BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	121	G→C	5.9%	intergenic (–/‑2857)	– / → eryR	–//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	121	0	G	C	5.9%	5558.9 / 44.9	2002	intergenic (–/‑2857)	–/eryR	–//vntifkey=4
Reads supporting (aligned to +/- strand): ref base G (830/939); new base C (3/149); total (839/1163)
Fisher's exact test for biased strand distribution p-value = 1.20e-34
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2032 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGC  >  Exported/1‑259

gataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAg                                                                                                                                            <  4:267072‑M1/121‑1 (MQ=255)

 ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  6:137501‑M1/123‑1 (MQ=255)

 ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  3:140109‑M1/123‑1 (MQ=255)

 ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTATAATGTCGCAAATATTAATGTTTCTCGTGAACCTTATCAAATATTCGTTCATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  2:69400‑M1/123‑1 (MQ=255)

  taaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          >  2:341200‑M1/16‑138 (MQ=255)

    aatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACgg                                                                                                                                       >  3:338202‑M1/14‑139 (MQ=255)

     atgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACgg                                                                                                                                       <  1:22299‑M1/126‑1 (MQ=255)

     atgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACgg                                                                                                                                       <  4:233132‑M1/126‑1 (MQ=255)

      tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCTTTGATTTAATAGAAACATAGCTGTAAAATTAGCAGTAACTTAATAGAACGGa                                                                                                                                      <  5:81312‑M1/127‑1 (MQ=255)

      tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaa                                                                                                                                     >  6:250778‑M1/12‑139 (MQ=255)

      tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGa                                                                                                                                      <  8:229051‑M1/127‑1 (MQ=255)

       gcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaaa                                                                                                                                    <  1:121046‑M1/129‑1 (MQ=255)

       gcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaaa                                                                                                                                    <  8:203343‑M1/129‑1 (MQ=255)

         ttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAAt                                                                                                                                   <  7:210550‑M1/130‑1 (MQ=255)

         ttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAAt                                                                                                                                   <  7:229142‑M1/130‑1 (MQ=255)

           caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                 <  1:29865‑M1/132‑1 (MQ=255)

             ataaTCCATCATCCAAAGTGGGTGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTGATTAAATAGAAACATAGCGGTAAAATTAGCATTAACTTAATACACCGGAAATCaaaa                                                                                                                              >  8:55552‑M1/5‑139 (MQ=255)

                   cagcctcaAACGTTGGAGAGTTATTTTTATCTCGAAAATATTAATGTTTCTGGTGAACATTATAAAATTTTCGTTTATTAAATATAAACATCGCGTTAAAATTACCAGAAAATAAATCGAACGTAAATCAAAAACGCCa                                                                                                                        <  4:64673/136‑1 (MQ=255)

                   caacctccAACGTTGCAGAGTGAGTTTTATGCCCCAAATATTAATGTTTCTGGTTAACCTTATCAAATATTCGTTGATTAAATAGAAACAAAGCGGTAAAATTCGCAGTAACTAAATAGAACGGAAATGAAAAACGCCa                                                                                                                        <  2:241590/136‑1 (MQ=255)

                   cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  7:26068/139‑1 (MQ=255)

                   aaTCCTCAAAAGGTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  4:204700/138‑1 (MQ=255)

                     tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTGTGGTGAACCTTATAAAATTTTCTTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      <  7:33114/139‑1 (MQ=255)

                     tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      >  2:289949/1‑139 (MQ=255)

                     tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      >  2:89750/1‑139 (MQ=255)

                     tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      <  3:120268/139‑1 (MQ=255)

                     tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATAAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      >  7:36507/1‑139 (MQ=255)

                     tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATAAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      <  5:160063/139‑1 (MQ=255)

                          cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAAAGTTTCTGGTGAACCTTATCAAATTTACGTTGATTTAATAGAAACATAGCGGTAAAATTAGGAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCa                                                                                                                  >  5:184316/1‑138 (MQ=255)

                           aaaGTTGTAGAGTGAGTTTTTTGTCGAAAATATTAATGTTTCTGGTGAACATTATAAAATTTTCGTTTATTTAATACAAAAATAGCGTAAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCata                                                                                                                <  8:254751/139‑1 (MQ=255)

                                  gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        >  4:169677/1‑124 (MQ=255)

                                  gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                         >  7:346312/1‑139 (MQ=255)

                                  gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                         >  4:351152/1‑139 (MQ=255)

                                       tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACtctc                                                                                                                   <  4:287726/124‑1 (MQ=255)

                                        gagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATAAGCAGTAACTTAATAGACCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                   <  1:101357/139‑1 (MQ=255)

                                         agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                         >  5:101219/1‑132 (MQ=255)

                                         agtTTTATGTCGCAAATATAAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCATTAACTAAAAAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  1:73025/139‑1 (MQ=255)

                                          gtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATACAAACATCGCGGTAAAATTAGCAGTAACTAAATACACCGCAAATCAAAAACGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  6:31123/138‑1 (MQ=255)

                                                   cGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                        >  1:14298/1‑139 (MQ=255)

                                                     cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGTCACTCTCata                                                                                                                <  3:361492/113‑1 (MQ=255)

                                                     cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGctg                                                                                      >  1:249592/1‑137 (MQ=255)

                                                     cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                        >  1:4900/1‑137 (MQ=255)

                                                        atcttAATGTTGCTGGAGAACCTTATCAATATATCGTTTATTTAATAGAAACAAAGCGGTAAAATAATCAGTAACTTAATCGAACGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  5:262948/136‑1 (MQ=255)

                                                        atatTTATGTTTCTGGTGAACATTAAAAAATTTTCGTTCTTTTAATAGAAACATAGCGTTAAAATTAGCAGAAAATTAATACACCGCAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  1:164771/139‑1 (MQ=255)

                                                        atatTAATGTTTCTGGTGACCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  6:166628/139‑1 (MQ=255)

                                                        atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  3:285915/139‑1 (MQ=255)

                                                        atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  7:75165/139‑1 (MQ=255)

                                                        atatAACTGTTTCTGGTGAACCTTAACAAATTTTCGTTGATTTAATAGAAAAATAGCGTTAAAATTAGCTGTAACTTAATAGACCGCAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  5:284420/139‑1 (MQ=255)

                                                           ttAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGATCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGa                                                                                >  7:59919/1‑139 (MQ=255)

                                                              ttGTTTGTGGTTAACATTATAAATTTTTCTTTTATTTAATATAAAAATAGCGGTAATATTAGCATTAAATTAATAGACCGAAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                              <  3:49594/137‑1 (MQ=255)

                                                              ttGTTTCTGCTCAACTTAAAAAAATTTTCGTTCATTAAAAAAAAAAAAAGCGTAAAAATTACCATAAACTTAATACAACGCAAATAAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                              <  5:55461/137‑1 (MQ=255)

                                                              atttttCTGGTCAACATTATAAAATTTTCTTTTATTAAATATAAAAATAGCGGAAAATTTAGCATTAACATAATCTAACGTAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                              <  8:129944/135‑1 (MQ=255)

                                                              aTGTTTCTGGGTAAACTTATAAAATTTTGTTTTATATAATAGAAAAATAGCGGTAAAATTAGCATTAACTAAATCCACCGCAAATTAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                              <  6:4566/138‑1 (MQ=255)

                                                                 tttCTGGTTAACCTTATCAATTTTTCTTTTTTTTAATATAAACATAGCGGAAAAATACGATTAAACTAAATATAACGTAATTGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                           <  2:127790/138‑1 (MQ=255)

                                                                 tttCTGGTTAACCTTATCAAATTTTCGTTGATTTAATAGAAAAATAGCGTTAAAATTAGCAGTAAATTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                           <  5:332765/138‑1 (MQ=255)

                                                                 attCGGGTTAACCTAAACAAATTTTCGTTGATTAAAAAGAAACATAGCGCTAAAATTCGCAGTAACTTCAAACCACGCAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                           <  4:112690/137‑1 (MQ=255)

                                                                   tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        >  2:307161/1‑91 (MQ=255)

                                                                   tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  8:75856/113‑1 (MQ=255)

                                                                   tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  5:211430/1‑139 (MQ=255)

                                                                   tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  3:160982/1‑139 (MQ=255)

                                                                   tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  3:239239/1‑139 (MQ=255)

                                                                   tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  7:164026/1‑139 (MQ=255)

                                                                    cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  7:307535/90‑1 (MQ=255)

                                                                    cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                       >  5:354090/1‑139 (MQ=255)

                                                                       gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    <  4:318913/139‑1 (MQ=255)

                                                                       gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    <  6:70329/139‑1 (MQ=255)

                                                                        tGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTAc                                                                   <  3:308685/139‑1 (MQ=255)

                                                                         gAACCTTATCAAATATACGATGATTTAATAGAAACAAAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATTCTATTGGCTACCAACATTTAGAGAGAATGACTAAATCCTGTAc                                                                   >  6:201305/1‑138 (MQ=255)

                                                                          aaCCTTATATATTTATCGTTGTATTATAATAATCATAGTGTTAAATTTAGCAGATACTTATATCCCCGGAAAAGAAAAAAGCCTCTCTCATATGCTATTGGCGACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                 <  2:215266/139‑1 (MQ=255)

                                                                          aaCCTAACCAAATTTGCGTTTATTTAATATAAACATTGCGTTAAAATTACCAGAAACTTAATAGAACCGAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                 <  6:105856/139‑1 (MQ=255)

                                                                           aCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                 <  2:47525/138‑1 (MQ=255)

                                                                           aCCTTAACATATTTTCGTTGATTTAATAGAAAAAAAGCTGTAAATTTAGAAGTAACTTAAAATAAGGTAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGc                                                                <  1:275284/139‑1 (MQ=255)

                                                                            ccTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCATGTACAGc                                                                >  5:241519/1‑138 (MQ=255)

                                                                               tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  3:206644/79‑1 (MQ=255)

                                                                               tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                      >  6:283078/1‑129 (MQ=255)

                                                                               tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                            >  7:145175/1‑139 (MQ=255)

                                                                               tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGATATTGGCTACCACCCTTTAGCGAGAATGACTTAATCATGTACAGCCAtc                                                            >  7:96905/1‑138 (MQ=255)

                                                                                 tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                           <  7:117263/122‑1 (MQ=255)

                                                                                 tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    <  5:134635/129‑1 (MQ=255)

                                                                                      ttttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                 >  4:147795/1‑127 (MQ=255)

                                                                                       tttCGTTGATTTAATATAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCATCCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                    >  2:161663/1‑139 (MQ=255)

                                                                                       tttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                    >  1:189374/1‑139 (MQ=255)

                                                                                        ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTCCCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                                   >  8:153895/1‑139 (MQ=255)

                                                                                        ttCGTTGATTTAATAGAAACATAGCGGTAAAATTACCATTAACTTAATACAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                    <  2:1074/138‑1 (MQ=255)

                                                                                         tCGTTGATTTAATAGAAACATAGCGGTAAAATTAGAAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                                   <  6:88530/138‑1 (MQ=255)

                                                                                               aTTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGACCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACAAAACTTTATCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTta                                             >  3:187438/1‑138 (MQ=255)

                                                                                                         aaCATAGGGGAAAAATTAGCAGTAACTAAAAAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                  <  2:265054/139‑1 (MQ=255)

                                                                                                         aaCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                  <  8:179583/139‑1 (MQ=255)

                                                                                                          aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    >  8:285526/1‑104 (MQ=255)

                                                                                                          aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                  >  2:189739/1‑138 (MQ=255)

                                                                                                          aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                  >  8:301110/1‑138 (MQ=255)

                                                                                                          aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCa                                 >  3:26589/1‑139 (MQ=255)

                                                                                                              aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCACCCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                             >  1:35144/1‑139 (MQ=255)

                                                                                                                   tAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                         >  1:327917/1‑138 (MQ=255)

                                                                                                                          aGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAAACCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt                 >  7:188475/1‑139 (MQ=255)

                                                                                                                               tAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGcc            >  5:309755/1‑139 (MQ=255)

                                                                                                                               tAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGcc            <  7:28809/139‑1 (MQ=255)

                                                                                                                                 aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt          >  6:339452/1‑139 (MQ=255)

                                                                                                                                 aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt          >  3:83253/1‑139 (MQ=255)

                                                                                                                                     aaTAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGttt      >  8:356767/1‑139 (MQ=255)

                                                                                                                                       tAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                                   >  8:334473/1‑92 (MQ=255)

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGC  >  Exported/1‑259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`