BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	137	A→C	5.8%	intergenic (–/‑2841)	– / → eryR	–//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	137	0	A	C	5.8%	5260.9 / 59.0	2090	intergenic (–/‑2841)	–/eryR	–//vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (1001/924); new base C (2/163); total (1003/1087)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2173 total aligned reads displayed.

TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCTTATTA  >  Exported/1‑274

tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                                         >  7:207607/1‑139 (MQ=255)

  catcatCCAAATTTGGAGAGTGAGTTTTATTTCGAAAATATTAATTTTTTTGTTGAACCTTATCAAATTTTCGTTTATTTAAAAGAAACATAGGGGTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAACGCCa                                                                                                                                       <  5:248992/139‑1 (MQ=255)

  cATCCTCCAAAGTTGTAGAGTGATTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAATTTTTCGTTGATTTAATATAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAACGCCa                                                                                                                                       <  5:270259/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                       <  5:363093/139‑1 (MQ=255)

  cATCCCCCAAAGTTGGAGAGTGAGTTTTATGTCCAAAATATTAATGTTTCTGGTCAACCTTATCAAATTTTCGTTGATTTAAAACAAACATAGCGGAAAAATTACCAGTAACTTAATACAACGCAAAGCAAAAAAGCCa                                                                                                                                       <  4:53908/139‑1 (MQ=255)

   ttcctccAAAGTTGTAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTCAACCTAATAAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTACCATTAACTTAATATAAGGGAATTGAAAAAAGCCAc                                                                                                                                      <  7:341963/138‑1 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                                     >  2:284545/1‑139 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                                     >  5:62324/1‑139 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                                     >  5:196849/1‑139 (MQ=255)

     cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCActc                                                                                                                                    >  2:182903/1‑139 (MQ=255)

         cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCa                                                                                                                                 >  1:318391/1‑138 (MQ=255)

         cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGAGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAATCCACTCTCa                                                                                                                                 >  7:226037/1‑138 (MQ=255)

          aaaGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCata                                                                                                                               <  3:156998/139‑1 (MQ=255)

                ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGCACCTTATCAAATTTTCGTTGATTTAATAGAAACATATCTTTAATATTAGAAGTAACTTAATAGAACGGAATAGAAAAAAGCCACTCTCATATGCTAt                                                                                                                         >  3:199757/1‑139 (MQ=255)

                ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                       <  7:255175/125‑1 (MQ=255)

                ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAt                                                                                                                         >  6:341235/1‑139 (MQ=255)

                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGTTTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGATATGAAAAAAGCCACTCTCATATGCtttt                                                                                                                        >  1:90820/1‑139 (MQ=255)

                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAATTGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                        >  2:11177/1‑139 (MQ=255)

                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                        >  1:259301/1‑139 (MQ=255)

                      tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGCACCTTATCAAATTTTCGTTGATTTAATAGCAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                                   >  7:124419/1‑139 (MQ=255)

                      tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                                   >  2:38994/1‑139 (MQ=255)

                       gagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATAAGCAGTAACTTAATAGACCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                  <  1:101357/139‑1 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCata                                                                                                                               <  5:335117/125‑1 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                  >  4:285915/1‑138 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                  >  3:66898/1‑138 (MQ=255)

                         gtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATACAAACATCGCGGTAAAATTAGCAGTAACTAAATACACCGCAAATCAAAAACGCCACTCTCATATGCTATTGGCTAcc                                                                                                                 <  6:31123/138‑1 (MQ=255)

                             tATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCt                                                                                                            >  1:342248/1‑139 (MQ=255)

                                  cGCAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTAGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTAAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                       >  7:356572/1‑139 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                       >  2:301007/1‑137 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                       >  1:146600/1‑137 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTTTCAAATTTTCGTTGATTTATTATAAACATAGGGTTAAATTTAGCAGAAACTTATTAGAACGGCAATGAAAACAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                  <  3:230197/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATATAAACATAGCGGTAAAATTAGCAGAAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                  <  5:90415/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                  <  3:351152/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                  <  7:67470/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAAATTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                  <  1:229686/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATAAATTTTTCGTTGATTTATAAGAAACATAGCGGTAAAATTAGCTGAAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                  <  2:257722/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGGGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGAAAAATTAGCAGTAACTTAATAGAACGGAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                  <  3:27860/139‑1 (MQ=255)

                                        tattatTGTTTGTGTTCACCTTTATAAAATTTTCGTTGATTTAATAGAACAATAGCGGTAAAATTATAAGTAACTTAATAGAACGTAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                  <  8:283655/138‑1 (MQ=255)

                                             ttGTTTATGGTTAACATTAACAAATTTTCTTTTATTAAATAGAAAAATCGCGTTAAAATTAGCAGAAAATTAAAACAACGCAATTTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                             <  1:192720/137‑1 (MQ=255)

                                             aTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACtt                                                                                            <  1:44394/139‑1 (MQ=255)

                                             aTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                             <  6:64938/138‑1 (MQ=255)

                                                tttCTGGTCAACCTAATCAAATTTTCTTTGATTTAAAAGAAACATAGCGTAAAAATTAGCAGTAACTTAATACAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                          <  6:120807/138‑1 (MQ=255)

                                                tttCTGGTCAACATTATAAAATTTTCGTTGATTAAATAGAACAAAAGCGTTAAAATTCGCAGAAAATTAATAGAACGTAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                          <  4:309402/138‑1 (MQ=255)

                                                tttATGGTTATCATTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATAATCAGTAAATTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                          <  8:169265/138‑1 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                                 >  8:33034/1‑113 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                                        <  7:3241/138‑1 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGATATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                       >  3:30330/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAATCTCATATGCTATTGCCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                       >  5:175271/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATAAAATTTTCGTTTATTTAATAGAAACATAGCTTTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTTATATGCTATTGGAAACAAACCTTAAGCGAGAATTACTTAATc                                                                                        >  2:332337/1‑138 (MQ=255)

                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                        >  6:282955/1‑105 (MQ=255)

                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                                      >  3:183710/1‑139 (MQ=255)

                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACAAACCTTTAGCGAGAATGACTTAATcac                                                                                      >  3:322504/1‑137 (MQ=255)

                                                    ttgTGAACCTAATAAAATTTTCTTTTATTTATTAGAAACAAAGCGGAAAAATTAGAAGTAACTTAATAGAAGTGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                                     <  8:226037/137‑1 (MQ=255)

                                                    tGGTTAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCGTTAAAATTAGCATTAACTTAATAGACCGGAAATAAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                                     <  6:205072/139‑1 (MQ=255)

                                                      gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                   <  3:132793/139‑1 (MQ=255)

                                                      gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                   <  8:119542/139‑1 (MQ=255)

                                                      gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAG‑CACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                   <  4:26169/138‑1 (MQ=255)

                                                      gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                   <  7:287537/139‑1 (MQ=255)

                                                       tGAACCTTATCAAATTTTCGTTGATTTAATATAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTAc                                                                                  <  2:61120/139‑1 (MQ=255)

                                                        gAACCTTATCAATTTTTCGTTGATTTAATATAAACATAGCTTTAAAATTAGCATTAATTTATTATAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTAc                                                                                  <  5:115236/138‑1 (MQ=255)

                                                         aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGAACAg                                                                                <  8:250796/139‑1 (MQ=255)

                                                               aTCAAAATTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCactg                                                                          >  7:18549/1‑136 (MQ=255)

                                                                tCAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACa                                                                         <  1:127731/139‑1 (MQ=255)

                                                                tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                           >  6:95659/1‑137 (MQ=255)

                                                                       ttCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGATACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                   <  6:148914/138‑1 (MQ=255)

                                                                       ttCGTTTATTTAATACAAAAATAGCGGAAAAATTAGCATTAACTTAATAGAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                   <  1:284545/138‑1 (MQ=255)

                                                                        tCGTTGATTTAATACAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                                                  <  7:14907/138‑1 (MQ=255)

                                                                          gTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGAc                                                               >  3:44875/1‑139 (MQ=255)

                                                                              aTTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTta                                                            >  1:69068/1‑138 (MQ=255)

                                                                                         aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                                 >  8:301110/1‑138 (MQ=255)

                                                                                             aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                                            <  8:192086/139‑1 (MQ=255)

                                                                                              gagttAAAATTATCAGTAACTTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                                            <  8:77791/134‑1 (MQ=255)

                                                                                                 gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                   <  4:224457/112‑1 (MQ=255)

                                                                                                 gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                                        >  3:256569/1‑139 (MQ=255)

                                                                                                   aaaaTTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATa                                      <  8:146032/139‑1 (MQ=255)

                                                                                                     aaTTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAg                                    >  2:23531/1‑139 (MQ=255)

                                                                                                          gCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt                                <  4:156804/138‑1 (MQ=255)

                                                                                                            aGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCTCCAACTTCAAATAAGTTATTTg                             >  2:85594/1‑139 (MQ=255)

                                                                                                              tAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGcc                           >  5:309755/1‑139 (MQ=255)

                                                                                                               aatatAATACAACCGAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGcc                           <  4:191355/134‑1 (MQ=255)

                                                                                                               aaCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGcc                           <  2:204947/138‑1 (MQ=255)

                                                                                                                aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt                         >  7:91246/1‑139 (MQ=255)

                                                                                                                aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt                         >  7:49364/1‑139 (MQ=255)

                                                                                                                    aaTAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGttt                     >  8:356767/1‑139 (MQ=255)

                                                                                                                        gAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgc                 >  2:143440/1‑139 (MQ=255)

                                                                                                                        gAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgc                 >  7:301639/1‑139 (MQ=255)

                                                                                                                         aaCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                          <  6:249887/65‑1 (MQ=255)

                                                                                                                          aCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgcg                >  5:28971/1‑138 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                  >  8:58105/1‑55 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa              >  4:90722/1‑139 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa              >  3:112985/1‑139 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa              <  5:220102/139‑1 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa              >  6:178172/1‑139 (MQ=255)

                                                                                                                              aaaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAAc             >  1:171799/1‑137 (MQ=255)

                                                                                                                                    aaaacGCCACTCTGATATGCTATTGGCTACCTAGCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCtta     <  4:129873/139‑1 (MQ=255)

                                                                                                                                    aaaaaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCtta     <  4:345531/139‑1 (MQ=255)

                                                                                                                                    aaaaaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCtta     <  6:364475/139‑1 (MQ=255)

                                                                                                                                       aaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                   >  5:146124/1‑74 (MQ=255)

                                                                                                                                        aGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCttat    >  3:95748/1‑136 (MQ=255)

                                                                                                                                        aGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCttat    >  7:165063/1‑136 (MQ=255)

TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCTTATTA  >  Exported/1‑274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`