Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,299,347 A→G 6.4% intergenic (‑112/+205) U712_16975 ← / ← U712_16980 Phosphoglycerate kinase/Glyceraldehyde‑3‑phosphate dehydrogenase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,299,3470AG 6.4% 242.7 / 6.1 80intergenic (‑112/+205)U712_16975/U712_16980Phosphoglycerate kinase/Glyceraldehyde‑3‑phosphate dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base A (36/38);  new base G (2/3);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

ATGTCTTTGAGAGTTTTTTTATTCATGCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATCACAGAGCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCT  >  CP006881/3299210‑3299482
                                                                                                                                         |                                                                                                                                       
aTGTCTTTGAGAGTTTTTTTATTCATGCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAg                                                                                                                                        >  7:358823/1‑139 (MQ=255)
 tggcTTTGAGAGTTTTTTTATTCATGCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGGTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCGGt                                                                                                                                       <  7:87009/136‑1 (MQ=255)
 tGTCTTTGAGAGTTTTTTTATTCATGCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGt                                                                                                                                       <  8:69954/139‑1 (MQ=255)
   tCTTTGAGAGTTTTTTTATTCATGCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTc                                                                                                                                     <  5:277790/139‑1 (MQ=255)
         cgagCTTGTTTATTCAAGCCTAGGAGATCCTCCTTCTAAACTGCTTGCCTACAGCACGACACACGGGAAACCGTCTGTGCAAGCGATATCTCCGCTAGGGCAGATCGCAGTCTTTATCGGCACAGGGCAGTTCGTCTa                                                                                                                                <  2:366674/137‑1 (MQ=255)
         agagTTTTTTTATTGATGACTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAAGCGTCTGTTCAATCGATCACTCCGCTAGGGCAGTTCGGAGTGTTTTTCGGCTCAGTGCAGTTCGt                                                                                                                                   <  3:128907/135‑1 (MQ=255)
         agagTTTTTTTATTCATGCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTa                                                                                                                                <  5:363099/138‑1 (MQ=255)
             tttttttATTCATGCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGc                                                                                                                           <  1:358422/139‑1 (MQ=255)
             tttttttATTCATGCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGc                                                                                                                           <  8:1123/139‑1 (MQ=255)
             tttttttATTCATGCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGc                                                                                                                           >  8:54713/1‑139 (MQ=255)
                     ttCATGCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAg                                                                                                                   <  7:319494/139‑1 (MQ=255)
                     ttCATGCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAg                                                                                                                   <  2:389958/139‑1 (MQ=255)
                     ttCATGCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAg                                                                                                                   <  6:118861/139‑1 (MQ=255)
                     ttCATGCCTAGGAGATCCTCCTTCAAAATGGTATGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAgg                                                                                                                       <  3:266807/135‑1 (MQ=255)
                       cATGCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGc                                                                                                                  <  8:358823/138‑1 (MQ=255)
                       cATGCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCg                                                                                                                 >  1:205355/1‑139 (MQ=255)
                          gCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTaaa                                                                                                                              <  4:313640/123‑1 (MQ=255)
                          gCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTaaa                                                                                                                              >  3:313640/1‑123 (MQ=255)
                                    tcctccTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGaaa                                                                                                    >  6:116028/1‑139 (MQ=255)
                                    tcctccTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGa                                                                                                      >  6:418954/1‑137 (MQ=255)
                                     cctcctTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAg                                                                                                   >  6:194609/1‑139 (MQ=255)
                                     cctcctTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAg                                                                                                   >  6:201193/1‑139 (MQ=255)
                                       tcctTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGa                                                                                                      >  3:182994/1‑134 (MQ=255)
                                       tcctTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGGTCAAGCGATACCTCCGCTAGGGCAGATCGCAGTCTGTTTCGGCACAGGGCAGTTCGTCTCAAAGCCAGGGCAGCGGCCTTCGGCGaag                                                                                                    >  4:49501/1‑135 (MQ=255)
                                               aaTGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCa                                                                                           >  5:276275/1‑137 (MQ=255)
                                                aTGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAg                                                                                        <  7:54713/139‑1 (MQ=255)
                                                  ggTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGATAGCGCCGGCa                                                                                           >  3:85250/1‑134 (MQ=255)
                                                      tGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAgctgct                                                                                   >  8:332931/1‑138 (MQ=255)
                                                      tGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGCGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAgctgct                                                                                   >  2:213038/1‑138 (MQ=255)
                                                             aGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGGAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAAcgc                                                                           >  7:42300/1‑139 (MQ=255)
                                                             aGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAActt                                                                           >  5:422935/1‑137 (MQ=255)
                                                             aGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAAcgc                                                                           >  1:291646/1‑139 (MQ=255)
                                                              gCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCGGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAAcgcg                                                                          >  7:39232/1‑139 (MQ=255)
                                                              gCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAg                                                                                                                   >  2:159156/1‑98 (MQ=255)
                                                              gCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAg                                                                                                                   <  1:159156/98‑1 (MQ=255)
                                                              gCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAActcg                                                                          >  2:170778/1‑139 (MQ=255)
                                                              gCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAAcgcg                                                                          >  6:327838/1‑139 (MQ=255)
                                                                aCGAAAAACGTGAAACCGTCTGTTCTAGCGATAACTCCGGTAGGGCAGATCGCAGGCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCTGGGCAGCTGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGt                                                                        <  2:295527/139‑1 (MQ=255)
                                                                aCGAAAAACGGGAAACGGTCTGTTCAAGCGATAACTCCGCTCGGGCAGATCGCTGTCTTTTTCGGCACAGGGCAGTTCGTCTAAACGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGt                                                                        <  6:276275/139‑1 (MQ=255)
                                                                 cGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAAcgcg                                                                          >  3:231488/1‑136 (MQ=255)
                                                                 cGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAAcgcg                                                                          >  3:119438/1‑136 (MQ=255)
                                                                  gAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCGGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCg                                                                      >  2:89511/1‑139 (MQ=255)
                                                                  gAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCg                                                                      >  6:158043/1‑139 (MQ=255)
                                                                   aaaaaCGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCGGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGt                                                                     <  1:239081/139‑1 (MQ=255)
                                                                   aaaaaCGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGt                                                                        <  4:182994/136‑1 (MQ=255)
                                                                       aggTGAAACTGTCGGTTCAATCGATAACTCCGCTAGGGAAGATCGCAGTCTTTTTCGGCACAGGGCTGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGtt                                                                    <  4:410437/134‑1 (MQ=255)
                                                                         gTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGt                                                                     <  5:368852/133‑1 (MQ=255)
                                                                         gTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGt                                                                     >  6:368852/1‑133 (MQ=255)
                                                                         gTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTctct                                                               >  8:232870/1‑139 (MQ=255)
                                                                              aCCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTAt                                                             <  4:231488/136‑1 (MQ=255)
                                                                                cGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATca                                                           <  4:134015/136‑1 (MQ=255)
                                                                                cGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATCACag                                                        <  8:42300/139‑1 (MQ=255)
                                                                                  tCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATcac                                                          >  7:225652/1‑135 (MQ=255)
                                                                                  tCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATcac                                                          <  8:225652/135‑1 (MQ=255)
                                                                                   cTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTGTCTCGATCACAGAGc                                                     >  1:300610/1‑139 (MQ=255)
                                                                                         aaGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAg                                                                                                                   >  8:407919/1‑71 (MQ=255)
                                                                                         aaGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAg                                                                                                                   <  7:407919/71‑1 (MQ=255)
                                                                                           gCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGtt                                                                                                                                      >  2:336019/1‑50 (MQ=255)
                                                                                           gCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGtt                                                                                                                                      <  1:336019/50‑1 (MQ=255)
                                                                                              aTAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTGTGTCTATCACAGCGCGGTTTATGaaa                                          >  6:105079/1‑139 (MQ=255)
                                                                                                     cGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCGGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATCACAGAGCGTTTTCTGAAAGCCGGTc                                   <  8:39232/139‑1 (MQ=255)
                                                                                                     cGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTGTCGCTATCACAGCGCGGTTTGTGAAAGGCGGTc                                   >  8:8151/1‑139 (MQ=255)
                                                                                                     cGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTGTATCTAAAACAGAGCGTTTTCTGAAAGCCgga                                    >  6:391422/1‑137 (MQ=255)
                                                                                                       cTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTGTCTCTATCACAGAGCGTTTTCTGAAAGGCGGTCtt                                 >  8:278060/1‑139 (MQ=255)
                                                                                                          gggCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAACGCCCGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATCACAGAGCGTTTTCTGAAAGCCGGTCTTCa                               <  1:213038/138‑1 (MQ=255)
                                                                                                          gggCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATCACAGAGCGTTTTCTGAAAGCCGGTCTTCa                               <  7:332931/138‑1 (MQ=255)
                                                                                                               gATCGCAGTTTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATCACAGAGCGTTTTCTGAAAGCCGGTCTTCATGGaaa                         <  5:116028/139‑1 (MQ=255)
                                                                                                                          ttttCGGCAAAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCTCAGGCAAAGCTGCTTAAAACGCGGTCGTGTGCCTAACAAAGATCTGTTTATTGAAGACCGTGTTCATTGAAaaaccgaaacgg              >  8:26375/1‑139 (MQ=255)
                                                                                                                              cGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATCACAGAGCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAgt           <  8:1921/138‑1 (MQ=255)
                                                                                                                               ggCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTGTCTGTTTCACAGCGCGGTTTGTTAATGCCGGTCGTCATGGAAAAATCGTAAAGGAgtcc         >  2:233189/1‑139 (MQ=255)
                                                                                                                                gCACCGGGCAGTTCGTCTAAACGCCTGGGCCGGGGCCTTAGGCTAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATCACAGAGCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAgtccg        <  2:205355/139‑1 (MQ=255)
                                                                                                                                gCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATCACAGAGCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAgtccg        <  5:327838/139‑1 (MQ=255)
                                                                                                                                gCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATCACAGAGCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAgt           <  4:119438/136‑1 (MQ=255)
                                                                                                                                 cacaGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTTTAACAGAGCGTTTTATGAAAGCCGGTCTTCAAGGAAAAACGGAAAGGGTgtccgt       >  5:372629/1‑139 (MQ=255)
                                                                                                                                  acaGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATCACAGAGCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAgtccgtc      <  5:158043/139‑1 (MQ=255)
                                                                                                                                    agtgCAGTTCGTCTAAAAGCAAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCATAGCTGCTTAAAACGCGGTCGTTTCTCTATCACAGAGCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAgtccgt       <  3:425143/133‑1 (MQ=255)
                                                                                                                                    aGGGCAGTTCGGCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATCACAGAGCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAgtccgt       <  4:68413/136‑1 (MQ=255)
                                                                                                                                     gggCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATCACAGAGCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTcccc   <  5:418954/139‑1 (MQ=255)
                                                                                                                                     gggCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTGTCTCTATCACAGAGCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTcccc   <  2:300610/139‑1 (MQ=255)
                                                                                                                                      ggCAGCTCGTCTATAAGCCAGGGCAGCGGCGTTAGGAGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATCACAGAGCGTTTTCTTAAAGCCGGGCTTCATTGAAAAAGGGAAAGGGAGTCCGTCccct  <  1:313928/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
ATGTCTTTGAGAGTTTTTTTATTCATGCCTAGGAGATCCTCCTTCAAAATGGTTTGCATACAGCACGAAAAACGTGAAACCGTCTGTTCAAGCGATAACTCCGCTAGGGCAGATCGCAGTCTTTTTCGGCACAGGGCAGTTCGTCTAAAAGCCAGGGCAGCGGCCTTAGGCGAAAGCGCCGGCAAAGCTGCTTAAAACGCGGTCGTTTCTCTATCACAGAGCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCT  >  CP006881/3299210‑3299482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑