Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	2,991,169	A→T	53.9%	intergenic (‑5/+13)	U712_21340 ← / ← U712_21345	tRNA‑Leu/tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,991,169	0	A	T	53.9%	1.3 / 7.7	20	intergenic (‑5/+13)	U712_21340/U712_21345	tRNA‑Leu/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base A (3/6);  new base T (6/3);  total (11/9)
Fisher's exact test for biased strand distribution p-value = 3.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGAGCGGAAGACGGGATTCGAACCCGCGACCCCCACCTTGGCAAGGTGGTGTTCTACCACTGAACTACTTCCGCAGAAATGGTGCGGATGAAGGGACTTGAACCCCCACGTCTGT  >  CP006881/2991031‑2991294                                                                                                                                           |                                                                                                                              tCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCAGCCCGGCGTGGa                                                                                                                               <  8:113746/139‑1 (MQ=17) tCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                                                                                                               <  1:396226/139‑1 (MQ=32) tCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                                                                                                               <  1:425143/139‑1 (MQ=32) tCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                                                                                                               <  6:328998/139‑1 (MQ=32)  ccGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa                                                                                                                               <  6:73050/138‑1 (MQ=32)    gTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCgtgggtgg                                                                                                                            >  1:364851/1‑139 (MQ=17)    gTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATgg                                                                                                                            >  5:293955/1‑139 (MQ=32)               ctctATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGGTGGTTTATTTgggg                                                                                                                 >  8:162684/1‑137 (MQ=255)                  tATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGGCTAGTTGGGGCg                                                                                                               >  7:113896/1‑138 (MQ=12)                      cAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGAGCGGAAGa                                                                                                          <  7:174661/139‑1 (MQ=32)                      cAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGAGCGGAAGa                                                                                                          <  6:229458/139‑1 (MQ=32)                        aTTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACATACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCTGCTTGGTTGGTTTAGTTGGAGCGGCAGACg                                                                                                        >  8:12500/1‑139 (MQ=11)                         ttGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTCCCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGTTGGTTTTGTTGGAGCGGAAGACgg                                                                                                       >  8:118368/1‑139 (MQ=255)                          tGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGTTTGTATTTTTTGAGCGGAAGACggg                                                                                                      >  6:409756/1‑139 (MQ=11)                           gAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCCTGGTTTCTATTGTTTGAACGGAAAACg                                                                                                        >  4:366281/1‑136 (MQ=11)                            aGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGTAGGGTTAGTTGGAGGGGATGCCgggtt                                                                                                    >  6:366894/1‑137 (MQ=11)                            aGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGAGCGGAAGACGGGAt                                                                                                    >  7:277313/1‑139 (MQ=32)                              cTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGGTGGTTTAGTTGGAGCGGAAGACGGGGttg                                                                                                  >  1:10428/1‑138 (MQ=12)                                     cgcAAAACTTAATCCCGATGGCCGGACTTCAACCGGCACGGTAGTCACCTACCGCCGGATTTAAAGTCCGGGGTGTCTGCCACTGCCACCACCCCGGCTGGGTTTGTATAGTTGGTGGGGAAGCCGGGATTCGAAc                                                                                              <  4:129001/136‑1 (MQ=11)                                      gcAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGTTGGGATTTTTGGATGGGAAGACGGGGTTCCAACCcgc                                                                                          >  7:323941/1‑139 (MQ=11)                                                         gcgggACTTGACCCGTCAGGGTAGTACCCTACCGCTGTTTTTTAAGTCGTGTGTGTGTGCCATTCCCACCCCCCGGGGGTGTTTGTTATAGTTGGTGGGTACCAGGGGATTCCAACCCGCGACCCCCACCTTGGCAAgg                                                                       <  8:99668/136‑1 (MQ=0)                                                                                              gATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGCGCGGAAGACGGGATTCGAACCCGCGACCCCCACCTTGGCAAGGTGGTGTTCTACCACTGAACTACTTCCGCAGAAATGGt                                  <  7:276160/139‑1 (MQ=21)                                                                                                        cgtgtgtgTCTGCCAATACCACCACCCGGTCGTGTTTGTTAAAGTGGCAGCGGAAGACGGGATACCACCCCGCGACCCCCACCTTGGCAAGGTGGTGTTCTACCACTGAACTACTTCCGCAGAAATGGTGCGGATGAAg                        <  5:366894/137‑1 (MQ=14)                                                                                                              tgtcgcccATTTCCCCCACCCGGGCTTGTATTGTATAGTTGGCGGGTAAGACGGGATTCGAACCCGCGACCCCCACCTTGGCAAGGTGGTGTTCTACCACTGAACTACTTCCGCAGAAATGGTGCGGATGAAGGGACtt                  <  7:404944/133‑1 (MQ=17)                                                                                                                       atccccccaccCCGGCGTGGATGTTATAGTTGGCGCGGAAGCCGGGATTCCAACCCGCGACCCCCACCTTGGCAAGGTGGTGTTCTACCACTGAACTACTTCCGCAGAAATGGTGCGGATGAAGGGACTTGAACCCCCa         <  8:395293/133‑1 (MQ=17)                                                                                                                              caccCGGGCGTGGATGGTATAGTTGGCGCGGAAGACGGTATTCGAACCCGCGACCCCCACCTTGGCAAGGTGGTGTTCTACCACTGAACTACTTCCGCAGAAATGGTGCGGATGAAGGGACTTGAACCCCCACGTCTGt  <  8:323941/139‑1 (MQ=255)                                                                                                                                           |                                                                                                                              TCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGAGCGGAAGACGGGATTCGAACCCGCGACCCCCACCTTGGCAAGGTGGTGTTCTACCACTGAACTACTTCCGCAGAAATGGTGCGGATGAAGGGACTTGAACCCCCACGTCTGT  >  CP006881/2991031‑2991294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑