BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	4,649	A→C	7.8%	Y260S (TAC→TCC) ‡	ampR →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	4,649	0	A	C	7.8%	3165.2 / 45.2	1219	Y260S (TAC→TCC) ‡	ampR	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (829/265); new base C (8/112); total (840/379)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1243 total aligned reads displayed.

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTT  >  Exported/4511‑4786

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                        >  1:138431/1‑139 (MQ=255)

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                        >  2:240056/1‑139 (MQ=255)

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                        >  2:114080/1‑139 (MQ=255)

         cACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACggg                                                                                                                                  >  4:357742/1‑139 (MQ=255)

           cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACggg                                                                                                                                  >  2:348558/1‑137 (MQ=255)

           cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACggg                                                                                                                                  >  7:64625/1‑137 (MQ=255)

                 cgcTCGGCCGTGCGGGGGGGGTGGTTTTTTGCTCATAATTCTGGAGCGGGTGCGGGGGGGTCGCGCGTTATCTTTGCAGCACGGGGGCAATATGGTACCCCCGCCCGTATGTTTTTAATCTACACGACGGGGAGTCAgg                                                                                                                          <  6:60760/139‑1 (MQ=255)

                  gcTCGGCCCTGCGGGCGGGGTGGTTTATTGCTGAAAAATCGGGCGCCGGGGCGGGGGGGTCTCGCGGTATCATTGCCGCCCTGGGGCCCGTTGTAAAGCCCTCCCGTATCTTCGTTATATCCACGACGGGGAGTCAGGc                                                                                                                         <  8:244664/139‑1 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGCGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTACGCCCTCCCGTCTCGTAGTTAGCTACACGACGGTGAGTCAGGCAAc                                                                                                                      >  4:317873/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  2:22965/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  5:252389/1‑138 (MQ=255)

                           ttCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGa                                                                                                                >  3:337955/1‑139 (MQ=255)

                                    gctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGaaa                                                                                                       >  7:85347/1‑139 (MQ=255)

                                              tGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGAGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGTATGAACGAAATAgagaga                                                                                               >  5:280985/1‑137 (MQ=255)

                                              tGCAGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATAATTTCAGCACTGGTTCCAGATGGTAAGCCCTCCCGTATCGTAGTTATATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGAAAGATc                                                                                             >  4:168367/1‑139 (MQ=255)

                                                cttaTAAAGCGGGAGCGGGTGAGGGGGGTTCTCGCGGTATCATTGCTGCCCTGGGGCCAGATGGTAAGCCCGCCGGTATCGTTGTTTTAAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCg                                                                                            <  8:40390/135‑1 (MQ=255)

                                                   aTAAATCTGGAGCCGGTGCGCGTGGGTCTCGGGTTATCATTGCAGCACTGGGGCCTGTTGGTAAGCCCGCCCTTATCGTAGTTATAAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          <  6:149456/137‑1 (MQ=255)

                                                   aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCTGATGTTAAGCCAACCGGTTACGTAGTTATCTACACGACGGGTAGTCAGGCAACTATGGATGAAGGAAATAGAAATATCGGTga                                                                                        >  5:230221/1‑139 (MQ=255)

                                                   aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTga                                                                                        >  7:269384/1‑139 (MQ=255)

                                                        tcgggcgCCGGGGAGCGGGGGGCTCGCGGTATCATTGCCGCACTGGGGCCAGATGGTACGCCCTCCCGTCTCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAg                                                                                   <  7:37232/133‑1 (MQ=255)

                                                          tGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAg                                                                                   >  1:218456/1‑137 (MQ=255)

                                                          tGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGt                                                                                 >  6:130483/1‑139 (MQ=255)

                                                             aGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGcc                                                                              >  7:194812/1‑139 (MQ=255)

                                                                 ggTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCAc                                                                          >  2:179582/1‑139 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGTAGTCAGGCAACTATGGATGAACGAAATAGAAAGATCGCTGAGATAGGTGCCTCACt                                                                         >  5:303534/1‑139 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                                         >  5:102595/1‑139 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGACTCACt                                                                         >  1:234843/1‑139 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCGACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                                         >  3:198282/1‑139 (MQ=255)

                                                                      gCGGGGGGCGCGCGGTATCATACCAGCCCGGGGGCCAGCTGGAAAGCCCTCCCGTCTCGTAGTTAACACCACGACGGGGAGGCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     <  8:20544/139‑1 (MQ=255)

                                                                       cGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     >  5:345213/1‑138 (MQ=255)

                                                                            gTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAt                                                               >  1:3415/1‑139 (MQ=255)

                                                                               tcGAGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCATCCCGAATCGTAGTTATCTACACAACGGGGAGTCAGGCAACTAAGGATGAACGAAATAGACACATCCCTCATATAGTTCCCTCACTGATTAAGAATTgg                                                            >  4:209055/1‑139 (MQ=255)

                                                                                ggctttATCATTGCAGCACTGGGGCCAGTTGGAACCCCCGCGGGTTGGGTCGTAACCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                           <  1:194148/134‑1 (MQ=255)

                                                                                 gcgGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                           >  1:170456/1‑138 (MQ=255)

                                                                                      aTCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                     >  5:93819/1‑139 (MQ=255)

                                                                                       tCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGCCAGATCGCTGAGATAGGTGCCTCACTGATTACGCATTGGTAACTGt                                                     >  6:362631/1‑138 (MQ=255)

                                                                                             cagcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                                               >  3:29786/1‑138 (MQ=255)

                                                                                              agcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                                               >  5:60032/1‑137 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                             <  7:118541/74‑1 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGAATTGGTAACTGTCAGACAAAg                                            >  3:120368/1‑139 (MQ=255)

                                                                                                caCTGGGGCCAGATGGTACCCCCTCCCGTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGt                                           <  2:198669/139‑1 (MQ=255)

                                                                                                 agggggcCCAGATGGTACCCCCCCCCGAGTCGATGTTAACACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          <  8:131115/136‑1 (MQ=255)

                                                                                                 aCTGGGGCATGATGGTACGCCCTCCGGTTTGGTTTTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          <  2:302434/139‑1 (MQ=255)

                                                                                                  cTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          >  1:69873/1‑138 (MQ=255)

                                                                                                  cTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          >  7:153947/1‑138 (MQ=255)

                                                                                                  cTGGGGCCAGATGGTAAGCCCTCCCGTATCCTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGACATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          >  3:266910/1‑138 (MQ=255)

                                                                                                   tGGGGCCTGTGGGTACGCCCGCCCTTTTGGTATTTATAACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  4:61608/139‑1 (MQ=255)

                                                                                                   tGGGGCCAGATGTAAAGCCCTCCCTTATCGTAGTTAACAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  3:319153/139‑1 (MQ=255)

                                                                                                   tGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTaa                                                         >  5:161083/1‑122 (MQ=255)

                                                                                                   tGGGGCCAGAGTGTAAGCCCTCCCGTATCTTTTTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  8:189752/139‑1 (MQ=255)

                                                                                                    ggggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTAc                                       <  5:130483/139‑1 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  1:10291/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  2:74241/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  5:18392/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCATCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCTCTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  6:333769/1‑139 (MQ=255)

                                                                                                     gggCAAGATGGTAACCCCTCCCGTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      <  8:63121/139‑1 (MQ=255)

                                                                                                          ataTGGTAAGCCCACCCTTATCTTATTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                 <  1:273066/137‑1 (MQ=255)

                                                                                                          agttGGTATGCCGTCCGTTTTCGTATTTTTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                 <  8:343116/136‑1 (MQ=255)

                                                                                                          agagGGTACGCCCGCCCTTATCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                 <  2:49909/139‑1 (MQ=255)

                                                                                                          aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                 >  2:136158/1‑139 (MQ=255)

                                                                                                          aGATGGAAAGCCCTCCCGTAGCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                 <  6:165707/139‑1 (MQ=255)

                                                                                                           tttGGTAAGCCCTCCCTTATAGTTTTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata                                <  3:173912/137‑1 (MQ=255)

                                                                                                            aTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatatat                               <  7:286821/139‑1 (MQ=255)

                                                                                                             tGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACAGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatatat                               >  8:260681/1‑138 (MQ=255)

                                                                                                               gTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACt                            >  3:35854/1‑139 (MQ=255)

                                                                                                               gTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGCCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACt                            >  4:241703/1‑139 (MQ=255)

                                                                                                                tAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATAcct                           >  3:154311/1‑137 (MQ=255)

                                                                                                                 aaGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATAcctg                          >  3:99281/1‑136 (MQ=255)

                                                                                                                 aaGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt                           >  1:49909/1‑138 (MQ=255)

                                                                                                                 aaGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAAAGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGCCCAAGTGTACTCATATATACtt                           >  3:52245/1‑138 (MQ=255)

                                                                                                                 aaGCCCTCCCGTATCGAAGTTATCTACACGACAGGGAGTCAGGCAACAATTCAAAAACCAAATAGACAGATCGCTCAGATAGGTGCCTCACTGATAAAGCATTGGTAACTCTCAGACCAAGTTAACTCATATATAcctg                          >  4:342132/1‑136 (MQ=255)

                                                                                                                    cacTCCCGTTTCGTTGTTTTATCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAga                       <  6:266136/137‑1 (MQ=255)

                                                                                                                     ccccccGTATGTTCGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgat                      <  7:21374/136‑1 (MQ=255)

                                                                                                                     ccTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgat                      <  5:146305/139‑1 (MQ=255)

                                                                                                                      cTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgatt                     >  8:316620/1‑139 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     <  8:78968/89‑1 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCata                                  <  2:257466/124‑1 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  7:340944/1‑139 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  4:145791/1‑139 (MQ=255)

                                                                                                                              tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTACCTGTCAGCCCAAGTTTACTCATATATACTTTAGATTGATTTaa              >  7:312304/1‑138 (MQ=255)

                                                                                                                              tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTaa              >  6:307044/1‑138 (MQ=255)

                                                                                                                              tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTaa              >  1:313845/1‑138 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           >  1:253411/1‑74 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTa                                                          <  5:367435/91‑1 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        >  8:23281/1‑109 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTc                                     >  3:70210/1‑112 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt          >  3:236624/1‑139 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt          >  8:158673/1‑139 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAGTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt          >  8:293625/1‑139 (MQ=255)

                                                                                                                                   gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACtt         >  7:229498/1‑138 (MQ=255)

                                                                                                                                   gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCGCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACtt         >  4:277010/1‑138 (MQ=255)

                                                                                                                                   gTTATCTACACGACGGGGAGTCAGGCAACTATGGATCAACGAAATATACAGATCGCTCAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACATTAGATTGATTTAAAACtt         >  4:82003/1‑138 (MQ=255)

                                                                                                                                      aTCTCCACGACGGGGAGTCAGGCCACTATGGATGAACGAAATAGCCAGCTCGCTGAGATAGGTGCCTCGCTGAGTAAGCTTTGGTCACTGTCAGCCCAAGTTTACTCAGAGAGACTTTAGTTTGAGTTAAAACGTCAtt     >  6:24077/1‑139 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                           >  7:251249/1‑69 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                           >  6:205945/1‑85 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt     >  6:86475/1‑139 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt     >  7:175830/1‑139 (MQ=255)

                                                                                                                                       tCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAttt    >  1:87652/1‑139 (MQ=255)

                                                                                                                                        cTACACTACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATTGGTGCCTCACTGATTAATCATTGGTAACTGTCAGACCAAGTTTACTCATATAAAGTTTAGATTGATTTTAATATTCTtttt   >  8:106128/1‑139 (MQ=255)

                                                                                                                                         tACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAtt                                                              <  5:317103/79‑1 (MQ=255)

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTT  >  Exported/4511‑4786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`