Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 389,037 T→G 20.0% A434A (GCT→GCG)  U712_01810 → Surfactin synthase subunit 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881389,0370TG20.0% 94.0 / 6.3 47A434A (GCT→GCG) U712_01810Surfactin synthase subunit 2
Reads supporting (aligned to +/- strand):  ref base T (6/31);  new base G (7/3);  total (13/34)
Fisher's exact test for biased strand distribution p-value = 2.13e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGTGTATCCGGGCAAAACGTGGACCGTCAAAATTAAATATAACGGAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCACCAAGTGTTTGAAGAGCAAGCGAAACGGACG  >  CP006881/388906‑389169
                                                                                                                                    |                                                                                                                                    
aTTGTGTATCCGGGCAAAACGTGGACCGTCAAAATTAAATATAACGGAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTtg                                                                                                                                  <  3:135703/136‑1 (MQ=255)
aTTGTGTATCCGGGCAAAACGTGGACCGTCAAAATTAAATATAACGGAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑GTTTGTGc                                                                                                                               <  1:268137/139‑1 (MQ=255)
aTTGTGTATCCGGGCAAAACGTGGACCGTCAAAATTAAATATAACGGAGCCGCCTTTGATTACGCTTTTATCGAACGGACAGCGGAGCACCGTACCCGCATGATGGAAGCAGCTGTCGATCCGCCGGCCGC‑GTTTGTGc                                                                                                                               >  5:159583/1‑139 (MQ=255)
   gtgtATCCGGGCAAAACGTGGACCGTCAAAATTAAATATAACGGAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTtgtg                                                                                                                                <  3:331093/135‑1 (MQ=255)
   gtgtATCCGGGCAAAACGTGGACCGTCAAAATAAAATATAACGGAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTtgtg                                                                                                                                <  4:132619/135‑1 (MQ=255)
          cGGGCAAAACGTGGACCGTCAAAATTAAATATAACGGAGCCGCCTTTGATTCCGCTTTTATAGAACGGACAGCGGAGCCCCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TGTTGTGCGTGAGTAAgg                                                                                                                     >  8:131830/1‑139 (MQ=255)
            tgCAAAACGTGGACCGCCAAAATTAAATAGAACGGAGCCGCCTTTGATACCGCTTTTAGCGAACGGACAGCGGAGCACCTTACCCGCATGCTGGAAGCAGCTGGCGATCAGCAGGCCGC‑TTCTGTGCGTGAGTACg                                                                                                                      <  4:318335/135‑1 (MQ=255)
            ggCAAAACGGGGACCGTCAAAATTAAATATAACGGAGCCGCCTTTGAATCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGc                                                                                                                   <  5:247289/139‑1 (MQ=255)
                   cGTGGACCGTCAAAATTAAATATAACGGAGACGCCTTTGATTCCGCTTTTATCGAACGGACAGCTGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTggg                                                                                                            <  1:241081/139‑1 (MQ=255)
                     tGGACCGTCAAAATTAAATATAACGGAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGgag                                                                                                          <  2:326180/139‑1 (MQ=255)
                        aCCGGCAAAATTAAATATAACGGAGCCGCCTTTGATTCCGCTTTAATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACg                                                                                                       <  7:371480/139‑1 (MQ=255)
                         ccGTAAAAATTAAATATAACGGAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACATTACCCGCATTATGTAAGTAGCTTTTGATAAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTgggtg                                                                                                          <  4:164968/135‑3 (MQ=255)
                                       tataACGGAGCCGCCTTTGAATCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGc                                                                                           <  4:193878/136‑1 (MQ=255)
                                             ggAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTg                                                                                     <  3:44204/136‑1 (MQ=255)
                                             ggAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCg                                                                                  <  8:55096/139‑1 (MQ=255)
                                             ggAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCg                                                                                  <  1:363411/139‑1 (MQ=255)
                                             ggAGCCGCCTTTGATTCCGATTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGACGCAGCTGTCGAGCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCg                                                                                  <  7:161577/139‑1 (MQ=255)
                                               aGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGCAGCAGCGGTCGCTCAGCCGGCCGC‑GTTTTTGCGTGGGTACGGGGGTGGTGGCGACGCAGTGCAGCCGCAAATTGTCGAg                                                                                >  6:170031/1‑139 (MQ=255)
                                               aGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAg                                                                                <  5:395198/139‑1 (MQ=255)
                                               aGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATAAGCCGGCCGCGTTTTGTGGGTGTGTACGGGGTTGGGGGGGCCGAAGCGCCCCGGCAAATTGTc                                                                                   >  2:261419/1‑137 (MQ=255)
                                               aGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGAGGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAg                                                                                <  1:161376/139‑1 (MQ=255)
                                               aGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCGTACCCGCATGATGGAAGCACCTGTCGATCAGCCGGCCGCGTTTTGGGGGTG‑GTACGGGCGTGTGGGGGACGGAGCGCAGAGGGCAATTGTGGAg                                                                                >  6:217924/1‑139 (MQ=255)
                                                  cgccTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑GTTTGTGCGTGAGTTCGGGCGTGTGGGAGCCGCAGAGCAGCGGCAAATTGTCgctgtg                                                                             >  8:270560/1‑134 (MQ=255)
                                                    ccTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑GTTTGTGGGGGGGTTCGGGGTTGTGGGTGGCGCAGAGGAGCCGCAAATTGTGGAGgt                                                                              >  3:404505/1‑136 (MQ=255)
                                                     agttGATTACGCTTTTATCGACCGGACAGCGGAGCACCTTATCCTGATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGGGttt                                                                          <  8:116183/137‑1 (MQ=255)
                                                         gATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAAc                                                                       <  1:158631/138‑1 (MQ=255)
                                                               gCTCTTCTCGAACGGACAGCGGAGCACCTAACCCGCATGCTGGAAGCAGCTGTCGATCAGCCGGCCGC‑GTTTGTGCGGGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAACAGCa                                                                   <  3:72951/136‑1 (MQ=255)
                                                                        gcaCGGACAGCGGAGCACCTTACGCGCATGATGGAAGCAGCTGGCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAACAGCACGAAAGcc                                                           <  4:213402/133‑1 (MQ=255)
                                                                        gaccGGACAGCGTAGCACCTTACCCGCATGATGGAAGGAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGTGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGaa                                                        <  1:322627/135‑1 (MQ=255)
                                                                        gAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGaa                                                        <  5:187040/138‑1 (MQ=255)
                                                                               cAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGG‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGa                                                <  7:51710/139‑1 (MQ=255)
                                                                                aGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGttt                                                                          >  2:350069/1‑112 (MQ=255)
                                                                                aGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGttt                                                                          <  1:350069/112‑1 (MQ=255)
                                                                                        aCCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAAt                                          <  4:192542/136‑1 (MQ=255)
                                                                                          cTTACCCGCATTATGGAAGGAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGGTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATgg                                        <  3:393580/136‑1 (MQ=255)
                                                                                          cTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTTCGTGAGTACGGGCGTGTGGGAGACCAAGAGCAGCGGCAAATTGTGGAGGTGTTTTAAAGCACGAAAGCCGCACTCCCTGAAGGAATgg                                        >  4:305279/1‑136 (MQ=255)
                                                                                                 gcctgatgGAAGCAGCGGTCGAGCAGCCGGCCGC‑GTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCACCa                              <  6:169957/136‑1 (MQ=255)
                                                                                                 gCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCa                                 <  4:267605/136‑1 (MQ=255)
                                                                                                 gCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCACCa                              <  1:261419/139‑1 (MQ=255)
                                                                                                 gCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGGGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCACCa                              <  1:28868/139‑1 (MQ=255)
                                                                                                    tgatgGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCACCAAgt                           <  6:159583/139‑1 (MQ=255)
                                                                                                      atgGAAGCAGCTGTCGATCAGCCGGCCGC‑GTTTTTGGGTGGGTTAGGGCGTGTGGGAGACGCAGAGCCGCGGCAAATTGTGGAGGTGTTTAACAACACGAAACCCGCACTCCCGGAAGGAAAGGCTGTTcaacaaa                            >  4:89224/1‑135 (MQ=255)
                                                                                                           agcagcTGTCGATCAGCCGGCCGC‑GTTTTTGGGTGAGTAAGGGCTTTTTGGATCCGAAGAGCACCCGCAAAATTTAGTGGTGTTTAAAATCACGAAATACCAACACCCGGAATGAAAGGCTGTTCAACAACTGTTTgtt                    >  2:353280/1‑137 (MQ=255)
                                                                                                               gcTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCACCAAGTGTTTGAAGAGc                <  7:131830/139‑1 (MQ=255)
                                                                                                                     gATCAGCCGGCCGC‑TTTTGTGCGTGAGTTAGGGGGTGTGGGAGCCGAAGAGCAGCGGCAAATTGTCGAGGTGGTTTACAGCACGAAAGCCGCACGCCCTGAAGGAATGGCTGTTCACCAAGTGTTTGAAGAGCAAGCg           >  7:348449/1‑138 (MQ=255)
                                                                                                                             ggccgC‑TTTTGTGCGGGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCACCAAGTGTTTGAAGAGCAAGCGAAACGGACg  <  8:349541/139‑1 (MQ=255)
                                                                                                                             ggccgC‑GTTTGTGCGTGTGTTCGGGCGTGTGGGAGACGAAGAGCACCGGCAAAATTTGGAGGTGTTTAAAAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCACCAAGTGTTTGTAGAGCAAGCGAAACCGaag  >  2:66928/1‑137 (MQ=255)
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ATTGTGTATCCGGGCAAAACGTGGACCGTCAAAATTAAATATAACGGAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGC‑TTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGCGGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCACCAAGTGTTTGAAGAGCAAGCGAAACGGACG  >  CP006881/388906‑389169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑