Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,725,031	C→T	A46A (GCG→GCA)	U712_19205 ←	Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,031	0	C	T	94.9%	126.0 / ‑6.4	39	A46A (GCG→GCA)	U712_19205	Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (7/30);  minor base A (2/0);  total (9/30)
Fisher's exact test for biased strand distribution p-value = 4.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACA  >  CP006881/3724896‑3725154                                                                                                                                        |                                                                                                                            cGGGCCCGTTCTCTACTGCTTTGGCGATTCCTTGATGGCTTGCAAAAATCTTATTTAAAAACGCGGGGCGGCTTTCGCTTTCTGCTTTTTTTCCTCATTCACAGCCCTCCGCCATTCATAAAACGAAACGAGTCCtgcg                                                                                                                          <  2:385429/139‑1 (MQ=255) cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTGTGGGTTTATTACTCATTCACACCCCTCCGCCATGCATAAAACGCAACCAGTCCtgcg                                                                                                                          >  8:16261/1‑139 (MQ=255)   ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTGACAGCCCTCCGCCCTTCATAAAACGAAACGAGTCCTGCGGc                                                                                                                        >  3:217378/1‑139 (MQ=255)              tcCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCGTTAGCTTTATTGTTTTATTTCTCATTAACACCCAACCGCCCATAATAAAAACAAACCAGTCCAGCGGGCGGCAcccccg                                                                                                             >  1:445763/1‑139 (MQ=255)                cTGGTTTGGCGATTCCTGGAAGGATTGCAAACATCTTCTTTTATAATCCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCTTTCATAAAACGAAACGAGTCCTGCGGCCTGCAcagccg                                                                                                             <  6:45652/137‑1 (MQ=255)                   cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                           >  7:42390/1‑135 (MQ=255)                   cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                           >  7:369175/1‑135 (MQ=255)                   cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                           <  8:42390/136‑2 (MQ=255)                   cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                           <  8:369175/136‑2 (MQ=255)                   cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCGTTAGCGTTATGCTTTTAATCCTAATTAACAGCCATACGCCCATCAAAAAACCAAACCACTCCAGGGGCCGGCACcgccgcccgg                                                                                                        >  7:189654/1‑135 (MQ=255)                      tGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt                                                                                                      <  7:361783/138‑1 (MQ=255)                        gCGATTCCTTGAAGGCTTGCAAACTTCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCAATTCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                    <  1:223735/138‑1 (MQ=255)                        gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                    <  7:63801/138‑1 (MQ=255)                        gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTAttt                                                                                                    <  8:59887/138‑1 (MQ=255)                            ttCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTAt                                                                                               <  8:386819/139‑1 (MQ=255)                                  gAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg                                                                                          <  7:412341/138‑1 (MQ=255)                                  gAAGGCTTGAAAACATCTTCTTTTATACCGCGAGGGGGCTTTCGCTTTCTGATTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg                                                                                          <  7:313948/138‑1 (MQ=255)                                  gAAGGCTTGAAAACATCTTATTTTAAAACGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTAATCCACCGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg                                                                                          <  6:321400/138‑1 (MQ=255)                                  gAAGGCTGGCAAACTTCTTCTTTTATAACGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg                                                                                          <  2:416086/138‑1 (MQ=255)                                   aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                        <  6:242918/139‑1 (MQ=255)                                   aaGGCTTGCAAAAATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                        <  5:451227/139‑1 (MQ=255)                                      gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                     <  6:139717/139‑1 (MQ=255)                                      gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                     <  8:411670/139‑1 (MQ=255)                                      gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                     <  5:333356/139‑1 (MQ=255)                                      gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                     <  3:493000/139‑1 (MQ=255)                                      gCTTGCAAACATCTGCTTTTATAAAGCGCGGCGGCTTGCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCAATACATAAACCCAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                     <  3:252210/139‑1 (MQ=255)                                         tGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCtgcg                                                                                                                          <  8:472524/99‑1 (MQ=255)                                         tGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCtgcg                                                                                                                          >  7:472524/1‑99 (MQ=255)                                                          atcaaGCGCGGCGGCTTTCGCTTTCTGCTTTACTTCCTCATTCACCGCCTTCCGCAAATCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                  <  2:352189/135‑1 (MQ=255)                                                          ataACGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACCGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                  <  1:209971/138‑1 (MQ=255)                                                          ataAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                  <  3:463070/138‑1 (MQ=255)                                                          ataAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                  <  2:445763/138‑1 (MQ=255)                                                                        cTTTCGCTTTCTGCGTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                   <  8:189654/139‑1 (MQ=255)                                                                        cTTTCGATTTCTGCTTTTCTTCATCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGATCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                   <  5:391881/139‑1 (MQ=255)                                                                                   tgcttttCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                <  7:411998/112‑1 (MQ=255)                                                                                   tgcttttCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                >  8:411998/4‑115 (MQ=255)                                                                                                 aTTCCCAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCaaaa                            <  1:161893/137‑1 (MQ=255)                                                                                                         ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                  <  6:62940/139‑1 (MQ=255)                                                                                                            tCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCTGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                   >  6:431220/1‑103 (MQ=255)                                                                                                            tCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCTGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                   <  5:431220/103‑1 (MQ=255)                                                                                                            tCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTGTTTGGTTTGTACGCTGAGCCGAATAGACCCCCGCCGCCGCCGGTTTTATGTGCGGCCTCCCCCCGTTCATCAAAAAGCCCATCCacac               >  8:302870/1‑139 (MQ=255)                                                                                                              cGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACAccc             <  7:302870/139‑1 (MQ=255)                                                                                                                          aacgaaacgaGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCCCCCAGTTCCTCAAAAAGACCCTCCACACCCCGAAAGATACa  >  8:363299/1‑138 (MQ=255)                                                                                                                                        |                                                                                                                            CGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACA  >  CP006881/3724896‑3725154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑