Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,791,503 A→G 5.7% S726P (TCA→CCA)  U712_14395 ← DNA polymerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,791,5030AG 5.7% 265.2 / 4.8 86S726P (TCA→CCA) U712_14395DNA polymerase I
Reads supporting (aligned to +/- strand):  ref base A (40/41);  new base G (3/2);  total (43/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

ATAAGCGTTGTTACGTATCCTTTTTGTTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGACATGGAACACATCCATTGCCGTTTTCGTGTGAATATCCATATCAT  >  CP006881/2791367‑2791637
                                                                                                                                        |                                                                                                                                      
aTAAGCGTTGTTACGTATCCTTTTTGTTTCGCGTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACa                                                                                                                                      <  6:99462/139‑1 (MQ=255)
 tAAGCGTTGTTACGTATCCTTTTTGTTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAg                                                                                                                                     <  8:322059/139‑1 (MQ=255)
 tAAGCGTTGTTACGTATCCTTTTTGTTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAg                                                                                                                                     <  6:197198/139‑1 (MQ=255)
 tAAGCGTTGTTACGTATCCTTTTTGTTTCGCTGCCTGTACTGAATCTTCCATGAACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAg                                                                                                                                     <  8:327590/139‑1 (MQ=255)
      gttgttACGTATCCTTTTTGTTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCg                                                                                                                                 >  5:140287/1‑138 (MQ=255)
       ttgttACGTATCCTTTTTGTTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGt                                                                                                                                >  7:259913/1‑138 (MQ=255)
       gtgttACGTATCCTTTTTGTTTCTCTTCCTGTACTGAATCTTCCATGTACTCTTTTACGCCTTGGAATCGTTCCAGATAGAGTTCGATAAATGATACCGCGTCAGTCCGGGTAATGCAGAGGTTCTGTGACATAcc                                                                                                                                  >  3:21817/2‑136 (MQ=255)
              gTATCCTTTTTGTTTCGGTTGCTTTACTGAATCTTCCATGTACGCTTTTACTCCTTGGAAGCTTTCGAGATAGCGGTCGATAATTGCTGGCGCTTCCTTCCGGGTAATGCCGAGGTTGTGTGAAAGACCGTAATCg                                                                                                                           <  4:287331/136‑1 (MQ=255)
              gTATCCTTTTTGTTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTg                                                                                                                        <  8:207640/139‑1 (MQ=255)
              gTATCCTTTTTGTTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTg                                                                                                                        <  5:357161/139‑1 (MQ=255)
              gTATCCTTTTTGGTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTg                                                                                                                        <  5:104041/139‑1 (MQ=255)
               tATCCTTTTTGTTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTg                                                                                                                        <  1:324464/138‑1 (MQ=255)
               tATCCTTTTTGTTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTg                                                                                                                        >  2:324464/1‑138 (MQ=255)
               tATCCTTTTTGTTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTg                                                                                                                        >  2:372002/1‑138 (MQ=255)
               tATCCTTTTTGTTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGa                                                                                                                       >  7:366144/1‑139 (MQ=255)
               tATCCTTTTTGTTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGa                                                                                                                       >  1:2303/1‑139 (MQ=255)
                  ccTTTTTGTTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACGGACCGGAATCGCTGa                                                                                                                       >  4:411406/1‑136 (MQ=255)
                  ccTTTTTGTTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATcc                                                                                                                    >  2:259671/1‑139 (MQ=255)
                        tGTTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCGTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTaaa                                                                                                              >  7:86133/1‑139 (MQ=255)
                          tttCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAAc                                                                                                             <  5:111380/138‑1 (MQ=255)
                          tttCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAAc                                                                                                             <  1:372002/138‑1 (MQ=255)
                              gCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAAGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACaa                                                                                                           <  3:200710/136‑1 (MQ=255)
                                        cTGAATCTTCCCTGTACGCTGTTACGCCTTGGAAGCTTTCCAGCTAGCGGTCGCTCAATGCTCCCGCTTCCTTCCGGGTAATGCCTAGGTTCTGTGAAAGACCGTAATCGATGATCCCGTAACACCTGCCAAAGtt                                                                                                 <  4:21817/136‑1 (MQ=255)
                                          gAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTg                                                                                                                        >  2:412195/1‑111 (MQ=255)
                                          gAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTg                                                                                                                        <  1:412195/111‑1 (MQ=255)
                                          gAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCg                                                                                            >  6:379204/1‑139 (MQ=255)
                                             tCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGCCCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCtt                                                                                         >  8:337838/1‑139 (MQ=255)
                                             tCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTg                                                                                                                        >  7:238772/1‑108 (MQ=255)
                                             tCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTg                                                                                                                        <  8:238772/108‑1 (MQ=255)
                                                tCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCg                                                                                      >  7:301025/1‑139 (MQ=255)
                                                   aTGTACGCTTTTACGCATTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGCTAATGCCGAGTTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAAAGCCAAAGTTGACCGCTTTCGc                                                                                     >  2:331291/1‑137 (MQ=255)
                                                          cTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTACTGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGtct                                                                            <  1:268449/139‑1 (MQ=255)
                                                          cTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGtct                                                                            <  6:334614/139‑1 (MQ=255)
                                                           ttttACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGtct                                                                            >  2:268449/1‑138 (MQ=255)
                                                           ttttACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGtct                                                                            >  2:267106/1‑138 (MQ=255)
                                                              tACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATc                                                                        >  7:371570/1‑139 (MQ=255)
                                                                 gCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATc                                                                        <  4:148993/136‑1 (MQ=255)
                                                                     tGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGGCAGACCGTAATCGCTGATCCCGt                                                                                                                 <  8:88081/91‑1 (MQ=255)
                                                                     tGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGGCAGACCGTAATCGCTGATCCCGt                                                                                                                 <  8:94129/91‑1 (MQ=255)
                                                                     tGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGGCAGACCGTAATCGCTGATCCCGt                                                                                                                 >  7:88081/1‑91 (MQ=255)
                                                                     tGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGGCAGACCGTAATCGCTGATCCCGt                                                                                                                 >  7:94129/1‑91 (MQ=255)
                                                                     tGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATg                                                                 >  8:7288/1‑139 (MQ=255)
                                                                     tGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATg                                                                 >  5:56246/1‑139 (MQ=255)
                                                                     tGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATg                                                                 >  5:256218/1‑139 (MQ=255)
                                                                      ggAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAg                                                                    <  3:253322/135‑1 (MQ=255)
                                                                      ggAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATg                                                                 <  7:7288/138‑1 (MQ=255)
                                                                      ggAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATg                                                                 <  8:371570/138‑1 (MQ=255)
                                                                           cTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCa                                                              <  4:134539/136‑1 (MQ=255)
                                                                           cTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCa                                                              <  4:199810/136‑1 (MQ=255)
                                                                           cTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACtt                                                           <  8:366144/139‑1 (MQ=255)
                                                                             ttCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACtt                                                           >  5:389260/1‑137 (MQ=255)
                                                                             ttCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACtt                                                           <  6:389260/137‑1 (MQ=255)
                                                                             ttCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTcc                                                         >  5:36887/1‑138 (MQ=255)
                                                                              tCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCg                                                                                                                           <  4:35675/72‑1 (MQ=255)
                                                                              tCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCg                                                                                                                           >  3:35675/1‑72 (MQ=255)
                                                                                    tAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAg                                                  <  8:301025/139‑1 (MQ=255)
                                                                                    tAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAg                                                  <  5:379204/139‑1 (MQ=255)
                                                                                     aGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGGCAGCCCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCGAATGGCCGCTGTCACGTCATCCTTAGc                                                 >  2:413878/1‑139 (MQ=255)
                                                                                     aGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGCCCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTACCTTCATCCtt                                                    >  4:423423/1‑136 (MQ=255)
                                                                                     aGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATg                                                                 <  1:399755/123‑1 (MQ=255)
                                                                                     aGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATg                                                                 >  2:399755/1‑123 (MQ=255)
                                                                                      gCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCACCTCGCAGATGTGCCTTCAACCTTAGc                                                 >  2:414589/1‑138 (MQ=255)
                                                                                       cGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGa                                               >  7:206579/1‑139 (MQ=255)
                                                                                        ggTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGc                                                 >  4:405698/1‑136 (MQ=255)
                                                                                         gTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGACa                                             <  8:206579/139‑1 (MQ=255)
                                                                                         gTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGACa                                             <  1:259671/139‑1 (MQ=255)
                                                                                         gTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGACa                                             <  6:140287/139‑1 (MQ=255)
                                                                                          tCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGa                                               >  4:337274/1‑136 (MQ=255)
                                                                                          tCGATAAATGCTCCAGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGGAAACAATGCCAAAGTTGTCCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTGATCCTTAGCGACAt                                            <  1:331291/139‑1 (MQ=255)
                                                                                            gATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACATACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCAATTCATCCTTAGCGACATg                                           <  1:419751/138‑1 (MQ=255)
                                                                                                 aTGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGACATGGAacac                                     >  8:374038/1‑139 (MQ=255)
                                                                                                    cTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCCGATGTCACTTCATCCTTAGCGACATGGAACACATc                                  >  6:351978/1‑139 (MQ=255)
                                                                                                                  cGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCTAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTATCGACATGGAACACATCCATTTCCGTTTTCg                    <  8:259913/139‑1 (MQ=255)
                                                                                                                  cGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGACATGGAACACATCCATTGCCGTTTTc                     <  1:267106/138‑1 (MQ=255)
                                                                                                                   gggTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGACATGGAACACATCCATTGCCGTTTTCgt                   >  8:263632/1‑139 (MQ=255)
                                                                                                                       aaTGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATTGCAGATGTCACTTCATCCTTAGCGACATGGAACACATCCATTGCCGTTTTCgtg                  >  3:293239/1‑136 (MQ=255)
                                                                                                                        aTGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGACATGGAACACATCCATTGCCGTTTTCGTGTGaa              <  7:374038/139‑1 (MQ=255)
                                                                                                                        aTGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGACATGGAACACATCCATTGCCGTTTTCGTGTGaa              <  1:330218/139‑1 (MQ=255)
                                                                                                                        aTGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGACATGGAACACATCCATTGCCGTTTTCGTGTGaa              >  2:67848/1‑139 (MQ=255)
                                                                                                                        aTGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGACATGGAACACATCCATTGCCGTTTTCGTGTGaa              <  7:275893/139‑1 (MQ=255)
                                                                                                                        aTGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCATTAGCGACATGGAACACATCCATTGCCGTTTTCGTGTGaa              <  6:56246/139‑1 (MQ=255)
                                                                                                                         tGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGCCATGGAACACATCCATTGCCGTTTTCGTGTGaa              >  6:355800/1‑138 (MQ=255)
                                                                                                                              aGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGACATGGAACACATCCATTGCCGTTTTCGTGTGAATATCCa        <  6:379125/139‑1 (MQ=255)
                                                                                                                              aGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGACATGGAACACATCCATTGCCGTTTTCGTGTGAATATCCa        >  5:162342/1‑139 (MQ=255)
                                                                                                                                   cTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCACAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGCTGTCACTTCATCCTTAGCGCCATGGAACCCATCCATTTCCGTTTGCGTTGGAATATCCAGATCa   >  1:40081/1‑139 (MQ=255)
                                                                                                                                    tgtgACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGACATGGAACACATCCATTGCCGTTTTGGTGTGAATATCAATATCAt  >  7:167861/1‑139 (MQ=255)
                                                                                                                                        aCAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGACATGGAACACATCCATTGCCGTTTTCGTGTGAATATCCATATCAt  >  4:291759/1‑135 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ATAAGCGTTGTTACGTATCCTTTTTGTTTCGCTTCCTGTACTGAATCTTCCATGTACGCTTTTACGCCTTGGAAGCTTTCCAGATAGCGGTCGATAAATGCTCCCGCTTCCTTCCGGGTAATGCCGAGGTTCTGTGACAGACCGTAATCGCTGATCCCGTAAACAATGCCAAAGTTGACCGCTTTCGCCTGGCGTCTCATCGCAGATGTCACTTCATCCTTAGCGACATGGAACACATCCATTGCCGTTTTCGTGTGAATATCCATATCAT  >  CP006881/2791367‑2791637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑