| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,615,482 | C→A | 28.5% | E177* (GAA→TAA) | U712_18635 ← | Peptide chain release factor 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,615,482 | 0 | C | A | 28.5% | 19.9 / 3.8 | 31 | E177* (GAA→TAA) | U712_18635 | Peptide chain release factor 1 |
| Reads supporting (aligned to +/- strand): ref base C (8/10); new base A (2/7); total (14/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATCCTTCTCATGGATGTCGACCTCTACCTCTTCTGCCTCAGGAAGACATGCCACTGTTGCAGTGGATGTATGGATGCGTCCGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTCATATTTGAGTTTAGAATACGCGCCGCTTCCTGTAATCATAAAGATGATCTCTTTGTAGCCGCCCGTTCCGGTTACATTCGCTTCCATGACCTCTGTTTTCCAGCCCTGAAGCTCAGCATAACGGGAATACA > CP006881/3615353‑3615613 | tatCCTTCTCATGGATGTCGACCTCTACCTCTTCTGCCTCAGGAAGACATGCCACTGTTGCAGTGGATGTATGGATGCGTCCGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTGTTGATAttt > 3:159248/1‑136 (MQ=255) tCCTTCTCATGGATGTCGACCTCTACCTCTTCTGCCTCAGGAAGACATGCCACTGTTGCAGTGGATGTATGGATGCGTCCGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTCATATTTGAGt < 7:69472/138‑1 (MQ=255) tCGACCTCAACCTCTTCTGCCTCTGGAAGACATGCCAGTGTTGCTGTGGATGTATGGAGGCGTCCGCCTGTTTCTGTTTCCGGAACACGCTGTAGGCGGTGGGCGCCGTTTTAATATTTTAGTTTAGAATAcgcg < 4:159248/135‑1 (MQ=255) cctctTCTGCCTCCGGAAGACATGCCACTGTTGCTGTGGATGTATGGAGGCGGCCGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTCATATTTGAGTTTATAATACGCGCCGCTTCCTGt < 6:90600/136‑1 (MQ=255) cctctTCTGCCTCAGGAAGACATGCCACTGTTGCAGTGGATGTATGGATGCGTCCGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTCATATTTGAGTTTAGAATACGCGCCGCTTCCTGt < 5:176598/136‑1 (MQ=255) tctGCCTCAGGAAGACATGCCACTGTTGCAGTGGATGTATGGATGCGTCCGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTCATAttt > 8:49822/1‑104 (MQ=255) tctGCCTCAGGAAGACATGCCACTGTTGCAGTGGATGTATGGATGCGTCCGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTCATAttt < 7:49822/104‑1 (MQ=255) gCCACTGTTGCAGTGGATGTATGGATGCGTACGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTCATATTTGAGTTTAGAATACGCGCCGCTTCCTGTAATCATAAAgatgat < 6:392262/128‑1 (MQ=255) gCCACTGTTGCAGTGGATGTATGGATGCGTACGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTCATATTTGAGTTTAGAATACGCGCCGCTTCCTGTAATCATAAAgatgat > 5:392262/1‑128 (MQ=255) gCAGTGGATGTTTGGATGCGTCGGCATGATTATGTTTCCGGAAGAGCCTTTACGGGGGGGGCGCATTTTTAATATTTGAGTTTACAATACGCGCCGCTTCCTGTAATCATAAAGATGATCTCTTTGTAGCCGCCCGTTc < 7:79402/139‑1 (MQ=255) gTGGATGTATGGATGCGTCCGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTGATATTTGAGTTTTTAATACGCGCCGCTGACTGTAATAATAAAGATGATCTCTTTGTAGCCGCCCGGTCCgg > 6:238808/1‑139 (MQ=255) gTGGATGTATGGATGCGTCCGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTGATATTTGAGTTTAGAATACGCGCCCCGTCCTGTAATAATAAAGATGGTGTCTTTGTAGCCGCCCGGTCCgg > 5:95909/1‑139 (MQ=255) gTGGATGTATGGATGCGTCCGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTCATATTTGAGTTTTGAAAACGCGCCCCGTCCTGTAATCATAAAGATTATATATTTGTAGCCGCCCGGTCCgg > 8:79402/1‑139 (MQ=255) tGTATGGATGCGTCCGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTGTTCATTTTTGAGTTTAGAATACGCCGCGCGTCCTTTTATAATAAATATTTTTAAGTTTTTGCCGCccggtccggtagca > 5:426734/1‑135 (MQ=255) ggttGCGGCCGCCTTATTCTGTTTCCGGACCAGGGTGTCCGGGGGGGGCGCGGTTTTATTATTTGAGTTAAGAATCCGCGCCGCTTCCTGTAATCATAAAGATGATCTCTTTGTAGCCGCCCGTTCCGGTTACAt < 3:277431/132‑1 (MQ=255) ggATGCGGCCGCCTGATTCGGTTTCCGCAACACGCTGTACGCGGTGGGCGCCGTTTTCATATTGGAGTTTAGAATCCGCGCCGCTTCCTGTAATCATAAAGATGATCTCTTTGTAGCCGCCCGTTCCGGTTACATTCg < 1:49179/138‑1 (MQ=255) tGCGTCCGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTAATATTTGAGTTTAGAATACGCGCCGCTTCCTGTTATCATAAAGATGATCGATTTGTAGCCGCCCGTTGCGGTTTCCTTCGcgct > 1:57840/1‑136 (MQ=255) gTCCGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTCATATTTGAGTTTTGAATACGCGCCGCGTCCTGTTATCATAAAGATGATCTGTTTTTTGCCGCCCGGTCCGGGTACAATCGGTTGCAt > 5:89815/1‑139 (MQ=255) tCCGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTGATATTTGAGTTTATAATAAGCGCCGCGGACTGTAATCATAAATATTATCTCTTTGTTTCCCCCCGTTCCGGTTTCATTAGCTTGCATg > 5:15758/1‑139 (MQ=255) ggcgtGATTGTGTTTCCGCACCCGGCTGTCCGGGGGGGGCGCGTTTTTAATTTTTGTTTTTAGAATCCGCGCCGCTTCCTGTAATCATAAAGATGATCTCTTTGTAGCCGCCCGTTCCGGTTACATTCGCTTCCATGAc < 1:423190/135‑1 (MQ=255) cGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTCATTTTTGAGTTTAGAATACGCGCCGCTTCCTGTAATCATAAAgatgat > 6:202083/1‑97 (MQ=255) cGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTCATATTTGAGTTTAGAATACGCGCCGCTTCCTGTAATCATAAAgatgat < 5:202083/97‑1 (MQ=255) tGTTTCCGGAACACGCTGTCCGCGGTGGGCGCCGTTTTAATATTTGAGTTTAGAATACGCGCCGCTTCCTGTAATCATAAAGATGATCTCTTTGTAGCCGCCCGTTCCGGTTACATTCGCTTCCATGACCTCTGtt < 3:398161/136‑1 (MQ=255) gAACAGGCTGTACGCGGTGGGCGACTTTTTCATATTTGAGTTTAGAATACGCGCCGCTTCCTGTAATCATAAAGATGATCTCTTTGTAGCCGCCCGTTCCGGTTACATTCGCTTCCATGACCTCTGTTTTCCAGCCCTg < 6:143848/139‑1 (MQ=255) tACGCGGTGGGCGCCGTTTTAATATTTGAGTTTAGAATACGCGCCGCTTCCTGTAATCATAAAGATGATCTCTGTTTAGCCGCCCGGTCCGGGTACATTCGCGTCCATGACCTCTGTTTTGCAGCCCTGAAGCGGAGCa > 1:141894/1‑139 (MQ=255) ggTGGGCGCCGTTTTCATATTTGAGTTTAGAATACGCGCCGCTTCCTGTAATCATAAAGATGATCTCTTTGTAGCCGCCCGTTCCGGTTACATTCGCTTCCATGACCTCTGTTTTCCAGCCCTGAAGCTCAGCATAACg < 6:95909/139‑1 (MQ=255) gggCGCCGTTTTCATATTTGAGTTTAGAATACGCGCCGCTTCCTGTAATCATAAAGATGATCTCTTTGTAGCCGCCCGTTCCGGTTACATTCGCTTCCATGACCTCTGTTTTCCAGCCCTGAAGCTCAGCATAACggg > 6:406863/1‑138 (MQ=255) gcgcTGTTTTAATATTTGAGTTAAGAATACGAGCCGCTTCCTGTAATCATAAAGTTGATCTCTTTGTAGCCGCCCGTTCCGGTTACATTCGCTTCCATGACCTCTGTTTTCCAGCCCTGAAGCTCAGCATAACGGGAAt < 6:426734/139‑1 (MQ=255) gcgcGGTTTTAATTTTTTAGTTTATAAACCGCGCCGCTTCCTGTAATCATAAAGATGATCTCTTTGTAGCCGCCCGTTCCGGTTACATTCGCTTCCATGACCTCTGTTTTCCAGCCCTGAAGCTCAGCATAACGGGAAt < 6:15758/139‑1 (MQ=255) gcgcCGTTTTCATATTTGAGTTTAGAATACGCGCCGCTTCCTGTAATCATAAAGATGATCTCTTTGTAGCCGCCCGTTCCGGTTACATTCGCTTCCATGACCTCTGTTTTCCAGCCCTGAAGCTCAGCATAACGGGaa > 5:360651/1‑138 (MQ=255) ccGTTTTCATATTTGAGTTTAGAATACGCGCCGCTTCCTGTAATCATAAAGATGATCTCTTTGTAGCCGCCCGTTCCGGTTACATTCGCTTCCATGACCTCTGTTTTCCAGCCCTGAAGCTCAGCATAACGGGAATACa < 6:89815/139‑1 (MQ=255) | TATCCTTCTCATGGATGTCGACCTCTACCTCTTCTGCCTCAGGAAGACATGCCACTGTTGCAGTGGATGTATGGATGCGTCCGCCTGATTCTGTTTCCGGAACACGCTGTACGCGGTGGGCGCCGTTTTCATATTTGAGTTTAGAATACGCGCCGCTTCCTGTAATCATAAAGATGATCTCTTTGTAGCCGCCCGTTCCGGTTACATTCGCTTCCATGACCTCTGTTTTCCAGCCCTGAAGCTCAGCATAACGGGAATACA > CP006881/3615353‑3615613 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |