Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	331,203	T→G	10.0%	L143R (CTG→CGG)	U712_01580 →	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	331,203	0	T	G	10.0%	166.8 / 2.8	72	L143R (CTG→CGG)	U712_01580	L‑lactate permease
Reads supporting (aligned to +/- strand):  ref base T (32/32);  new base G (4/4);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTACCATTATTCGGTCCAGCATTGCCGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCAGATTGGTGATTTGTCTGCTCTTGAG  >  CP006881/331066‑331339                                                                                                                                          |                                                                                                                                         aTTTACCATTATTCGGTCCACCATTGCCGGCATTTCGCCTGACCAACGATTACAGATATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGACGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCgctg                                                                                                                                         <  1:144382/139‑1 (MQ=255)    tACCATTATTCGGTCCAGCATTGCCGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCtc                                                                                                                                      >  1:187939/1‑139 (MQ=255)           attCGGTCCAGCATTGCCGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAAc                                                                                                                               >  6:71647/1‑139 (MQ=255)            ttCGGTCCAGCATTGCCGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGACGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACt                                                                                                                              <  1:115977/139‑1 (MQ=255)            ttCGGTCCAGCATTGCCGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTGCGATTGGGGTTAGTGCGGCGCGGGTCGTCGGACt                                                                                                                              >  1:129004/1‑139 (MQ=255)            ttCGGTCCAGCATTGCCGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACt                                                                                                                              <  2:187939/139‑1 (MQ=255)             tCGGTCCTGCTTTGCTGGCATTTCGCCTGACCAACGATTACAGCTATTATTATTTGGTTTTTTTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACt                                                                                                                              <  2:125731/138‑1 (MQ=255)             tCGGTCCAGCATTGCCGGGATTTCGACTGACCAACGTTTACAGCTATTATTATTTGGTTTTTGTTTTAACGGGTTTTTACAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACt                                                                                                                              <  5:269694/138‑1 (MQ=255)                gTCCAGCATTGCCGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGGATGCGATTAGTGTTGCGCTGCTCGTCGAAATTGGt                                                                                                                          >  1:64079/1‑139 (MQ=255)                 tCCAGCATTGCCGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGGTCCGATTTCGATTAGTGCGGCGCTGCTGGTCGAACTTgggt                                                                                                                         >  1:288517/1‑137 (MQ=255)                 tCCAGCATTGCCGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGGTTTGGTGTTCCGCTTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGtt                                                                                                                         >  7:96379/1‑139 (MQ=255)                 tCCAGCATTGCCGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGGGTTGGTGGTCCGCTTGCGATTTGTGCGGCGCGGGTCGGCGAACTTGGtg                                                                                                                         >  4:63390/1‑138 (MQ=255)                    aGCATTGCCGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTTACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGGTCGTCGACCTTGGTTGTa                                                                                                                      >  6:101683/1‑139 (MQ=255)                     gCATTGCCGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTaa                                                                                                                     <  5:116239/139‑1 (MQ=255)                      cATTGCCGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCAGGTCGAACTTTGTTTTaaa                                                                                                                    >  7:142403/1‑139 (MQ=255)                          gCCGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCgctg                                                                                                                                         <  4:50124/114‑1 (MQ=255)                          gCCGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCgctg                                                                                                                                         >  3:50124/1‑114 (MQ=255)                           ccGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTGCGATTGCGATTAGTGCGGCGCTGCTCGTCGCACTTGGGTTTAAAccct                                                                                                                >  5:335440/1‑136 (MQ=255)                           ccGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGCTCTTGGTTTTAAACCGt                                                                                                                >  8:169801/1‑138 (MQ=255)                           ccGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGtt                                                                                                               >  5:117443/1‑139 (MQ=255)                           ccGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGt                                                                                                                >  2:49901/1‑138 (MQ=255)                           ccGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGt                                                                                                                >  7:333423/1‑138 (MQ=255)                           ccGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGt                                                                                                                >  4:151401/1‑138 (MQ=255)                           ccGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGGGTTGCGATTGCGATTTGTGCGGCGCGGCTCGTCGAACTTGGTTTTAAACCGtt                                                                                                               >  6:22830/1‑139 (MQ=255)                           ccGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGGGTTCCGATTGCGCTTAGTGCGGCGCTGCGCGTCGAACTTGCTGTTAAACCGt                                                                                                                >  3:103770/1‑138 (MQ=255)                           ccGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTATTTGTTTTAACGCGTTTTTAGAAGTCGCGGCCGGATTTTGGGTTACGATTTCGATAATTGCGGTTCTGCTCGTCGAAGATTGTTTTAAACCGt                                                                                                                >  4:9869/1‑138 (MQ=255)                           ccGGCATTTCGCCTGACCAACCATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTATTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGACCTTGGGTTTAAACAGt                                                                                                                >  7:214915/1‑138 (MQ=255)                                aTTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAg                                                                                                          >  5:167980/1‑139 (MQ=255)                                aTTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAg                                                                                                          <  8:333423/139‑1 (MQ=255)                                      ccTTACAAACGATTACAGGTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAATAgcggcgg                                                                                                    <  8:214915/139‑1 (MQ=255)                                      ccTGACCCACGATTACAGCTCTTATTAATGGGTTTTTTTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCTAACTTGGTTTTAAACCGTTAAAAgcggcgg                                                                                                    <  5:153473/139‑1 (MQ=255)                                         gACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTTGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAgcggcggcg                                                                                                  >  6:326156/1‑138 (MQ=255)                                          aCCAACTATTACAGCTATTATTAATTGTTTTTTTTTTTAACGCTTTTTTAGACGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAgcggcggcg                                                                                                  <  6:117443/137‑1 (MQ=255)                                          aCCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAgcggcggcg                                                                                                  <  3:174805/137‑1 (MQ=255)                                          aCCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGct                                                                                                <  1:4690/139‑1 (MQ=255)                                          aCCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGct                                                                                                >  1:339974/1‑139 (MQ=255)                                           ccAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAAGGCGTTTTACGACGGCGCGGCCGGTTTTTGTGTTCAGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGct                                                                                                <  2:7313/138‑1 (MQ=255)                                               cGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGTTTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCt                                                                                           <  6:192442/139‑1 (MQ=255)                                               cGATTAAAGCTATTATTAATTGGTTTTTGTTTTAAGGCGTTTTAAGACGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCt                                                                                           <  3:190516/139‑1 (MQ=255)                                                     catctcttattaCTGGGTTTTTTTTTTAACGGGTGTTTCTAAGGCGCGGCCGGGTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTg                                                                                     <  4:103770/133‑1 (MQ=255)                                                          attattAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACt                                                                                                                              <  3:60546/93‑1 (MQ=255)                                                          attattAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACt                                                                                                                              >  4:60546/1‑93 (MQ=255)                                                           ttattaATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACg                                                                               >  6:132753/1‑139 (MQ=255)                                                             attaATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCt                                                                             >  8:300307/1‑139 (MQ=255)                                                              ttaTTTGTTTTTTTTTTTAACGCGTTTTTACCCGGCGCGGCCGGTTTTGGTGTTCCGTTTGCGTTTAGTGGGGCGGTGGTCGTCGAAGTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCt                                                                             <  1:238306/138‑1 (MQ=255)                                                              ttaATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCt                                                                             <  6:158542/138‑1 (MQ=255)                                                              ttaATTGGTTTTTGTTTTAACGCGGTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCt                                                                             <  7:300307/138‑1 (MQ=255)                                                                aaTTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCGGGTCGCACTTGGTTTTTTACCGTTTAAAGCGGCGGCGCTCTGGTGGTTTGCCACCGCGGcc                                                                          >  4:203731/1‑139 (MQ=255)                                                                  ttGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTc                                                                        >  7:161698/1‑139 (MQ=255)                                                                       ttttGTTTTACGGCGTTTTATGAGGGCGCGGCGGGTTTTGGTGTGCGTTTTGCGATTAGGGCGGCGCTGCTCGTCTAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGa                                                                    <  2:339974/138‑1 (MQ=255)                                                                        tttGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGc                                                                  >  1:8931/1‑139 (MQ=255)                                                                        tttGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGGTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCGCCGGAGc                                                                  >  5:213827/1‑139 (MQ=255)                                                                                      tttttAGAAGGCGCGGCCGGGTTTGGTGTTCCGATTGCGAATAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTg                                                    >  7:20465/1‑139 (MQ=255)                                                                                            gAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGAtt                                               <  6:167980/138‑1 (MQ=255)                                                                                            gAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGAtt                                               <  1:49901/138‑1 (MQ=255)                                                                                              aGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCt                                            <  5:71647/139‑1 (MQ=255)                                                                                              aGGCGCGGCCGGTTTTGGGGTGCCGATGGCGATTAGTGCGGCGCGGCTCGTCGAACTTGGTTTTAAACCGTTAAACGCGGCGGCGCGCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCt                                            <  3:94620/139‑1 (MQ=255)                                                                                               ggCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTg                                           >  7:41900/1‑139 (MQ=255)                                                                                               ggCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAAACGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTg                                           >  3:46238/1‑139 (MQ=255)                                                                                                gcgcGGCCGGTTTTGGTGTTCCGATTGCGATTTGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGt                                          <  4:74981/139‑1 (MQ=255)                                                                                                       cGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATcaca                                   <  2:129004/139‑1 (MQ=255)                                                                                                         ggtttGGGGTTGCGATTGCGATTAGTGCGGCGCGGCTCGTGGAACTTGGTTTTAAACCGTTTAAAGCGGCGGCGCTCGGCTTGATTGCAAACGCTGCCGCCGGGGCCTTTGGGGGGGTTGGGTTTCCTGTCATCACAgg                                 >  4:238586/3‑139 (MQ=255)                                                                                                              ggTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGgcg                             <  8:161698/138‑1 (MQ=255)                                                                                                              ggTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGgcg                             <  6:335440/138‑1 (MQ=255)                                                                                                               gtgtTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCa                           <  6:213827/139‑1 (MQ=255)                                                                                                                 gtTCCGATTGCGATTAGTGCGGCGCGGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCAGa                         <  1:151860/139‑1 (MQ=255)                                                                                                                  ttCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCAGa                         <  3:151401/138‑1 (MQ=255)                                                                                                                    ccGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCAGATTg                      >  1:343471/1‑139 (MQ=255)                                                                                                                    ccGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCAGATTg                      >  3:365072/1‑139 (MQ=255)                                                                                                                    ccGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCAGATTg                      >  3:274497/1‑139 (MQ=255)                                                                                                                         tGCGAGTAGGGCGGCGCGGCTCGGCGAACTTGGTTTTAAACCGTAAACAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCAGATTGGGGAt                 <  2:128523/139‑1 (MQ=255)                                                                                                                             aTTAGTGCGGCGCTGCTGGGCGAACTTGGTTTTAAACCGTTAAACGCGGCGGCGCTCTGCTTGATTGCAATCGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCAGATTGGTGAttttt             <  6:154502/139‑3 (MQ=255)                                                                                                                              ttAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAACGCGGCGGCGCTCTGCTTGATTGCAATCGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCAGATTGGTGATTTGTc            <  2:288517/139‑1 (MQ=255)                                                                                                                                 gTGCGGCGCGGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCAGATTGGTGATTTGTCTg          <  5:22830/138‑1 (MQ=255)                                                                                                                                   gcggcgCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCAGATTGGTGATTTGTCTGctc       <  2:8931/139‑1 (MQ=255)                                                                                                                                        gctgctCGTCGAACTTGGTTTTACACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCAGATTGGTGATTTGTCTGCTCTTGAg  <  2:64079/139‑1 (MQ=255)                                                                                                                                        gctgctCGTCGAACTTGGTTTTAAACCGTTAAAAGGGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCAGATTGGTGATTTGTCTGCTCTTGAg  <  2:247354/139‑1 (MQ=255)                                                                                                                                        gctgctCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCAGATTGGTGATTTGTCTGCTCTTGAg  <  7:169801/139‑1 (MQ=255)                                                                                                                                        gctgctCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCAGATTGGTGATTTGTCTGCTCTTGAg  <  3:238586/139‑1 (MQ=255)                                                                                                                                        gctgctCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTGTGGGGCTATTGGGATTCCTGTCATCACAGGGGCGCAGATTGGTCATTTGTCTGCTCATGAg  <  5:326156/139‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         ATTTACCATTATTCGGTCCAGCATTGCCGGCATTTCGCCTGACCAACGATTACAGCTATTATTAATTGGTTTTTGTTTTAACGCGTTTTTAGAAGGCGCGGCCGGTTTTGGTGTTCCGATTGCGATTAGTGCGGCGCTGCTCGTCGAACTTGGTTTTAAACCGTTAAAAGCGGCGGCGCTCTGCTTGATTGCAAACGCTGCCTCCGGAGCCTTTGGGGCGATTGGGATTCCTGTCATCACAGGGGCGCAGATTGGTGATTTGTCTGCTCTTGAG  >  CP006881/331066‑331339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑