Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,725,209	+T	intergenic (‑41/‑488)	U712_19205 ← / → U712_19210	Uncharacterized protein ywcI/Minor extracellular protease vpr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,205	1	.	T	82.9%	200.3 / 38.3	78	intergenic (‑37/‑492)	U712_19205/U712_19210	Uncharacterized protein ywcI/Minor extracellular protease vpr
Reads supporting (aligned to +/- strand):  ref base . (2/11);  new base T (42/21);  total (46/32)
Fisher's exact test for biased strand distribution p-value = 1.21e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑C‑T‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTT  >  CP006881/3725068‑3725326                                                                                                                                              |                                                                                                                               tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCCGGCA‑GC‑C‑t                                                                                                                               >  7:322853/1‑139 (MQ=255) tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCAGCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑C‑t                                                                                                                               >  4:408238/1‑139 (MQ=255)  gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTGTTTTTGGCAGGCA‑GC‑C‑t‑t                                                                                                                             >  7:29279/1‑139 (MQ=255)  gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑C‑t‑t                                                                                                                             >  4:104020/1‑139 (MQ=255)  gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑C‑t‑t                                                                                                                             >  5:130744/1‑139 (MQ=255)        aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑C‑t‑ttttag                                                                                                                        >  1:409288/1‑135 (MQ=255)             accgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGccc                                                                                                                 >  8:381092/4‑139 (MQ=255)             accgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGccc                                                                                                                 <  3:104020/136‑1 (MQ=255)             accgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGccc                                                                                                                 >  4:387060/4‑139 (MQ=255)             accgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTGCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGccc                                                                                                                 >  8:337554/4‑139 (MQ=255)               cgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑t                                                                                                              >  7:486636/2‑139 (MQ=255)               cgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGGAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCATCTTTATGGCATGCC‑GC‑CGT‑TTT‑A‑GCAGCCCG‑t                                                                                                              >  3:100202/2‑139 (MQ=255)                  ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑T‑GCAGCCCG‑tgtt                                                                                                           >  6:132830/1‑139 (MQ=255)                  ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑tttt                                                                                                           >  6:235015/1‑139 (MQ=255)                  ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑tttt                                                                                                           >  7:26536/1‑139 (MQ=255)                  ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑tttt                                                                                                           <  7:363299/139‑1 (MQ=255)                  ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑ttt                                                                                                            >  8:194278/1‑138 (MQ=255)                    cAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTt‑ct                                                                                                        <  5:182843/139‑1 (MQ=255)                    cAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTt‑ct                                                                                                        <  4:78960/139‑1 (MQ=255)                          cAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGTCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCg                                                                                                  <  2:409288/139‑1 (MQ=255)                          cAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCg                                                                                                  <  8:486636/139‑1 (MQ=255)                                 tCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGccc                                                                                            <  7:194278/138‑1 (MQ=255)                                 tCTGTCCGGCGTCACCCACTTCATCAAAAAGACCATCCACACCCGTTAAGATACAAGCAAACG‑TTTCATAGAAAAACAGCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑C‑TGTTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGcccg                                                                                           >  5:230427/1‑138 (MQ=255)                                     tCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGcccgc                                                                                          >  3:193666/1‑134 (MQ=255)                                       cGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGcccgcaaga                                                                                      >  1:252293/1‑132 (MQ=255)                                                    ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACCACCCTCCGCTTCTTTTTGGCAGGCAGGC‑A‑T‑TTT‑T‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCCCAAGACAAGCCGCCCGAtt                                                                        >  4:461684/1‑139 (MQ=255)                                                    ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtt                                                                        >  5:236814/1‑139 (MQ=255)                                                    ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtt                                                                        >  4:117194/1‑139 (MQ=255)                                                         caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTTGCAGGCA‑GC‑C‑t‑ttttagc                                                                                                                       >  2:269016/1‑86 (MQ=255)                                                         caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttcttt                                                                   >  6:400377/1‑139 (MQ=255)                                                         caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttcttt                                                                   >  1:396239/1‑139 (MQ=255)                                                         caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑C‑t‑ttttagc                                                                                                                       <  1:269016/90‑5 (MQ=255)                                                         caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCGTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAgttcgtt                                                                   >  1:402764/1‑139 (MQ=255)                                                         caAAAACACCACCCACACCCGTAAACATACAAGCAAACG‑TTACATAGAAAAACACCCCTCCGCTTCTTTTTGGCCGGCA‑GC‑CTT‑TTT‑C‑CCAGCCCG‑TTTT‑CTCCCCCCCCCCCCGCAACAAAAGACGGCCGAtttcttt                                                                   <  4:82895/139‑1 (MQ=255)                                                              aGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCg                                                              >  3:206701/1‑139 (MQ=255)                                                              aGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCg                                                              >  2:268793/1‑139 (MQ=255)                                                                accaaccaCCCCCTTAAATAAACCAGCAACCG‑TTACATAGAAACACATCCCTCCGATTATTTTTGGCAGGCC‑GCAT‑T‑TTT‑A‑GCCGCCGT‑TTTT‑CTCCCCCGCACCCCTCAAAAAACGACGGCCGATTTCTTTTCTCGCa                                                            <  4:492794/139‑1 (MQ=255)                                                                   aTCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCGCGCAAAc                                                         >  7:2352/1‑139 (MQ=255)                                                                    tCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTATCGCAAAc                                                         >  5:403082/1‑138 (MQ=255)                                                                       acacCCTTAAAGAAACAAGCAACCG‑TTTAATACAAAAACATCCCTCCGTTTTTTTTTGCCCGCCA‑GCTT‑T‑TTT‑A‑CCAGCCCG‑TTTT‑CACCCCCCCCGCCCCAAACAAACGACGGCCGATTTCTTTTCTCGCAAACTAGc                                                     <  4:193666/139‑1 (MQ=255)                                                                         acCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCCCAAGAAAAGACGGCCGATTTCATTTATCGAAAACTATCgg                                                   >  5:122788/1‑139 (MQ=255)                                                                          cccGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCATCCGCAGCCCGCAAGATAAGACGGCCGATTTCTTTTCTCg                                                              >  8:262046/1‑127 (MQ=255)                                                                          cccGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGATAAGACGGCCGATTTCTTTTCTCg                                                              <  7:262046/127‑1 (MQ=255)                                                                              tAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGGA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCGTTTCTCGCAAAAGAGCGGcgg                                                >  4:299613/1‑135 (MQ=255)                                                                              tAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCC‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAATTATTTTCTCGCAAACTAGCGGcgc                                                >  1:343922/1‑135 (MQ=255)                                                                              tAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGccc                                                                                                                 >  2:335244/1‑74 (MQ=255)                                                                              tAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGccc                                                                                                                 <  1:335244/74‑1 (MQ=255)                                                                              tAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGcc                                                                                             >  7:163411/1‑92 (MQ=255)                                                                              tAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGcc                                                                                             <  8:163411/92‑1 (MQ=255)                                                                              tAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc                                                >  4:316512/1‑137 (MQ=255)                                                                              tAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc                                                >  7:336418/1‑137 (MQ=255)                                                                              tAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTa                                              >  4:489286/1‑139 (MQ=255)                                                                              tAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CGT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc                                                >  5:94140/1‑137 (MQ=255)                                                                                 acaaaCAAGCAAACG‑TTCAAAACAAAAACAACCCCCCGCTTCTTTTTGGCCGCCC‑GC‑CTT‑TTT‑C‑GCCGCCGG‑TTTG‑CTCCGCCCCCCCCCGAAAAAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa                                           <  5:132830/135‑1 (MQ=255)                                                                                 acaTACAACCAAACG‑TTTCATATAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCCGCCCG‑TTTT‑CTCAGCCGCCGCCCGAAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa                                           <  5:123473/137‑1 (MQ=255)                                                                                     aCAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCC‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaaa                                        >  3:303367/1‑138 (MQ=255)                                                                                     aCAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCCGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc                                       >  6:197691/1‑139 (MQ=255)                                                                                     aCAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCCGCCCTCAAGAAAATACGGCCGAGTTGTTTTATAGGACACAAGCGGCAAGAGCAAAAc                                       >  1:435477/1‑139 (MQ=255)                                                                                     aCAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc                                       >  5:239357/1‑139 (MQ=255)                                                                                     aCAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCGAAAGCAAAAc                                       >  5:266809/1‑139 (MQ=255)                                                                                     aCAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTATCGCAAACTAGCGGCTCTAGCaaaa                                        >  1:259418/1‑138 (MQ=255)                                                                                     aCAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTATTTTCTCGCAAACTAGCGGCTCTAGCAAAAc                                       >  6:123473/1‑139 (MQ=255)                                                                                     aCAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCGTTTCTCGCAAACTAGCGGCTCGAGCAAAAc                                       >  7:418780/1‑139 (MQ=255)                                                                                     aCAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGAA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCCCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaaa                                        >  7:294400/1‑138 (MQ=255)                                                                                     aCAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCATTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGCCTGCCGATTTCTTTTCACGCAAACTAGCGGCTCGAGGAAAAc                                       >  1:383912/1‑139 (MQ=255)                                                                                         caacaCG‑TTTCCTCGAAAAAACCCCCTCCCCTTCTTTTGGGCCGGCA‑GC‑CTT‑TTT‑A‑GCCGCCCG‑TTTA‑CCCCGCCGCCGCCCCCAATAAACCCCGGCCGATTTCTTTGCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                   <  6:130744/135‑1 (MQ=255)                                                                                         caaaaCG‑TTTCAAAGAAAAACAACCCTCCGCTTCTTTTTGCCCGGCA‑CC‑CTT‑TTT‑A‑GCCGCCCG‑TTTT‑CTCCCCCCCAGCCCGCAACAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                   <  7:474695/137‑1 (MQ=255)                                                                                          cAAACGTTTTAATATAAAAACACCCCGCGGTTTTTTTTGGGCAGGCC‑CC‑CTT‑TTT‑A‑GCCGCCGG‑TTTG‑CCCCCCCCCCGCCCCAAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                   <  4:206701/139‑1 (MQ=255)                                                                                           aaaCT‑TTTCTAACAAACACAGCCCTCCGCTTCTTTTGGGCAGCCA‑GC‑C‑T‑TTT‑ACGCACCCCG‑TATC‑CTCCGCCGCAGCCCGCAACAAAACACGGCCGTTTTCTTTTCTGGCAAACTAGCGGCTCTAGCAAAACTCATGa                                 <  6:198500/139‑1 (MQ=255)                                                                                           aaaCG‑TTTAATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑C‑T‑TTT‑ACGCAGCCCG‑TTTT‑CTCAGCCCCCGCCCGCAAAAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa                                 <  1:268793/139‑1 (MQ=255)                                                                                             gcG‑TTTCATACAAAACCAGCCCGCGGTTTGTTTTTGGCGGCCC‑GCGT‑T‑TTT‑T‑CCCCCCCG‑TTTT‑CTCCGCCCCCCCCCGAAACAAATCCCGGCCGATTTCTTTTCTCGCAAACAAGCGGCTCTAGCAAAACTCATGAct                               <  2:343922/138‑1 (MQ=255)                                                                                             aCG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                               <  3:489286/139‑1 (MQ=255)                                                                                             aCG‑TTTCATACAAAACCACCCCTCCGGTTCTGTTGGGACGGCC‑GC‑C‑T‑TTT‑ACCCCGCCCG‑TTTC‑CTCTCCCCCCGCCCGCAACAAACGACGGCCGATGTCTTTTCTCGCAACCTAGCGGCTCTAGCAAAACTCATGAct                               <  6:94140/139‑1 (MQ=255)                                                                                             aCG‑TTTCAAACAAAAACACCCCTCCGCTTATTTTTGGCCGCCC‑GC‑CTT‑TTT‑A‑GCA‑CCCG‑TTTTGCCCCGCCGCACCCCCAAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                               <  5:235015/139‑1 (MQ=255)                                                                                             aCG‑TTTAATAGAAAAACATCCCGCCGCTTGTTTTGGCCCGGCA‑GCTT‑T‑TTT‑C‑CCACCCCG‑TGTC‑CGACGCCCAACCCCCCAAGAAACCACGGCCGATTTCTTTTCTCGCAAACAAGCGGCTCTAGCAAAACTCATGAct                               <  8:29279/139‑1 (MQ=255)                                                                                             aCG‑TTTAATACAAAAACATCCCTCGGCTTATTTTTGGCCGGCC‑GCGT‑T‑TTT‑A‑GCCGCCCG‑TTTT‑CCCCGCCCCCCCCCCCAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                               <  7:381092/139‑1 (MQ=255)                                                                                             aCG‑ATTAATATAAAAACATCCCTCCGATTCTTTTTGGCCGCCA‑GC‑CTT‑TTT‑A‑CCCGCCCG‑TTTT‑CCCAGCCGCAGCCCCCAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                               <  2:252293/139‑1 (MQ=255)                                                                                              actttacATACAAAAACATCCCTCCGCTTCTTTTTGCCCGGCA‑GC‑CTT‑TTT‑C‑GCCCCCCG‑TTTT‑CTCAGCCCCAGCCCGAAACAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                               <  3:387060/133‑1 (MQ=255)                                                                                                     acacctaaaCATCCCTCGGATTTTTTTTGGAAGGCA‑GC‑CTT‑TTT‑C‑GCAGCCGG‑TTTA‑CTCAGCGGCCCCCCGCAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGAt                        <  6:468488/133‑1 (MQ=255)                                                                                                        caaaaaCACCCCCCCGCTCTTTTTTGGCCGGCC‑GC‑T‑T‑TTT‑T‑ACCCCCCGTTTTT‑CACCGCCCCCCCCCCCAACAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGa                     <  5:400377/138‑1 (MQ=255)                                                                                                          aaaaCATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGAcc                   >  2:40414/1‑139 (MQ=255)                                                                                                              cATCCCTCCGTTTCTTTTTGCCCGTCT‑GCTT‑T‑TTT‑C‑GCCGCCCT‑TTTT‑CTCGGCCGCAGCCCCAAAAAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTgg               <  1:151304/139‑1 (MQ=255)                                                                                                                 cccTCCGCTTCTTTTTGGCAGGCA‑GC‑C‑T‑TTTAA‑GCCGCCGG‑TTTT‑CACAGCCGCACCCCCCAATAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGaaa            <  7:16261/139‑1 (MQ=255)                                                                                                                 cccTCCGCGTCTTTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTCTCGCAAACGAGCCGCTCGATGAAAACTCATGCCTCTATGATACTCCGTggg              >  3:4134/1‑136 (MQ=255)                                                                                                                        cttcttTTTTGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCGCAAGAAAACACGGCCGATTTATTTTATCGCAAACTAGCGGCTCTATCAAAACAAATGACTATATGAAATACCGTGGAAAGAGAAt      >  2:151304/1‑138 (MQ=255)                                                                                                                           cttTTTGGCAGGCA‑GC‑CTT‑TTT‑A‑GCAGCCCG‑TTTT‑CTCACCCGCAGCCCGCAAGAAAAGACGGCCGAATTCTTTTATCCCAAAATAGCGCCTCTAGCAAAACACAAGACTAAATGATAGACACTCGAAAGaaaaataat  >  8:410023/1‑134 (MQ=255)                                                                                                                                              |                                                                                                                               TGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACG‑TTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑C‑T‑TTT‑A‑GCAGCCCG‑TTTT‑CTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTT  >  CP006881/3725068‑3725326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑