Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,321,905	G→A	6.1%	L344L (CTG→TTG)	U712_17080 ←	Beta‑galactosidase ganA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,321,905	0	G	A	6.1%	216.5 / 2.9	67	L344L (CTG→TTG)	U712_17080	Beta‑galactosidase ganA
Reads supporting (aligned to +/- strand):  ref base G (29/33);  new base A (2/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01

TTGAAAGACGCGGTTCTTCGGGCTATTGTCATGATCCACAACCGCTCCGTGTAATTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCAAGCTTCGGTACAGATCATTGATAAAGCCGACCTTCATCGCC  >  CP006881/3321768‑3322038                                                                                                                                          |                                                                                                                                      atGACAGACGCGGGTCTTCGGGCTATTGTCATGATCCACAACCGCTCCGTGTAATTTTTCTGATGACCCCCGGTATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGAcagc                                                                                                                                      <  7:223014/138‑1 (MQ=255)   gAAAGACGCGGTTCTTCGGGCTATTGTCATGATCCACAACCGCTCCGTGTAATTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGAcagca                                                                                                                                     <  7:24691/138‑1 (MQ=255)        aCGCGGTTCTTCGGGCTATTGTCATGATCCACAACCGCTCCGTGTAATTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCa                                                                                                                               >  2:240266/1‑139 (MQ=255)        aCGCGGTTCTTCGGGCTATTGTCATGATCCACAACCGCTCCGTGTAATTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCa                                                                                                                               <  1:240266/139‑1 (MQ=255)               tcttcGGGCTATTGTCATGATCCACAACCGCGCCGTGTAATTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCgg                                                                                                                        <  6:141180/139‑1 (MQ=255)                   cGGGCTATTGTCATGATCCACAACCGCTCCGTGTAATTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGgcg                                                                                                                     <  2:13049/138‑1 (MQ=255)                        tATTGTCATGATCCACAACCGCTCCGTGTAATTTTTCTGATGAACCCCGTGATTTTCGGGATTGGAAATAGAGAACGCTGTCCGAGCCCTGGGCAAACAATTGGAGGGATGACACCAGATTACTTCCCGGGCGCGTTGc                                                                                                               >  2:21386/1‑139 (MQ=255)                          ttGTCATGATCCACAACCGCTCCGTGTAATTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGc                                                                                                               <  5:257487/137‑1 (MQ=255)                                  aTCCACAACCGCTCCGTGTAATTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACg                                                                                                     >  1:67259/1‑139 (MQ=255)                                  aTCCACAACCGCTCCGTGTAATTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGGGGGCCATCATTTGCATGGTTGACAGCAGATTCATTCCCGGGCGCTGTGCCGTGTTGagg                                                                                                     >  2:97770/1‑137 (MQ=255)                                     cacaACCGCTCCGTGTATTTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCACGCCCGGGCGCTTTGCCCTGTTGACg                                                                                                     <  4:327733/136‑1 (MQ=255)                                     cacaACCGCTCCGTGTAATTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACg                                                                                                     >  4:208926/1‑136 (MQ=255)                                             cTCCGTGTAATTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTTCATGGATGACAGCAGATTCATGCCCGGGCGCGTTGCCGTGTTTACGTTATTCCAAtt                                                                                          >  7:23592/1‑139 (MQ=255)                                             cTCCGTGTAATTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCGTTGCCGTGTTGACGTTATTCCAAtt                                                                                          >  6:72174/1‑139 (MQ=255)                                                cGTGTAATTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCCGATTCATGCCCGGGCGCTTTGCCGTGTTGACGTTTTGGCaaat                                                                                          >  4:333638/1‑134 (MQ=255)                                                cGTGTAATTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGAc                                                                                       <  1:202398/139‑1 (MQ=255)                                                     aaTTTTTCTGATGAACCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTTTCCGAGCCGTGGGCAATCATTTGAATGGATGAAAGCAGATTCATGACCGGGCGCTTTGCCGTGTTGCAGTTATTCCAATTGACcg                                                                                     >  3:172783/1‑136 (MQ=255)                                                      aTTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACcg                                                                                     <  3:124971/135‑1 (MQ=255)                                                      aTTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCgcgc                                                                                  <  6:66821/138‑1 (MQ=255)                                                            cTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCg                                                                            >  8:119595/1‑138 (MQ=255)                                                               atgcCCCCCGTGATTAGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTa                                                                          <  1:109705/137‑1 (MQ=255)                                                               atgaCCCCCGTGATTTGCGGTATTGGAAATAGAGACCGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCGTTGCCTTGTTGACGGTATGCCAATTGACCGCGCTTGGCg                                                                            >  3:328127/1‑135 (MQ=255)                                                               atgaCCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTaca                                                                        >  2:2856/1‑139 (MQ=255)                                                               atgaCCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTaca                                                                        <  2:261616/139‑1 (MQ=255)                                                               atgaCCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTa                                                                          <  1:247235/137‑1 (MQ=255)                                                               atgaCCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTa                                                                          <  5:414091/137‑1 (MQ=255)                                                               atgaCCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTa                                                                          <  5:298203/137‑1 (MQ=255)                                                               atgaCCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTa                                                                          <  1:215467/137‑1 (MQ=255)                                                               atgaCCACCGTGATTAGCCGGATTGGAAATAGAGCACGCTGGCCGAGCCGTGGGCAATCATTTGCATGGAGGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCTAGCTTGGCGTaca                                                                        <  6:350403/139‑1 (MQ=255)                                                                tgaCCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTaca                                                                        <  8:131134/138‑1 (MQ=255)                                                                  aCCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTc                                                                     <  1:2856/139‑1 (MQ=255)                                                                       cGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCAtt                                                                 >  6:7348/1‑138 (MQ=255)                                                                       cGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATta                                                                <  2:225079/139‑1 (MQ=255)                                                                              tGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATaag                                                           >  8:257219/1‑137 (MQ=255)                                                                                cGGTTTTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACTTTATGCCAATTGACCGCGCTTGTCGTACACTCCATTAATaaga                                                          <  4:172783/136‑1 (MQ=255)                                                                                     ttGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGgctgc                                                  >  5:176338/1‑139 (MQ=255)                                                                                     ttGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGgctg                                                   <  1:110529/138‑1 (MQ=255)                                                                                     ttGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCGTTGCCTTGTTGACGTTTTGCCAATTGACCGCGCTTGGCGTACCCTCCATTAATAAGAAGGgctgc                                                  >  8:124640/1‑139 (MQ=255)                                                                                     ttGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAACAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATaagaag                                                        <  8:93002/133‑1 (MQ=255)                                                                                     ttGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAACAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATaagaag                                                        <  8:86774/133‑1 (MQ=255)                                                                                     ttGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAACAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATaagaag                                                        >  7:93002/1‑133 (MQ=255)                                                                                     ttGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAACAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATaagaag                                                        >  7:86774/1‑133 (MQ=255)                                                                                            tAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGggtgctgct                                              <  3:208926/136‑1 (MQ=255)                                                                                            tAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCa                                           <  6:176338/139‑1 (MQ=255)                                                                                             agagAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCGTTGCCTTGTTGACGTTTTGCCAATTGCCCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTc                                            >  7:104544/1‑137 (MQ=255)                                                                                             agagAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCGTTGCCGTGTTGACGTTTTGCCAATTGGCCGCGCTTGGCGGACCCTCCATTAATAAGAAGGggtgctgggtg                                            >  1:424292/1‑133 (MQ=255)                                                                                                 accGCTGTCCGAGCCGTTGGCAATCATTTGCATGGAGGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCGTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCAAGCtt                                      <  6:264160/137‑1 (MQ=255)                                                                                                 aaCGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGt                                                                                                    >  1:32722/1‑77 (MQ=255)                                                                                                 aaCGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGt                                                                                                    <  2:32722/77‑1 (MQ=255)                                                                                                 aaCGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCAAGCtt                                      <  1:223906/139‑1 (MQ=255)                                                                                                 aaCGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCAAGCtt                                      <  1:97770/139‑1 (MQ=255)                                                                                                 aaCGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCGTGTTGACGTTATGCCAATTGACCGCGCCTGGCGTACACTCCATTAATAAGACGGTCTGCTGCGTCAAGCtt                                      <  2:305467/139‑1 (MQ=255)                                                                                                 aaCGCCGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGGTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCAAg                                         <  4:10772/136‑1 (MQ=255)                                                                                                  aCGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCAAGCTTc                                     >  7:273201/1‑139 (MQ=255)                                                                                                   cGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATTCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCAAGCTTCg                                    >  5:215743/1‑139 (MQ=255)                                                                                                   cGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCAAGCTTCg                                    <  6:194551/139‑1 (MQ=255)                                                                                                    gCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTa                                                                          >  6:422241/1‑100 (MQ=255)                                                                                                    gCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTa                                                                          <  5:422241/100‑1 (MQ=255)                                                                                                        tCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCAAGCTTCGGTa                                 >  5:145703/1‑137 (MQ=255)                                                                                                            aGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGa                                                                                                       >  3:134627/1‑63 (MQ=255)                                                                                                            aGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGa                                                                                                       <  4:134627/63‑1 (MQ=255)                                                                                                             gCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCAAGCTTCGGTACAGATCa                          >  1:1901/1‑139 (MQ=255)                                                                                                                gTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCAAGCTTCGGTACAGATCa                          >  3:339595/1‑136 (MQ=255)                                                                                                                  gggCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCAAGCTTCGGTACAGATCATTGAt                     <  7:257219/139‑1 (MQ=255)                                                                                                                        tCATTTGCATGGATGCCAGCAGATTCATGCCCGGGCGCTGTGCCATGTTGCCGTAATGCCAATAGACCGCGCTTGGCGTACACTCCATTAAAAACAACGGCGGCTGCATCACGCTTCCGTACAGATCATTGATAAAGcc               >  6:412503/1‑139 (MQ=255)                                                                                                                            ttGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCAAGCTTCGGTACAGATCATTGATAAAGCCGAcc           >  6:88995/1‑139 (MQ=255)                                                                                                                                    aTGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATTCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCAAGCTTCGGTACAGATCATTGATAAAGCCGACCTTCAACGc   >  5:282307/1‑139 (MQ=255)                                                                                                                                    aTGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGgctgct                                                 >  1:30162/1‑93 (MQ=255)                                                                                                                                    aTGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGgctgct                                                 <  2:30162/93‑1 (MQ=255)                                                                                                                                        cagcagATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCAAGCTTCGGTACAGATCATTGATAAAGCCGACCTTCATCGcc  >  4:105802/1‑136 (MQ=255)                                                                                                                                          |                                                                                                                                      TTGAAAGACGCGGTTCTTCGGGCTATTGTCATGATCCACAACCGCTCCGTGTAATTTTTCTGATGACCCCCGTGATTTGCGGTATTGGAAATAGAGAACGCTGTCCGAGCCGTGGGCAATCATTTGCATGGATGACAGCAGATTCATGCCCGGGCGCTTTGCCTTGTTGACGTTATGCCAATTGACCGCGCTTGGCGTACACTCCATTAATAAGAAGGGCTGCTGCTTCAAGCTTCGGTACAGATCATTGATAAAGCCGACCTTCATCGCC  >  CP006881/3321768‑3322038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑