Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,725,082	A→C	A29A (GCT→GCG)	U712_19205 ←	Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,082	0	A	C	100.0%	135.5 / NA	38	A29A (GCT→GCG)	U712_19205	Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (16/22);  total (16/22)

ATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTAGC  >  CP006881/3724953‑3725212                                                                                                                                  |                                                                                                                                   atcaaGCGCGGCGGCTTTCGCTTTCTGCTTTACTTCCTCATTCACCGCCTTCCGCAAATCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                                                                            <  2:352189/135‑1 (MQ=255) ataACGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACCGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                                                                            <  1:209971/138‑1 (MQ=255) ataAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                                                                            <  2:445763/138‑1 (MQ=255) ataAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                                                                            <  3:463070/138‑1 (MQ=255)               cTTTCGCTTTCTGCGTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                                                             <  8:189654/139‑1 (MQ=255)               cTTTCGATTTCTGCTTTTCTTCATCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGATCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                                                             <  5:391881/139‑1 (MQ=255)                          tgcttttCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                                                                          >  8:411998/4‑115 (MQ=255)                          tgcttttCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                                                                          <  7:411998/112‑1 (MQ=255)                                        aTTCCCAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCaaaa                                                                                      <  1:161893/137‑1 (MQ=255)                                                ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                            <  6:62940/139‑1 (MQ=255)                                                   tCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCTGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                                                             <  5:431220/103‑1 (MQ=255)                                                   tCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCTGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                                                             >  6:431220/1‑103 (MQ=255)                                                   tCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTGTTTGGTTTGTACGCTGAGCCGAATAGACCCCCGCCGCCGCCGGTTTTATGTGCGGCCTCCCCCCGTTCATCAAAAAGCCCATCCacac                                                                         >  8:302870/1‑139 (MQ=255)                                                     cGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACAccc                                                                       <  7:302870/139‑1 (MQ=255)                                                                 aacgaaacgaGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCCCCCAGTTCCTCAAAAAGACCCTCCACACCCCGAAAGATACa                                                            >  8:363299/1‑138 (MQ=255)                                                                              ctgctgCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCTTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCAt                                              <  7:138362/134‑1 (MQ=255)                                                                                      tGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                            <  4:493945/101‑1 (MQ=255)                                                                                      tGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                            >  3:493945/1‑101 (MQ=255)                                                                                        cacaGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACAt                                    <  4:433710/139‑1 (MQ=255)                                                                                             ccgcCTTTCTTTGGTTTGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTATGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCCGAAAGATACCAGCAAACGTTTCATATAAAAAAATCCCTc                               >  8:212468/1‑139 (MQ=255)                                                                                                  tttCTTTGGTATGTACGCTGCGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTGCGGCGTCACCCAGTTGATCAAAAAGACCATCCCCACCCGTAAAGATACAAGCAAACGTTTAATAGAACAACCTCCCTCCGcgt                          >  2:280277/1‑137 (MQ=255)                                                                                                  gttCTTTGGTATGTACGCTGCGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCCCCCCCGGAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGctt                          >  8:474695/2‑139 (MQ=255)                                                                                                               tACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCCCCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggc             >  7:17034/1‑139 (MQ=255)                                                                                                               tACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggc             <  2:264879/139‑1 (MQ=255)                                                                                                                 cGCTGAGCCGAATCGACCGCGGCAGCCGCAGGTGTTCTGGCCGGCGTCACCCAGTTCATCAACAAGACCATCCACACCCGTACAGAGACAAGCAAACGTTTCATAGAACAACATCCCTCCGCTTCTTTTTggcaggcc            <  5:14851/138‑2 (MQ=255)                                                                                                                 cGCTGAGCCGAATAGACCTCTGCAGCCGCAGTGTTTCTGTCCTGCGTCACCCAGGTAATCAAAACGCCCATCCCCACACGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca            <  1:58078/138‑1 (MQ=255)                                                                                                                 cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca            <  1:89316/138‑1 (MQ=255)                                                                                                                 cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca            <  1:280277/138‑1 (MQ=255)                                                                                                                 cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca            <  7:48547/138‑1 (MQ=255)                                                                                                                 cGCTGAGCAGAATAGAACGCGGCAGCCGCAGTTTTTCTGTACGGCGTCACACAGTTCATCAAAAAGACCATCCACACCCGTAAATATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca            <  5:8099/138‑1 (MQ=255)                                                                                                                    tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca            <  8:273502/135‑1 (MQ=255)                                                                                                                    tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca            >  7:273502/1‑135 (MQ=255)                                                                                                                    tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCCGGCAGCCt        >  7:322853/1‑139 (MQ=255)                                                                                                                    tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCAGCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCt        >  4:408238/1‑139 (MQ=255)                                                                                                                     gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTGTTTTTGGCAGGCAGCCtt       >  7:29279/1‑139 (MQ=255)                                                                                                                     gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCtt       >  4:104020/1‑139 (MQ=255)                                                                                                                     gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCtt       >  5:130744/1‑139 (MQ=255)                                                                                                                           aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCtttttag  >  1:409288/1‑135 (MQ=255)                                                                                                                                  |                                                                                                                                   ATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTAGC  >  CP006881/3724953‑3725212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑