/U712_13600 | Single‑stranded‑DNA‑specific exonuclease recJ/Uncharacterized protein yrvD |
| Reads supporting (aligned to +/- strand): ref base T (21/34); new base C (3/2); total (27/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGCAGGGACGCAACAAGAGGTGTTATATGCAATTGTTCTGTGAGTGATTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCCTCTTGTTTATGTATTGCTGTTCTA > CP006881/2643529‑2643790 | aaGCAGGGACGCAACAAGAGGTGTTATATGCAATTGTTCTGTGAGTGATTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTagag < 5:292103/139‑1 (MQ=255)aaGCAGGGACGCAACAAGAGGTGTTATATGCAATTGTTCTGTGAGTGATTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTagag < 5:96984/139‑1 (MQ=255) gCAGGGACGCAACAAGAGGTGTTATATGCAATTGTTCTGTGAGTGATTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTagag > 2:46199/1‑137 (MQ=255) aGGGACGCAACAAGAGGTGTTATATGCAATTGTTCTGTGAGTGATTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAgg < 1:46199/139‑1 (MQ=255) cGCAACAAGAGGTGTTATATGCAATTGTTCTGTGAGTGATTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAg < 7:372001/139‑1 (MQ=255) aaGAGGTGTTATATGCAATTGTTCTGTGAGTGATTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGttt < 1:411457/139‑1 (MQ=255) gtTATATGCAATTGTTCTGTGAGTGATTTGCCCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGCCGCTAACATAAATTCACCCCACAACCCCCCTATTATACAAGAGCAGGCGGAGGGGTGTCAaataa > 8:331161/1‑139 (MQ=255) gtTATATGCAATTGTTCTGTGAGTGATTTGACCTTATCCTGATCTGGTCGCTGGATTTGCCATCGCATTTTTTACGCTAACATAAAATCACCCCGCAACCCCCCAAATATACTAGACCAGGCGGAGGGGTTTCAaata > 6:111592/1‑138 (MQ=255) gtTATATGCAATTGTTCTGTGAGTGATTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAaata > 8:286491/1‑138 (MQ=255) atatGCAATTGTTATGTGAGTGATTTGACATTATCCTGATCTGGTCGCTGGCTTTACCAACGCATTTTTGAAGCTAACATAAAATAAACCCACAACCCCCCAATTATAACAGAGAATGCATATGGGTTTAAaata > 4:238520/1‑135 (MQ=255) tGCAATTGTTCTGTGAGTGATTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAatat < 3:402113/136‑1 (MQ=255) ttGTTCTGTGAGTGATTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTTACGCTAACATAAATTCACCCCGCAACCACCCTAATATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTAGaaaa > 2:27233/1‑138 (MQ=255) ttGTTCTGTGAGTGATTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGCCGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAAc > 7:276614/1‑139 (MQ=255) tGTTCTGTGAGTGATTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAAATAACCCCTCAACCACCCTATTTTACTAGAGCAGGCTGAGGGGGTTCAAATAATATTAGaa > 4:347633/1‑135 (MQ=255) ttCTGTGAGTGATTTGACCTTATCCTGATCTGGTCGCTGGATTTGCCCGCGCATTTTTTACGCTAACATAAATTAACCCCACAACCACCCAATTATACATGAGCAGGCTGAGGGGGTTCAAAATATATTGGaaaa > 4:73664/1‑135 (MQ=255) ttCTGTGAGTGATTTGACCTTATCCTGATCTGGTCGCTGGATTTACCAAAGCATTTTTGACGCTAACAAAAAATAACCCCTCCACCACCCAATTTTACTAGAGCAAGCTGAGGGGTTTAAAATAATATTAGAAAACTAg > 2:94031/1‑139 (MQ=255) gtgaTTTGACCTTATCCTGATCTGGTCGATTGATTTGCCATACCATTTTTGACGCTAACATAAAATAACACCACAAAACCCCTAATAAACAAGAACAGGCTGATGGGGTTCAAAAAATAATACAAAACTAGTCttt > 4:363958/1‑136 (MQ=255) tgaTTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTtc < 5:300673/139‑1 (MQ=255) tgaTTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTAAACTAGAGCAGGGTGAGGGGTTTCAAATAATATTCTAAAACTAGTCTTTTTtc > 6:208584/1‑139 (MQ=255) aTTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTtct < 7:331161/138‑1 (MQ=255) aTTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTtct < 1:94031/138‑1 (MQ=255) aCCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCa < 4:158349/135‑1 (MQ=255) aTCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATgg < 6:284936/137‑1 (MQ=255) aTCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATgg < 2:195032/137‑1 (MQ=255) aTCTGGTCGCTGGATTTCCCATGGCATTTTTGACGCTAACATAAATTCACCCCGCAACCCCCCTATTATACTAGAGCAGGCTGGGGGGGTTGAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCa > 6:406015/1‑139 (MQ=255) aTCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGaa > 7:405458/1‑106 (MQ=255) aTCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGaa < 8:405458/106‑1 (MQ=255) gTCGCTGGATTTACCATAGCATTTTTTACGCTAACATAAATTCACCCCTCAACCAAACTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATAATCGAAAACTAGTATTTTTTATAAATCATGGCTGATGCATCCtg > 8:96143/1‑139 (MQ=255) gTCGCTGGATTTACCAACGCATTTTTGAAGCTAACAAAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATAATCGAAAACTAGTCTTTTTTCTCAATAATGGCTGATGCATCCtg > 6:375079/1‑139 (MQ=255) cTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCACCCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCtgt < 3:354456/136‑1 (MQ=255) cTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCtgtgtg < 7:286491/139‑1 (MQ=255) cTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCtgtgtg < 2:177733/139‑1 (MQ=255) ccATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGa < 8:276614/139‑1 (MQ=255) cGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGAt < 3:347633/136‑1 (MQ=255) tttGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGAt > 1:229004/1‑139 (MQ=255) ttGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGAt < 5:111592/138‑1 (MQ=255) ttGACGCTAACATAAATTCACCCCTCAACCAAACTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGTCTGATGCATCCTGTGTGTCAGTCTGATCAGCaa < 3:238520/135‑1 (MQ=255) ttGAAGCTAACATAAATTCACCACTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGAt < 5:278407/138‑1 (MQ=255) cTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATc < 5:26012/139‑1 (MQ=255) cTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATc < 5:234520/139‑1 (MQ=255) aCATAAATTCACCCCTCCACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAATTAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCct < 6:221805/138‑1 (MQ=255) cATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCctct < 2:229004/139‑1 (MQ=255) aaaTTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCctct < 3:160436/136‑1 (MQ=255) aaTTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCCTCTTGt < 1:118951/138‑1 (MQ=255) aTTCACCCCTCAACAACCCTATTATACTAGAGCAGGCTGATGGGTTTCAAATAATATTCGTAAACTAGTATTTTTTCTCAATCATGGCTGATGCATGCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCCTCTTGttt < 7:199876/139‑1 (MQ=255) aCCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTGTTCTCAATCATGGCTGATGCATCCTGTGTGGCAGTCTGATCAGCAAGATGAGTATCCTCTTGTTTATg > 8:201986/1‑138 (MQ=255) cccTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCctc > 1:396187/1‑127 (MQ=255) cccTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCctc < 2:396187/127‑1 (MQ=255) cccTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCCTCTTGTTTATGTAt > 1:29126/1‑139 (MQ=255) ccTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCCTCTTGTTTATg > 4:410791/1‑135 (MQ=255) cTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCCTCTTGTTTATGTATTg < 2:89515/139‑1 (MQ=255) cTCAACCACCCTATTATAATAGAGCAGGATGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCCTCTTGTTTATGTa < 3:363958/136‑1 (MQ=255) tCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCaa > 3:153062/1‑111 (MQ=255) tCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCaa < 4:153062/111‑1 (MQ=255) cccTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCCTCTTGt > 8:247159/1‑121 (MQ=255) cccTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCCTCTTGt < 7:247159/121‑1 (MQ=255) cccTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCCTCTTGTTTATGTATTGCTGTTCTa > 7:37206/1‑139 (MQ=255) ttatACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCCTCTTGttt < 5:387876/118‑1 (MQ=255) ttatACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCCTCTTGttt > 6:387876/1‑118 (MQ=255) ttatACCAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGa < 8:77235/104‑1 (MQ=255) ttatACCAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGa < 8:77232/104‑1 (MQ=255) ttatACCAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGa > 7:77232/1‑104 (MQ=255) ttatACCAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGa > 7:77235/1‑104 (MQ=255) tAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATg < 7:335166/66‑1 (MQ=255) tAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATg > 8:335166/1‑66 (MQ=255) tAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCa > 5:220310/1‑68 (MQ=255) tAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCa < 6:220310/68‑1 (MQ=255) tAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGc > 6:205727/1‑91 (MQ=255) tAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGc < 5:205727/91‑1 (MQ=255) | AAGCAGGGACGCAACAAGAGGTGTTATATGCAATTGTTCTGTGAGTGATTTGACCTTATCCTGATCTGGTCGCTGGATTTCCCATCGCATTTTTGACGCTAACATAAATTCACCCCTCAACCACCCTATTATACTAGAGCAGGCTGAGGGGTTTCAAATAATATTCGAAAACTAGTCTTTTTTCTCAATCATGGCTGATGCATCCTGTGTGTCAGTCTGATCAGCAAGATGAGTATCCTCTTGTTTATGTATTGCTGTTCTA > CP006881/2643529‑2643790 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |