Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 485,016 A→T 16.6% intergenic (+42/‑106) U712_02265 → / → U712_02270 C4‑dicarboxylate transport protein/UPF0118 membrane protein ydbI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881485,0160AT16.6% 141.6 / 4.6 56intergenic (+42/‑106)U712_02265/U712_02270C4‑dicarboxylate transport protein/UPF0118 membrane protein ydbI
Reads supporting (aligned to +/- strand):  ref base A (4/42);  new base T (8/2);  total (12/44)
Fisher's exact test for biased strand distribution p-value = 1.34e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACGATCATTGTTGCCAAAAGCGAAAATGAATTTGATGAAGCAAAAAGCATAGAGGCTGTTGAAGGCATGAAAAAAATGAAAACAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAGGAGTGTATAAATGGCCGCTTTAGTTAAATTTTTTCAGCACCC  >  CP006881/484880‑485153
                                                                                                                                        |                                                                                                                                         
ccaccaTCATTTTTGCCAAAAGCTAAAATTAATTTGATCAACCAAAAAGCATAGAGGCTTTTGAAGGAATCAAAAAAATGAAAACACCAGTCTCAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAg                                                                                                                                         <  3:63573/139‑1 (MQ=255)
ccACGATCATTGTTGCCAAAAGCGAAAATGAATTTGATGAAGCAAAAAGCATAGAGGCTGTTGAAGGCATGAAAAAAATGAAAACAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAg                                                                                                                                         <  7:186498/139‑1 (MQ=255)
       cATTTTTGCCAAAAGCTAAAATGAATTTTATCAACAAAAAAGAAATGCGGCTGTTGAAGCCATAAAAAAAATGAAAACAGCAGTCTCAAAAAAGCACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAattatt                                                                                                                                  <  8:320087/139‑1 (MQ=255)
       cATTGTTGCAAAAAGCGAAAAATAATTAGATGAAGCAACAAGCAACGAGCCTGTTGACGCCATCAAAAAAATGAAAACAGCAGTCTGAAAAAAGACCGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAattatt                                                                                                                                  <  7:361138/139‑1 (MQ=255)
        aTTGTGTCCAAAAGCTAAAATTAATTTTTTGAAGAAAAATGCTTAGGGGCGGTGGAAGGCATTAAAAAAATGAAACCAGCAGTCTGAAAAAACAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAattatt                                                                                                                                  <  6:80020/138‑1 (MQ=255)
          tgttgCCAAAAGCGAAAATGAATTTGATGAAGCAAAAAGCATAGAGGCTGTTGAAGGCATGAAAAAAATGAAAACAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTTATTGTTTTAGAATAATtaag                                                                                                                               >  1:26946/1‑139 (MQ=255)
            ttgCCAAAAGCTAAATTGAATTTGATGAAGCAAAAAGCATAGCGGCTGTTGAAGGAATTAAAAAAATGAAAACAGCAGTCTTAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATtaaggt                                                                                                                             <  7:56243/139‑1 (MQ=255)
             tgCCAAAAGCTAAAATTATTTTGATCAAGAAAAAAGCATAGAGGCTGTTGAAGGCATTAAAAAAATGAAAACAGCAGTCTTAAAAAACAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATtaaggt                                                                                                                             <  6:248622/138‑1 (MQ=255)
             tgCCAAAAGCGAAAATGAATTTGAAGAAGCAAAAAGCATAGAGGCTGTTGAAGGAATGAAAAAAATGAAAACAGCAGTCTGAAAAAAGAACCGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATtaaggt                                                                                                                             <  5:88422/138‑1 (MQ=255)
              tcaaaaaGAGAAAATGAATTTGATGAAGAAAAAACCATAGAGGCTTTTGAAGGCATTAAAAAAATGAAAACAGCAGTCTTAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATtaaggta                                                                                                                            <  5:98808/135‑1 (MQ=255)
              gCCAAAAGCGAAAATGAATTTGATGAAGCAAAAAGCATAGAGGCTGTTGAAGGCATGAAAAAAATGAAAACAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTTATTTTTTAAGAATTATtaatgtaa                                                                                                                           >  6:330930/1‑139 (MQ=255)
              gCCAAAAGCGAAAATGAATTTGATGAAGCAAAAAGCATAGAGGCTGTTGAAGGCATGAAAAAAATGAAAACAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTGTTTTTTTTTTTTTTATAATTTTtaaatgaa                                                                                                                           >  7:355026/1‑134 (MQ=255)
              gCCAAAAGCGAAAATGAATTTGATGAAGCAAAAAGCATAGAGGCTGTTGAAGGCATGAAAAAAATGAAAACAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTGTTTTTTATTTTTTTATTTTTTTtaaggtta                                                                                                                           >  3:186273/1‑137 (MQ=255)
                   aaGCGAAAATGAATTTGATGAAGCAAAAAGCATAGAGGCTGTTGAAGGCATGAAAAAAATGAAAACAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACa                                                                                                                      <  5:145462/139‑1 (MQ=255)
                     gagAAACTTAATTTGATTAACAAAAAAGCATATAGCCTGTTGAAGGCATTAAAAAAATTAAAAAAGCAGGATGAAAAAACAACGGCACATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACaga                                                                                                                    <  5:170647/137‑1 (MQ=255)
                          aattaattTCACGAACCAAAAACCATAGAGGCTGTTGAAGGCATCAAAAAAATCAAAACACCAGCCTGAAAAACGCACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCt                                                                                                               <  1:300326/139‑1 (MQ=255)
                                            aaaaGCATAGAGGCTGTTGAAGGCATGAAAAAAATGAAAACAGCAGTCTGAAAAAAGCACGGCTCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTtata                                                                                             <  3:271468/139‑1 (MQ=255)
                                            aaaaGCACTTAGGATGTTTAAGGCTTTAAAAAAATGAAAACACAATTCTGAAAAACGAACGGCCCATCCCTGGGCCGTTTTTTTAATTGTTTTAGAATTATTAAGTTCAGGACAGAGACCTCAGCATTTTTTTGTtata                                                                                             <  8:19969/139‑1 (MQ=255)
                                            aaaaGAATAGAGGCTGTTGAAGGCATGAAAAAAATGAAAACAGCAGTCTGAAAAAACAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTtata                                                                                             <  7:362070/139‑1 (MQ=255)
                                                   tAGAGGCTGTTGAAGGCATGAAAAAAATGAAAACAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTa                                                                                      <  4:144537/139‑1 (MQ=255)
                                                   tAGAGGCTGTTGAAGGCATGAAAAAAATGAAAACAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTGGTTTTAATTGTTTTAGAATTTTTAAGGTAAGGCCAGAGACCTGAGCATTTTTTTTTTATTTAAttt                                                                                       >  8:8648/1‑138 (MQ=255)
                                                   tAGAGGCTGTTGAAGGCATCAAAAAAATCAAAACAGCAGTCTGAAAAAACAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTa                                                                                      <  5:330930/139‑1 (MQ=255)
                                                      agGCTGTTGAAGGCATGAAAAAAATGAAAACAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTTATTTTTTAAGTATTTTTTATGTAATGACAGAGACCTCAGCATTTTTTTTTTTTTTAAttttaa                                                                                    >  4:341842/1‑135 (MQ=255)
                                                      agGCTGTTGAAGGCATGAAAAAAATGAAAACAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTAATTTTTTTAGAATTATTTATGTAAGGACACAGACCTCAGCATTTTTTTTTTatttaatttaaa                                                                                    >  4:347272/1‑136 (MQ=255)
                                                           gTTGAAGGCATGAAAAAAATGAAAACAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTTAATGTTTTATAAATATATATGTAAAGACAAAGCACAAATCAAATTTTTTTTTTATAAATTAaaaaattg                                                                              >  7:242341/1‑135 (MQ=255)
                                                           gTTGAAGGCATGAAAAAAATGAAAACAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTGTTTTTTATTGTTTTATAATTTTTTATGTAATGACAGAGACCTCAGCAGTTTTTTTGTATTTTATTTTAAAAtata                                                                              >  4:92822/1‑136 (MQ=255)
                                                            ttGAAGGCATTAAAAAAATGAAAACAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGAt                                                                             <  3:72483/139‑1 (MQ=255)
                                                            ttGAAGGCATGAAAAAACTCAAAACAGCATTCTTAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGAt                                                                             <  4:312224/139‑1 (MQ=255)
                                                              aaccccATAAAAAAATCCAAAACTCCTGTCTCAAAAAACAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATa                                                                            <  1:152374/133‑1 (MQ=255)
                                                                  ccATCAAAAAAATCAAAACAGCAGTCTCAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACtata                                                                       <  1:60542/138‑1 (MQ=255)
                                                                    ataaaaaaaaTAAAAACCGCAGTCTTAAAAAACAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACtata                                                                       <  4:186273/134‑1 (MQ=255)
                                                                            aaTGAAAACAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTGTTTTTAATTTTTTTAGAATTTTTATGGTAAGGACCTGGACGAGCGCATTTTTTTGTTTTATAATTTTAAAATGGTTACTATTTTGAATGAAg                                                             >  1:201088/1‑139 (MQ=255)
                                                                            aaTCAAAACACCAGTATGAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAg                                                             <  3:269579/139‑1 (MQ=255)
                                                                                 aaaCAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGt                                                        <  3:361358/139‑1 (MQ=255)
                                                                                 aaaCACCAGGCTGAAAAACGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGt                                                        <  6:45871/139‑1 (MQ=255)
                                                                                     cgcagCCTCAAAAAACCACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGt                                                    <  3:220910/138‑1 (MQ=255)
                                                                                     agcagTCTGAAAAAACCACCGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGt                                                    <  2:201088/139‑1 (MQ=255)
                                                                                     accagTATTAAAAACTAACGGCCCATCCATGGGCCTTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGt                                                    <  4:231665/137‑1 (MQ=255)
                                                                                       aagTCTGAAAAAAGCACGGCCCATCCATGGGGCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTcc                                                  <  3:255032/138‑1 (MQ=255)
                                                                                             caaaaaaCAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAg                                            <  5:213965/138‑1 (MQ=255)
                                                                                              aaaaaaGCACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAg                                            <  3:341842/138‑1 (MQ=255)
                                                                                              aaaaaaGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAg                                            <  3:347272/138‑1 (MQ=255)
                                                                                              aaaaaaGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAg                                            <  7:102069/138‑1 (MQ=255)
                                                                                              aaaaaaGAACGGCACCTCCATGGGCCGTTTTTTTAATTGGTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAg                                            <  4:238112/138‑1 (MQ=255)
                                                                                               aaaccgAACGGCCCATCCATGGGCCGTTTTTTTTATTGTTTTAGAATTATTACGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAGGa                                          <  1:317921/134‑1 (MQ=255)
                                                                                               aaaaaGCACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAGGa                                          <  2:140822/139‑1 (MQ=255)
                                                                                               aaaaaGAACTGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACTAATTGATACTATATTGAATGAAGGACGTTTGTCCTAACAGGa                                          <  5:186664/139‑1 (MQ=255)
                                                                                               aaaaaGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAGGa                                          <  4:229032/139‑1 (MQ=255)
                                                                                                         gCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAGGAGTGTATAAAt                                <  7:43073/139‑1 (MQ=255)
                                                                                                         gCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAGGAGTGTATAAAt                                <  7:48433/139‑1 (MQ=255)
                                                                                                                                 aTTTTTTAAGAATTATTAACGTAAGGACAGAGAACTCAGCATTTTTTTGTTATATAATTTACAATTGGATACTATATTGAATGAAGGACGTTTGTCCTAACAGGAGTGTATAAATGGCCGCCGAAGTTAAATTTTTTCa        <  1:265009/139‑1 (MQ=255)
                                                                                                                                  ttGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAGGAGTGTATAAATGGCCGCTTTAGTTAAATTTTTTCAg       <  2:266641/139‑1 (MQ=255)
                                                                                                                                     tttAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAGTTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAGGAGTGTATAAATGGCCGCTTTAGTTAAATTTTTTCAGCAc    >  1:326352/1‑139 (MQ=255)
                                                                                                                                       tAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAGGAGTGTATAAATGGCCGCTTTAGTTAAATTTTTTCAGCAccc  >  8:346966/1‑139 (MQ=255)
                                                                                                                                       tAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAGGAGTGTATAAATGGCCGCTTTAGTTAAATTTTTTCAGCAcc   <  1:108598/138‑1 (MQ=255)
                                                                                                                                       tAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAGGAGTGTATAAATGGCCGCTTTAGTTAAATTTTTTCAGCAcc   <  7:8648/138‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                         
CCACGATCATTGTTGCCAAAAGCGAAAATGAATTTGATGAAGCAAAAAGCATAGAGGCTGTTGAAGGCATGAAAAAAATGAAAACAGCAGTCTGAAAAAAGAACGGCCCATCCATGGGCCGTTTTTTTAATTGTTTAAGAATTATTAAGGTAAGGACAGAGACCTCAGCATTTTTTTGTTATATAATTTACAAATGGATACTATATTGAATGAAGGACGTTTGTCCTAACAGGAGTGTATAAATGGCCGCTTTAGTTAAATTTTTTCAGCACCC  >  CP006881/484880‑485153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑