Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,725,049 T→C 100% K40K (AAA→AAG)  U712_19205 ← Uncharacterized protein ywcI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,725,0490TC100.0% 127.2 / ‑6.6 40K40K (AAA→AAG) U712_19205Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (10/29);  minor base A (0/1);  total (10/30)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTC  >  CP006881/3724916‑3725183
                                                                                                                                     |                                                                                                                                      
ttGGCGATTCCTTGAAGGCTTGCAAACATCTGCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCCCCATCCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt                                                                                                                                   <  4:108913/139‑1 (MQ=255)
  ggCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                                 <  8:263802/139‑1 (MQ=255)
  ggCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                                 <  3:214229/139‑1 (MQ=255)
   gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                                 <  8:98911/138‑1 (MQ=255)
   gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                                 <  6:301406/138‑1 (MQ=255)
             gAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGACACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg                                                                                                                       <  8:91692/138‑1 (MQ=255)
              agggCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                                                     <  7:213152/137‑1 (MQ=255)
                ggCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGa                                                                                                                   <  3:190765/139‑1 (MQ=255)
                 gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                                  <  1:319108/139‑1 (MQ=255)
                 gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                                  <  8:181386/139‑1 (MQ=255)
                 gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                                  <  2:129576/139‑1 (MQ=255)
                 gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                                  <  4:180928/139‑1 (MQ=255)
                 gCTTGCAAAAATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                                  <  6:90332/139‑1 (MQ=255)
                      cAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGaa                                                                                                             <  5:84371/139‑1 (MQ=255)
                                cttTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACCGCCGCCGTTGTTTTGTATTTTAGCAGAGCCGAATAGACCGccg                                                                                                   >  5:173606/1‑137 (MQ=255)
                                     ataAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTgg                                                                                                                               <  4:345736/106‑1 (MQ=255)
                                     ataAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTgg                                                                                                                               >  3:345736/1‑106 (MQ=255)
                                     ataAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATACACCGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                                               <  2:125917/138‑1 (MQ=255)
                                      taAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                                             <  3:190632/139‑1 (MQ=255)
                                      taAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                                             <  7:218522/139‑1 (MQ=255)
                                      aaaaGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCAACCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                                             <  1:138551/138‑1 (MQ=255)
                                              ggcggcTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGttttt                                                                                     <  8:247543/139‑1 (MQ=255)
                                                   cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                                <  5:175822/139‑1 (MQ=255)
                                                             ctgcttttCTTCCTCATTCACCGCCAGCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCTCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCAc                                                                       <  8:304392/134‑1 (MQ=255)
                                                                          tCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTGTTTGGTATTTACGCGGAGCCGCATAGACCGCGGCAGCCGCCGGTTTTTGGTCCGGCGTCACCCAGGTGATCaaa                                                          >  3:62099/1‑138 (MQ=255)
                                                                                   gCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATcc                                                <  3:17932/139‑1 (MQ=255)
                                                                                    ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                               <  1:121353/139‑1 (MQ=255)
                                                                                    ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                               <  5:283664/139‑1 (MQ=255)
                                                                                    ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGGCACCCAGTTCATCAAAAAGACCATCca                                               <  7:28091/139‑1 (MQ=255)
                                                                                      aTCCGCCATTCATAACACGAATCGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGGTTTTCTGTCCGGCGGCACCCAGTTCATCAAAAAGACCATCcaca                                             <  8:351555/139‑1 (MQ=255)
                                                                                      aTCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCcaca                                             <  4:224426/139‑1 (MQ=255)
                                                                                              ttCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGGTCATCACAAAGGCCATCCACACCCgga                                       >  1:231886/2‑135 (MQ=255)
                                                                                              ttCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTCTTTGGTATGTAAGCGGCGCCGAATAGACCGCGGCCGCCCCAGTTTTTCTGTCCGGCGTCCCCCACTTCATCAAAAAGACCCACCACACCCCTaa                                      >  5:132908/2‑138 (MQ=255)
                                                                                                  tAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATAAAAAATACCATCCACACCCGTAAAGATa                                 <  3:174108/139‑1 (MQ=255)
                                                                                                  tAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGATGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATa                                 <  4:126397/139‑1 (MQ=255)
                                                                                                  tAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCATTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATa                                 <  7:162821/139‑1 (MQ=255)
                                                                                                       cGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGc                            <  6:173606/139‑1 (MQ=255)
                                                                                                          aaCGAGTCCTGCGGCCTGCACAGCCGCCGTTCTTTGGTATGTACCCTGAGCCGAATAGACACCGGCAGCCCCAGTTTTTATGTCAGTCGTCCCCCAGGTAATCAAAAATACCAACCACACCCGTAAAGATACAAGCaaa                         >  7:300107/1‑139 (MQ=255)
                                                                                                                         cTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaaaaa          <  3:299626/139‑1 (MQ=255)
                                                                                                                         cTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaaaaa          <  6:137721/139‑1 (MQ=255)
                                                                                                                          tGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGCATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGGAAAGATAAAAGCCAACCTTTGATTGaaaaa          >  4:66078/1‑138 (MQ=255)
                                                                                                                                 ccgcCTTTGTTTGGTATTTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTGCGGCGTCACCCAGTTCATGAAAAAGACCCTCCACCCCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTc  >  2:108810/1‑139 (MQ=255)
                                                                                                                                 ccgcCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTGCGGCGTCACCCCGTTCATCAAAAAGCCCATCCACACCCGGAAAGATACAATCAAAAGTTTCATAGAAAACCATCCCTc  >  4:249724/1‑139 (MQ=255)
                                                                                                                                 ccgcCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTc  >  1:34390/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTC  >  CP006881/3724916‑3725183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑