| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,796,099 | T→G | 11.3% | K317N (AAA→AAC) | U712_19580 ← | Cytochrome d ubiquinol oxidase subunit 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,796,099 | 0 | T | G | 11.3% | 124.7 / 2.0 | 56 | K317N (AAA→AAC) | U712_19580 | Cytochrome d ubiquinol oxidase subunit 1 |
| Reads supporting (aligned to +/- strand): ref base T (18/30); new base G (5/2); total (24/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATTTGCTGTTTTCAAGCTTTTTACGGCGGTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGCCAAGTAGCTCAAGGCATAAGGAACTTTGATTTCATTTGAGCTTTTTTCATTTTTTGTATCGAT > CP006881/3795964‑3796236 | agacaagCTGTTTTCAAGCTTTTTACGGCGGTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCtttt < 1:27348/133‑1 (MQ=255) aTTTGCTGTTTTCAAGCTTTTTACGGCGGTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCATGACAACCCCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCgtttgc > 3:330510/1‑137 (MQ=255) aTTTGCTGTTTTCAAGCTTTTTACGGCGGTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCGTTTcc > 1:156647/1‑139 (MQ=255) tttGCTGTTTTCAAGCTTTTTACGGCGGTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCAGGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTGACTGGCGGGTAGTAGTCGCCtgttggg > 1:344352/1‑136 (MQ=255) ttCAAGCTTTTTACGGCGGTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCGCTGGCGGTATGTAGTCGCCGTTTCGGTGTAttttt > 2:319543/1‑139 (MQ=255) gCTTTTTACGGCGGTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTa < 5:87561/139‑1 (MQ=255) tttACGGCGGTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCATGACAACCCCTGCGCCTACCATGCTGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCGTTTCCGTATATTTTTTCGTACTc < 6:120414/138‑1 (MQ=255) tttACGGCGGTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACt < 1:109717/137‑1 (MQ=255) tttACGGCGGTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACt < 5:152235/137‑1 (MQ=255) tttACGGCGGTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTc < 8:270262/138‑1 (MQ=255) tttACGGCGGTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTc < 6:160105/138‑1 (MQ=255) tttACGGCGGTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTc < 2:156647/138‑1 (MQ=255) tttACGGCGGTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTc < 4:94573/138‑1 (MQ=255) gcggTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGa < 8:64860/139‑1 (MQ=255) cggTTTAACCATAGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGaa < 5:139623/139‑1 (MQ=255) cggTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGaa < 2:292067/139‑1 (MQ=255) ccAAAGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTc < 3:201812/139‑1 (MQ=255) cAAACGCCGCCTAACGCAGCAAGACTCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCGTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCa < 8:298009/139‑1 (MQ=255) aaaGGCCTCCTAACGCCGCAAGAATCCTGAGAACTCCTGCTCCTAGCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCAt < 5:242818/139‑1 (MQ=255) aGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCtt > 3:257216/1‑97 (MQ=255) aGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCtt < 4:257216/97‑1 (MQ=255) caAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCt > 1:49292/1‑139 (MQ=255) caAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCt > 8:161686/1‑139 (MQ=255) aTGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTACGTTTATTTTTTGGTACGCAGCCTGAAGGGTTTTGATCCCTTTGACACTGACGCGGAAtttgt > 2:316729/1‑137 (MQ=255) gACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGaa > 6:357336/1‑101 (MQ=255) gACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGaa < 5:357336/101‑1 (MQ=255) gACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACAGGCGGAATGTAGTCGCCATTTCCGGATATTTTTTCGTAATCAGCCATCAGGGGTGTAATCCCTTTGACACGTCCGATGAAATTCTg > 5:169415/1‑138 (MQ=255) acaacaCCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGAt < 7:67603/139‑1 (MQ=255) acaacaCCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGAt < 3:100121/139‑1 (MQ=255) acaacaCCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCGTTTACGGATATTTTTTAGTGCTAAGCCGTAAGGGTTTTCATCCCTTTTAAAAGTCCGCAGAATTTCTGa > 4:84917/1‑138 (MQ=255) caacaCCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGAt < 1:164034/138‑1 (MQ=255) cacCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGc > 5:138003/1‑139 (MQ=255) cacCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTACGTATATTTTTTAGTACTCAGCCTGAAGGGTTTTAATCCCTTTGACACTTGCGCTGAATTTCTGATAAGc > 8:167844/1‑139 (MQ=255) acCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGcc < 8:7009/139‑1 (MQ=255) ccTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGCCa < 6:138003/139‑1 (MQ=255) gCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAgg > 8:12370/1‑93 (MQ=255) gCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAgg < 7:12370/93‑1 (MQ=255) gCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCGTTTGCGTATATTTTTTGGGACTCAGCCTGGAGGGTTTTGAGACCTTTGACACTTCCGCTGAATTTGTGATAAGGCAAGt > 6:109778/1‑139 (MQ=255) gCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATCTAGTCGCCGTTTGCGTATATTTTTTAGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGCCAAGt > 7:186170/1‑139 (MQ=255) cTCCTACCCTGATGCGGAAGCGCCAGAATGTCGTTTTCACTGGCGGAATGTAGGCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGCCAAg < 4:245213/137‑1 (MQ=255) cTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGCCAAg < 3:84917/137‑1 (MQ=255) aCCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTc < 2:233906/93‑1 (MQ=255) aCCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTc > 1:233906/1‑93 (MQ=255) aCCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCGTTTACCCATCCGCGGAATTTCTGATAAGCCAAGTAGCTc > 1:97076/1‑138 (MQ=255) aCCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTAAGCCTGAAGGGGTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGCCAAGTAGCTc > 3:332542/1‑138 (MQ=255) aCCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTAGTAAACAACCTGAAGGGGTTTCATCCCTTTTACACATACGCTGAAATTGTGATAAGACAAATAGCTc > 2:680/1‑138 (MQ=255) aCCATGATGCGGAAGCTCCAGAATGTCGTGTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTTCTCAGCCTGAAGGGTTTTCATCCCTGTGACACTTCCGCTGCATTTCTGCTAAGCCAAGTAtctc > 1:96246/1‑138 (MQ=255) aCCATGATGAGGAAGCTCCAGAATGTCTTTTTCAGTGGCGGAATGTAGTCGCCTTTTGCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGCCAAGTAGCTCa < 1:233280/139‑1 (MQ=255) gCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTACGCTGAATTTCTGATAAGCCAAGTAGCTCAAGGCATaa > 6:58924/1‑139 (MQ=255) cAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGCCAAGTAGCTCAAGGCATAAGGAACttt < 5:58924/137‑1 (MQ=255) cGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGCCAAGTAGCTCa > 1:43303/1‑113 (MQ=255) cGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGCCAAGTAGCTCa < 2:43303/113‑1 (MQ=255) cGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTAGTACTCAGCCTGAAGGGTTTTAATCCCTTTGACAATTCCGCTGAATTTCTGATAAGCCAAGTAGCTCAAGGCATAAGGAACTTTGATTTAAttt > 6:94480/1‑139 (MQ=255) tCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTGATCCCTTTGCCCCTTCCGCTGAATTTCTGATAAGCCAAGTAGCTCAAGGCATAAGGAACTTTGATTTGATTTGCGCt > 3:158634/1‑139 (MQ=255) tCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTAAGCCTGAAGGGTTTTCATGCCTTTGTCACTTCCGCTGCATTTCTGATAAGCCAAGTAGCTGAAGGCATTAGGAACTTTGATTTGATTTGAGCt > 3:153254/1‑139 (MQ=255) tagCCTTTTCCGTATATTTTTTCGTACTGAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGCCAAGTAGCTCAAGGCATAATGAACTTTGATTTCATTTGAGCTTTTTTCATTTTTTGTat < 4:170251/137‑1 (MQ=255) ccTTTTCCGTATATTTTTTCGTACTAAGCCTGAATGGTTTTAATCCCTTTTACACTTCCGCTGAATTTCTGATAAGCCAAGTTGCTAAAGGCATAAGGAACTTTGATTTGATTTGTGCTTTTTTCATTTTTTGtttata > 2:70712/1‑134 (MQ=255) cTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGCCAAGTAGCTCAAGGCATAAGGAACTTTGATTTCATTTGAGCTTTTTTCATTTTTTGTatcgat < 5:92524/139‑1 (MQ=255) cTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGATGAATTTCTGATAAGCCAAGTAGCTCAAGGCATAAGGAACTTTGATTTCATTTGAGCTTTTTTCATTTTTTGTatcgat < 8:341983/139‑1 (MQ=255) cTTTTCCGTATATTTTTTCGTACACAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGCCAAGTAGCTCAAGGCATAAGGAACTTTGATTTCATTTGAGCTTTTTTCATTTTTTGTatcgat < 4:330510/139‑1 (MQ=255) cTTTTCCGTATAATTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGCCAAGTAGCTCAAGGCATAAGGAACTTTGATTTCATTTGATCTTTTTTCATTTTTTGTatcgat < 1:64809/139‑1 (MQ=255) | CCATTTGCTGTTTTCAAGCTTTTTACGGCGGTTTAACCAAAGGCCGCCTAACGCAGCAAGAATCATGACAACACCTGCTCCTACCATGATGCGGAAGCTCCAGAATGTCGTTTTCACTGGCGGAATGTAGTCGCCTTTTCCGTATATTTTTTCGTACTCAGCCTGAAGGGTTTTCATCCCTTTGACACTTCCGCTGAATTTCTGATAAGCCAAGTAGCTCAAGGCATAAGGAACTTTGATTTCATTTGAGCTTTTTTCATTTTTTGTATCGAT > CP006881/3795964‑3796236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |