BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,741	G→T	9.5%	intergenic (+26/‑130)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,741	0	G	T	9.5%	4339.8 / 144.3	1485	intergenic (+26/‑130)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base G (43/1269); new base T (145/21); total (192/1293)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1526 total aligned reads displayed.

ATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTAT  >  Exported/3603‑3878

 tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTgg                                                                                                                                           <  7:97646/139‑1 (MQ=255)

  cAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                          <  8:220919/139‑1 (MQ=255)

  cAGTTTCAACAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                          <  3:36381/139‑1 (MQ=255)

   attttCTTCAACAAATAAAAAACGCAAAAGTAAACAATTAAACTACAATTAATTATGAGCAATAATTGTCTATTTTTAATAGTTATCTATTATTTACCGGGATGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTgta                                                                                                                                          <  4:223063/136‑3 (MQ=255)

   agattCATCCAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                          <  5:176775/135‑1 (MQ=255)

   aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                          <  6:2147/138‑1 (MQ=255)

          tcaatcaaTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                  <  8:14739/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                  <  6:150117/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                  <  6:359237/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                  <  5:257015/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                  <  4:275627/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                  <  3:62864/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                  <  2:41227/139‑1 (MQ=255)

          acaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                  <  7:336270/138‑1 (MQ=255)

            aaGCAATTAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                <  5:13106/139‑1 (MQ=255)

            aaGCAATTAAACAAGCCAAAGTAAACAATTTAAGTACCATTTCTTATGAGCAAGGATTTTCTATTTTTAATATTTATCTATTATTTAACTGTAGGAAATATATCTATGAGTCTC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                <  7:265827/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                <  3:218747/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                <  3:337693/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                <  6:124419/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                <  2:118567/139‑1 (MQ=255)

               cAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTa                                                                                                                             <  2:338104/139‑1 (MQ=255)

                 aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                           <  8:198538/139‑1 (MQ=255)

                 aTGAAACACGACAAACTAAACAATTTAAGTACCATTACATATGAGCAAGTATTTTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCTC‑TTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                           <  6:186991/139‑1 (MQ=255)

                    aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGTGCAAGTATTGTCTATTTTTATTAGTTATCTATTATTTATCGGGAGGAAATTATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTaa                                                                                                                         <  3:247620/138‑1 (MQ=255)

                    aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                        <  2:213303/139‑1 (MQ=255)

                    aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                        <  1:358126/139‑1 (MQ=255)

                    aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                        <  8:35526/139‑1 (MQ=255)

                     aaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                        <  2:29593/138‑1 (MQ=255)

                         cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                   <  6:250325/139‑1 (MQ=255)

                         cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                   <  5:229736/139‑1 (MQ=255)

                         cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                   <  5:277779/139‑1 (MQ=255)

                                 tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                           <  7:181196/139‑1 (MQ=255)

                                  aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                           <  2:227668/138‑1 (MQ=255)

                                   aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                         <  6:283077/139‑1 (MQ=255)

                                    aCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTTACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGAGAGTTACAACTTAGATAATGGTATGGAGATAAat                                                                                                        >  4:134231/1‑139 (MQ=255)

                                     cAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAatt                                                                                                       <  2:58301/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                    <  7:54001/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                    <  6:100646/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGGTTTTTTATTTTTGTATTTTA‑AAGTTAAAAAATTGAATTGATATAAattatt                                                                                                    >  1:329768/1‑139 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                    <  3:294164/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                    <  5:327798/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                    <  5:45958/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                    <  7:116083/139‑1 (MQ=255)

                                        tttAAGTACCAATAATTATGAGCATGTATTGTCTTTTTTAAATAGTTATCTATTATATATCGGGAGGAATTAATTCTATGAGTCGC‑TTTTTTAAATTTGGAATGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                    <  5:154281/139‑1 (MQ=255)

                                           aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                  <  6:305533/138‑1 (MQ=255)

                                            aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                <  8:113954/139‑1 (MQ=255)

                                            aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                <  3:173928/139‑1 (MQ=255)

                                            aGTACCATTACTTATGAGCAAGTATTGTCTATATATAATAGTTATCTATTATATAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTATATTTGGAAAGTTACATGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                <  1:157672/139‑1 (MQ=255)

                                             gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                               <  3:132927/139‑1 (MQ=255)

                                             gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                               <  1:302619/139‑1 (MQ=255)

                                             gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGCGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                               <  1:177628/139‑1 (MQ=255)

                                                      cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                      <  7:359211/139‑1 (MQ=255)

                                                      cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                      <  1:227116/139‑1 (MQ=255)

                                                         aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                     <  4:58710/137‑1 (MQ=255)

                                                           gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAAATTT‑GAAAATTAAAAGTTAATAAATGGTATTGAGATAAAATATTTGATAAAAAAATGACAGCt                                                                                 >  6:70012/1‑139 (MQ=255)

                                                           gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTTGGAATTTTAACGTTAATAAAAGGTATTGAGATAAATTTTTATATATAAAAATTACAGCt                                                                                 >  8:49175/1‑139 (MQ=255)

                                                               aattATTGTCTATTTTTAATAGTTATCTATTATTAAACGTGAGGAAATAATTCTATGAGTCGC‑TTATTTAAATTTGGAAAGTTACACGTTACTAAAGGCAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                             <  4:156889/136‑1 (MQ=255)

                                                               aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                             <  5:350538/139‑1 (MQ=255)

                                                               aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                             <  8:171234/139‑1 (MQ=255)

                                                               aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                             <  1:85029/139‑1 (MQ=255)

                                                                 gtctagTCTATTTTTAATAGTTATCTAATAATTAACGGGAGGAAATAATTCTAAGAGTCGC‑TTGTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                           <  7:80452/134‑1 (MQ=255)

                                                                 gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                           <  1:6279/139‑1 (MQ=255)

                                                                 gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAAATCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                           <  7:227413/139‑1 (MQ=255)

                                                                       tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTa                                                                     <  2:256602/139‑1 (MQ=255)

                                                                         gaTTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                   <  1:10028/138‑1 (MQ=255)

                                                                           tttttAATAGTTATCTATTATTTAACTGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                   <  7:161064/137‑1 (MQ=255)

                                                                           tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                   <  1:13162/137‑1 (MQ=255)

                                                                            ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTTATATTTTCACGTTTATAAAGGGAATGGAGATTAATTATTTGATATACTACTGACAGCTTCCAAGAAGATAAag‑at                                                               >  5:65892/1‑138 (MQ=255)

                                                                             tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag‑ag                                                               <  2:15407/138‑1 (MQ=255)

                                                                             tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag‑ag                                                               <  5:200001/138‑1 (MQ=255)

                                                                             tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag‑ag                                                               <  4:110977/138‑1 (MQ=255)

                                                                             tttAATAGTTATATATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCAAAGAAGCTAAag‑ag                                                               <  1:192468/138‑1 (MQ=255)

                                                                                      tATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTTTAATTTAAACGTTAATAAATGGAATTGTGATAAATTATTATATATAATACTTACAGCTTACAAGAAGCTAAAG‑AGGTCCCTAgcg                                                     >  2:93433/1‑139 (MQ=255)

                                                                                              attTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACgg                                              <  4:246887/138‑1 (MQ=255)

                                                                                               tttATCGGGAGGAAATAATTCTATGAGTCGG‑TTTTTTAAATTTGGAAAGTTACACGTTACTTAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACggg                                             <  8:76267/138‑1 (MQ=255)

                                                                                               tttAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAACGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACggg                                             <  7:283027/138‑1 (MQ=255)

                                                                                               tttAACGGGAGGAAATAATACTCTTCGTCGC‑TTTTTTAAATTTGGACAGTTACACGTTACTAAAGGGAATGGAGATAAATTAATATATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACggg                                             <  3:198776/138‑1 (MQ=255)

                                                                                                       gaggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgg                                    <  6:327681‑M1/139‑3 (MQ=255)

                                                                                                       gaggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAAAGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgg                                    <  8:319488‑M1/139‑3 (MQ=255)

                                                                                                        aggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGggg                                   <  4:338914‑M1/139‑4 (MQ=255)

                                                                                                            aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                               <  8:289485‑M1/139‑8 (MQ=255)

                                                                                                              taatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                             <  7:101428‑M1/139‑10 (MQ=255)

                                                                                                                      tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACTTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                     <  4:305047‑M1/139‑18 (MQ=255)

                                                                                                                      tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATTATTTTTTAGTTATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaatagg                     <  3:82910‑M1/139‑18 (MQ=255)

                                                                                                                      tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                     <  3:315339‑M1/139‑18 (MQ=255)

                                                                                                                      tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                     <  7:204924‑M1/139‑18 (MQ=255)

                                                                                                                       gAGTCGA‑TTTTTTAAATTTTGAAAGTTACACGTTACTAAAGTTAATGGAGATAAATTATTATATATACTACTGAAAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                     <  3:271785‑M1/138‑18 (MQ=255)

                                                                                                                       gAGTCGA‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                     <  4:196626‑M1/138‑18 (MQ=255)

                                                                                                                         ttcGA‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                   <  2:182836‑M1/137‑20 (MQ=255)

                                                                                                                          tCGC‑TTTTTTAATTTTGGTAAGTTACACGTTTCAAAATGGAATTGATATAAATTATTAGATATACTACTGCCAGCTTCCAAGAACCTAAAGCAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaag                  <  2:310449‑M1/139‑21 (MQ=255)

                                                                                                                            gC‑TTTTTTAAATTTGGAAAGTTACAAGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagag                <  7:141163‑M1/138‑23 (MQ=255)

                                                                                                                                 ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat             <  7:240059‑M1/137‑26 (MQ=255)

                                                                                                                                 ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat             <  5:307892‑M1/137‑26 (MQ=255)

                                                                                                                                 ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat             <  5:81960‑M1/137‑26 (MQ=255)

                                                                                                                                 ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTAATAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat             <  3:118018‑M1/137‑26 (MQ=255)

                                                                                                                                     aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta        <  5:106225‑M1/138‑31 (MQ=255)

                                                                                                                                     aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta        <  8:245089‑M1/138‑31 (MQ=255)

                                                                                                                                      aaTTTGGTAAGTTACACTTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAATG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt      <  7:31952‑M1/139‑33 (MQ=255)

                                                                                                                                      aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt      <  1:251243‑M1/139‑33 (MQ=255)

                                                                                                                                      aaTTTGGAAAGATACAGGTTACTTAAGGGAATCGAGATAAATTATTATATATACTACTTACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt      <  1:271356‑M1/139‑33 (MQ=255)

ATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTAT  >  Exported/3603‑3878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`