Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 571,197 C→A 23.1% intergenic (‑89/+83) U712_02810 ← / ← U712_02815 putative transporter YdgF/Protein dinB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881571,1970CA23.1% 90.8 / 8.2 48intergenic (‑89/+83)U712_02810/U712_02815putative transporter YdgF/Protein dinB
Reads supporting (aligned to +/- strand):  ref base C (29/7);  new base A (2/9);  total (31/17)
Fisher's exact test for biased strand distribution p-value = 3.20e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACGTTTGCTGATTTATATTGTCTTTCGTCATGTCGTCTGTCACTTTATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGC‑TTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGT  >  CP006881/571064‑571327
                                                                                                                                     |                                                                                                                                    
cAACGTTTGCTGATTTATATTGTCTTTCGTCATGTCGTCTGTCACTTTATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTaaaa                                                                                                                                 <  7:165571/139‑1 (MQ=255)
      ttGCTGATTTATATTGTCTTTCGTCATGTCGTCTGTCACTTTATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGAt                                                                                                                          >  2:55722/1‑139 (MQ=255)
      ttGCTGATTTATATTGTCTTTCGTCATGTCGTCTGTCACTTTATGTGTTCATCCATTTAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATTATTATTATAACACTAAAATTTCTATATTTTTTAATAAAAAA‑GGAt                                                                                                                          >  7:283364/1‑139 (MQ=255)
        gCTGATTTATATTGTCTTTCGTCATGTCGTCTGTCACTTTATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GTATtg                                                                                                                        >  2:325091/1‑139 (MQ=255)
        gCTGATTTATATTGTCTTTCGTCATGTCGTCTGTAACTTTATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTTATGATTATTATAAAACTAAAATTTATATCTTTTTTACTAAAAAA‑GCAtt                                                                                                                         >  5:194941/1‑138 (MQ=255)
                      tCTTTCGTCATGTCGTCTGTCACTTTATGTGTTCCTCCATTGAAAATTGTTTATTTAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTATATCTTTTTTACTAAAAAA‑GGATTGATGAAAATAAttt                                                                                                           >  7:254796/1‑138 (MQ=255)
                      tCTTTCGTCATGTCGTCTGTCACTTTATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAAttt                                                                                                           >  5:114750/1‑138 (MQ=255)
                           cGTCATGTCGTCTGTCACTTTATGTGTTACTCCATTGAAAATTGTTTATTGAGTCAGAAATAAATTTTTAATTATTATTATAACACTAAAATTTCTATAATTTTTAATAAAAAA‑TGATTGATGAAAATAATTTTAAttt                                                                                                     >  2:33708/1‑137 (MQ=255)
                                tGTCGTCTGTCACTTTTTGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTTTTTTAACACTAAAATTTATTTATTTTTTTCTAAAAAA‑GGATTTATTAAAATAATTTTAATTTGAttt                                                                                                >  2:176449/1‑139 (MQ=255)
                                tGTCGTCTGTCACTTTATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTTAttt                                                                                                >  5:316325/1‑139 (MQ=255)
                                tGTCGTCTGTCACTTTATGTGTTACTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATTTGAttt                                                                                                >  4:144903/1‑139 (MQ=255)
                                         tCACTTTATGAGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTAt                                                                                         <  4:65710/137‑1 (MQ=255)
                                              ttATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCg                                                                                   >  8:215978/1‑138 (MQ=255)
                                               tATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGa                                                                                  >  5:47849/1‑138 (MQ=255)
                                                       cctccATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GTATTGATTAAAAGAATTTTAATGTTATTTGGACTATTTACCTAAAAAAGCgg                                                                         >  3:223420/1‑139 (MQ=255)
                                                       cctccATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAATCgg                                                                         >  8:92495/1‑139 (MQ=255)
                                                       cctccATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCgg                                                                         >  4:116269/1‑139 (MQ=255)
                                                       cctccATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCgg                                                                         >  1:335450/1‑139 (MQ=255)
                                                       cctccATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCgg                                                                         >  8:310597/1‑139 (MQ=255)
                                                       cctccATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCgg                                                                         >  5:195104/1‑139 (MQ=255)
                                                       cctccATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCgg                                                                         >  7:100479/1‑139 (MQ=255)
                                                        ctccATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTAACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGGAAAAAGCGGc                                                                        <  8:225978/139‑1 (MQ=255)
                                                             ttGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCAt                                                                   >  5:103948/1‑139 (MQ=255)
                                                                  aaTTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTATTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTatg                                                              >  8:202707/1‑137 (MQ=255)
                                                                        ttATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCg                                                        >  2:36719/1‑139 (MQ=255)
                                                                         tATTGAGTAACCAATCTATTTTTAATGATTTTTAAAACAATAAAATTTATATATTTTTAACTAAAAAC‑GGATTCATTAAAATATTTTTATTGTTATTTGGACTATTCCCCAAAAAAAGCGGCTCCATTTAGGGAGCCg                                                        <  2:59967/138‑1 (MQ=255)
                                                                                tCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTACATTTAGGGAGCCGC‑gtttttt                                               >  7:216250/1‑138 (MQ=255)
                                                                                         tATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGCGGTTTTTTTGtttttt                                       >  4:204801/1‑139 (MQ=255)
                                                                                         tATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATATCCCGAAAAAAGCGGCACCATTTAGGGAGCCGCGTTTTTTATgtgttt                                        >  2:191959/1‑133 (MQ=255)
                                                                                              ttAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGC‑TTTTTTTTTGTTTTTAAtttt                                 >  5:280781/1‑139 (MQ=255)
                                                                                               ttattattattATAACAATAAATTTTATATCTTTTTTACTAAAAAA‑GGATTGATTAAAAGAATTTTATTGTTTTTTGGACTTTTACCCGAAAAAAGCGGCTCCATTTAGGGAGCCGC‑TTTTTTATGCTTTTTCAGttt                                 <  7:202707/133‑1 (MQ=255)
                                                                                               tAATGATTATTATAAAACTAAAATTTCTATCTTTTTTAAAAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGAATATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGC‑TTTTTTATGCTTTTTCAGttt                                 <  3:204801/138‑1 (MQ=255)
                                                                                                     ttattaTACAACTAAAATTTATATATTTTTTAATAAAAAT‑GTATTTATGAAAATATTTTTATTGTGATTTGGAATATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGC‑TTTTTTATGCTTTTTCAGTTTTTGTCa                           <  8:100479/138‑1 (MQ=255)
                                                                                                     ttattaTAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGCGGTTTTTTTGTTTTTAAAGttttttaa                            >  4:114838/1‑135 (MQ=255)
                                                                                                     ttattaTAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGC‑GTTTTTTTGTGTTTTTAtttttttttat                          >  4:115047/1‑134 (MQ=255)
                                                                                                     ttattaTAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGC‑GTTTTTATGGTTTTTCAGTTTTTTTCa                           >  8:306723/1‑138 (MQ=255)
                                                                                                     ttattaTAACAAAAATATTTATTTTTTTTTTAATAAAAAT‑GGTTTTATTAAAATAATTTTATTGTGTTTTGTAATATTTCCCTAAAAAAGCGGCTCCATTTAGGGAGCCGC‑TTTTTTATGCTTTTTCAGTTTTTGTCa                           <  6:103948/138‑1 (MQ=255)
                                                                                                               ataaaaaTTTCTATTTTTTTTAAAAAAAAT‑GTATTGATTAAAATAATTTTAATGTTATTTGTACAATTACCCGAAAAAAGCGGCTCCATTTAGGGAGCCGC‑TTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTag                <  6:280781/136‑1 (MQ=255)
                                                                                                                cTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGC‑GTTTTTTTTCTTTTTGAGTTTTTTTCATTAAAAGGTact               >  6:257288/1‑137 (MQ=255)
                                                                                                                  aaaaTTTATCTCTTATTAAAAAAAAAA‑TTATTTATTAAAATAATTTAAATGTGATTTTCAAAATTTCCCTAAAAACGCGGCTCCATTTAGGGAGCCGC‑TTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGc             <  8:164081/139‑1 (MQ=255)
                                                                                                                   aattttATATTTTTTTAAATAAAAAC‑TTTTTTATAAAAATATTTTTATTGTTTTTTGAAATATTACCCAAAAAACGCGGCTCCATTTAGGGAGCCGC‑TTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGcc            <  3:114838/136‑1 (MQ=255)
                                                                                                                   aaaTTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAACGCGGCTCCCTTTAGGGAGCCGC‑GGTTGTTTTGGTTTTGGGTTTTTGTCGTAAACACGTGGAGcc            >  8:169700/1‑139 (MQ=255)
                                                                                                                   aaaTTTATTTCTTTTTAAATAAAAAT‑GTTTTTATAAAAATAATTTTAATTTTATTTGAAATATTACCATAAAAACGCGGCTCCATTTAGGGAGCCGC‑TTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGcc            <  1:33708/139‑1 (MQ=255)
                                                                                                                   aaaTTTATATCTTTTTAAATAAAAAA‑GGATTCATCAAAATAATTTTAATGTGATTTGGACAATTGCCCTAAAAACGCGGCTCCATTTAGGGAGCCGC‑TTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGcc            <  7:306723/139‑1 (MQ=255)
                                                                                                                           ttattttttATAAAAAAA‑GTATTTATGAAAATAATTTTATTGTTTTTTGGAATTTTACCATAAAAAAGCGGCTCAATTTAGGGAGCCGC‑TTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATc     <  6:316325/135‑1 (MQ=255)
                                                                                                                             tttttttAAAAAAAAATTGATTGAACAAAATATTTTTATTGTTATTTGCAATATTCCCCCAAAAACGCGGCTCCATTTAGGGAGCCGC‑TTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAAt      <  3:49473/135‑1 (MQ=255)
                                                                                                                                ttttACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTACCCGAAAAAAGCGGCTCCATTTAGGGAGCCGC‑TTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGt  <  8:303132/136‑1 (MQ=255)
                                                                                                                                tttaACTAAAAAT‑GTATTCATCAAAATAATTTAAATGTGATTTGGCCTATTACCCCAAAAACGCGGCTCCATTTAGGGAGCCGC‑TTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGt  <  8:77128/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
CAACGTTTGCTGATTTATATTGTCTTTCGTCATGTCGTCTGTCACTTTATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAA‑GGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGC‑TTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGT  >  CP006881/571064‑571327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑